BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20314 (412 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 64 1e-11 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 56 3e-09 SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 24 8.0 SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual 24 8.0 SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr 1||... 24 8.0 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 24 8.0 >SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 937 Score = 63.7 bits (148), Expect = 1e-11 Identities = 30/74 (40%), Positives = 42/74 (56%) Frame = +2 Query: 32 MAQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLXXXXX 211 MA ++ G +A + +LR+Q++ ++S F L I+QVGGREDSNVY+RMK Sbjct: 1 MALLLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANE 60 Query: 212 XXXXXEHXRLPRDI 253 EH P DI Sbjct: 61 AGISCEHVNFPEDI 74 Score = 47.2 bits (107), Expect = 1e-06 Identities = 23/49 (46%), Positives = 32/49 (65%) Frame = +1 Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDG 402 E +L I NE P+VHGIIVQ+PL + I+ +T+AV+P+K DG Sbjct: 76 EYDLLLAIKGFNEDPTVHGIIVQLPLPA--HINEQIITEAVAPEKDVDG 122 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 55.6 bits (128), Expect = 3e-09 Identities = 26/53 (49%), Positives = 38/53 (71%) Frame = +2 Query: 35 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMK 193 A I++G +A ++ + ++V L++K F+P LAIVQVG REDSNVY+RMK Sbjct: 33 ANILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMK 85 Score = 37.1 bits (82), Expect = 0.001 Identities = 16/46 (34%), Positives = 29/46 (63%) Frame = +1 Query: 265 LXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDG 402 L ++ LN+ +VHG++VQ+PL ++ VT++++P K DG Sbjct: 110 LLHELKKLNDDHTVHGVLVQLPLPK--HLNERTVTESITPPKDVDG 153 >SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 24.2 bits (50), Expect = 8.0 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = -1 Query: 238 AXMFGGDTNFXSCLQLHPDVDVGVLAPAHLHDGKPGLEAR 119 A F T+F S ++ +PD+ V L L + PG++ + Sbjct: 756 ALSFNHYTHFTSPIRRYPDIIVHRLLERSLKNTSPGIDKK 795 >SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual Length = 503 Score = 24.2 bits (50), Expect = 8.0 Identities = 10/37 (27%), Positives = 19/37 (51%) Frame = +1 Query: 241 TKGHHEIELXAKITSLNESPSVHGIIVQMPLDSDHXI 351 +K H + ++ + L SVH +I ++P DH + Sbjct: 69 SKIHEDQQVSFESVDLTSYESVHSMIERLPFCPDHVV 105 >SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 24.2 bits (50), Expect = 8.0 Identities = 14/46 (30%), Positives = 24/46 (52%) Frame = +2 Query: 56 EVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMK 193 E+ S++ D+RQ++ +L + S E I + RE IRM+ Sbjct: 31 EIESSLK-DVRQELQKLNEEQSRLEKNAQIPEYRVRESEAFLIRMQ 75 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 24.2 bits (50), Expect = 8.0 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +3 Query: 3 RPTRQFSGLRWHKLYLGLK 59 RPTR+ + W KL GL+ Sbjct: 985 RPTRRLKQMHWEKLNSGLE 1003 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,442,046 Number of Sequences: 5004 Number of extensions: 23608 Number of successful extensions: 51 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 48 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 50 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 142254980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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