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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20270
         (507 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g05615.1 68414.m00582 hypothetical protein                          28   4.2  
At5g44360.1 68418.m05432 FAD-binding domain-containing protein s...    27   7.3  
At3g29770.1 68416.m03774 hydrolase, alpha/beta fold family prote...    27   7.3  
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta...    27   9.6  

>At1g05615.1 68414.m00582 hypothetical protein
          Length = 172

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +1

Query: 130 TSPLIVCLSKIYRVLDVCLFNELHEVRFFRLH 225
           TSPL + +S  Y V+  C   E +E+R +  H
Sbjct: 123 TSPLYILVSGRYNVVKGCRLKEGNEIRIWSFH 154


>At5g44360.1 68418.m05432 FAD-binding domain-containing protein
           similar to  SP|P30986 reticuline oxidase precursor
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase) [Eschscholzia
           californica]; contains PF01565 FAD binding domain
          Length = 532

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
 Frame = -1

Query: 84  YIYLTVFVTTHKPHLMITPREKT-FRRSI 1
           Y +LT+  T+ KP L++TPR  T  +RS+
Sbjct: 72  YRFLTLNFTSQKPILIVTPRTDTEIQRSL 100


>At3g29770.1 68416.m03774 hydrolase, alpha/beta fold family protein
           similar to SP|Q40708 PIR7A protein {Oryza sativa},
           polyneuridine aldehyde esterase [Rauvolfia serpentina]
           GI:6651393; contains Pfam profile: PF00561 alpha/beta
           hydrolase fold
          Length = 390

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 17/56 (30%), Positives = 29/56 (51%)
 Frame = -2

Query: 314 SCAVVKSRKHSQNAYTLFTYQTNSYRVQWECNLKNRTSWSSLNKHTSSTRYILERH 147
           + A V  R+ +    +L   ++ S R Q  C+ KN+   SS ++  SST  +L+ H
Sbjct: 67  AAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQLPRSSSSRSRSSTDPLLQPH 122


>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-1) nearly identical to
           starch branching enzyme class II [Arabidopsis thaliana]
           GI:619939
          Length = 858

 Score = 26.6 bits (56), Expect = 9.6
 Identities = 12/39 (30%), Positives = 17/39 (43%)
 Frame = -2

Query: 251 TNSYRVQWECNLKNRTSWSSLNKHTSSTRYILERHTISG 135
           +  Y   W+  L N  SW  L    S+ R+ LE +   G
Sbjct: 444 SRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDG 482


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,456,194
Number of Sequences: 28952
Number of extensions: 201393
Number of successful extensions: 342
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 340
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 342
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 908059136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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