SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20857
         (512 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos...    29   0.41 
SPAC22G7.09c |nup45||nucleoporin Nup45|Schizosaccharomyces pombe...    27   1.6  
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|...    26   2.9  
SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyc...    26   2.9  
SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic enzyme|Schizosaccha...    25   5.0  
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p...    25   5.0  
SPCC584.01c |||sulfite reductase NADPH flavoprotein subunit |Sch...    25   5.0  
SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   5.0  
SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pomb...    25   5.0  
SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa...    25   5.0  
SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c...    25   5.0  
SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p...    25   6.7  
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb...    25   8.8  

>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
           Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 29.1 bits (62), Expect = 0.41
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = +2

Query: 200 MYCAESSRGYGGFTSLTVPMEDLSSVTT 283
           M C  +S  +  FTSL +P++D+S V +
Sbjct: 240 MTCGRTSTTFAPFTSLAIPIDDVSHVVS 267


>SPAC22G7.09c |nup45||nucleoporin Nup45|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 16/44 (36%), Positives = 25/44 (56%)
 Frame = -3

Query: 285 EVVTELKSSIGTVSEVKPPYPRDDSAQYMMMPSVTGVAALIFSD 154
           ++  EL++ +GTVSE+    P D +     + SV+  A LI SD
Sbjct: 258 QLAEELQTKLGTVSELVESVPNDVAEVQRRLSSVS-TALLIDSD 300


>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 997

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 21/56 (37%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
 Frame = +1

Query: 271 FRYDLGS-GSTPVVLTSDRPLPANQWIDIQIARLADSVSMKINLVRSFERRLDRPA 435
           FRYD  S     V   S  P  A   IDI+I  + +    K++++ S   RLDRPA
Sbjct: 151 FRYDPSSIRFHDVTEISLSPESAKVGIDIRI--IGNDAGEKLSILSSTLARLDRPA 204


>SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 364

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 16/38 (42%), Positives = 19/38 (50%)
 Frame = -2

Query: 277 NGTQVFHWNGQ*GEAAVSSRRLGAVHDDAVCHWSGCPD 164
           NGT +  WN + GE     RR G  H  AVC  +  PD
Sbjct: 196 NGTLIRIWNSETGEKICEFRR-GYQH-TAVCQLAFSPD 231


>SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic
           enzyme|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 328

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 17/69 (24%), Positives = 31/69 (44%), Gaps = 3/69 (4%)
 Frame = -3

Query: 276 TELKSSIGTVSEVKPPYPRDDSAQYMMMPSVTGVAAL---IFSDIFMERDGGGALIARNA 106
           T +KS +G +S+  P Y  D+S  +  +   T   A+    FSD+    +    ++   +
Sbjct: 27  TVVKSEMGALSKAYPTYSLDESFSFAPIRESTVSRAMTRRYFSDLDKYAESDIVIVGAGS 86

Query: 105 LPVKAGYAI 79
             + A Y I
Sbjct: 87  AGLTAAYYI 95


>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 517

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 19/63 (30%), Positives = 29/63 (46%), Gaps = 1/63 (1%)
 Frame = +3

Query: 279 RPRIRKHSRGAYK*QAIAREPMDRHSDRPPRGFRLHEDQLSTQLR-ETFG*TG*KPPVRD 455
           RPR R+     ++    +RE   R  +RP R  R ++D     LR  + G +   PP R+
Sbjct: 48  RPRRRRRDESDFRRHRESRERSYREDERPRRE-RRYDDYEPRSLRYSSVGRSRSPPPSRE 106

Query: 456 SDV 464
             V
Sbjct: 107 RSV 109


>SPCC584.01c |||sulfite reductase NADPH flavoprotein subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1006

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 9/21 (42%), Positives = 17/21 (80%)
 Frame = -2

Query: 475 SAHRTSESRTGGFQPVYPNVS 413
           SA+RT +SR+  F+P++ N++
Sbjct: 386 SAYRTDKSRSVAFRPLFDNLT 406


>SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = -2

Query: 502 VHNHGGIVDSAHRTSESRTGGFQPVYPNVS 413
           ++ HG +V+S+H T  S T  F+    N S
Sbjct: 119 INTHGTVVNSSHETIASATSLFENSSSNFS 148


>SPCC663.15c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 657

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 15/55 (27%), Positives = 23/55 (41%)
 Frame = +1

Query: 343 WIDIQIARLADSVSMKINLVRSFERRLDRPAESLRFETPMFCGRSXRFHRGCEPQ 507
           W  IQ+++    +   INLV  ++    R    L    P  CG    ++R C  Q
Sbjct: 476 WDVIQLSKAGAPLVKFINLVERYQSCFVRFIHRLVVNAPDLCGEWCDWYRHCLKQ 530


>SPBC25B2.04c |||mitochondrial ribosome assembly
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 328

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 9/22 (40%), Positives = 16/22 (72%)
 Frame = -2

Query: 82  DQLLRLAVLACVRHGTSTPVTV 17
           + +L+L+++ CV+ G   PVTV
Sbjct: 217 EDMLKLSLVGCVKEGIVHPVTV 238


>SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 10/37 (27%), Positives = 20/37 (54%)
 Frame = -2

Query: 493 HGGIVDSAHRTSESRTGGFQPVYPNVSRSCVLS*SSW 383
           H G    AHR ++ +T  F+P++     + +++ S W
Sbjct: 422 HIGTTGMAHRKADVKTFEFRPLHAQRRANVIVTVSGW 458


>SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 473

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 16/57 (28%), Positives = 26/57 (45%)
 Frame = +1

Query: 289 SGSTPVVLTSDRPLPANQWIDIQIARLADSVSMKINLVRSFERRLDRPAESLRFETP 459
           + S PV  T  RP P   W D+ +   + + S+    V + E  +   +E+  F TP
Sbjct: 170 TNSPPVYHTVLRPTPETLWQDLCLLSESFANSLSDEAVLTLESNILLASEAPLFLTP 226


>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 605

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = -3

Query: 252 TVSEVKPPYPRDDSAQYM 199
           T+S VKPP P  DS  ++
Sbjct: 221 TISSVKPPSPNSDSKFFL 238


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,097,272
Number of Sequences: 5004
Number of extensions: 39271
Number of successful extensions: 135
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -