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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20886
         (405 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_31564| Best HMM Match : ubiquitin (HMM E-Value=0.0026)              32   0.20 
SB_13806| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   1.4  
SB_25949| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   3.3  
SB_17136| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   3.3  
SB_19342| Best HMM Match : Keratin_B2 (HMM E-Value=0.41)               27   5.8  

>SB_31564| Best HMM Match : ubiquitin (HMM E-Value=0.0026)
          Length = 309

 Score = 31.9 bits (69), Expect = 0.20
 Identities = 20/81 (24%), Positives = 42/81 (51%), Gaps = 2/81 (2%)
 Frame = +2

Query: 71  RLGSRSVLASLSRFPARLNSVNYAQAASNAPKITFVKFEPPKEEHHDVR--NAKLNRPLS 244
           RLG  ++  +L   P  +N + +  +A N+P I+  K++ P  + +D    + +L     
Sbjct: 199 RLGHHTINVTLKSIPPHINKLFFTLSAWNSPNIS--KYKNPSLKFYDASYPDKQLCSDSM 256

Query: 245 PHLKYTPSN*LLCSLSHIEQL 307
            H  Y+ +  ++CSL  I+++
Sbjct: 257 EHAAYSQAI-IMCSLCKIDEV 276


>SB_13806| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 116

 Score = 29.1 bits (62), Expect = 1.4
 Identities = 19/67 (28%), Positives = 29/67 (43%), Gaps = 5/67 (7%)
 Frame = +2

Query: 83  RSVLASLSRFPARLNSVNYAQAASNAPKITFVKFEPPKEEHHDVRNAKLNRPLS-----P 247
           R+VL+S       +   +      N     F+ F  PKE+ H V++ KL++  S     P
Sbjct: 45  RTVLSSCCEDVLAIKRTSKIGHLGNKDHTIFINFNFPKEQLHQVKSVKLSQHKSGSQGCP 104

Query: 248 HLKYTPS 268
           H  Y  S
Sbjct: 105 HFAYNVS 111


>SB_25949| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 501

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 10/27 (37%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -2

Query: 299 LCVIESTAVSWKEYI---LNEEKVDDL 228
           +C++ES   SWK+Y+   + +E VD++
Sbjct: 1   MCLVESNRTSWKQYVDRQVADESVDNI 27


>SB_17136| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 974

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = +1

Query: 88  RTSELKQISCEVEQRELRSSRL 153
           +  EL+ + CE+++R+LRSS L
Sbjct: 103 KARELENLRCEIKRRDLRSSEL 124


>SB_19342| Best HMM Match : Keratin_B2 (HMM E-Value=0.41)
          Length = 1093

 Score = 27.1 bits (57), Expect = 5.8
 Identities = 19/66 (28%), Positives = 31/66 (46%)
 Frame = +2

Query: 41   SDNMALFSCTRLGSRSVLASLSRFPARLNSVNYAQAASNAPKITFVKFEPPKEEHHDVRN 220
            SD   + +  + G+R + +S   FP+  N++N  QAAS+ P      F  PK        
Sbjct: 955  SDPPPMNTLKQAGTRQLESSKKGFPSPNNTIN--QAASSYPHSPKYPFPSPKNISKQTAP 1012

Query: 221  AKLNRP 238
            + L+ P
Sbjct: 1013 SCLDSP 1018


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,621,906
Number of Sequences: 59808
Number of extensions: 182757
Number of successful extensions: 396
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 396
length of database: 16,821,457
effective HSP length: 75
effective length of database: 12,335,857
effective search space used: 727815563
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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