BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0115 (593 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g39690.1 68417.m05616 expressed protein 28 5.4 At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 28 5.4 At1g67970.1 68414.m07764 heat shock factor protein, putative (HS... 28 5.4 At3g26730.1 68416.m03342 zinc finger (C3HC4-type RING finger) fa... 27 7.1 At1g18270.1 68414.m02280 ketose-bisphosphate aldolase class-II f... 27 7.1 At1g06460.1 68414.m00684 31.2 kDa small heat shock family protei... 27 7.1 At1g35510.1 68414.m04407 expressed protein contains Pfam PF03138... 27 9.4 At1g14740.1 68414.m01762 expressed protein 27 9.4 >At4g39690.1 68417.m05616 expressed protein Length = 650 Score = 27.9 bits (59), Expect = 5.4 Identities = 25/94 (26%), Positives = 42/94 (44%), Gaps = 4/94 (4%) Frame = -2 Query: 571 EXELGTLQTIPSPDIELSYIRLRSRDARVKKKTDSIDLRDPNGLRRRVSRFECETR---- 404 E E L+ +P P + +S + +T+S +DP L+ E E + Sbjct: 217 EAESVKLEAVPKPGDSPIIVNAQSSSVHRESETESASPKDPAALKTPEDGIEREVQLPGS 276 Query: 403 LVKSHCLEPPDSRGSTVSISLPDSARLASALEAF 302 L+K + LE D+ ST S S+ + ++ EAF Sbjct: 277 LLKEYNLEGSDTE-STGSSSIGE--QITKETEAF 307 >At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4 other kinesin-like proteins of A. thaliana: F02P16.12 (PID:g2191180), katA (D11371), katB (D21137), and katC (D21138); contains non-consensus AT-AC splice sites at intron 10 Length = 1087 Score = 27.9 bits (59), Expect = 5.4 Identities = 17/39 (43%), Positives = 20/39 (51%) Frame = -1 Query: 245 PNVRNCGSSRTEQYYYRNDKPSVG*N*PVSRRSNPAHVP 129 P+ R+ + R RN KP V N PVSR PA VP Sbjct: 829 PSRRSLSTDRASTIKSRN-KPDVTQNLPVSRTPFPARVP 866 >At1g67970.1 68414.m07764 heat shock factor protein, putative (HSF5) / heat shock transcription factor, putative (HSTF5) identical to heat shock transcription factor 5 (HSF5) SP:Q9S7U5 from [Arabidopsis thaliana]; contains Pfam profile: PF00447 HSF-type DNA-binding domain Length = 374 Score = 27.9 bits (59), Expect = 5.4 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +3 Query: 264 GRWCELPSAGLCLNASKAEASLAESGKDMLTVEPRESGGSKQCD 395 G W +L +K + ++ + GKD LT+E E G+ + D Sbjct: 286 GAWEKLLLLSPSRKKTKKQENIVKKGKDDLTLEEEEEDGTMELD 329 >At3g26730.1 68416.m03342 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 772 Score = 27.5 bits (58), Expect = 7.1 Identities = 16/45 (35%), Positives = 22/45 (48%) Frame = -2 Query: 370 SRGSTVSISLPDSARLASALEAFRHNPADGSSHHRPLGRCMNQMS 236 SRG + S++L F ++P G SHH P GR N +S Sbjct: 119 SRGRGTGAAGSSSSQLQHNSGPF-NSPRGGVSHHNPTGRRANMIS 162 >At1g18270.1 68414.m02280 ketose-bisphosphate aldolase class-II family protein low similarity to KbaY (tagatose-1,6-bisphosphate aldolase) [Escherichia coli] GI:8895753; contains Pfam profile PF01116: Fructose-bisphosphate aldolase class-II Length = 1373 Score = 27.5 bits (58), Expect = 7.1 Identities = 20/72 (27%), Positives = 31/72 (43%), Gaps = 6/72 (8%) Frame = +3 Query: 192 VAIVILLSTRGTAVSDIWFMHR------PSGRWCELPSAGLCLNASKAEASLAESGKDML 353 V +++++ T D+ + H PSG L S S+ E L GKD+ Sbjct: 378 VDVLVIMVTNEVQAEDVLYGHLGAVEAIPSGATVVLASTVSPAFVSQLERRLENEGKDLK 437 Query: 354 TVEPRESGGSKQ 389 V+ SGG K+ Sbjct: 438 LVDAPVSGGVKR 449 >At1g06460.1 68414.m00684 31.2 kDa small heat shock family protein / hsp20 family protein contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 285 Score = 27.5 bits (58), Expect = 7.1 Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%) Frame = +1 Query: 313 RPKPA*PNPARICSLWSPESREALNNVTLLVAFRIQNAR-RDVEAHLD 453 RPK P A + ++WSP S A + + +VA + A D+ +D Sbjct: 169 RPKLDLPKLANLGTVWSPRSNVAESTHSYVVAIELPGASINDIRVEVD 216 >At1g35510.1 68414.m04407 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'growth regulator protein -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 568 Score = 27.1 bits (57), Expect = 9.4 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = -3 Query: 72 ADIEGSKSNVAMNAWLPQ 19 AD+ GS NV M AW P+ Sbjct: 122 ADVNGSSHNVLMEAWKPR 139 >At1g14740.1 68414.m01762 expressed protein Length = 733 Score = 27.1 bits (57), Expect = 9.4 Identities = 16/58 (27%), Positives = 29/58 (50%), Gaps = 2/58 (3%) Frame = +3 Query: 276 ELPSAGLCLNASKAEASLAESGKDMLTVEPRESGGSK--QCDFTSRVSHSKRETRRRS 443 EL + LC N+ K SL + GK+++T S K + DF + + ++R++ Sbjct: 69 ELTLSYLCENSGKLAESLGQKGKEVVTFSENSSYDDKWVERDFFNLREMNPNSSKRKA 126 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,102,394 Number of Sequences: 28952 Number of extensions: 273950 Number of successful extensions: 702 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 688 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 702 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1180950720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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