BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20265
(490 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g59210.2 68418.m07421 myosin heavy chain-related contains wea... 31 0.42
At5g59210.1 68418.m07420 myosin heavy chain-related contains wea... 31 0.42
At4g11270.1 68417.m01823 transducin family protein / WD-40 repea... 30 0.96
At2g15490.1 68415.m01772 UDP-glucoronosyl/UDP-glucosyl transfera... 30 0.96
At4g13310.2 68417.m02080 cytochrome P450 71A20, putative (CYP71A... 29 1.3
At4g13310.1 68417.m02081 cytochrome P450 71A20, putative (CYP71A... 29 1.3
At4g22200.1 68417.m03209 potassium channel protein 2 (AKT2) (AKT... 27 5.1
At5g16270.1 68418.m01900 Rad21/Rec8-like family protein weak sim... 27 6.8
At1g04120.1 68414.m00401 ABC transporter family protein Strong s... 27 6.8
>At5g59210.2 68418.m07421 myosin heavy chain-related contains weak
similarity to Myosin heavy chain, gizzard smooth muscle
(Swiss-Prot:P10587) [Gallus gallus]
Length = 433
Score = 31.1 bits (67), Expect = 0.42
Identities = 23/73 (31%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
Frame = +1
Query: 109 EKLY--NSILTGDYDSAVRQSLEYENQGKGSIIQNVVNNLIIDGSRNTMEYATSCG-SAT 279
EKL+ NS L+ Y ++ S ++ENQ K + QNV ++D R + S G S
Sbjct: 298 EKLFEENSNLSASYQESINISNQWENQVKECLKQNVELREVLDKLRTEQAGSFSRGPSEF 357
Query: 280 DSTLSESTSPITL 318
++ S T ++L
Sbjct: 358 EANGSHGTDTLSL 370
>At5g59210.1 68418.m07420 myosin heavy chain-related contains weak
similarity to Myosin heavy chain, gizzard smooth muscle
(Swiss-Prot:P10587) [Gallus gallus]
Length = 434
Score = 31.1 bits (67), Expect = 0.42
Identities = 23/73 (31%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
Frame = +1
Query: 109 EKLY--NSILTGDYDSAVRQSLEYENQGKGSIIQNVVNNLIIDGSRNTMEYATSCG-SAT 279
EKL+ NS L+ Y ++ S ++ENQ K + QNV ++D R + S G S
Sbjct: 299 EKLFEENSNLSASYQESINISNQWENQVKECLKQNVELREVLDKLRTEQAGSFSRGPSEF 358
Query: 280 DSTLSESTSPITL 318
++ S T ++L
Sbjct: 359 EANGSHGTDTLSL 371
>At4g11270.1 68417.m01823 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
related to TGF-beta resistance-associated protein TRAG
(GI:15624071) {Mus musculus}; similar to beta-transducin
repeats containing protein - Homo sapiens,PID:e1284220;
3' EST no_NP:TC8031
Length = 1446
Score = 29.9 bits (64), Expect = 0.96
Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = -2
Query: 282 VRCRPTACSILHGVPTSVNDQVVNYILDDGALALVLVFQALTDSAVVVTGEDAVVQFL-L 106
+ C ++ VP+S+ + +V +L A+A VL F ++ +S + T D+ V + L
Sbjct: 1142 IECVSSSVGAYQVVPSSIKETLVEVLLPSLAMADVLGFLSIIESQIWSTASDSPVHVVSL 1201
Query: 105 EFFVRTAHA 79
+R A
Sbjct: 1202 RTLIRIIRA 1210
>At2g15490.1 68415.m01772 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 484
Score = 29.9 bits (64), Expect = 0.96
Identities = 19/71 (26%), Positives = 35/71 (49%)
Frame = +1
Query: 121 NSILTGDYDSAVRQSLEYENQGKGSIIQNVVNNLIIDGSRNTMEYATSCGSATDSTLSES 300
N + TG+ + + + E N+GKG II+ ++I + + T CG +STL
Sbjct: 326 NQVGTGENEDWLPKGFEERNKGKGLIIRGWAPQVLILDHKAIGGFVTHCG--WNSTLEGI 383
Query: 301 TSPITLDSSWP 333
+ + + +WP
Sbjct: 384 AAGLPM-VTWP 393
>At4g13310.2 68417.m02080 cytochrome P450 71A20, putative (CYP71A20)
Identical to Cytochrome P450 (SP:Q9T0K2) [Arabidopsis
thaliana]; similar to cytochrome P450 71A4, Solanum
melongena, PIR2:S36805
Length = 390
Score = 29.5 bits (63), Expect = 1.3
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 2/71 (2%)
Frame = +1
Query: 112 KLYNSILTGDYDSAVRQSLEYENQGKGSIIQNVVNNLIIDGSRNTM--EYATSCGSATDS 285
K+ + IL+G D A EY Q K IQN++NN ++ E +
Sbjct: 103 KVVDKILSGGRDVAFAPYGEYWRQMKSICIQNLLNNKMVRSYEKIREEEIKRMIEKLEKA 162
Query: 286 TLSESTSPITL 318
+ S S SP+ L
Sbjct: 163 SCSSSPSPVNL 173
>At4g13310.1 68417.m02081 cytochrome P450 71A20, putative (CYP71A20)
Identical to Cytochrome P450 (SP:Q9T0K2) [Arabidopsis
thaliana]; similar to cytochrome P450 71A4, Solanum
melongena, PIR2:S36805
Length = 497
Score = 29.5 bits (63), Expect = 1.3
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 2/71 (2%)
Frame = +1
Query: 112 KLYNSILTGDYDSAVRQSLEYENQGKGSIIQNVVNNLIIDGSRNTM--EYATSCGSATDS 285
K+ + IL+G D A EY Q K IQN++NN ++ E +
Sbjct: 103 KVVDKILSGGRDVAFAPYGEYWRQMKSICIQNLLNNKMVRSYEKIREEEIKRMIEKLEKA 162
Query: 286 TLSESTSPITL 318
+ S S SP+ L
Sbjct: 163 SCSSSPSPVNL 173
>At4g22200.1 68417.m03209 potassium channel protein 2 (AKT2) (AKT3)
identical to potassium channel [Arabidopsis thaliana]
gi|1100898|gb|AAA97865; Note: also identical to AKT3
[Arabidopsis thaliana] gi|1172218|gb|AAA96153, which is
a truncated version of AKT2, PMID:10852932; member of
the 1 pore, 6 transmembrane (1P/6TM- Shaker-type) K+
channel family, PMID:11500563; identical to cDNA
inward-rectifying K+ channel (AKT3) GI:1172219
Length = 802
Score = 27.5 bits (58), Expect = 5.1
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +1
Query: 178 NQGKGSIIQNVVNNLIIDGSRNTMEYATSCGSATD 282
N G + + + NL+++G+R TME+ S +A++
Sbjct: 301 NLGLTAYLIGNMTNLVVEGTRRTMEFRNSIEAASN 335
>At5g16270.1 68418.m01900 Rad21/Rec8-like family protein weak
similarity to cohesion family protein SYN2 [Arabidopsis
thaliana] GI:12006360; contains Pfam profiles PF04824:
Conserved region of Rad21 / Rec8 like protein, PF04825:
N terminus of Rad21 / Rec8 like protein; supporting cDNA
gi|18157648|gb|AF400129.1|AF400129
Length = 1031
Score = 27.1 bits (57), Expect = 6.8
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = -2
Query: 255 ILHGVPTSVNDQVVNYILDDGALALVLVFQALTDSAVVVTGEDAVVQF 112
+L GV + + VNY+ DD + AL+ V QA +AV + E++ +
Sbjct: 59 LLLGV-VRIYSRKVNYLFDDCSEALLKVKQAFRSAAVDLPPEESTAPY 105
>At1g04120.1 68414.m00401 ABC transporter family protein Strong
similarity to MRP-like ABC transporter gb|U92650 from A.
thaliana and canalicular multi-drug resistance protein
gb|L49379 from Rattus norvegicus
Length = 1514
Score = 27.1 bits (57), Expect = 6.8
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = -2
Query: 267 TACSILHGVPTSVNDQVVNYILDDGALA 184
T C+I H +PT ++ +V +L DG +A
Sbjct: 1455 TVCTIAHRIPTVIDSDLV-LVLSDGRVA 1481
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.130 0.371
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,739,921
Number of Sequences: 28952
Number of extensions: 181730
Number of successful extensions: 501
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 501
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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