BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20319 (489 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 27 1.5 SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy... 27 1.5 SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||... 26 2.7 SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit ... 26 2.7 SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||... 26 3.5 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 25 4.6 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 25 6.1 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 8.1 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.1 bits (57), Expect = 1.5 Identities = 25/74 (33%), Positives = 37/74 (50%), Gaps = 6/74 (8%) Frame = +3 Query: 285 RECKNQKRLSQNKSQENKELRGYLTEVVEER---RLRKTKIXHEIKALIXATGNRHDDE* 455 R+ K Q+ QNK +E ELR L E ++ RL ++ E + L A N + + Sbjct: 502 RQLKEQEEDLQNKEEELTELRKLLREQTQDSQKLRLLVEQLELERQDLKQAGENHYSN-- 559 Query: 456 RSSDY---VKKLET 488 SSDY +K LE+ Sbjct: 560 LSSDYETQIKSLES 573 >SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 27.1 bits (57), Expect = 1.5 Identities = 10/28 (35%), Positives = 19/28 (67%) Frame = -2 Query: 107 ICSKIFNLLCIHTFLMYFIHIYVSVDHI 24 ICS +++L + F++ +H+YV V +I Sbjct: 101 ICSNSYSILVNYGFILNMVHMYVHVFNI 128 >SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2|||Manual Length = 525 Score = 26.2 bits (55), Expect = 2.7 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = -3 Query: 298 FLHSLNSIFFNILHMSSLLCPLLFF 224 F+H L+S + N+L++ S +C L F Sbjct: 321 FVHQLSSSYTNLLNLRSAICDWLKF 345 >SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit Sfc4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1006 Score = 26.2 bits (55), Expect = 2.7 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +3 Query: 294 KNQKRLSQNKSQENKELRGYLTEVVEERR 380 + Q + N E ++L GY++E+V+E R Sbjct: 16 ETQNDTTDNFDAEMQDLNGYISEIVDEAR 44 >SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 565 Score = 25.8 bits (54), Expect = 3.5 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = +1 Query: 121 ISNLIYHEIEMAKSKYNIWDDNFSWKN 201 ++NL Y + S ++I D+NFS+ N Sbjct: 368 LTNLPYEFVRFNNSMHSIGDNNFSYNN 394 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 25.4 bits (53), Expect = 4.6 Identities = 13/42 (30%), Positives = 26/42 (61%), Gaps = 2/42 (4%) Frame = +3 Query: 294 KNQKRLSQNKSQENKELRGYLTEVVEERRL--RKTKIXHEIK 413 +++KR + S++N+ELR +E+ +R+L K+ E+K Sbjct: 1272 EDEKRKALIASRDNEELRSLKSELESKRKLEVEYQKVLEEVK 1313 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 25.0 bits (52), Expect = 6.1 Identities = 14/50 (28%), Positives = 25/50 (50%) Frame = -3 Query: 346 RSSLFSWDLFWDNLFWFLHSLNSIFFNILHMSSLLCPLLFFVIK*LDCNF 197 R L WDL + + +++ N LH LL PLL +++ L+ ++ Sbjct: 1322 RVYLLVWDLIFYHFEETTYNIKLSIINQLHAMDLLRPLLNTLVEILNLSY 1371 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 24.6 bits (51), Expect = 8.1 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +3 Query: 33 YTYVNVNEVHEKSVNT 80 Y+ V V+E HE+SVNT Sbjct: 516 YSAVIVDEAHERSVNT 531 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,671,028 Number of Sequences: 5004 Number of extensions: 29272 Number of successful extensions: 103 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 103 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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