BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20845 (717 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar ... 29 3.1 At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar ... 29 4.1 At2g40860.1 68415.m05044 protein kinase family protein / protein... 28 7.1 >At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar to SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa Transmembrane trafficking protein) {Homo sapiens}; contains Pfam profile PF01105: emp24/gp25L/p24 family Length = 212 Score = 29.1 bits (62), Expect = 3.1 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = +1 Query: 310 LKSAAHILHSFRIFLTRTLEQDKR*VNVPTVCGVSWYSLRIASRCVPIFAGQIAH 474 L+ A +H I+L R E + R V+ T V+WYS+ C+ + QI + Sbjct: 148 LEGAVEAIHENLIYL-RNREAEMRIVSEKTNSRVAWYSIMSLGICIVVSGLQILY 201 >At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar to SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa Transmembrane trafficking protein) {Homo sapiens}; contains Pfam profile PF01105: emp24/gp25L/p24 family Length = 217 Score = 28.7 bits (61), Expect = 4.1 Identities = 15/55 (27%), Positives = 27/55 (49%) Frame = +1 Query: 310 LKSAAHILHSFRIFLTRTLEQDKR*VNVPTVCGVSWYSLRIASRCVPIFAGQIAH 474 L+ A +H ++L R E D R ++ T V+WYS+ C+ + Q+ + Sbjct: 153 LEGAVEAIHENILYL-RNREADMRTMSEKTNSRVAWYSIMSLGVCIAVSGFQVLY 206 >At2g40860.1 68415.m05044 protein kinase family protein / protein phosphatase 2C ( PP2C) family protein contains Pfam PF00481: Protein phosphatase 2C domain; contains Pfam PF00069: Protein kinase domain; similar to partner of PIX 1 (GI:21702695) [Homo sapiens] Length = 658 Score = 27.9 bits (59), Expect = 7.1 Identities = 13/46 (28%), Positives = 24/46 (52%) Frame = -3 Query: 505 PFTAYIAQAHDVRSVRQISARTWRQSGANTN*PHKLSAR*LIVYPA 368 P+ A A +++R+++ + WR SG T HK + ++Y A Sbjct: 163 PYLADFGLAEYKKNLREVNLQNWRSSGKPTGGFHKKNMVGTLIYMA 208 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,256,107 Number of Sequences: 28952 Number of extensions: 277329 Number of successful extensions: 511 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 503 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 511 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1555552968 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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