BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20290 (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A2.08c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.20 SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr... 29 0.34 SPBC577.05c |rec27|mug41|meiotic recombination protein Rec27|Sch... 26 4.2 SPAC22H10.08 |||DUF2009 protein|Schizosaccharomyces pombe|chr 1|... 26 4.2 SPAC1952.07 |rad1||checkpoint clamp complex protein Rad1|Schizos... 26 4.2 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 25 5.6 SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 25 7.3 SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 25 9.7 SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 25 9.7 SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|ch... 25 9.7 >SPAC17A2.08c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 30.3 bits (65), Expect = 0.20 Identities = 24/82 (29%), Positives = 43/82 (52%), Gaps = 3/82 (3%) Frame = +2 Query: 215 LKQISSE*SLLFVLNKIND--IHNFYTKVIENEKKLLCYHTSKDQVRN-SVQTANEMQDV 385 L Q E LL ++I D F V E+EK YH S + +R S+Q A + +++ Sbjct: 103 LAQNHKEEKLLQTEDEIQDEGYSGFENYVEESEKLQEIYHHSSESLRTRSIQMAVDQKNM 162 Query: 386 NIEYCNENIFCETESNDNTEMQ 451 +E +E+I +S ++T+++ Sbjct: 163 EMELDDEDIAEPIQSWEHTQIK 184 >SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 593 Score = 29.5 bits (63), Expect = 0.34 Identities = 18/63 (28%), Positives = 34/63 (53%), Gaps = 1/63 (1%) Frame = +2 Query: 266 NDIHNFYTKVIENEKKLLCYHTSKDQVRNSVQTANEMQDVNIE-YCNENIFCETESNDNT 442 +++ NF+TKV+E+ K L D + +++T ++ I+ N N F E D+T Sbjct: 150 SELQNFFTKVVEDNIKALIKPQYVDHIPRAIKTGDKTVSGLIKARANNNNF--EEVMDHT 207 Query: 443 EMQ 451 ++Q Sbjct: 208 DLQ 210 >SPBC577.05c |rec27|mug41|meiotic recombination protein Rec27|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 25.8 bits (54), Expect = 4.2 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 3/50 (6%) Frame = +2 Query: 254 LNKINDIHNFYTKVIENEKKLLCYHTSKDQVR---NSVQTANEMQDVNIE 394 + K + I + Y IENEKK S+ Q + +Q AN+++ +N E Sbjct: 43 VKKYDQIESEYKTNIENEKKAFIKDVSQVQQKIKEFEIQKANQIKQLNEE 92 >SPAC22H10.08 |||DUF2009 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 25.8 bits (54), Expect = 4.2 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = -1 Query: 461 LALTASQYYHCSQFHKKYFHYSIQYLHLAFHLLFELNS 348 LA+ A H K +HY +Q LHL ++ E+ S Sbjct: 279 LAIDAINGSRLKHSHYKQYHYVVQSLHLWLLIMGEIFS 316 >SPAC1952.07 |rad1||checkpoint clamp complex protein Rad1|Schizosaccharomyces pombe|chr 1|||Manual Length = 323 Score = 25.8 bits (54), Expect = 4.2 Identities = 18/93 (19%), Positives = 41/93 (44%) Frame = +2 Query: 251 VLNKINDIHNFYTKVIENEKKLLCYHTSKDQVRNSVQTANEMQDVNIEYCNENIFCETES 430 ++ K N +++ ++ N + L HTS + ++ + IEY NE E+ Sbjct: 161 IIMKSNWLYDALVELDNNMGENLIIHTSSQKSTFLLRCVGALSTTEIEYPNEKSVLESFE 220 Query: 431 NDNTEMQLKQENLLSTTIYPNSFQITRKISIFI 529 D+ + +L+ + + Q+ K+++ I Sbjct: 221 TDSENTYSYRFSLIRHAL--KALQVGSKVNLRI 251 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.4 bits (53), Expect = 5.6 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +2 Query: 296 IENEKKLLCYHTSKDQVRNS 355 +EN K LC + S D+VRNS Sbjct: 1200 LENTVKYLCEYDSIDKVRNS 1219 >SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1|||Manual Length = 113 Score = 25.0 bits (52), Expect = 7.3 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = +2 Query: 278 NFYTKVIENEKKLLCYHTSKDQV 346 N Y K+++NE + YH+ D V Sbjct: 11 NVYGKLVDNETRCFHYHSKADVV 33 >SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 1052 Score = 24.6 bits (51), Expect = 9.7 Identities = 21/104 (20%), Positives = 49/104 (47%), Gaps = 1/104 (0%) Frame = +2 Query: 176 FNENIKMLLTKDYLKQISSE*SLLFVLNKINDIHNFYTKV-IENEKKLLCYHTSKDQVRN 352 + + +L + ++Q+ +E ++ K D+ + + V ++ E+++ +H +KDQVRN Sbjct: 440 YQQQQSLLQLESNIQQLENEKKAAKLIEK--DLLSQISHVKLQFEQQIKAHHYAKDQVRN 497 Query: 353 SVQTANEMQDVNIEYCNENIFCETESNDNTEMQLKQENLLSTTI 484 +EM + E + +++ +KQ N L + Sbjct: 498 ---LNDEMVALRAELEVVKAMNSQPDDPDSDYAIKQNNALQNRL 538 >SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 592 Score = 24.6 bits (51), Expect = 9.7 Identities = 12/44 (27%), Positives = 23/44 (52%) Frame = +2 Query: 146 YDDYSQET*CFNENIKMLLTKDYLKQISSE*SLLFVLNKINDIH 277 +D ++ET F EN+ + +D L + E ++ +L+ N H Sbjct: 198 FDIKNEETFKFRENVALETLEDLLHWMIHENGVIGILDATNSTH 241 >SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 401 Score = 24.6 bits (51), Expect = 9.7 Identities = 18/69 (26%), Positives = 33/69 (47%), Gaps = 3/69 (4%) Frame = +2 Query: 263 INDIHNFYTKVIENEKKLLCYHTSKDQVRNSVQTANEMQD---VNIEYCNENIFCETESN 433 I D+ ++E E +LL T Q + +EMQ+ V + C E + ETE Sbjct: 146 ITDLRQQNASLVE-ENQLLS--TQSKQWERRARDEHEMQESLAVRLADCEEQLARETERQ 202 Query: 434 DNTEMQLKQ 460 + E+++++ Sbjct: 203 EQYEVEIQR 211 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,055,019 Number of Sequences: 5004 Number of extensions: 40542 Number of successful extensions: 135 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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