BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20332 (579 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g33010.1 68417.m04695 glycine dehydrogenase [decarboxylating]... 125 2e-29 At2g26080.1 68415.m03131 glycine dehydrogenase [decarboxylating]... 119 1e-27 At1g30130.2 68414.m03683 expressed protein similar to hypothetic... 33 0.18 At1g30130.1 68414.m03682 expressed protein similar to hypothetic... 33 0.18 At2g06775.1 68415.m00756 hypothetical protein 28 3.9 At5g59920.1 68418.m07514 DC1 domain-containing protein contains ... 27 6.8 At5g51200.1 68418.m06349 expressed protein 27 6.8 At5g34850.1 68418.m04090 calcineurin-like phosphoesterase family... 27 9.0 At3g16500.1 68416.m02106 auxin-responsive AUX/IAA family protein... 27 9.0 At3g13450.1 68416.m01692 2-oxoisovalerate dehydrogenase / 3-meth... 27 9.0 At1g55510.1 68414.m06350 2-oxoisovalerate dehydrogenase, putativ... 27 9.0 >At4g33010.1 68417.m04695 glycine dehydrogenase [decarboxylating], putative / glycine decarboxylase, putative / glycine cleavage system P-protein, putative strong similarity to SP|P49361 Glycine dehydrogenase [decarboxylating] A, mitochondrial precursor (EC 1.4.4.2) {Flaveria pringlei}; contains Pfam profile PF02347: Glycine cleavage system P-protein Length = 1037 Score = 125 bits (302), Expect = 2e-29 Identities = 54/85 (63%), Positives = 64/85 (75%) Frame = +1 Query: 1 PCSYKHFTDIHPFAPLEQCQGYHTLFEELANDLCAITGYDRVSFQPNSGAQGEYAGLRTI 180 P ++ FTDIHPFAP+EQ QGY +FE L + LC ITG+D S QPN+GA GEYAGL I Sbjct: 590 PVTWPSFTDIHPFAPVEQAQGYQEMFENLGDLLCTITGFDSFSLQPNAGAAGEYAGLMVI 649 Query: 181 KRYHEYRGDAGRNICLIPVSAHGTN 255 + YH RGD RN+C+IPVSAHGTN Sbjct: 650 RAYHMSRGDHHRNVCIIPVSAHGTN 674 Score = 108 bits (260), Expect = 2e-24 Identities = 47/95 (49%), Positives = 67/95 (70%) Frame = +3 Query: 255 PASAHMAGMRVCAIRVTPTGDIDMAHLKDMVEEHSEKVSCLMLTYPSTFGVFEEKAADIC 434 PASA M GM++ + G+I++ ++ E + + ++ LM+TYPST GV+EE +IC Sbjct: 675 PASAAMCGMKIITVGTDAKGNINIEEVRKAAEANKDNLAALMVTYPSTHGVYEEGIDEIC 734 Query: 435 ALVHAHGGQVYLDGANMNAQVGLCSPGDYGSDVSH 539 ++H +GGQVY+DGANMNAQVGL SPG G+DV H Sbjct: 735 NIIHENGGQVYMDGANMNAQVGLTSPGFIGADVCH 769 >At2g26080.1 68415.m03131 glycine dehydrogenase [decarboxylating], putative / glycine decarboxylase, putative / glycine cleavage system P-protein, putative strong similarity to SP|P26969 Glycine dehydrogenase [decarboxylating], mitochondrial precursor (EC 1.4.4.2) {Pisum sativum}; contains Pfam profile PF02347: Glycine cleavage system P-protein Length = 1044 Score = 119 bits (287), Expect = 1e-27 Identities = 51/85 (60%), Positives = 62/85 (72%) Frame = +1 Query: 1 PCSYKHFTDIHPFAPLEQCQGYHTLFEELANDLCAITGYDRVSFQPNSGAQGEYAGLRTI 180 P ++ FT++HPFAP+EQ QGY +F L LC ITG+D S QPN+GA GEYAGL I Sbjct: 596 PVTWPSFTNMHPFAPVEQAQGYQEMFTNLGELLCTITGFDSFSLQPNAGAAGEYAGLMVI 655 Query: 181 KRYHEYRGDAGRNICLIPVSAHGTN 255 + YH RGD RN+C+IPVSAHGTN Sbjct: 656 RAYHMSRGDHHRNVCIIPVSAHGTN 680 Score = 112 bits (269), Expect = 2e-25 Identities = 49/95 (51%), Positives = 69/95 (72%) Frame = +3 Query: 255 PASAHMAGMRVCAIRVTPTGDIDMAHLKDMVEEHSEKVSCLMLTYPSTFGVFEEKAADIC 434 PASA M GM++ A+ G+I++ L++ E + + ++ LM+TYPST GV+EE +IC Sbjct: 681 PASAAMCGMKIVAVGTDAKGNINIEELRNAAEANKDNLAALMVTYPSTHGVYEEGIDEIC 740 Query: 435 ALVHAHGGQVYLDGANMNAQVGLCSPGDYGSDVSH 539 ++H +GGQVY+DGANMNAQVGL SPG G+DV H Sbjct: 741 NIIHENGGQVYMDGANMNAQVGLTSPGFIGADVCH 775 >At1g30130.2 68414.m03683 expressed protein similar to hypothetical protein GI:1469227 from [Brassica oleracea] Length = 311 Score = 32.7 bits (71), Expect = 0.18 Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = -1 Query: 162 IFALGPTIGLERHPI--VSCYSTKIISKFLEQCMISLTLLQWSERM 31 + + P++G E++P+ CY + SK L +C+ +T W ER+ Sbjct: 102 LLTIPPSVGYEQNPLSLYYCYDLEGSSKRLSKCIAQVTNTPWGERV 147 >At1g30130.1 68414.m03682 expressed protein similar to hypothetical protein GI:1469227 from [Brassica oleracea] Length = 311 Score = 32.7 bits (71), Expect = 0.18 Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = -1 Query: 162 IFALGPTIGLERHPI--VSCYSTKIISKFLEQCMISLTLLQWSERM 31 + + P++G E++P+ CY + SK L +C+ +T W ER+ Sbjct: 102 LLTIPPSVGYEQNPLSLYYCYDLEGSSKRLSKCIAQVTNTPWGERV 147 >At2g06775.1 68415.m00756 hypothetical protein Length = 128 Score = 28.3 bits (60), Expect = 3.9 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%) Frame = +3 Query: 324 MAHLKDMVEEHSEKVSCLMLTYPSTFGVFEEKAADIC-ALVHAHGGQVYLDGANMNAQ 494 + HL+D V+ H E V + FEEK D+ L + G V ++ A +N Q Sbjct: 28 IGHLQDFVDGHQEDVKRIGSKMSEMKAKFEEKFDDLTNRLENMEGALVEINNALLNHQ 85 >At5g59920.1 68418.m07514 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 710 Score = 27.5 bits (58), Expect = 6.8 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -1 Query: 558 VFMKVEDETRHCRNLQENINRPAHSC 481 VF+K+ +E C +E+I P++SC Sbjct: 127 VFLKIREEKVPCELCKESIEGPSYSC 152 >At5g51200.1 68418.m06349 expressed protein Length = 1808 Score = 27.5 bits (58), Expect = 6.8 Identities = 14/34 (41%), Positives = 17/34 (50%) Frame = -1 Query: 516 LQENINRPAHSCWHRPNRLDLRVRAPGHIYPPLF 415 L +NINRPA S H + DL G + P F Sbjct: 870 LVDNINRPAPSITHLLLKFDLDAPVEGTVLQPKF 903 >At5g34850.1 68418.m04090 calcineurin-like phosphoesterase family protein contains Pfam profile: PF00149 calcineurin-like phosphoesterase Length = 475 Score = 27.1 bits (57), Expect = 9.0 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = -2 Query: 194 SW*RFIVRNPAYSPW 150 SW RF+ R+ AY PW Sbjct: 207 SWGRFVERSTAYQPW 221 >At3g16500.1 68416.m02106 auxin-responsive AUX/IAA family protein similar to SP|O24408|AXII_ARATH Auxin-responsive protein IAA18 (Indoleacetic acid-induced protein 18) {Arabidopsis thaliana}; contains Pfam profile: PF02309: AUX/IAA family Length = 269 Score = 27.1 bits (57), Expect = 9.0 Identities = 21/74 (28%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Frame = -1 Query: 570 EIQNVFMKVEDETRHCRNLQENINRPAHSCWHRPNRLDLRVRAPGHI--YPPL-FLQRRR 400 EI+N+ + ED++ HC N N P++ P+ + + APG + +PP+ ++ Sbjct: 61 EIRNIKKETEDKSFHCFN--GNHFSPSNKTTSVPH-ISQKRTAPGPVVGWPPVRSFRKNL 117 Query: 399 KYLGKSTLGTKLSH 358 S LG + SH Sbjct: 118 ASTSSSKLGNESSH 131 >At3g13450.1 68416.m01692 2-oxoisovalerate dehydrogenase / 3-methyl-2-oxobutanoate dehydrogenase / branched-chain alpha-keto acid dehydrogenase E1 beta subunit (DIN4) identical to branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] GI:7021286 Length = 358 Score = 27.1 bits (57), Expect = 9.0 Identities = 16/38 (42%), Positives = 19/38 (50%), Gaps = 2/38 (5%) Frame = -2 Query: 305 RDPYGADAHAGHV--RGGRLVPCALTGIKQMFRPASPR 198 R PYGA H GH + C + GIK + P SPR Sbjct: 150 RAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVI-PRSPR 186 >At1g55510.1 68414.m06350 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 beta subunit, putative strong similarity to branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] GI:7021286; contains Pfam profiles PF02779: Transketolase, pyridine binding domain, PF02780: Transketolase, C-terminal domain Length = 352 Score = 27.1 bits (57), Expect = 9.0 Identities = 16/38 (42%), Positives = 19/38 (50%), Gaps = 2/38 (5%) Frame = -2 Query: 305 RDPYGADAHAGHV--RGGRLVPCALTGIKQMFRPASPR 198 R PYGA H GH + C + GIK + P SPR Sbjct: 144 RAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVI-PRSPR 180 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,061,374 Number of Sequences: 28952 Number of extensions: 320515 Number of successful extensions: 873 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 846 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 873 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1131744440 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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