BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20283 (468 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g18670.1 68414.m02330 protein kinase family protein contains ... 30 0.90 At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, p... 29 1.6 At2g34680.1 68415.m04260 leucine-rich repeat family protein cont... 28 2.7 At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)... 27 4.8 At5g42770.1 68418.m05209 Maf family protein contains Pfam domain... 27 8.4 At1g68000.1 68414.m07768 CDP-diacylglycerol--inositol 3-phosphat... 27 8.4 >At1g18670.1 68414.m02330 protein kinase family protein contains Protein kinases ATP-binding region signature, PROSITE:PS00107 and Serine/Threonine protein kinases active-site signature, PROSITE:PS00108 Length = 662 Score = 29.9 bits (64), Expect = 0.90 Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 1/54 (1%) Frame = +3 Query: 225 RRTVFCIRSYNETLAENNKLKITGSDFKFSSDLETDEVTS-KVKEFYFGENTIS 383 R+ C+RS+N +L+ + + G FS + + D + K KE GE T S Sbjct: 552 RKDDICVRSHNRSLSRGHIPNLLGPSPAFSENTDVDSKNNEKEKEEKHGERTDS 605 >At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, putative / HSP70, mitochondrial, putative strong similarity to SP|Q01899 Heat shock 70 kDa protein, mitochondrial precursor {Phaseolus vulgaris} Length = 682 Score = 29.1 bits (62), Expect = 1.6 Identities = 16/73 (21%), Positives = 33/73 (45%) Frame = +1 Query: 34 TPYKELINVTVGKPLGQLLISNLLHLPCIEKVFPDSEPVITDLPINLLKNAPKNISVIYG 213 T KE +GK + + +++ + ++ I L + + N P ++ YG Sbjct: 171 TKMKETAEAYLGKSINKAVVTVPAYFNDAQRQATKDAGKIAGLDVQRIINEPTAAALSYG 230 Query: 214 SNDKEGLFFVSEV 252 N+KEG+ V ++ Sbjct: 231 MNNKEGVIAVFDL 243 >At2g34680.1 68415.m04260 leucine-rich repeat family protein contains leucine rich repeat (LRR) domains, Pfam:PF00560; identical to cDNA hypothetical protein (AIR9) mRNA, partial cds GI:3695020 Length = 1661 Score = 28.3 bits (60), Expect = 2.7 Identities = 12/47 (25%), Positives = 26/47 (55%) Frame = -3 Query: 295 PVIFNLLFSANVSL*LLIQKTVLLYHSSRRSPKCSSVHFLVNL*VGQ 155 P++ ++ S+++ + + VL+ + R P C S+ FL ++ GQ Sbjct: 990 PIVAGIILSSHLLSSFAVSEGVLISMNERGHPNCQSLEFLGSMIEGQ 1036 >At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5) identical to heat shock protein 70 [Arabidopsis thaliana] GI:6746590 Length = 682 Score = 27.5 bits (58), Expect = 4.8 Identities = 17/76 (22%), Positives = 33/76 (43%) Frame = +1 Query: 25 FAKTPYKELINVTVGKPLGQLLISNLLHLPCIEKVFPDSEPVITDLPINLLKNAPKNISV 204 F T KE +GK + + +++ + ++ I L + + N P ++ Sbjct: 173 FILTKMKETAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAAL 232 Query: 205 IYGSNDKEGLFFVSEV 252 YG +KEGL V ++ Sbjct: 233 SYGMTNKEGLIAVFDL 248 >At5g42770.1 68418.m05209 Maf family protein contains Pfam domain PF02545: Maf-like protein Length = 233 Score = 26.6 bits (56), Expect = 8.4 Identities = 22/68 (32%), Positives = 35/68 (51%), Gaps = 2/68 (2%) Frame = +1 Query: 1 DPQELY--DIFAKTPYKELINVTVGKPLGQLLISNLLHLPCIEKVFPDSEPVITDLPINL 174 D E+Y +I +T K + V K G LLI + L LPC+++V ++ V+ LP L Sbjct: 163 DRVEIYFNEIPEETIEKLIEEGMVLKVAGALLIEHPLILPCVKEVVGTTDSVM-GLPKEL 221 Query: 175 LKNAPKNI 198 + K + Sbjct: 222 TEKLIKEV 229 >At1g68000.1 68414.m07768 CDP-diacylglycerol--inositol 3-phosphatidyltransferase / phosphatidylinositol synthase (PIS1) identical to phosphatidylinositol synthase (PIS1) GB:AJ000539 [gi:3367632] Length = 227 Score = 26.6 bits (56), Expect = 8.4 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -2 Query: 392 CFPAYGIFSKIKFFNFRCDLISF*I*RKF 306 CF +FS + FF+F CD + + R+F Sbjct: 37 CFSNKPLFSVLYFFSFCCDAVDGWVARRF 65 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,707,692 Number of Sequences: 28952 Number of extensions: 189107 Number of successful extensions: 537 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 524 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 537 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 791932800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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