BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20270 (507 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g05615.1 68414.m00582 hypothetical protein 28 4.2 At5g44360.1 68418.m05432 FAD-binding domain-containing protein s... 27 7.3 At3g29770.1 68416.m03774 hydrolase, alpha/beta fold family prote... 27 7.3 At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 27 9.6 >At1g05615.1 68414.m00582 hypothetical protein Length = 172 Score = 27.9 bits (59), Expect = 4.2 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 130 TSPLIVCLSKIYRVLDVCLFNELHEVRFFRLH 225 TSPL + +S Y V+ C E +E+R + H Sbjct: 123 TSPLYILVSGRYNVVKGCRLKEGNEIRIWSFH 154 >At5g44360.1 68418.m05432 FAD-binding domain-containing protein similar to SP|P30986 reticuline oxidase precursor (Berberine-bridge-forming enzyme) (BBE) (Tetrahydroprotoberberine synthase) [Eschscholzia californica]; contains PF01565 FAD binding domain Length = 532 Score = 27.1 bits (57), Expect = 7.3 Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 1/29 (3%) Frame = -1 Query: 84 YIYLTVFVTTHKPHLMITPREKT-FRRSI 1 Y +LT+ T+ KP L++TPR T +RS+ Sbjct: 72 YRFLTLNFTSQKPILIVTPRTDTEIQRSL 100 >At3g29770.1 68416.m03774 hydrolase, alpha/beta fold family protein similar to SP|Q40708 PIR7A protein {Oryza sativa}, polyneuridine aldehyde esterase [Rauvolfia serpentina] GI:6651393; contains Pfam profile: PF00561 alpha/beta hydrolase fold Length = 390 Score = 27.1 bits (57), Expect = 7.3 Identities = 17/56 (30%), Positives = 29/56 (51%) Frame = -2 Query: 314 SCAVVKSRKHSQNAYTLFTYQTNSYRVQWECNLKNRTSWSSLNKHTSSTRYILERH 147 + A V R+ + +L ++ S R Q C+ KN+ SS ++ SST +L+ H Sbjct: 67 AAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQLPRSSSSRSRSSTDPLLQPH 122 >At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch branching enzyme class II (SBE2-1) nearly identical to starch branching enzyme class II [Arabidopsis thaliana] GI:619939 Length = 858 Score = 26.6 bits (56), Expect = 9.6 Identities = 12/39 (30%), Positives = 17/39 (43%) Frame = -2 Query: 251 TNSYRVQWECNLKNRTSWSSLNKHTSSTRYILERHTISG 135 + Y W+ L N SW L S+ R+ LE + G Sbjct: 444 SRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDG 482 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,456,194 Number of Sequences: 28952 Number of extensions: 201393 Number of successful extensions: 342 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 340 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 342 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 908059136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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