BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20297
(534 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 34 0.052
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 31 0.48
At3g10180.1 68416.m01219 kinesin motor protein-related similar t... 31 0.64
At3g57780.1 68416.m06436 expressed protein 30 1.1
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 29 1.5
At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative si... 29 1.5
At1g23230.1 68414.m02906 expressed protein 29 1.9
At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase f... 29 2.6
At3g45530.1 68416.m04917 DC1 domain-containing protein contains ... 29 2.6
At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containi... 29 2.6
At3g10720.2 68416.m01291 pectinesterase, putative contains simil... 28 3.4
At2g02720.1 68415.m00214 pectate lyase family protein similar to... 28 3.4
At1g78400.1 68414.m09136 glycoside hydrolase family 28 protein /... 27 5.9
At1g49980.1 68414.m05609 UMUC-like DNA repair family protein low... 27 5.9
At5g42490.1 68418.m05172 kinesin motor family protein contains P... 27 7.9
At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein si... 27 7.9
At5g20540.1 68418.m02439 expressed protein 27 7.9
At4g32420.1 68417.m04615 peptidyl-prolyl cis-trans isomerase cyc... 27 7.9
At4g15820.1 68417.m02407 wound-responsive protein-related contai... 27 7.9
At3g28770.1 68416.m03591 expressed protein 27 7.9
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 34.3 bits (75), Expect = 0.052
Identities = 24/71 (33%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
Frame = +2
Query: 80 SPRSPQT*RVSSKASTPAVSVTSTPMKRLCFRLL--KTSPLRRPRSLYSTVSRSLIRAS* 253
SP ++ R S++ P+ S +S R + ++ PL R RSLYS+VSRS R+
Sbjct: 193 SPDDSKSYRSRSRSRGPSCSYSSKSRSVSPARSISPRSRPLSRSRSLYSSVSRSQSRSKS 252
Query: 254 THRDSGEEPAS 286
R P S
Sbjct: 253 RSRSRSNSPVS 263
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 31.1 bits (67), Expect = 0.48
Identities = 30/120 (25%), Positives = 47/120 (39%), Gaps = 4/120 (3%)
Frame = +2
Query: 8 PLPHQKYIDSEWPAP*VTLPP*KT----SPRSPQT*RVSSKASTPAVSVTSTPMKRLCFR 175
P P + + S PA + PP + SP ++ +P+ S S P ++ +
Sbjct: 463 PPPRRAGLPSPPPAQRLPSPPPRRAGLPSPMRIGGSHAANHLESPSPSSLSPPGRK---K 519
Query: 176 LLKTSPLRRPRSLYSTVSRSLIRAS*THRDSGEEPASGQRRYRSGEGKEQIPERHRELRS 355
+L + P+RR RSL R + H G G GK RH++ RS
Sbjct: 520 VLPSPPVRRRRSLTPDEERVSLSQGGRHTSPSHIKQDGSMSPVRGRGKSSPSSRHQKARS 579
>At3g10180.1 68416.m01219 kinesin motor protein-related similar to
centromere protein E GB:4502781 [Homo sapiens]
Length = 1348
Score = 30.7 bits (66), Expect = 0.64
Identities = 21/60 (35%), Positives = 29/60 (48%)
Frame = +3
Query: 255 HTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNSLPTKDVIEQEKSA 434
H E EK+ + + KEK E TKLK TE+ KNS+ K +E EK++
Sbjct: 799 HIECLEKD-IGSLSSSSLAKEKENLRKDFEK-TKTKLKDTESKLKNSMQDKTKLEAEKAS 856
>At3g57780.1 68416.m06436 expressed protein
Length = 670
Score = 29.9 bits (64), Expect = 1.1
Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +3
Query: 243 EPAEHTETQEK-NPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCE 386
+ +E TE +E N L D E EKE++K L+ I + + ETCE
Sbjct: 72 DSSEKTEKEETINGLACDDEDEEEKEESKELDAIAHEKTDSVSSPETCE 120
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 29.5 bits (63), Expect = 1.5
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 252 EHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 353
+ TE E + LPD ++ +K K + +N + N+D
Sbjct: 10 DSTELVENDNLPDSSIVKKKKSKTEKMNTVANYD 43
>At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase isoform 1, Citrus
unshiu, PIR:S72648
Length = 894
Score = 29.5 bits (63), Expect = 1.5
Identities = 26/71 (36%), Positives = 36/71 (50%), Gaps = 6/71 (8%)
Frame = +1
Query: 49 SVSDTPS--LKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVA-TEKTQKSLF 219
S SD+PS L+D+ ++ +LK L+G + VDTN +AE A EK +L
Sbjct: 532 SDSDSPSDSLRDINDISLNLKLSLDGEKSGSNNGVDTNLDAEDRAAERKAEVEKAVSTLA 591
Query: 220 D---GIEKFDS 243
EKFDS
Sbjct: 592 QKSKPTEKFDS 602
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 29.1 bits (62), Expect = 1.9
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = -1
Query: 408 PLWGASSSRTFPCASA*WDRSSRC-RSGICSFPSP 307
PL+G + + P S W+R+ RC R I + PSP
Sbjct: 393 PLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSP 427
>At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase
family protein / UPP synthetase family protein contains
putative undecaprenyl diphosphate synthase domain
[PF01255]; similar to S. cerevisiae dehydrodolichyl
diphosphate synthetase (DEDOL-PP
synthase)(Rer2)[SP|P35196], a cis-prenyltransferase
Length = 271
Score = 28.7 bits (61), Expect = 2.6
Identities = 16/56 (28%), Positives = 29/56 (51%)
Frame = -1
Query: 249 LARIKLLDTVE*RLLGLLSGDVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLG 82
L R+ + + + ++ G+ +KHNL IG+D AG + + LQ C ++G
Sbjct: 25 LFRVLCVGPIPTNISFIMDGNRRFAKKHNL-IGLDAGHRAGFISVKYILQYCKEIG 79
>At3g45530.1 68416.m04917 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 692
Score = 28.7 bits (61), Expect = 2.6
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -3
Query: 454 CYKVVVQADFSCSMT--SFVGSEFFSHVSVCFSLVGSKFSMPF 332
C++ ++ DF C F F+S V F L+ S +MPF
Sbjct: 438 CFESIIDDDFLCRACYQMFCEGFFYSSKGVNFDLICSSITMPF 480
>At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 786
Score = 28.7 bits (61), Expect = 2.6
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +1
Query: 49 SVSDTPSLKDLPKVATDLKSQLEGF---NTSCLRDVDTNEKIVLPSAEDVATEKTQKS 213
S+ + + DLPK+ + SQ E + S +DT E ++ P+AED E S
Sbjct: 78 SLEEVTNADDLPKIDDEKNSQFETSPHPSPSPSVALDTEEGLINPTAEDTVEENIVSS 135
>At3g10720.2 68416.m01291 pectinesterase, putative contains
similarity to pectinesterase from Vitis vinifera
GI:15081598, Prunus persica SP|Q43062; contains Pfam
profile PF01095 pectinesterase
Length = 619
Score = 28.3 bits (60), Expect = 3.4
Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Frame = +2
Query: 11 LPHQKYIDSEWPAP*VTLPP*KTSPRSPQT*RVSSKASTPAVSVTSTPMKRLCFRLL--- 181
LP + ++S + P + PP P P + S++ +P+++ STP +LC +L
Sbjct: 43 LPFEPPVESPFFPP--SQPPIFVPPSQPPS-LPPSQSQSPSLACKSTPYPKLCRTILNAV 99
Query: 182 KTSPLRRPRSLYSTVSRSLIRAS 250
K+SP R T+ + L +AS
Sbjct: 100 KSSPSDPYRYGKFTIKQCLKQAS 122
>At2g02720.1 68415.m00214 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 455
Score = 28.3 bits (60), Expect = 3.4
Identities = 16/61 (26%), Positives = 25/61 (40%)
Frame = +3
Query: 243 EPAEHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNSLPTKDVIEQ 422
E ++ +E P P+ + + EK+ FLNG K KN +P K I
Sbjct: 372 EDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAIRP 431
Query: 423 E 425
+
Sbjct: 432 Q 432
>At1g78400.1 68414.m09136 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
exopolygalacturonase GI:311962 from [Arabidopsis
thaliana]; contains Pfam profile PF00295: Glycosyl
hydrolases family 28 (polygalacturonases)
Length = 404
Score = 27.5 bits (58), Expect = 5.9
Identities = 9/33 (27%), Positives = 21/33 (63%)
Frame = +2
Query: 419 AREISLNHYFITVTRKCISLVSPYFNIDVSKID 517
+ + ++H I CI+++S FN+D++K++
Sbjct: 212 SNHMKIHHVDIATGDDCIAILSGTFNLDINKVN 244
>At1g49980.1 68414.m05609 UMUC-like DNA repair family protein low
similarity to DNA polymerase kappa [Mus musculus]
GI:14279087; contains Pfam profile PF00817:
ImpB/MucB/SamB family
Length = 785
Score = 27.5 bits (58), Expect = 5.9
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -1
Query: 204 GLLSGDVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLGE 79
GL G++ + +++ +T +AGV A L +VC D+ +
Sbjct: 214 GLSGGEIAEELRSSVYSETGLTCSAGVAANRLLAKVCSDINK 255
>At5g42490.1 68418.m05172 kinesin motor family protein contains Pfam
domain, PF00225: Kinesin motor domain
Length = 1087
Score = 27.1 bits (57), Expect = 7.9
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = +3
Query: 243 EPAEHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNSLPTKDVIE 419
E E +E+ ++ + K+ + LN EN ++ + TE CE NSLP + E
Sbjct: 567 ESQESVNEEEQMKNEERKMSPSTKQAEQCLNKEENAQ-SEQQSTEDCELNSLPINNQSE 624
>At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 549
Score = 27.1 bits (57), Expect = 7.9
Identities = 14/57 (24%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +3
Query: 243 EPAEHTETQEKNPLPDKDAIEAEKEKNKF-LNGIENFDPTKLKHTETCEKNSLPTKD 410
E A+ + T++ L D E E ++ E + + E CEK+ + T+D
Sbjct: 178 ETAQSSNTEDSQVLESHDIEEVESSTRTITVDESEKLNSESIMQDENCEKSDVTTED 234
>At5g20540.1 68418.m02439 expressed protein
Length = 384
Score = 27.1 bits (57), Expect = 7.9
Identities = 13/49 (26%), Positives = 24/49 (48%)
Frame = +1
Query: 52 VSDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATE 198
++ TP + + T+ S +S RD D +E++ + +A DV E
Sbjct: 282 LNSTPKVSSISVAKTETSSIDASIRSSSSRDADRSEEMSVSNASDVDNE 330
>At4g32420.1 68417.m04615 peptidyl-prolyl cis-trans isomerase
cyclophilin-type family protein weak similarity to
CARS-Cyp [Homo sapiens] GI:1117968; contains Pfam
profile PF00160: peptidyl-prolyl cis-trans isomerase,
cyclophilin-type
Length = 837
Score = 27.1 bits (57), Expect = 7.9
Identities = 20/54 (37%), Positives = 28/54 (51%)
Frame = +2
Query: 137 SVTSTPMKRLCFRLLKTSPLRRPRSLYSTVSRSLIRAS*THRDSGEEPASGQRR 298
S++ +P RL R L SP+R PR +VSRS +R+S + P RR
Sbjct: 546 SLSRSPT-RLSRRSLSRSPIRSPR---KSVSRSPVRSS--RKSVSRSPVRSSRR 593
>At4g15820.1 68417.m02407 wound-responsive protein-related contains
weak similarity to KED [Nicotiana tabacum]
gi|8096269|dbj|BAA95789
Length = 460
Score = 27.1 bits (57), Expect = 7.9
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +1
Query: 25 IY*FRMACSV---SDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKI 165
I+ F+ C+V D+ + P+V++D EG N L DV+ NEKI
Sbjct: 92 IFAFQTVCAVLFLGDSTKSEKTPEVSSDS----EGNNLVLLEDVEMNEKI 137
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 27.1 bits (57), Expect = 7.9
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +3
Query: 249 AEHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNSLPTKD 410
+E E EK KDA E + NK L+ EN D K + E +++ +KD
Sbjct: 782 SEKVEKGEKKE--SKDAKSVETKDNKKLSSTENRDEAKERSGEDNKEDKEESKD 833
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,271,706
Number of Sequences: 28952
Number of extensions: 238128
Number of successful extensions: 860
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 818
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 859
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 984125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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