BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20818 (710 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_19354| Best HMM Match : MPPN (HMM E-Value=2.9) 36 0.043 SB_56787| Best HMM Match : Keratin_B2 (HMM E-Value=0.34) 28 8.6 >SB_19354| Best HMM Match : MPPN (HMM E-Value=2.9) Length = 290 Score = 35.5 bits (78), Expect = 0.043 Identities = 18/48 (37%), Positives = 28/48 (58%) Frame = -2 Query: 649 MFHFR*STIVLYFPKNNALF*NCFFTLSNRSAVLRPDYNNENYILLAR 506 +FHF + I+ YF KNNAL NC + L ++ Y ++Y+LL + Sbjct: 88 IFHFLKTFIIKYFLKNNALI-NCCYKLYIFNSFQAIQYQEQSYLLLVK 134 >SB_56787| Best HMM Match : Keratin_B2 (HMM E-Value=0.34) Length = 527 Score = 27.9 bits (59), Expect = 8.6 Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 3/95 (3%) Frame = +1 Query: 394 NFVCITIIASYSKI-QILNNSVDDTNRNFMSFSKCQSFISLTKCNFHYCSQVLTPLTCSI 570 N + +T +++ + + LN+ T N ++ + C I +T C++H +T TC Sbjct: 420 NHITVTTCHNHTTVTKCLNHITVTTCHNHITVTTCHDHIPVTTCHYH-----ITVTTC-- 472 Query: 571 K*RNNFKTVHCSSE-SITQWLT-IESETCHWYXPL 669 N+ C + ++T I TCH + P+ Sbjct: 473 --HNHITVTKCLNHITVTTCHNHITVTTCHDHIPV 505 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,550,490 Number of Sequences: 59808 Number of extensions: 341241 Number of successful extensions: 639 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 599 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 637 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1877743452 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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