BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20270
(507 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g05615.1 68414.m00582 hypothetical protein 28 4.2
At5g44360.1 68418.m05432 FAD-binding domain-containing protein s... 27 7.3
At3g29770.1 68416.m03774 hydrolase, alpha/beta fold family prote... 27 7.3
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 27 9.6
>At1g05615.1 68414.m00582 hypothetical protein
Length = 172
Score = 27.9 bits (59), Expect = 4.2
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 130 TSPLIVCLSKIYRVLDVCLFNELHEVRFFRLH 225
TSPL + +S Y V+ C E +E+R + H
Sbjct: 123 TSPLYILVSGRYNVVKGCRLKEGNEIRIWSFH 154
>At5g44360.1 68418.m05432 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 532
Score = 27.1 bits (57), Expect = 7.3
Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = -1
Query: 84 YIYLTVFVTTHKPHLMITPREKT-FRRSI 1
Y +LT+ T+ KP L++TPR T +RS+
Sbjct: 72 YRFLTLNFTSQKPILIVTPRTDTEIQRSL 100
>At3g29770.1 68416.m03774 hydrolase, alpha/beta fold family protein
similar to SP|Q40708 PIR7A protein {Oryza sativa},
polyneuridine aldehyde esterase [Rauvolfia serpentina]
GI:6651393; contains Pfam profile: PF00561 alpha/beta
hydrolase fold
Length = 390
Score = 27.1 bits (57), Expect = 7.3
Identities = 17/56 (30%), Positives = 29/56 (51%)
Frame = -2
Query: 314 SCAVVKSRKHSQNAYTLFTYQTNSYRVQWECNLKNRTSWSSLNKHTSSTRYILERH 147
+ A V R+ + +L ++ S R Q C+ KN+ SS ++ SST +L+ H
Sbjct: 67 AAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQLPRSSSSRSRSSTDPLLQPH 122
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 26.6 bits (56), Expect = 9.6
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = -2
Query: 251 TNSYRVQWECNLKNRTSWSSLNKHTSSTRYILERHTISG 135
+ Y W+ L N SW L S+ R+ LE + G
Sbjct: 444 SRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDG 482
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,456,194
Number of Sequences: 28952
Number of extensions: 201393
Number of successful extensions: 342
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 340
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 342
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 908059136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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