BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20265 (490 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16E9.18 ||SPBC1E8.01|phosphatidylserine decarboxylase|Schizo... 27 1.1 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 27 2.0 SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 25 4.6 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 4.6 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 4.6 SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|ch... 25 6.1 SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.1 >SPBC16E9.18 ||SPBC1E8.01|phosphatidylserine decarboxylase|Schizosaccharomyces pombe|chr 2|||Manual Length = 437 Score = 27.5 bits (58), Expect = 1.1 Identities = 22/63 (34%), Positives = 32/63 (50%), Gaps = 1/63 (1%) Frame = -2 Query: 267 TACSILHGVPTSVNDQVVNYILDDGALALVLVFQALTD-SAVVVTGEDAVVQFLLEFFVR 91 ++ SILHG P D+V N+ L +VLVF+A D +V G+ V L V Sbjct: 378 SSSSILHGHPLLRGDEVGNFELGS---TVVLVFEAPADFEFLVKQGQKVRVGLPLGRVVP 434 Query: 90 TAH 82 ++H Sbjct: 435 SSH 437 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 26.6 bits (56), Expect = 2.0 Identities = 13/58 (22%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Frame = +3 Query: 315 FRLIMAGNFVKLIYRNYNLXLKLGPTLDPANERLAYGD-GKEKNSDLISWKVHYLVGE 485 +R+ + ++ + + N + + + AN R+ G G E + ++I+WK+ +GE Sbjct: 316 YRISIRADYPHKLSSSLNFRIPVPTNVVKANPRVNRGKAGYEPSENIINWKIPRFLGE 373 >SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 4.6 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 94 NKELEEKLYNSILTGDYDSAVRQSLEYENQGKGSII 201 N+++ K+ L G Y +A ++ QGKGS+I Sbjct: 109 NEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLI 144 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.4 bits (53), Expect = 4.6 Identities = 16/50 (32%), Positives = 25/50 (50%) Frame = +1 Query: 178 NQGKGSIIQNVVNNLIIDGSRNTMEYATSCGSATDSTLSESTSPITLDSS 327 +Q +GS+ Q+ +GS +T+ +T G ST + STS T S Sbjct: 103 SQNQGSLAQS--EQFDFEGSDSTLAASTISGGIYSSTSASSTSSSTATPS 150 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.4 bits (53), Expect = 4.6 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = +1 Query: 94 NKELEEKLYNSILTGDYDSAVRQSLE 171 + +LE+ + ++LTGD SAV+ LE Sbjct: 551 DSDLEKNITEALLTGDVLSAVKACLE 576 >SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 257 Score = 25.0 bits (52), Expect = 6.1 Identities = 14/45 (31%), Positives = 20/45 (44%) Frame = -2 Query: 255 ILHGVPTSVNDQVVNYILDDGALALVLVFQALTDSAVVVTGEDAV 121 +LH P S D + I DGALA + + SA+ D + Sbjct: 121 LLHLYPKSSIDVYIQVIESDGALATLAAAISCASSAIADANIDCI 165 >SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 300 Score = 24.6 bits (51), Expect = 8.1 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = -2 Query: 180 VLVFQALTDSAVVVTGEDAVVQFLLEFFV 94 + F +T V+V EDAVV+F+L V Sbjct: 178 IYFFLGVTVQYVMVLPEDAVVEFVLTILV 206 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.130 0.371 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,828,733 Number of Sequences: 5004 Number of extensions: 33633 Number of successful extensions: 91 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 90 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 91 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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