Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20837
(338 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 49 9e-07
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 49 9e-07
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 49 9e-07
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 49 9e-07
At4g17160.1 68417.m02582 Ras-related GTP-binding protein, putati... 29 1.0
At2g46380.1 68415.m05773 hypothetical protein weak similarity to... 27 2.4
At4g16563.1 68417.m02506 aspartyl protease family protein contai... 27 3.2
At1g78230.1 68414.m09116 leucine-rich repeat family protein 27 3.2
At4g10720.1 68417.m01752 ankyrin repeat family protein contains ... 27 4.2
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 27 4.2
At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive eff... 25 9.7
At4g21890.1 68417.m03165 expressed protein 25 9.7
At4g11110.1 68417.m01803 WD-40 repeat family protein / phytochro... 25 9.7
At4g02110.1 68417.m00282 BRCT domain-containing protein contains... 25 9.7
At2g27420.1 68415.m03314 cysteine proteinase, putative contains ... 25 9.7
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 48.8 bits (111), Expect = 9e-07
Identities = 34/76 (44%), Positives = 39/76 (51%), Gaps = 11/76 (14%)
Frame = +2
Query: 38 FEDPEFPAVDRSLYYK-----ESLDRPITWLRPGEI-----SDN-PQLFVEGYSRFDVQQ 184
F D EFP DRSL+ L W+RP I SD+ P LF + DV Q
Sbjct: 1696 FTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQ 1755
Query: 185 GELGDCWLLAAVANLT 232
G LGDCW L+AVA LT
Sbjct: 1756 GRLGDCWFLSAVAVLT 1771
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 48.8 bits (111), Expect = 9e-07
Identities = 34/76 (44%), Positives = 39/76 (51%), Gaps = 11/76 (14%)
Frame = +2
Query: 38 FEDPEFPAVDRSLYYK-----ESLDRPITWLRPGEI-----SDN-PQLFVEGYSRFDVQQ 184
F D EFP DRSL+ L W+RP I SD+ P LF + DV Q
Sbjct: 1696 FTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQ 1755
Query: 185 GELGDCWLLAAVANLT 232
G LGDCW L+AVA LT
Sbjct: 1756 GRLGDCWFLSAVAVLT 1771
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 48.8 bits (111), Expect = 9e-07
Identities = 34/76 (44%), Positives = 39/76 (51%), Gaps = 11/76 (14%)
Frame = +2
Query: 38 FEDPEFPAVDRSLYYK-----ESLDRPITWLRPGEI-----SDN-PQLFVEGYSRFDVQQ 184
F D EFP DRSL+ L W+RP I SD+ P LF + DV Q
Sbjct: 1696 FTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQ 1755
Query: 185 GELGDCWLLAAVANLT 232
G LGDCW L+AVA LT
Sbjct: 1756 GRLGDCWFLSAVAVLT 1771
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 48.8 bits (111), Expect = 9e-07
Identities = 34/76 (44%), Positives = 39/76 (51%), Gaps = 11/76 (14%)
Frame = +2
Query: 38 FEDPEFPAVDRSLYYK-----ESLDRPITWLRPGEI-----SDN-PQLFVEGYSRFDVQQ 184
F D EFP DRSL+ L W+RP I SD+ P LF + DV Q
Sbjct: 1696 FTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQ 1755
Query: 185 GELGDCWLLAAVANLT 232
G LGDCW L+AVA LT
Sbjct: 1756 GRLGDCWFLSAVAVLT 1771
>At4g17160.1 68417.m02582 Ras-related GTP-binding protein, putative
similar to GTP-binding protein GI:1208537 from [Glycine
max]
Length = 205
Score = 28.7 bits (61), Expect = 1.0
Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%)
Frame = -1
Query: 257 TGRITCDEVSSW---QRQPTANNRPTLLVGRRSDCSLRRTV 144
T R T + ++SW RQ + N T+L+G + D +RTV
Sbjct: 89 TRRETFNHLASWLEEARQHASENMTTMLIGNKCDLEDKRTV 129
>At2g46380.1 68415.m05773 hypothetical protein weak similarity to
extra-large G-protein [Arabidopsis thaliana] GI:3201682
Length = 732
Score = 27.5 bits (58), Expect = 2.4
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 44 DPEFPAVDRSLYYKESLDRPITWLRPGEISDNPQL 148
+P F +RS + SL P T++ PG S PQL
Sbjct: 291 NPGFYPHERSFGFGTSLHSPRTFIPPGSQSPGPQL 325
>At4g16563.1 68417.m02506 aspartyl protease family protein contains
Pfam profile: PF00026 eukaryotic aspartyl protease
Length = 499
Score = 27.1 bits (57), Expect = 3.2
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 102 PSLGSDLGKSATTLNCSSKATVASTSNKES 191
PS S L SATT++CSS + A+ S+ S
Sbjct: 126 PSPPSSLSSSATTVSCSSPSCSAAHSSLPS 155
>At1g78230.1 68414.m09116 leucine-rich repeat family protein
Length = 676
Score = 27.1 bits (57), Expect = 3.2
Identities = 20/49 (40%), Positives = 23/49 (46%), Gaps = 5/49 (10%)
Frame = -2
Query: 181 LDVEATVAFDEQLRVVADFPRS-----EPSDGPVQALLVVEAAVHGRKL 50
LDVE V +E V+A FP S P VQ+ V EA VH L
Sbjct: 370 LDVETVVPVNEDKDVLAIFPTSPNTERSPLGNVVQSGNVSEAIVHANSL 418
>At4g10720.1 68417.m01752 ankyrin repeat family protein contains
Pfam domain, PF00023: Ankyrin repeat
Length = 445
Score = 26.6 bits (56), Expect = 4.2
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = -2
Query: 286 LHXNSDHQVQLE-E*LAMKCQVGNGSQQPTIAQLSLLDVEATVAFDEQLRVVADFPRSEP 110
LH DH E + K +G+ P ++S + + + ++F E L R++
Sbjct: 226 LHNQRDHHANSNIENIIRKWGGKSGNSLPKSKKVSEI-LRSPISFTEHLFTQTARYRNQT 284
Query: 109 SDGPVQALLVVEAAV 65
S+G ALLV+ A +
Sbjct: 285 SEGTRSALLVIAALI 299
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 26.6 bits (56), Expect = 4.2
Identities = 14/48 (29%), Positives = 26/48 (54%)
Frame = +2
Query: 29 GRLFEDPEFPAVDRSLYYKESLDRPITWLRPGEISDNPQLFVEGYSRF 172
G+L D + +DR LY+ ++ +PI ++R G +D + G S +
Sbjct: 560 GKLSSDSDHEVLDRMLYFLDTSCQPI-YIREGNETDTFWNLLGGKSEY 606
>At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1311
Score = 25.4 bits (53), Expect = 9.7
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = +2
Query: 2 DIRSRCLAEGRLFEDPEFPAVDRSLYYKESLDRP 103
D SR +GRL + +YYK+S+ +P
Sbjct: 1018 DFPSRYFYQGRLTDSESVSTAPDEIYYKDSVLKP 1051
>At4g21890.1 68417.m03165 expressed protein
Length = 266
Score = 25.4 bits (53), Expect = 9.7
Identities = 12/42 (28%), Positives = 24/42 (57%)
Frame = +2
Query: 194 GDCWLLAAVANLTLHRKLFFQLYLMIRVSMKIMRASSISVSG 319
GD W +A N+ + R + LM+ + +++++ S+ VSG
Sbjct: 113 GDSWEMARDPNVEVFRVTYELESLMLPSNKRMVKSLSLEVSG 154
>At4g11110.1 68417.m01803 WD-40 repeat family protein / phytochrome
A-related contains 7 WD-40 repeats (PF00400); similar to
phytochrome A supressor spa1 (GI:4809171) [Arabidopsis
thaliana]; contains non-consensus (GC) donor splice
sites at introns 4 and 6
Length = 1017
Score = 25.4 bits (53), Expect = 9.7
Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
Frame = +2
Query: 2 DIRSRCLAEGRLFEDPEFPAVDRSLYYKESLDRPIT--WLRPGEISDNPQLFVEGYS 166
DIR R L L E+P+ L + ES RP T L+ ++ P L+ EG S
Sbjct: 487 DIRHRILPPKFLSENPKEAGFCLWLLHPESSCRPSTRDILQSEVVNGIPDLYAEGLS 543
>At4g02110.1 68417.m00282 BRCT domain-containing protein contains
Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain
Length = 1293
Score = 25.4 bits (53), Expect = 9.7
Identities = 19/71 (26%), Positives = 32/71 (45%)
Frame = -2
Query: 244 LAMKCQVGNGSQQPTIAQLSLLDVEATVAFDEQLRVVADFPRSEPSDGPVQALLVVEAAV 65
L + V Q + + +VE TV + +L D P +PSDG +Q+ + +
Sbjct: 676 LVLDLMVNQDKLQAKTPEAADAEVEITV-LERELN---DVPTEDPSDGALQSEVDKNTSK 731
Query: 64 HGRKLGVFEES 32
R+ GV + S
Sbjct: 732 RKREAGVGKNS 742
>At2g27420.1 68415.m03314 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 348
Score = 25.4 bits (53), Expect = 9.7
Identities = 23/71 (32%), Positives = 28/71 (39%), Gaps = 11/71 (15%)
Frame = +2
Query: 44 DPEFPAVDRSLYYKESLDRPITW--------LRPGEISDNPQLF---VEGYSRFDVQQGE 190
D EF A L E++ R T R G +SDN + EG QG
Sbjct: 89 DEEFRATHTGLVVPEAITRISTLSSGKNTVPFRYGNVSDNGESMDWRQEGAVTPVKYQGR 148
Query: 191 LGDCWLLAAVA 223
G CW +AVA
Sbjct: 149 CGGCWAFSAVA 159
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,494,489
Number of Sequences: 28952
Number of extensions: 139104
Number of successful extensions: 427
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 416
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 422
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 399440640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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