Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20323
(445 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 31 0.080
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 1.3
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 27 1.7
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 26 2.3
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 26 3.0
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 26 3.0
SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 25 4.0
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 25 5.2
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy... 25 6.9
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 6.9
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 6.9
SPCC569.05c |||spermidine family transporter |Schizosaccharomyce... 24 9.1
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 24 9.1
SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccha... 24 9.1
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 31.1 bits (67), Expect = 0.080
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +2
Query: 107 YDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRVEATSIRYLFTC 286
Y+ P ++S + +L S L LD++ K L+L FP++I + RV + F C
Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRVFFGQLMKHFYC 210
Query: 287 L 289
L
Sbjct: 211 L 211
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.1 bits (57), Expect = 1.3
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +3
Query: 60 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 155
P H+TT R I +I I PS+LT G +C HI
Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 26.6 bits (56), Expect = 1.7
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +2
Query: 98 YHHYDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRV 253
+ Y F +E +T S + NL E+++L NM+ EL KFP + GR+
Sbjct: 1484 FTQYRLFDVRGKERTSNTMSTY-NL-EEVEYLVNMVDELLNKFPDVNFTGRI 1533
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 26.2 bits (55), Expect = 2.3
Identities = 19/59 (32%), Positives = 35/59 (59%), Gaps = 5/59 (8%)
Frame = +2
Query: 194 DNMMKELSLKFPSII-NEGRVEA-TSI---RYLFTCLVTNRKTST*KRKMEC*WCRLTV 355
+N++KE++ K+ SII N+ ++++ TSI YL+ V K ++K+ W +TV
Sbjct: 6 NNLLKEVAAKYASIILNDAKIKSLTSINSAEYLYDSFVEISKLPL-EKKLPLKWKLITV 63
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 25.8 bits (54), Expect = 3.0
Identities = 10/40 (25%), Positives = 21/40 (52%)
Frame = +2
Query: 134 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRV 253
E + + +W+++ +MQ D ++L F I N G++
Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGKL 143
>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 920
Score = 25.8 bits (54), Expect = 3.0
Identities = 19/89 (21%), Positives = 42/89 (47%), Gaps = 1/89 (1%)
Frame = -2
Query: 402 IHIPGKVLYFQVMIKSTVSLHHQHSIFRFHVD-VFLFVTRQVNRYLILVASTRPSFIMLG 226
+ +P + L F + ++T+ L H S + ++ ++ + R +++ S + +F
Sbjct: 776 LSVPEQELPF-TLEQATMDLRHVRSNYLYNEGYLYRLEDSSIRRRFVVLRSKQLNFYAEK 834
Query: 225 NFNDSSFIMLSKCCISLARLDQSECVSNM 139
LSK +S+ ++ SE VSN+
Sbjct: 835 GGQYLDTFQLSKTVVSIPMVNFSEAVSNL 863
>SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 4.0
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +2
Query: 47 AAVSAAPQYYHGSSHWPYHHYDPFSP 124
A +A PQYY +P HY P P
Sbjct: 502 ATDAAKPQYYIPKDPYPVPHYYPQQP 527
>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
3|||Manual
Length = 872
Score = 25.0 bits (52), Expect = 5.2
Identities = 7/25 (28%), Positives = 16/25 (64%)
Frame = +2
Query: 98 YHHYDPFSPYVRESMLDTHSLWSNL 172
+ H+DP+S ++ ++ + H W+ L
Sbjct: 292 FFHFDPYSLFISNNLEEIHINWNIL 316
>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 794
Score = 24.6 bits (51), Expect = 6.9
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +1
Query: 136 KHVGHTFALVQPCQRNATLGQHDEG 210
KH G + L QP Q+NA + +G
Sbjct: 709 KHEGRSETLAQPGQQNANQSEEKQG 733
>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1316
Score = 24.6 bits (51), Expect = 6.9
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +1
Query: 310 NVKAKNGVLMVQANSAF 360
NVKA+ VLM Q SAF
Sbjct: 608 NVKAERAVLMAQEESAF 624
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 24.6 bits (51), Expect = 6.9
Identities = 7/19 (36%), Positives = 12/19 (63%)
Frame = +3
Query: 273 IYSPAWLRTERHQRESEKW 329
++ P WL +H + +EKW
Sbjct: 343 LFLPRWLPCIKHNQHNEKW 361
>SPCC569.05c |||spermidine family transporter |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 576
Score = 24.2 bits (50), Expect = 9.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 50 AVSAAPQYYHGSSHWPYHHYDPFSPYVRESMLDTHSLWSNLA 175
+ SA P+ YH +SH + S Y +S L +HS SNL+
Sbjct: 19 SASAHPELYHTTSH------ESVSTYQLQSQL-SHSATSNLS 53
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 24.2 bits (50), Expect = 9.1
Identities = 14/51 (27%), Positives = 21/51 (41%)
Frame = +2
Query: 134 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRVEATSIRYLFTC 286
E ++D + + +L M KE LK P+I V + LF C
Sbjct: 146 ELLVDIRGISEETTTGVHNLYKMFKENKLKVPAINVNDSVTKSKFDNLFGC 196
>SPCC16C4.08c |skb15||Shk1 kinase binding protein
15|Schizosaccharomyces pombe|chr 3|||Manual
Length = 341
Score = 24.2 bits (50), Expect = 9.1
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = -2
Query: 267 ILVASTRPSFIMLGNFNDSSFIMLSKCCISLARLDQSECVS 145
+L ST P I+ N+SSF+++S+ I +LD + S
Sbjct: 157 VLPLSTIPESILF--LNESSFVIMSRRGIDAFKLDLTSLFS 195
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,876,760
Number of Sequences: 5004
Number of extensions: 36976
Number of successful extensions: 114
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 162176800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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