Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20311
         (589 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g53760.1 68416.m05939 tubulin family protein similar to SP|Q9...    38   0.004
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9...    35   0.035
At3g16620.1 68416.m02124 chloroplast outer membrane protein, put...    31   0.75 
At1g70400.1 68414.m08098 hypothetical protein similar to U4/U6 s...    30   1.00 
At4g07960.1 68417.m01276 glycosyl transferase family 2 protein s...    29   3.0  
At3g44160.1 68416.m04734 chloroplast outer membrane protein-rela...    29   3.0  
At5g56800.1 68418.m07088 hypothetical protein                          28   5.3  
At3g43610.1 68416.m04640 tubulin family protein                        27   7.0  
At5g11200.1 68418.m01309 DEAD/DEAH box helicase, putative              27   9.3  
At5g11170.2 68418.m01306 DEAD/DEAH box helicase, putative (RH15)...    27   9.3  
At5g11170.1 68418.m01305 DEAD/DEAH box helicase, putative (RH15)...    27   9.3  
At3g50150.1 68416.m05482 expressed protein contains Pfam profile...    27   9.3  
At3g47560.1 68416.m05177 esterase/lipase/thioesterase family pro...    27   9.3  
At1g60200.1 68414.m06781 splicing factor PWI domain-containing p...    27   9.3  
At1g31240.1 68414.m03823 expressed protein identical to hypothet...    27   9.3  

>At3g53760.1 68416.m05939 tubulin family protein similar to
           SP|Q9SC88 Gamma-tubulin complex component 4 homolog
           {Medicago truncatula}, SP|Q9UGJ1|GCP4_HUMAN
           Gamma-tubulin complex component 4 {Homo sapiens};
           contains Pfam profile PF04130: Spc97 / Spc98 family
          Length = 745

 Score = 38.3 bits (85), Expect = 0.004
 Identities = 20/67 (29%), Positives = 36/67 (53%)
 Frame = +2

Query: 209 RKEINGLSDIEFNSSNILSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFF 388
           R +I G   +   +     G   +   + ++  + ++V  +QL  W++YG L+D + EFF
Sbjct: 173 RDDIRGGQLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFF 232

Query: 389 ISKRKDD 409
           I KR+DD
Sbjct: 233 I-KRQDD 238


>At5g06680.1 68418.m00754 tubulin family protein similar to
           SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
           sapiens}
          Length = 838

 Score = 35.1 bits (77), Expect = 0.035
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +2

Query: 266 GNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFFI 391
           G+  +HD ++ +   V       + +W+L GEL+D + EFF+
Sbjct: 350 GDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFV 391


>At3g16620.1 68416.m02124 chloroplast outer membrane protein,
           putative similar to chloroplast protein import component
           Toc159 [Pisum sativum] GI:8489806, chloroplast outer
           envelope protein 86 [Pisum sativum] GI:599958,
           GTP-binding protein [Pisum sativum] GI:576509
          Length = 1089

 Score = 30.7 bits (66), Expect = 0.75
 Identities = 12/24 (50%), Positives = 19/24 (79%)
 Frame = +2

Query: 221 NGLSDIEFNSSNILSGNKSIHDAV 292
           NG +  EFNS  I+SG+KS++D++
Sbjct: 226 NGKTGAEFNSVKIVSGDKSLNDSI 249


>At1g70400.1 68414.m08098 hypothetical protein similar to U4/U6
           snRNP-associated 61 kDa protein [Homo sapiens]
           GI:18249847
          Length = 168

 Score = 30.3 bits (65), Expect = 1.00
 Identities = 18/63 (28%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
 Frame = +3

Query: 12  SHRYGLYTQALSEGITEVLLSYKKTLVEVESLVIGNHNYTLSYVYSYI-EKYQGLFNTLN 188
           S RY    Q + E +   +L YKK +V+ + L++   N  +  V ++I +KY+  F  L 
Sbjct: 21  SRRYADIMQQVEEALEGSVLEYKKLIVDCKQLLVDIEN-EIVIVQNFIRDKYRVKFQELE 79

Query: 189 RII 197
            ++
Sbjct: 80  LLV 82


>At4g07960.1 68417.m01276 glycosyl transferase family 2 protein
           similar to cellulose synthase from Agrobacterium
           tumeficiens [gi:710492] and Agrobacterium radiobacter
           [gi:710493]; contains Pfam glycosyl transferase, group 2
           family protein domain PF00535
          Length = 699

 Score = 28.7 bits (61), Expect = 3.0
 Identities = 16/40 (40%), Positives = 22/40 (55%)
 Frame = +2

Query: 209 RKEINGLSDIEFNSSNILSGNKSIHDAVLKIFTSVNKVFI 328
           R+   G +DIE +SS + S  K  H    K+F S  KVF+
Sbjct: 92  RRIAAGRTDIEISSSGVGSLQKQNHTKKSKLFYSCLKVFL 131


>At3g44160.1 68416.m04734 chloroplast outer membrane protein-related
           low similarity to chloroplastic outer envelope membrane
           protein (OEP75) [Pisum sativum] GI:633607
          Length = 362

 Score = 28.7 bits (61), Expect = 3.0
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +2

Query: 260 LSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKD 370
           +   KSIH    KI   VN  F H+LCT L++   +D
Sbjct: 1   MGAQKSIHAGRAKI--DVNVDFTHKLCTSLMFPAFRD 35


>At5g56800.1 68418.m07088 hypothetical protein
          Length = 344

 Score = 27.9 bits (59), Expect = 5.3
 Identities = 21/54 (38%), Positives = 29/54 (53%)
 Frame = -3

Query: 320 LCSHL*IFSKRHHESTCCHLKYLMN*TQYLTAH*SPFFNSCYNSVQCIEQTLIF 159
           L SHL IF  RH++ T    K      +Y+ A  S    + ++SV CIE+ LIF
Sbjct: 278 LSSHLEIFEWRHYKGT----KQERKVAKYILAKASCLKVAIFSSV-CIEKNLIF 326


>At3g43610.1 68416.m04640 tubulin family protein
          Length = 1120

 Score = 27.5 bits (58), Expect = 7.0
 Identities = 12/43 (27%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = +2

Query: 281 HDAVLK-IFTSVNKVFIHQLCTWLLYGELKDVYQEFFISKRKD 406
           H A+LK +F    + +   + +W+   EL D ++EF +  R +
Sbjct: 291 HSAMLKFLFLKTCEPYCEFIRSWMFKAELNDPHKEFIVECRSE 333


>At5g11200.1 68418.m01309 DEAD/DEAH box helicase, putative
          Length = 427

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
 Frame = +3

Query: 369 MCIKSSSYQKEKMTRQKLYCQHHLLLMSVTKLL--FLHCRRH*VVVTHS--SNTDTTFHA 536
           +CI S   Q+E++TR K + + H  ++  T L+   +   R  +V+ +    + DT  H 
Sbjct: 316 ICIHSGMSQEERLTRYKSFKEGHKRILVATDLVGRGIDIERVNIVINYDMPDSADTYLHR 375

Query: 537 I 539
           +
Sbjct: 376 V 376


>At5g11170.2 68418.m01306 DEAD/DEAH box helicase, putative (RH15)
           DEAD BOX RNA helicase RH15, Arabidopsis thaliana,
           EMBL:ATH010466
          Length = 344

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
 Frame = +3

Query: 369 MCIKSSSYQKEKMTRQKLYCQHHLLLMSVTKLL--FLHCRRH*VVVTHS--SNTDTTFHA 536
           +CI S   Q+E++TR K + + H  ++  T L+   +   R  +V+ +    + DT  H 
Sbjct: 233 ICIHSGMSQEERLTRYKSFKEGHKRILVATDLVGRGIDIERVNIVINYDMPDSADTYLHR 292

Query: 537 I 539
           +
Sbjct: 293 V 293


>At5g11170.1 68418.m01305 DEAD/DEAH box helicase, putative (RH15)
           DEAD BOX RNA helicase RH15, Arabidopsis thaliana,
           EMBL:ATH010466
          Length = 427

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
 Frame = +3

Query: 369 MCIKSSSYQKEKMTRQKLYCQHHLLLMSVTKLL--FLHCRRH*VVVTHS--SNTDTTFHA 536
           +CI S   Q+E++TR K + + H  ++  T L+   +   R  +V+ +    + DT  H 
Sbjct: 316 ICIHSGMSQEERLTRYKSFKEGHKRILVATDLVGRGIDIERVNIVINYDMPDSADTYLHR 375

Query: 537 I 539
           +
Sbjct: 376 V 376


>At3g50150.1 68416.m05482 expressed protein contains Pfam profile
           PF03140: Plant protein of unknown function; expression
           supported by MPSS
          Length = 509

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = +2

Query: 200 NY*RKEINGLSDIEFNSSNILSGNKSIHDAVLKIFTSV 313
           N+ RKE   L DIEF +  +      IHD    +F+++
Sbjct: 340 NFMRKETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNL 377


>At3g47560.1 68416.m05177 esterase/lipase/thioesterase family
           protein low similarity to cinnamoyl ester hydrolase CinI
           [Butyrivibrio fibrisolvens] GI:1622732; contains
           Interpro entry IPR000379
          Length = 265

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 16/53 (30%), Positives = 25/53 (47%)
 Frame = +3

Query: 48  EGITEVLLSYKKTLVEVESLVIGNHNYTLSYVYSYIEKYQGLFNTLNRIITTI 206
           EGI+     +        S   GN+NY    ++S I+     F+ LNR++T I
Sbjct: 60  EGISAFRFDFSGNGESEGSFYYGNYNYEADDLHSVIQ----YFSNLNRVVTII 108


>At1g60200.1 68414.m06781 splicing factor PWI domain-containing
           protein / RNA recognition motif (RRM)-containing protein
           contains Pfam profiles PF01480: PWI domain, PF00076: RNA
           recognition motif. (a.k.a. RRM, RBD, or RNP domain)
          Length = 899

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 11/47 (23%), Positives = 23/47 (48%)
 Frame = +3

Query: 21  YGLYTQALSEGITEVLLSYKKTLVEVESLVIGNHNYTLSYVYSYIEK 161
           +G Y    +EGI   +    +  ++ + L++  +  T  Y+  Y+EK
Sbjct: 246 FGFYEFESAEGILRAIRLLTQRTIDGQELLVNVNQATKEYLLKYVEK 292


>At1g31240.1 68414.m03823 expressed protein identical to
           hypothetical protein GB:AAD21690 GI:4512621 from
           (Arabidopsis thaliana)
          Length = 277

 Score = 27.1 bits (57), Expect = 9.3
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = +2

Query: 218 INGLSDIEFNSSNILSGNKSIHD 286
           +NGL DI  ++S+   G  ++HD
Sbjct: 88  VNGLQDIALSTSDCFPGGSTVHD 110


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,952,134
Number of Sequences: 28952
Number of extensions: 228389
Number of successful extensions: 475
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 463
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 475
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)