Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20856
(357 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 45 1e-05
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 43 7e-05
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 42 1e-04
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 42 2e-04
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 38 0.002
At1g54860.1 68414.m06263 expressed protein 28 1.6
At5g54920.1 68418.m06840 expressed protein 27 4.9
At1g11480.1 68414.m01319 eukaryotic translation initiation facto... 27 4.9
At5g23600.1 68418.m02769 tRNA 2'phosphotransferase, putative con... 26 6.4
At2g45330.1 68415.m05641 tRNA 2'phosphotransferase, putative con... 26 6.4
At3g47420.1 68416.m05156 glycerol-3-phosphate transporter, putat... 26 8.5
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 45.2 bits (102), Expect = 1e-05
Identities = 21/49 (42%), Positives = 32/49 (65%)
Frame = +2
Query: 209 MENTELXTNTRCSXGLXKFYKGXSYIILRTTSDSRNNLSWDIHYWIGRE 355
+EN + T + S G KF+ G SYI+L+TT+ +L DIHYW+G++
Sbjct: 26 IENFKPVTVPQESHG--KFFTGDSYIVLKTTASRSGSLHHDIHYWLGKD 72
Score = 30.7 bits (66), Expect = 0.30
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 171 AFANVGRTAGVQIWRIQNXEPIPV 242
A G+ +G++IWRI+N +P+ V
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTV 34
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 42.7 bits (96), Expect = 7e-05
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +2
Query: 254 LXKFYKGXSYIILRTTSDSRNNLSWDIHYWIGRE 355
+ KF+ G SYI+L+TT+ L DIHYW+G++
Sbjct: 39 IGKFFTGDSYIVLKTTALKTGALRHDIHYWLGKD 72
Score = 36.7 bits (81), Expect = 0.005
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 168 PAFANVGRTAGVQIWRIQNXEPIPV 242
PAF G+ AG++IWRI+N P P+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPI 34
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 41.9 bits (94), Expect = 1e-04
Identities = 19/49 (38%), Positives = 29/49 (59%)
Frame = +2
Query: 209 MENTELXTNTRCSXGLXKFYKGXSYIILRTTSDSRNNLSWDIHYWIGRE 355
+EN E + G KFY G +YI+L+TT + +DIH+WIG++
Sbjct: 24 IENFEAVPVPKSEHG--KFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKD 70
Score = 35.5 bits (78), Expect = 0.010
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +3
Query: 168 PAFANVGRTAGVQIWRIQNXEPIPV 242
PAF G+ G +IWRI+N E +PV
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPV 32
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 41.5 bits (93), Expect = 2e-04
Identities = 15/32 (46%), Positives = 23/32 (71%)
Frame = +2
Query: 260 KFYKGXSYIILRTTSDSRNNLSWDIHYWIGRE 355
KFY G +YI+L+TT + +DIH+WIG++
Sbjct: 41 KFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKD 72
Score = 40.3 bits (90), Expect = 4e-04
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +3
Query: 168 PAFANVGRTAGVQIWRIQNXEPIPV 242
PAF VG+ G +IWRI+N EP+PV
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPV 34
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/45 (37%), Positives = 27/45 (60%)
Frame = +2
Query: 215 NTELXTNTRCSXGLXKFYKGXSYIILRTTSDSRNNLSWDIHYWIG 349
N +L + + S G KF+ G +Y++LRT + +DIHYW+G
Sbjct: 28 NKQLISIPKSSFG--KFHSGNAYLVLRTFLRKIESPQYDIHYWLG 70
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 217 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 116
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
>At5g54920.1 68418.m06840 expressed protein
Length = 517
Score = 26.6 bits (56), Expect = 4.9
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +3
Query: 81 ARTATKPXEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQI 209
ART T E +G ++ + RD+ K HP+ N R AGV+I
Sbjct: 155 ARTLTS-GETAG---AMKEPGRRDENKYHPSSLNHQRQAGVRI 193
>At1g11480.1 68414.m01319 eukaryotic translation initiation
factor-related contains weak similarity to
Swiss-Prot:P23588 eukaryotic translation initiation
factor 4B (eIF-4B) [Homo sapiens]
Length = 578
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +3
Query: 60 VFLGSVWARTATKPXEISGPITSLSDKNARDKAKVHPAFANVGR 191
VF + W + +P + + P +S + +N DK+ P N+GR
Sbjct: 41 VFDRAPWGNSIGRPDQRARPSSSHAIRNLDDKSLFLPHNVNIGR 84
>At5g23600.1 68418.m02769 tRNA 2'phosphotransferase, putative
contains similarity to Swiss-Prot:Q12272 tRNA
2'phosphotransferase [Saccharomyces cerevisiae];
contains Pfam domain PF01885: RNA 2'-phosphotransferase,
Tpt1 / KptA family
Length = 212
Score = 26.2 bits (55), Expect = 6.4
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = +1
Query: 142 TPVTKPKSIRLLPMSAEQPVCKYGEYRTXNQYPLLXRTXKILQRXFIH 285
T V K ++ + E PVC +G YR N +L K + R +H
Sbjct: 93 TTVESEKLLKPILSPEEAPVCVHGTYR-KNLESILASGLKRMNRMHVH 139
>At2g45330.1 68415.m05641 tRNA 2'phosphotransferase, putative
contains similarity to Swiss-Prot:Q12272 tRNA
2'phosphotransferase [Saccharomyces cerevisiae];
contains Pfam domain PF01885: RNA 2'-phosphotransferase,
Tpt1 / KptA family
Length = 257
Score = 26.2 bits (55), Expect = 6.4
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = +1
Query: 142 TPVTKPKSIRLLPMSAEQPVCKYGEYRTXNQYPLLXRTXKILQRXFIH 285
T V K ++ + E PVC +G YR N +L K + R +H
Sbjct: 138 TTVESEKLLKPILSPEEAPVCVHGTYR-KNLESILASGLKRMNRMHVH 184
>At3g47420.1 68416.m05156 glycerol-3-phosphate transporter, putative
/ glycerol 3-phosphate permease, putative similar to
cAMP inducible 2 protein [Mus musculus] GI:4580997,
glycerol-3-phosphate transporter (glycerol 3-phosphate
permease) [Homo sapiens] GI:7543982; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 523
Score = 25.8 bits (54), Expect = 8.5
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +3
Query: 279 HTSFYGRPRTVATIYHGISTTGSVGS 356
H+S G R +AT+ I TGSVG+
Sbjct: 429 HSSLKGNSRALATVTAIIDGTGSVGA 454
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,796,552
Number of Sequences: 28952
Number of extensions: 140892
Number of successful extensions: 335
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 325
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 335
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 459356736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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