BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20329 (410 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g03310.1 68418.m00282 auxin-responsive family protein similar... 31 0.40 At1g17540.1 68414.m02157 protein kinase-related similar to serin... 29 0.92 At5g49280.1 68418.m06099 hydroxyproline-rich glycoprotein family... 28 2.1 At5g38560.1 68418.m04662 protein kinase family protein contains ... 28 2.8 At3g01560.1 68416.m00086 proline-rich family protein contains pr... 28 2.8 At1g04930.1 68414.m00490 hydroxyproline-rich glycoprotein family... 28 2.8 At3g08670.1 68416.m01007 expressed protein 27 3.7 At2g02480.1 68415.m00187 DNA polymerase-related weak similarity ... 27 3.7 At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 27 4.9 At4g23720.1 68417.m03413 expressed protein ; expression supporte... 27 4.9 At3g52115.1 68416.m05720 hypothetical protein 27 4.9 At3g44340.1 68416.m04764 sec23/sec24 transport family protein co... 27 4.9 At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g... 27 4.9 At4g11530.1 68417.m01850 protein kinase family protein contains ... 27 6.5 At2g11090.1 68415.m01187 expressed protein 27 6.5 At1g79800.1 68414.m09316 plastocyanin-like domain-containing pro... 27 6.5 At1g55940.1 68414.m06416 cytochrome P450, putative similar to SP... 27 6.5 At1g19640.1 68414.m02448 S-adenosyl-L-methionine:jasmonic acid c... 27 6.5 At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase fam... 26 8.6 At3g56590.1 68416.m06293 hydroxyproline-rich glycoprotein family... 26 8.6 At3g48860.2 68416.m05337 expressed protein 26 8.6 At3g48860.1 68416.m05336 expressed protein 26 8.6 At2g16630.1 68415.m01909 proline-rich family protein contains pr... 26 8.6 >At5g03310.1 68418.m00282 auxin-responsive family protein similar to indole-3-acetic acid induced protein ARG7 (SP:P32295) [Vigna radiata] Length = 114 Score = 30.7 bits (66), Expect = 0.40 Identities = 17/49 (34%), Positives = 24/49 (48%) Frame = +3 Query: 168 FRGLLGDGNNEPYDDFRLPNGKICTSESESVTRTVWPAAVHRCKPPEHS 314 FR LL +E YDDF + K+C + E++ V RC P +S Sbjct: 70 FRALLDQSKDEAYDDFTSGDSKLCIACDETLF-----LEVLRCASPRYS 113 >At1g17540.1 68414.m02157 protein kinase-related similar to serine/threonine protein kinase Fen [Lycopersicon esculentum] GI:1809259 Length = 733 Score = 29.5 bits (63), Expect = 0.92 Identities = 20/55 (36%), Positives = 23/55 (41%), Gaps = 6/55 (10%) Frame = +1 Query: 244 RKASR*RVPSGPQLSTGASRPNTP--ITSCTMHPS----PPPANRXSGEYRRSGP 390 R ASR + P GP G P P + HPS P P + S E RS P Sbjct: 155 RSASRPQTPQGPHTPQGPQTPQAPQHPPHPSKHPSMMSDPGPTSSTSSESGRSSP 209 >At5g49280.1 68418.m06099 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 162 Score = 28.3 bits (60), Expect = 2.1 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = +1 Query: 304 PNTPITSCTMHPSPPPANRXSGEY 375 P+TP T+C PSPP + S Y Sbjct: 58 PSTPTTACPPPPSPPSSGGGSSYY 81 >At5g38560.1 68418.m04662 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 681 Score = 27.9 bits (59), Expect = 2.8 Identities = 13/33 (39%), Positives = 15/33 (45%) Frame = +1 Query: 268 PSGPQLSTGASRPNTPITSCTMHPSPPPANRXS 366 P G S P+TP + T P PPPA S Sbjct: 148 PPGETPSPPKPSPSTPTPTTTTSPPPPPATSAS 180 >At3g01560.1 68416.m00086 proline-rich family protein contains proline rich extensin domains, INTERPRO:IPR002965 Length = 511 Score = 27.9 bits (59), Expect = 2.8 Identities = 12/27 (44%), Positives = 13/27 (48%) Frame = +1 Query: 277 PQLSTGASRPNTPITSCTMHPSPPPAN 357 PQL T S P HP PPP+N Sbjct: 273 PQLPTQFSSQQEPYCPPPSHPQPPPSN 299 >At1g04930.1 68414.m00490 hydroxyproline-rich glycoprotein family protein Common family member: At2g32840 [Arabidopsis thaliana] Length = 332 Score = 27.9 bits (59), Expect = 2.8 Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = +1 Query: 277 PQLSTGASRPNTPITSCTMHPS-PPPA 354 P LST + P TP+ + PS PPPA Sbjct: 16 PSLSTASETPVTPVNTVRPPPSQPPPA 42 >At3g08670.1 68416.m01007 expressed protein Length = 567 Score = 27.5 bits (58), Expect = 3.7 Identities = 15/45 (33%), Positives = 22/45 (48%) Frame = +1 Query: 181 SETVTTSLTMTSGYLMERSALRKASR*RVPSGPQLSTGASRPNTP 315 S + TS S Y+ S ++S PS P ++ ASR +TP Sbjct: 177 SSILNTSSASVSSYIRPSSPSSRSSSSARPSTPTRTSSASRSSTP 221 >At2g02480.1 68415.m00187 DNA polymerase-related weak similarity to DNA polymerase III holoenzyme tau subunit [Thermus thermophilus] GI:2583049 Length = 1218 Score = 27.5 bits (58), Expect = 3.7 Identities = 17/72 (23%), Positives = 31/72 (43%) Frame = +3 Query: 24 SRSSKRMCSLSVNQATESVLAHWTASWPSAHLNLRCATLKLTASTWVNFRGLLGDGNNEP 203 SRSS R+ + + S + T + ++ N+ T +W + DG++E Sbjct: 236 SRSSSRLLRANNRKEDSSCTYNSTPALSTSSYNMYAVRNPSTVGSWDGTTTSVNDGDDEL 295 Query: 204 YDDFRLPNGKIC 239 D+ LP + C Sbjct: 296 DDNLDLPGRQGC 307 >At5g15630.1 68418.m01829 phytochelatin synthetase family protein / COBRA cell expansion protein COBL4 similar to phytochelatin synthetase [Hordeum vulgare subsp. vulgare] GI:29570314; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 431 Score = 27.1 bits (57), Expect = 4.9 Identities = 16/59 (27%), Positives = 30/59 (50%) Frame = +1 Query: 169 SAVFSETVTTSLTMTSGYLMERSALRKASR*RVPSGPQLSTGASRPNTPITSCTMHPSP 345 S+ +++T+T + G ++S ++ S+ G L+T + NTP+ CT H P Sbjct: 222 SSFYNDTITPCPSCACGCENKKSCVKADSKILTKKG--LNT-PKKDNTPLLQCTHHMCP 277 >At4g23720.1 68417.m03413 expressed protein ; expression supported by MPSS Length = 294 Score = 27.1 bits (57), Expect = 4.9 Identities = 19/80 (23%), Positives = 36/80 (45%), Gaps = 2/80 (2%) Frame = +3 Query: 81 LAHWTASWPSAHLNLRCATLKLTASTWVNFRGLLGDGNNEPYDDFRLPNGKICTSESES- 257 L +W+ W + N++ LK+ V+FR L + + E R + K T + E+ Sbjct: 122 LGNWSNHWYTVPDNVKEIILKMKNPVEVSFRDLPKERDEEMLSRVRCVSFKAQTKDEEAT 181 Query: 258 -VTRTVWPAAVHRCKPPEHS 314 ++R V P + ++S Sbjct: 182 HISRMVIPGVCYGSSHGDYS 201 >At3g52115.1 68416.m05720 hypothetical protein Length = 588 Score = 27.1 bits (57), Expect = 4.9 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +3 Query: 105 PSAHLNLRCATLKLTASTWVNFRGLLGDGNNEPYDDF 215 P A + + LK AS W + R G ++P+DDF Sbjct: 422 PGAAKPPQLSGLKRPASIWRDTRSRQSPGGHDPHDDF 458 >At3g44340.1 68416.m04764 sec23/sec24 transport family protein contains Pfam domains PF04811: Sec23/Sec24 trunk domain, PF04815: Sec23/Sec24 helical domain and PF04810: Sec23/Sec24 zinc finger Length = 1096 Score = 27.1 bits (57), Expect = 4.9 Identities = 13/38 (34%), Positives = 17/38 (44%) Frame = +1 Query: 268 PSGPQLSTGASRPNTPITSCTMHPSPPPANRXSGEYRR 381 P G + G N+P++S H PPP N Y R Sbjct: 251 PYGQPPNAGPFTGNSPLSSPPAHSIPPPTNFPGVPYGR 288 >At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative similar to SP|P17859 Alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo}, alpha-amylase [Malus x domestica] GI:7532799; contains Pfam profile PF00128: Alpha amylase, catalytic domain Length = 887 Score = 27.1 bits (57), Expect = 4.9 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = +3 Query: 129 CATLKLTASTWVNFRG 176 C LKL +TW+N+RG Sbjct: 363 CFVLKLNENTWLNYRG 378 >At4g11530.1 68417.m01850 protein kinase family protein contains Pfam PF00069: Protein kinase domain Length = 931 Score = 26.6 bits (56), Expect = 6.5 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 4/37 (10%) Frame = +2 Query: 305 RTLPSPAVRCIPPP---RLRTGLRGNIAA-QDPVIVM 403 ++LP P V IPPP R T ++G I A P+IV+ Sbjct: 523 QSLPQPPVSLIPPPVSDRANTTIKGIIVAIVVPIIVI 559 >At2g11090.1 68415.m01187 expressed protein Length = 151 Score = 26.6 bits (56), Expect = 6.5 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +1 Query: 256 R*RVPSGPQLSTGASRPNTPITSCTMHPSPP 348 R RVPS P L ++P+T I +H P Sbjct: 74 RGRVPSSPSLDHSTTKPSTTIPITRLHTRLP 104 >At1g79800.1 68414.m09316 plastocyanin-like domain-containing protein Length = 192 Score = 26.6 bits (56), Expect = 6.5 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +3 Query: 309 HSHHQLYDASLPPACEQXFGGISPLRTLSLS 401 H HH +DAS+PP+ SP + S S Sbjct: 136 HQHHD-HDASMPPSMSPLSNSASPYASASAS 165 >At1g55940.1 68414.m06416 cytochrome P450, putative similar to SP:Q42569 from [Arabidopsis thaliana] Length = 639 Score = 26.6 bits (56), Expect = 6.5 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 46 VRFPSTKQQNRCWLIGRPHGLLHI 117 V F KQ+NRC+++ P L+ I Sbjct: 238 VNFEILKQENRCFIMSYPEALVRI 261 >At1g19640.1 68414.m02448 S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase (JMT) nearly identical to gi:13676829 Length = 389 Score = 26.6 bits (56), Expect = 6.5 Identities = 13/21 (61%), Positives = 13/21 (61%), Gaps = 1/21 (4%) Frame = -1 Query: 350 GGGEGCIVQLVMGVF-GRLAP 291 GGGE C V V G F GRL P Sbjct: 129 GGGESCFVSAVPGSFYGRLFP 149 >At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase family protein similar to iturin A synthetase C [Bacillus subtilis] GI:16040972; contains Pfam profile PF00501: AMP-binding enzyme Length = 1040 Score = 26.2 bits (55), Expect = 8.6 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -1 Query: 92 PMSQHRFCCLVDGKRTHPFR*SGILWKLRS 3 P +Q+ CCLVDG+ I+W+ R+ Sbjct: 871 PSTQNVICCLVDGQVIAMSPSGTIIWRYRT 900 >At3g56590.1 68416.m06293 hydroxyproline-rich glycoprotein family protein Length = 477 Score = 26.2 bits (55), Expect = 8.6 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +1 Query: 280 QLSTGASRPNTPITSCTMHPSPPPANRXSGEYRRSGPCHCH 402 Q+ + P++P TS T PSP P +Y P H H Sbjct: 305 QVRLSSILPHSPATSSTPSPSPQPETH---QYPHHHPHHHH 342 >At3g48860.2 68416.m05337 expressed protein Length = 577 Score = 26.2 bits (55), Expect = 8.6 Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 1/52 (1%) Frame = +2 Query: 239 HFGKRVGNAYRLARSC-PQVQAARTLPSPAVRCIPPPRLRTGLRGNIAAQDP 391 +F ++V ++ R A + P + A T P+P +PP R+ +I DP Sbjct: 136 NFAEQVPSSVRSASAGRPSMSARSTTPTPIPNLMPPSRVSVKTPVSIPPLDP 187 >At3g48860.1 68416.m05336 expressed protein Length = 494 Score = 26.2 bits (55), Expect = 8.6 Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 1/52 (1%) Frame = +2 Query: 239 HFGKRVGNAYRLARSC-PQVQAARTLPSPAVRCIPPPRLRTGLRGNIAAQDP 391 +F ++V ++ R A + P + A T P+P +PP R+ +I DP Sbjct: 136 NFAEQVPSSVRSASAGRPSMSARSTTPTPIPNLMPPSRVSVKTPVSIPPLDP 187 >At2g16630.1 68415.m01909 proline-rich family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 359 Score = 26.2 bits (55), Expect = 8.6 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = +2 Query: 284 CPQVQAARTLPSPAVRCIPPPRL 352 CP+ A +P P V +PPP++ Sbjct: 148 CPKPPTAPVMPPPQVPVMPPPQV 170 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,018,082 Number of Sequences: 28952 Number of extensions: 214031 Number of successful extensions: 767 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 720 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 764 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 615542944 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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