BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20898 (609 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 27 1.6 SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces ... 27 2.1 SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyc... 26 3.7 SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces pom... 26 3.7 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 5.0 SPAC1F12.05 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.0 SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr ... 25 6.5 SPAC26H5.12 |rpo41||mitochondrial DNA-directed RNA polymerase|Sc... 25 8.7 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 27.5 bits (58), Expect = 1.6 Identities = 17/52 (32%), Positives = 29/52 (55%), Gaps = 8/52 (15%) Frame = +1 Query: 256 MDIDRVEEQEMPLLKSELLHC-----QEYGNEII--AFTDN-EFKTINNPFG 387 +DID + +P + +++ C QEY E+I AF+DN +T++ FG Sbjct: 182 LDIDTYRDHVLPAIIEQIIECRDSLAQEYLVEVICQAFSDNMHLQTLDTYFG 233 >SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces pombe|chr 3|||Manual Length = 607 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 2/54 (3%) Frame = +2 Query: 95 LIFEIGNNVSIGISIYKLLK--SNIEPKKVYLSNETNAVVTSDTKTTKVLVKPD 250 L ++GN+V IG+ + LLK + + VY E AV +K K + Sbjct: 239 LKMDLGNDVRIGVEAFILLKRLESAKTNWVYAKGERFAVAVPQSKQVSFATKKE 292 >SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 581 Score = 26.2 bits (55), Expect = 3.7 Identities = 27/84 (32%), Positives = 37/84 (44%) Frame = +2 Query: 32 KIEDVTKLILNECDRHLATAQLIFEIGNNVSIGISIYKLLKSNIEPKKVYLSNETNAVVT 211 KIE T + + D+ + + I+E+ + + LK E V LSNE N +T Sbjct: 47 KIESSTLEVQKKKDQ-IRELERIYEMNPKSFLTLRRTPALKLLPETLSVELSNEVN--LT 103 Query: 212 SDTKTTKVLVKPDTVWILIGLKNK 283 S T T VKP LKNK Sbjct: 104 SST--TSSCVKPSPYLTNCNLKNK 125 >SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 26.2 bits (55), Expect = 3.7 Identities = 17/47 (36%), Positives = 25/47 (53%) Frame = +2 Query: 38 EDVTKLILNECDRHLATAQLIFEIGNNVSIGISIYKLLKSNIEPKKV 178 ED+ KL L+ECD I+ +G N + +L+K +EP KV Sbjct: 379 EDLEKLDLSECD--------IYMLGPNNYMRFVKQELVKLGVEPNKV 417 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 25.8 bits (54), Expect = 5.0 Identities = 14/57 (24%), Positives = 27/57 (47%), Gaps = 3/57 (5%) Frame = -2 Query: 563 HTNSYSFCFNTCLVHCLETVVEPSIDFSLG---NKKEQFFKNHFSLQRKVSGLNPNS 402 H + F T + CL +EP + ++ G + Q+ + +L + +S +N NS Sbjct: 97 HFSDIPLIFGTLIFTCLSAALEPLMTWTTGKVFDALSQYATSQITLGKMISLINFNS 153 >SPAC1F12.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 377 Score = 25.8 bits (54), Expect = 5.0 Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 5/40 (12%) Frame = +2 Query: 14 LPKSVWKIEDVTKLILNECDRHLATAQ-LIFE----IGNN 118 L K W+IE+ K +N C H T + IF+ +GNN Sbjct: 210 LTKISWRIEEQIKAQINGCSTHTGTKKPYIFKETRLLGNN 249 >SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 678 Score = 25.4 bits (53), Expect = 6.5 Identities = 8/20 (40%), Positives = 14/20 (70%) Frame = -2 Query: 569 TNHTNSYSFCFNTCLVHCLE 510 + H +S+S + TC++H LE Sbjct: 272 SKHVDSFSDVYPTCIIHILE 291 >SPAC26H5.12 |rpo41||mitochondrial DNA-directed RNA polymerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1120 Score = 25.0 bits (52), Expect = 8.7 Identities = 18/81 (22%), Positives = 34/81 (41%) Frame = +1 Query: 307 LLHCQEYGNEIIAFTDNEFKTINNPFGPAKLKLLGFKPETFLCKEKWFLKNCSFLFPNEK 486 L+H + E + ++ N F P++L+LLG T + + + EK Sbjct: 144 LMHESVFSPEEVKLIMDQLNIPINNFTPSQLQLLGITNSTIVGESENGKDQNGDSSLKEK 203 Query: 487 SIEGSTTVSRQCTKHVLKQKL 549 + TTV++ + L+ L Sbjct: 204 QPDVETTVTKSANLNALRSSL 224 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,360,638 Number of Sequences: 5004 Number of extensions: 47182 Number of successful extensions: 134 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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