BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20293 (563 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B) 110 5e-25 At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S ... 107 7e-24 At4g35380.1 68417.m05026 guanine nucleotide exchange family prot... 27 6.5 At3g16810.1 68416.m02147 pumilio/Puf RNA-binding domain-containi... 27 6.5 At4g02110.1 68417.m00282 BRCT domain-containing protein contains... 27 8.7 At1g30330.1 68414.m03709 auxin-responsive factor (ARF6) identica... 27 8.7 >At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B) Length = 146 Score = 110 bits (265), Expect = 5e-25 Identities = 51/66 (77%), Positives = 60/66 (90%) Frame = +3 Query: 57 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 236 MKFN +V+SSRRKNRK HF+APS +RRVLMSSPLSK+LR K NV+SMPIRKDDEVQVVRG Sbjct: 1 MKFNPRVSSSRRKNRKAHFTAPSSVRRVLMSSPLSKDLRNKHNVRSMPIRKDDEVQVVRG 60 Query: 237 HYKGQQ 254 +KG++ Sbjct: 61 TFKGRE 66 Score = 87.0 bits (206), Expect = 8e-18 Identities = 40/68 (58%), Positives = 56/68 (82%) Frame = +2 Query: 257 GKVMQVYRKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRL 436 GKVMQVYR+K+V++IERI REK NG+T VG++ S +I KL+++KDRK++L+R+A GR Sbjct: 67 GKVMQVYRRKWVIHIERITREKVNGSTVNVGVNASNVMITKLRLDKDRKSLLERKANGR- 125 Query: 437 AALGKDKG 460 AA K+KG Sbjct: 126 AAADKEKG 133 >At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S RIBOSOMAL PROTEIN L26, Brassica rapa, EMBL:BRD495 Length = 146 Score = 107 bits (256), Expect = 7e-24 Identities = 49/66 (74%), Positives = 59/66 (89%) Frame = +3 Query: 57 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 236 MK+N +VTSSRRKNRK HF+A S RRV+MSSPLS +LRQK+NV+SMPIRKDDEVQ+VRG Sbjct: 1 MKYNPRVTSSRRKNRKAHFTASSSERRVIMSSPLSTDLRQKYNVRSMPIRKDDEVQIVRG 60 Query: 237 HYKGQQ 254 YKG++ Sbjct: 61 TYKGRE 66 Score = 93.5 bits (222), Expect = 9e-20 Identities = 44/68 (64%), Positives = 57/68 (83%) Frame = +2 Query: 257 GKVMQVYRKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRL 436 GKV+QVYR+K+V++IERI REK NG T VGI PSK VI KL+++KDRK++L+R+AKGR Sbjct: 67 GKVVQVYRRKWVIHIERITREKVNGTTVNVGIQPSKVVITKLRLDKDRKSLLERKAKGR- 125 Query: 437 AALGKDKG 460 AA K+KG Sbjct: 126 AAADKEKG 133 >At4g35380.1 68417.m05026 guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor [Homo sapiens] GI:5456754; contains Pfam profile PF01369: Sec7 domain Length = 1706 Score = 27.5 bits (58), Expect = 6.5 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 82 PQEGKTGRGISVLLHI 129 P +G TG GISVLLH+ Sbjct: 1408 PFQGSTGSGISVLLHL 1423 >At3g16810.1 68416.m02147 pumilio/Puf RNA-binding domain-containing protein contains Pfam profile:PF00806 Pumilio-family RNA binding domains Length = 641 Score = 27.5 bits (58), Expect = 6.5 Identities = 21/92 (22%), Positives = 40/92 (43%), Gaps = 6/92 (6%) Frame = +3 Query: 42 AKSDRMKFNKQVTSSRRKNRKRHFSAPSHIRRV---LMSSPLSKELRQKFNVKSMPIRKD 212 +K +R K++T +R+K RK H++ + + + + KE R K +++ K Sbjct: 62 SKKERRVQAKELTEARKKKRKPHYNLEQELVSLWEKMRRRNIGKEDRSKLISEAIRKMKG 121 Query: 213 DEVQVVRGHYKG---QQLAK*CRCIXKSLLYT 299 ++ H Q K C K +L+T Sbjct: 122 KVPEIAVSHVSSRVLQTCVKFCSQAEKDVLFT 153 >At4g02110.1 68417.m00282 BRCT domain-containing protein contains Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain Length = 1293 Score = 27.1 bits (57), Expect = 8.7 Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 3/64 (4%) Frame = +3 Query: 36 VAAKSDRMKFNKQVT---SSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIR 206 VA K+ +K T +S ++ +RH S ++ R+ L SP + L ++ + ++ +R Sbjct: 258 VANKTPEQGMSKMETDGSTSINRSIRRHSSLATYSRKTLQRSPETDTLGKESSGQNRSLR 317 Query: 207 KDDE 218 DD+ Sbjct: 318 MDDK 321 >At1g30330.1 68414.m03709 auxin-responsive factor (ARF6) identical to ARF6 [Arabidopsis thaliana] GI:4102600 (Science 276 (5320), 1865-1868 (1997)) Length = 933 Score = 27.1 bits (57), Expect = 8.7 Identities = 11/51 (21%), Positives = 25/51 (49%) Frame = -1 Query: 167 FLRQGRGHQHSPYM*RSTEMPLPVFPS*GSHLLVELHSVALRCNAKTPRLF 15 + QG Q + + + +P +PS L+ +LH+V + + +T ++ Sbjct: 44 YFPQGHSEQVAASTNKEVDAHIPNYPSLHPQLICQLHNVTMHADVETDEVY 94 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,238,782 Number of Sequences: 28952 Number of extensions: 231854 Number of successful extensions: 653 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 638 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 653 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1082538160 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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