Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20325
(658 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 69 8e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 54 1e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 47 3e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 1e-05
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.005
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 35 0.009
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.016
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.063
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 32 0.084
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 28 1.0
SPBC947.13 |rba50||RNA polymerase II associated protein |Schizos... 27 2.4
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 27 2.4
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.4
SPBC31F10.04c |srb4|med17|mediator complex subunit Srb4|Schizosa... 26 5.5
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 7.3
SPAC7D4.12c |||DUF1212 family protein|Schizosaccharomyces pombe|... 25 7.3
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 25 7.3
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 9.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 68.5 bits (160), Expect = 8e-13
Identities = 25/65 (38%), Positives = 48/65 (73%)
Frame = +2
Query: 305 QQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 484
++E++EAF+++D++GNGYIT L +L +L ++LS ++ +I E DTDG G ++++EF
Sbjct: 84 EEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
Query: 485 MEMMT 499
+++
Sbjct: 144 SRVIS 148
Score = 62.1 bits (144), Expect = 7e-11
Identities = 27/62 (43%), Positives = 43/62 (69%)
Frame = +2
Query: 311 ELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 490
E +EAF L+DR+ +G IT++ L ++ +L + A+L +I E+D DG+GT+DF EF+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 491 MM 496
MM
Sbjct: 73 MM 74
Score = 46.8 bits (106), Expect = 3e-06
Identities = 21/65 (32%), Positives = 42/65 (64%)
Frame = +3
Query: 57 DSDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSG 236
D+D+E+++ R+AF++FD +GYI V +++ +L ++G+ E+ +I E D + G
Sbjct: 80 DTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136
Query: 237 KINFD 251
IN++
Sbjct: 137 VINYE 141
Score = 44.4 bits (100), Expect = 1e-05
Identities = 20/72 (27%), Positives = 43/72 (59%)
Frame = +3
Query: 69 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 248
++++A R+AF +FD + G I ++ ++ ++GQ +ELQ +I+E D + +G I+F
Sbjct: 8 DEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67
Query: 249 DGSVILLPISLK 284
+ ++ +K
Sbjct: 68 TEFLTMMARKMK 79
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 54.4 bits (125), Expect = 1e-08
Identities = 21/52 (40%), Positives = 34/52 (65%)
Frame = +3
Query: 93 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 248
KAF++FD SGYI+ K + + T+G+ D+E+Q ++ E DP NSG ++
Sbjct: 82 KAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDY 133
Score = 50.0 bits (114), Expect = 3e-07
Identities = 23/77 (29%), Positives = 48/77 (62%)
Frame = +2
Query: 260 NIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIA 439
+ S+ L E ++E +E +AFR++D++ +GYI T+ + + L +KLS+ ++ ++
Sbjct: 65 SFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQ 121
Query: 440 EIDTDGSGTVDFDEFME 490
E D SG+ D+ +F++
Sbjct: 122 EADPTNSGSFDYYDFVQ 138
Score = 35.9 bits (79), Expect = 0.005
Identities = 20/63 (31%), Positives = 37/63 (58%)
Frame = +2
Query: 311 ELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 490
E+KEAF LYD + +G I TS + +L +L +++A+L A++ + +D +FM
Sbjct: 10 EMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAEL----AKLSNELGDAIDEKKFMS 65
Query: 491 MMT 499
++
Sbjct: 66 FVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 46.8 bits (106), Expect = 3e-06
Identities = 19/63 (30%), Positives = 37/63 (58%)
Frame = +2
Query: 308 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFM 487
+E+K AF L+D + G I+ L+ + L++ + + +L+ +I E D D G ++ EF+
Sbjct: 110 EEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Query: 488 EMM 496
+M
Sbjct: 170 AIM 172
Score = 44.4 bits (100), Expect = 1e-05
Identities = 17/53 (32%), Positives = 34/53 (64%)
Frame = +3
Query: 87 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKIN 245
+++AF++FD ++G I + + + + + DD EL+A+I+E D + G+IN
Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEIN 164
Score = 42.3 bits (95), Expect = 6e-05
Identities = 20/74 (27%), Positives = 39/74 (52%)
Frame = +2
Query: 278 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDG 457
L E E +Q++ EAF+L+D + + I L+ + AL +++ I+ + D G
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86
Query: 458 SGTVDFDEFMEMMT 499
G + ++F+ +MT
Sbjct: 87 KGYLQMEDFVRVMT 100
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.4 bits (100), Expect = 1e-05
Identities = 23/72 (31%), Positives = 43/72 (59%), Gaps = 6/72 (8%)
Frame = +2
Query: 305 QQELKEAFRLYDREGNGYITTSTLKEILAAL------DDKLSNADLDGIIAEIDTDGSGT 466
+++LK AF++YD + +GYI+ L +L + +D+L +D I E+D D G
Sbjct: 92 EEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQI-VDKTIMEVDKDRDGK 150
Query: 467 VDFDEFMEMMTG 502
+ F+EF ++++G
Sbjct: 151 ISFEEFKDIVSG 162
Score = 27.9 bits (59), Expect = 1.4
Identities = 15/64 (23%), Positives = 31/64 (48%)
Frame = +2
Query: 308 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFM 487
+ +++ F D +G I + I + + L++ + + +D DG G VDF EF+
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLASR----LFSVVDEDGGGDVDFQEFI 79
Query: 488 EMMT 499
++
Sbjct: 80 NSLS 83
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.9 bits (79), Expect = 0.005
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +2
Query: 308 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 448
QELKEAF L D++G+G I +K +L +L+ S ++ + I+
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 35.1 bits (77), Expect = 0.009
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = +2
Query: 317 KEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 496
K+AF L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 34.3 bits (75), Expect = 0.016
Identities = 21/64 (32%), Positives = 32/64 (50%)
Frame = +2
Query: 302 MQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDE 481
M +E +EAF L+D GYI L+ A L + L+ L ++ T+G V +E
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREE 67
Query: 482 FMEM 493
F E+
Sbjct: 68 FAEL 71
Score = 30.7 bits (66), Expect = 0.19
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +3
Query: 93 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALID 212
+AF +FD T GYID + +G+ +LQ ++D
Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLD 55
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 32.3 bits (70), Expect = 0.063
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +2
Query: 284 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 382
++D M Q LKE Y+ E N IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 31.9 bits (69), Expect = 0.084
Identities = 22/55 (40%), Positives = 29/55 (52%)
Frame = +2
Query: 320 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 484
E FR + E NGY++ S +L + KLS+ L+ I D D G DFDEF
Sbjct: 11 EIFRGLNPE-NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 28.3 bits (60), Expect = 1.0
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = +3
Query: 81 AMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFD 251
A F +FD K+GYID + L+ + + +L D +N+G I++D
Sbjct: 63 AFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYD 119
>SPBC947.13 |rba50||RNA polymerase II associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 27.1 bits (57), Expect = 2.4
Identities = 11/42 (26%), Positives = 23/42 (54%)
Frame = -1
Query: 586 VLVIFNTIYKINTNHNKTIVNPCFSGLVSSHHLHELIKVHSS 461
+ I IY++N+ + +++P LV H++EL+ +S
Sbjct: 380 IQTIGRIIYRLNSGEFREVLSPELHTLVEDAHIYELLAAAAS 421
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 27.1 bits (57), Expect = 2.4
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +3
Query: 141 LKISTILNTMGQLFDDSELQALIDENDPENSGK 239
+ +ST+ +T + DDS L+ +ID+ PE GK
Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGK 43
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/59 (30%), Positives = 23/59 (38%)
Frame = +2
Query: 320 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 496
+ F D E GYI A L + +L I +DT G +D DEF M
Sbjct: 306 QLFSKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362
>SPBC31F10.04c |srb4|med17|mediator complex subunit
Srb4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 545
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +3
Query: 177 LFDDSELQALIDENDPENSGKINFDGSVILLPIS---LKRKTLKPCNKN 314
LF+ +L+ E + +NF G IL+PIS + R TL+ +KN
Sbjct: 305 LFEMDIWNSLLQEAQSCGNQGVNFTGDEILVPISDDHVVRITLETSSKN 353
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 7.3
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +2
Query: 341 REGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 448
RE GY+ T + D L+NA+L I+A+ID
Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142
>SPAC7D4.12c |||DUF1212 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 759
Score = 25.4 bits (53), Expect = 7.3
Identities = 16/73 (21%), Positives = 39/73 (53%), Gaps = 2/73 (2%)
Frame = +3
Query: 51 NMDSDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTI--LNTMGQLFDDSELQALIDENDP 224
N++S +E + +A+++ + ++ ++++ + N G+ D+E IDE+DP
Sbjct: 150 NVESTNELP-STTTEAYKLVAAHTAARLEHMRLNALKAANDEGEDVKDNEQDDNIDESDP 208
Query: 225 ENSGKINFDGSVI 263
+ K ++ G V+
Sbjct: 209 FANPKASYRGGVL 221
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 25.4 bits (53), Expect = 7.3
Identities = 11/44 (25%), Positives = 22/44 (50%)
Frame = +3
Query: 174 QLFDDSELQALIDENDPENSGKINFDGSVILLPISLKRKTLKPC 305
QLFD + Q+ + + N G++NF ++ +K+ + C
Sbjct: 797 QLFDANRQQSPNNSSSDMNEGEMNFQNPTLVNAFEVKQPKMLMC 840
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1183
Score = 25.0 bits (52), Expect = 9.6
Identities = 11/36 (30%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +2
Query: 332 LYDREGNGYITT-STLKEILAALDDKLSNADLDGII 436
LYD+E GY+T + E++ + D+ N D + ++
Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVV 1090
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,554,402
Number of Sequences: 5004
Number of extensions: 49850
Number of successful extensions: 175
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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