Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20288
(350 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase / 10-f... 93 5e-20
At4g15100.1 68417.m02321 serine carboxypeptidase S10 family prot... 30 0.38
At1g11080.1 68414.m01269 serine carboxypeptidase S10 family prot... 30 0.50
At1g61130.1 68414.m06887 serine carboxypeptidase S10 family prot... 29 0.66
At5g59670.1 68418.m07481 leucine-rich repeat protein kinase, put... 27 2.7
At3g52140.1 68416.m05723 tetratricopeptide repeat (TPR)-containi... 27 2.7
At2g46580.1 68415.m05810 pyridoxine 5'-phosphate oxidase-related... 27 2.7
At2g32930.1 68415.m04037 zinc finger (CCCH-type) family protein ... 27 3.5
At3g58070.1 68416.m06473 zinc finger (C2H2 type) family protein ... 24 5.7
At4g20320.1 68417.m02967 CTP synthase, putative / UTP--ammonia l... 26 6.1
At3g54590.1 68416.m06040 proline-rich extensin-like family prote... 26 6.1
At3g54580.1 68416.m06039 proline-rich extensin-like family prote... 26 6.1
At1g29730.1 68414.m03634 leucine-rich repeat transmembrane prote... 26 6.1
At5g08260.1 68418.m00971 serine carboxypeptidase S10 family prot... 26 8.1
At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMP... 26 8.1
At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMP... 26 8.1
>At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase /
10-formyltetrahydrofolate synthetase (THFS) identical to
10-formyltetrahydrofolate synthetase (Arabidopsis
thaliana) GI:5921663
Length = 634
Score = 93.1 bits (221), Expect = 5e-20
Identities = 43/83 (51%), Positives = 56/83 (67%)
Frame = +2
Query: 5 ITPPPSDIVIARSQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDRLRNQRTGKYI 184
++P P+DI IA S +P I E+A ++ + P YG+ KAK+ LS D L+ Q G Y+
Sbjct: 11 VSPVPADIDIANSVEPLHISEIAKDLNINPLHYDLYGKYKAKVLLSAFDELQGQEDGYYV 70
Query: 185 VVAGITPTPLGEGKSTTLIGLVQ 253
VV GITPTPLGEGKSTT +GL Q
Sbjct: 71 VVGGITPTPLGEGKSTTTVGLCQ 93
Score = 50.8 bits (116), Expect = 2e-07
Identities = 21/31 (67%), Positives = 24/31 (77%)
Frame = +1
Query: 256 LTAHRGKNAFACMRQPSQGPTFGVKGGAAGG 348
L A+ K C+RQPSQGPTFG+KGGAAGG
Sbjct: 95 LGAYLDKKVVTCLRQPSQGPTFGIKGGAAGG 125
>At4g15100.1 68417.m02321 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II chains A and B
(SP:P08819) (EC 3.4.16.6) [Triticum aestivum (Wheat)]
Length = 407
Score = 30.3 bits (65), Expect = 0.38
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +1
Query: 130 DFTVRSRQITQPTHREIHRSCWYNSDTTWGRQK 228
D+ I+ THR I+R C ++SD W K
Sbjct: 161 DYAWSHAVISDETHRNINRLCNFSSDDVWNNDK 193
>At1g11080.1 68414.m01269 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II chains A and B
(SP:P08819) (EC 3.4.16.6) [Triticum aestivum (Wheat)]
Length = 492
Score = 29.9 bits (64), Expect = 0.50
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +1
Query: 130 DFTVRSRQITQPTHREIHRSCWYNSDTTWGRQK 228
D+ I+ THR I R+C ++SD TW +
Sbjct: 250 DYAWSHAVISDETHRIITRTCNFSSDNTWSNDE 282
>At1g61130.1 68414.m06887 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II-3 precursor
(SP:P52711) (CP-MII.3. [Hordeum vulgare]
Length = 463
Score = 29.5 bits (63), Expect = 0.66
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +1
Query: 130 DFTVRSRQITQPTHREIHRSCWYNSDTTW 216
D+ ++ T+R I +SC ++SDTTW
Sbjct: 229 DYAWNHAVVSDETYRVIKQSCNFSSDTTW 257
>At5g59670.1 68418.m07481 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 868
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = -1
Query: 119 YVHIETPRSDTDRSLLPALRCL*ASENELSRCHW 18
Y I+ PRS+TD S + A++ + AS LSR +W
Sbjct: 352 YTVIQFPRSETDESDVVAMKNISASYG-LSRINW 384
>At3g52140.1 68416.m05723 tetratricopeptide repeat (TPR)-containing
protein contains Pfam profile PF00515: TPR Domain
Length = 1403
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +1
Query: 112 WTY*GQDFTVRSRQITQPTHREIHRSCWYNSDTTWGRQKHHSNRAGARLTAHR 270
+T+ + F++ +Q+T P HRE+ C Y + + H + AGA + H+
Sbjct: 1041 YTFFSEAFSIL-QQVTGPMHREVANCCRYLAMVLY----HAGDMAGAIMQQHK 1088
>At2g46580.1 68415.m05810 pyridoxine 5'-phosphate oxidase-related
similar to pyridoxine 5'-phosphate oxidase (GI:3237304)
[Rattus norvegicus]; similar to pyridoxamine
5'-phosphate oxidase (GI:22294052) [Thermosynechococcus
elongatus BP-1]
Length = 198
Score = 27.5 bits (58), Expect = 2.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +1
Query: 139 VRSRQITQPTHREIHRSCWYNSDT 210
+RSR+I + H CWY SDT
Sbjct: 62 LRSRKIEELKHCPFSEMCWYFSDT 85
>At2g32930.1 68415.m04037 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 453
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +2
Query: 2 PITPPPSDIVIARSQKPKDIGELAAEIGLC 91
P+T P I+ R Q P+ +G+ E G C
Sbjct: 70 PLTNLPQGIIYYRDQLPERVGQPDCETGAC 99
>At3g58070.1 68416.m06473 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 253
Score = 24.2 bits (50), Expect(2) = 5.7
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +1
Query: 58 HRRAGSRDRSVSERGVSIWTY 120
H+ A R+R +++RGVS + Y
Sbjct: 109 HQNAHKRERQLAKRGVSSYFY 129
Score = 20.6 bits (41), Expect(2) = 5.7
Identities = 11/40 (27%), Positives = 18/40 (45%)
Frame = +1
Query: 187 SCWYNSDTTWGRQKHHSNRAGARLTAHRGKNAFACMRQPS 306
S +Y+ D +H+ + LTA R F+ +PS
Sbjct: 126 SYFYHPDNNPYSYRHYPSWTNGPLTAARSYGGFSSGPKPS 165
>At4g20320.1 68417.m02967 CTP synthase, putative / UTP--ammonia
ligase, putative similar to SP|P17812 CTP synthase (EC
6.3.4.2) (UTP--ammonia ligase) {Homo sapiens}; contains
Pfam profile PF00117: glutamine amidotransferase class-I
Length = 563
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 176 KYIVVAGITPTPLGEGKSTTLIGLV 250
KY+VV+G + LG+G + + IGL+
Sbjct: 2 KYVVVSGGVVSGLGKGVTASSIGLI 26
>At3g54590.1 68416.m06040 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 743
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/42 (38%), Positives = 16/42 (38%)
Frame = -3
Query: 297 PHACKCVLPAMCCQPCTSPIRVVLLPSPSGVGVIPATTMYFP 172
PH C C P C P SP V P P V P Y P
Sbjct: 665 PHVCVCPPPPPCYSP--SPKVVYKSPPPPYVYNSPPPPYYSP 704
>At3g54580.1 68416.m06039 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 951
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/42 (38%), Positives = 16/42 (38%)
Frame = -3
Query: 297 PHACKCVLPAMCCQPCTSPIRVVLLPSPSGVGVIPATTMYFP 172
PH C C P C P SP V P P V P Y P
Sbjct: 690 PHVCVCPPPPPCYSP--SPKVVYKSPPPPYVYSSPPPPHYSP 729
>At1g29730.1 68414.m03634 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam domains, PF00560: Leucine
Rich Repeat and PF00069: Protein kinase domain
Length = 940
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 62 GELAAEIGLCPSEVSQYGRTKAKIS 136
G L + + +CPS S+ G K KIS
Sbjct: 563 GSLISAVSVCPSSESECGGMKKKIS 587
>At5g08260.1 68418.m00971 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II chains A and B
(SP:P08819) (EC 3.4.16.6) [Triticum aestivum (Wheat)];
carboxypeptidase D - Triticum aestivum, PIR:A29639
Length = 480
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +1
Query: 154 ITQPTHREIHRSCWYNSDTTWGRQKHHSNRAG 249
I+ H IH SC + DTT ++ ++N G
Sbjct: 245 ISDEVHTSIHGSCSFEEDTTNKTEQCYNNFKG 276
>At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMPA4)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 538
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/60 (26%), Positives = 26/60 (43%), Gaps = 5/60 (8%)
Frame = +2
Query: 41 SQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDRLRNQRTG-----KYIVVAGITP 205
S P D + E G+CP V G + + L + N TG ++I+ +G+ P
Sbjct: 277 SDGPNDKIQAVIEAGVCPRLVELLGHQSPTVLIPALRTVGNIVTGDDSQTQFIIESGVLP 336
>At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMPA4)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 538
Score = 25.8 bits (54), Expect = 8.1
Identities = 16/60 (26%), Positives = 26/60 (43%), Gaps = 5/60 (8%)
Frame = +2
Query: 41 SQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDRLRNQRTG-----KYIVVAGITP 205
S P D + E G+CP V G + + L + N TG ++I+ +G+ P
Sbjct: 277 SDGPNDKIQAVIEAGVCPRLVELLGHQSPTVLIPALRTVGNIVTGDDSQTQFIIESGVLP 336
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,589,412
Number of Sequences: 28952
Number of extensions: 180902
Number of successful extensions: 558
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 543
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 558
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 439384704
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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