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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20314
         (412 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd...    45   2e-05
At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd...    45   2e-05
At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd...    43   7e-05
At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd...    33   0.099
At5g46570.1 68418.m05734 protein kinase family protein contains ...    30   0.53 
At1g64330.1 68414.m07290 myosin heavy chain-related similar to m...    28   2.8  

>At4g00620.1 68417.m00086 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 360

 Score = 45.2 bits (102), Expect = 2e-05
 Identities = 22/57 (38%), Positives = 34/57 (59%)
 Frame = +1

Query: 238 LTKGHHEIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408
           L +   E E+   ++  N+ PSVHGI+VQ+PL S   +D   + +AVS +K  DG +
Sbjct: 136 LAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPS--HMDEQNILNAVSIEKDVDGFH 190



 Score = 37.9 bits (84), Expect = 0.003
 Identities = 20/53 (37%), Positives = 32/53 (60%)
 Frame = +2

Query: 35  AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMK 193
           A +I G  VA  I +++  +V+R++    G  P LA++ VG R+DS  Y+R K
Sbjct: 69  AIVIDGKAVAKKIRDEITIEVSRMKES-IGVIPGLAVILVGDRKDSATYVRNK 120


>At3g12290.1 68416.m01534 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 299

 Score = 44.8 bits (101), Expect = 2e-05
 Identities = 22/51 (43%), Positives = 32/51 (62%)
 Frame = +1

Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408
           E +L +K+  LN +P VHGI+VQ+PL   H  + H +  A+S DK  DG +
Sbjct: 82  EADLISKVHELNSNPDVHGILVQLPLPK-HINEEH-ILGAISIDKDVDGFH 130


>At4g00600.1 68417.m00084 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
           precursor (C1-THF synthase) [Includes:
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
           Formyltetrahydrofolate synthetase (EC 6.3.4.3)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           NAD(P)-binding domain, PF00763: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, catalytic domain
          Length = 310

 Score = 43.2 bits (97), Expect = 7e-05
 Identities = 20/51 (39%), Positives = 32/51 (62%)
 Frame = +1

Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408
           E E+   ++  N+ PSVHG++VQ+PL S   +D   + +AVS +K  DG +
Sbjct: 92  EEEVLKYVSGFNDDPSVHGVLVQLPLPS--HMDEQNILNAVSIEKDVDGFH 140


>At2g38660.1 68415.m04748 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
           precursor (C1-THF synthase) [Includes:
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
           Formyltetrahydrofolate synthetase (EC 6.3.4.3)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           NAD(P)-binding domain, PF00763: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, catalytic domain
          Length = 352

 Score = 32.7 bits (71), Expect = 0.099
 Identities = 15/51 (29%), Positives = 29/51 (56%)
 Frame = +1

Query: 256 EIELXAKITSLNESPSVHGIIVQMPLDSDHXIDAHRVTDAVSPDKXXDGLN 408
           E ++ + +   NE  S+HGI+VQ+PL     ++  ++ + V  +K  DG +
Sbjct: 136 EGQIISVLRKFNEDTSIHGILVQLPL--PQHLNESKILNMVRLEKDVDGFH 184


>At5g46570.1 68418.m05734 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 489

 Score = 30.3 bits (65), Expect = 0.53
 Identities = 18/51 (35%), Positives = 26/51 (50%)
 Frame = +2

Query: 44  ISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKL 196
           ++G +V   I+N    ++  LR   +GF P   IV  GG +  NV  R KL
Sbjct: 26  VNGDQVDQEIQNFKEFELNELRKATNGFSPS-CIVSEGGEKAPNVVYRGKL 75


>At1g64330.1 68414.m07290 myosin heavy chain-related similar to
           myosin heavy chain (GI:1850913) [Entamoeba histolytica];
           similar to Intracellular protein transport protein USO1
           (Swiss-Prot:P25386) [Saccharomyces cerevisiae]
          Length = 555

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +2

Query: 53  IEVAGSIENDLRQQVTRLRSKWSGFEPRLA 142
           +EVAG  E+DL Q++  ++ +  G E  LA
Sbjct: 190 LEVAGETESDLNQKLEDVKKERDGLEAELA 219


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,729,036
Number of Sequences: 28952
Number of extensions: 130937
Number of successful extensions: 306
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 305
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 306
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 615542944
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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