BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20314 (412 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 45 2e-05 At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd... 45 2e-05 At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd... 43 7e-05 At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd... 33 0.099 At5g46570.1 68418.m05734 protein kinase family protein contains ... 30 0.53 At1g64330.1 68414.m07290 myosin heavy chain-related similar to m... 28 2.8 >At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 360 Score = 45.2 bits (102), Expect = 2e-05 Identities = 22/57 (38%), Positives = 34/57 (59%) Frame = +1 Query: 238 LTKGHHEIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408 L + E E+ ++ N+ PSVHGI+VQ+PL S +D + +AVS +K DG + Sbjct: 136 LAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPS--HMDEQNILNAVSIEKDVDGFH 190 Score = 37.9 bits (84), Expect = 0.003 Identities = 20/53 (37%), Positives = 32/53 (60%) Frame = +2 Query: 35 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMK 193 A +I G VA I +++ +V+R++ G P LA++ VG R+DS Y+R K Sbjct: 69 AIVIDGKAVAKKIRDEITIEVSRMKES-IGVIPGLAVILVGDRKDSATYVRNK 120 >At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 299 Score = 44.8 bits (101), Expect = 2e-05 Identities = 22/51 (43%), Positives = 32/51 (62%) Frame = +1 Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408 E +L +K+ LN +P VHGI+VQ+PL H + H + A+S DK DG + Sbjct: 82 EADLISKVHELNSNPDVHGILVQLPLPK-HINEEH-ILGAISIDKDVDGFH 130 >At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 310 Score = 43.2 bits (97), Expect = 7e-05 Identities = 20/51 (39%), Positives = 32/51 (62%) Frame = +1 Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408 E E+ ++ N+ PSVHG++VQ+PL S +D + +AVS +K DG + Sbjct: 92 EEEVLKYVSGFNDDPSVHGVLVQLPLPS--HMDEQNILNAVSIEKDVDGFH 140 >At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 352 Score = 32.7 bits (71), Expect = 0.099 Identities = 15/51 (29%), Positives = 29/51 (56%) Frame = +1 Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408 E ++ + + NE S+HGI+VQ+PL ++ ++ + V +K DG + Sbjct: 136 EGQIISVLRKFNEDTSIHGILVQLPL--PQHLNESKILNMVRLEKDVDGFH 184 >At5g46570.1 68418.m05734 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 489 Score = 30.3 bits (65), Expect = 0.53 Identities = 18/51 (35%), Positives = 26/51 (50%) Frame = +2 Query: 44 ISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKL 196 ++G +V I+N ++ LR +GF P IV GG + NV R KL Sbjct: 26 VNGDQVDQEIQNFKEFELNELRKATNGFSPS-CIVSEGGEKAPNVVYRGKL 75 >At1g64330.1 68414.m07290 myosin heavy chain-related similar to myosin heavy chain (GI:1850913) [Entamoeba histolytica]; similar to Intracellular protein transport protein USO1 (Swiss-Prot:P25386) [Saccharomyces cerevisiae] Length = 555 Score = 27.9 bits (59), Expect = 2.8 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +2 Query: 53 IEVAGSIENDLRQQVTRLRSKWSGFEPRLA 142 +EVAG E+DL Q++ ++ + G E LA Sbjct: 190 LEVAGETESDLNQKLEDVKKERDGLEAELA 219 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,729,036 Number of Sequences: 28952 Number of extensions: 130937 Number of successful extensions: 306 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 305 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 306 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 615542944 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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