Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20836
(513 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 85 8e-18
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 37 0.002
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 36 0.004
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 34 0.011
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 26 3.8
SPCC1393.08 |||transcription factor, zf-GATA type |Schizosacchar... 26 3.8
SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces ... 25 5.0
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 25 6.7
SPAC1250.04c |atl1||alkyltransferase-like protein Atl1|Schizosac... 25 6.7
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 8.8
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 25 8.8
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 84.6 bits (200), Expect = 8e-18
Identities = 35/66 (53%), Positives = 47/66 (71%)
Frame = +1
Query: 256 NYMDEADVRSVDENFDVHDIPMDSIWLDIEYTNKKMYFTWDVVKFPHPAEMVANLTAKGR 435
NY+ E DV +VD FD D+P D+IWLDIEY +K+ YFTWD FP+P M+ L +K R
Sbjct: 384 NYVSEEDVLNVDAKFDEVDMPYDTIWLDIEYASKRRYFTWDKATFPNPKAMLEKLDSKSR 443
Query: 436 KMVVIV 453
K++VI+
Sbjct: 444 KLIVIL 449
Score = 60.9 bits (141), Expect = 1e-10
Identities = 31/83 (37%), Positives = 42/83 (50%)
Frame = +2
Query: 2 WHNSAETWIDVVNYGEGNVVSSLVNLVTGGQKKRVDARFLSESGIVDMFVLMGSTPGDAF 181
W N+A TWIDV + S + + SESG +D+F+ +G D +
Sbjct: 310 WSNAAATWIDVEKESGPSPHS-----------QSTSTHWYSESGTLDLFIFLGPKASDVY 358
Query: 182 RQYTALTGTTPLPPKFSLGYHQC 250
Y+AL G LPP FS+GYHQC
Sbjct: 359 ESYSALVGRPLLPPLFSIGYHQC 381
Score = 26.6 bits (56), Expect = 2.2
Identities = 8/14 (57%), Positives = 12/14 (85%)
Frame = +2
Query: 455 DPHIKREPGYFLTR 496
DPHIK +P YF+++
Sbjct: 450 DPHIKNDPNYFVSK 463
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 36.7 bits (81), Expect = 0.002
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 2/70 (2%)
Frame = +1
Query: 259 YMDEADVRSVDENFDVHDIPMDSIWLDIEYTNKKMYFTWDVVKFPHP--AEMVANLTAKG 432
Y + D+ V ENF +IP+D+ W DI+Y + FT + FP E +L
Sbjct: 345 YKNVFDLVEVKENFKNFEIPVDTFWSDIDYMYEYRDFTVESNAFPKDKMMEFFNSLQQSN 404
Query: 433 RKMVVIVGSA 462
+ V I+ +A
Sbjct: 405 QHYVPIIDAA 414
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 35.9 bits (79), Expect = 0.004
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +1
Query: 259 YMDEADVRSVDENFDVHDIPMDSIWLDIEYTNKKMYFTWDVVKF 390
Y + ++ V +N+ DIP+++ W DI+Y K FT D V +
Sbjct: 329 YTNITEIMDVRQNYIDADIPVETFWSDIDYMEKYRDFTVDPVSY 372
Score = 35.5 bits (78), Expect = 0.005
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +2
Query: 131 GIVDMFVLMGST--PGDAFRQYTALTGTTPLPPKFSLGYHQC 250
G++D+FV GST P + +Q+ G + ++LGYH C
Sbjct: 284 GVIDLFVYSGSTESPKETVKQFVQSIGKPAMHQYWTLGYHSC 325
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 34.3 bits (75), Expect = 0.011
Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +2
Query: 131 GIVDMFVLMGST--PGDAFRQYTALTGTTPLPPKFSLGY 241
GI+D+FV GST P +QY GT + P +SLG+
Sbjct: 302 GIIDLFVYSGSTVSPKYTIQQYVQSIGTPTMQPYWSLGF 340
Score = 27.9 bits (59), Expect = 0.94
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +1
Query: 259 YMDEADVRSVDENFDVHDIPMDSIWLDIEYTNKKMYFTWDVVKFP 393
Y +D+ ++ + +IP + W DI+Y ++ FT + FP
Sbjct: 347 YKTLSDLINMRSYLNASNIPTEGFWNDIDYMSEFRTFTVNSTAFP 391
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 25.8 bits (54), Expect = 3.8
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = -2
Query: 479 QVRV*CADPTMTTIFLPLAVRLATIS 402
++R+ C DP+ T+F PL V ++ +S
Sbjct: 1462 EIRIICTDPSTNTLF-PLRVIISNVS 1486
>SPCC1393.08 |||transcription factor, zf-GATA type
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 25.8 bits (54), Expect = 3.8
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +1
Query: 25 DRCGQLRGRERGLVARQPGHRGTEETRRCKVPERVRHRGHVRADGL 162
DR GQ GL AR GH ++ K+ R R +G DG+
Sbjct: 433 DRHGQTVCNACGLYARLHGHNRPIGLKKNKITRRRRGKGPGGEDGM 478
>SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 886
Score = 25.4 bits (53), Expect = 5.0
Identities = 17/59 (28%), Positives = 22/59 (37%), Gaps = 3/59 (5%)
Frame = -1
Query: 510 NQSRPRVRKYPGSRLMCGSHDDNHLPSFGRQV---GHHFRRMRELDHVPREVHLLVGVL 343
N S+PR PG M S D + PS V +F + R P + V L
Sbjct: 254 NSSKPRTSLQPGESPMKSSRDISRKPSMASSVLSPSDYFPQHRRFQSAPAPIPRPVSTL 312
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.0 bits (52), Expect = 6.7
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 298 FDVHDIPMDSIWLDIEYTNKKMYFTWDV 381
FD++DI +DSI Y N+ + + V
Sbjct: 288 FDIYDIILDSIQESFSYVNRFLILDFSV 315
>SPAC1250.04c |atl1||alkyltransferase-like protein
Atl1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 108
Score = 25.0 bits (52), Expect = 6.7
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = -1
Query: 438 LPSFGRQVGHHFRRMRELDHVP 373
+PS+ RQVG + + HVP
Sbjct: 34 MPSYARQVGQAMKHLHPETHVP 55
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 24.6 bits (51), Expect = 8.8
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = +1
Query: 223 KILAGLPPVPLNYMDEADVRSVDENFDVHDIP-MDSIWLDIEYTN 354
++L G +PL ++ E R EN D IP ++S+ L I N
Sbjct: 200 EVLIGAEGIPLEFLSEFVQRFEHENLDELFIPVLESLSLKIGLMN 244
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 24.6 bits (51), Expect = 8.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +3
Query: 453 GIRTSNANLDIFLHEDATD 509
G R S AN+ ++ H D TD
Sbjct: 288 GARLSGANIRVYKHNDMTD 306
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,198,655
Number of Sequences: 5004
Number of extensions: 44206
Number of successful extensions: 143
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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