BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20260 (600 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_24515| Best HMM Match : No HMM Matches (HMM E-Value=.) 37 0.014 SB_16725| Best HMM Match : DAGAT (HMM E-Value=1e-39) 36 0.033 SB_47020| Best HMM Match : Profilin (HMM E-Value=1.8) 30 1.6 SB_5805| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.2 SB_4880| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.8 SB_47897| Best HMM Match : DUF156 (HMM E-Value=2.9) 27 8.8 >SB_24515| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 117 Score = 36.7 bits (81), Expect = 0.014 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +1 Query: 154 VTKAAIAGHDGNVWAKSEGFEISKDEVAKIVAGLR 258 V +AAI G DG+ WA S GF +S+ E +++ L+ Sbjct: 7 VQRAAIHGLDGSCWATSSGFSVSQQEAMELLKSLK 41 Score = 33.5 bits (73), Expect = 0.13 Identities = 26/69 (37%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Frame = +3 Query: 288 TIAGTRYIYLSGTDH--IIRAKLGKVGVHCM-KTQQAVVISLYEEPIQPQ-QAASVVEKL 455 TI G +Y+ L I KL G C+ T+QA+VI YEE +VVE+L Sbjct: 49 TIGGAKYMMLRNDQESKICYLKLKDKGGFCVCLTKQALVIGGYEESAGGAGNCNNVVEQL 108 Query: 456 GEYLITCGY 482 +YL GY Sbjct: 109 AQYLKESGY 117 >SB_16725| Best HMM Match : DAGAT (HMM E-Value=1e-39) Length = 571 Score = 35.5 bits (78), Expect = 0.033 Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = +3 Query: 282 GVTIAGTRYIYLS-GTDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLG 458 GV + T+Y + H + + G G + T Q ++I Y E + P ++V EKL Sbjct: 504 GVCLNRTKYFVIRVDPGHSLYCRKGNEGAVAVLTSQCLLIGAYSEGMTPGCCSAVTEKLA 563 Query: 459 EYLITCGY 482 +Y G+ Sbjct: 564 DYFRVNGF 571 >SB_47020| Best HMM Match : Profilin (HMM E-Value=1.8) Length = 404 Score = 29.9 bits (64), Expect = 1.6 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +3 Query: 336 IRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLG 458 + K K G+ +KT ++++LY + + P EKLG Sbjct: 36 VYGKHDKTGIVAIKTATLILVALYSQEMSPSICVEASEKLG 76 >SB_5805| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 343 Score = 29.5 bits (63), Expect = 2.2 Identities = 16/39 (41%), Positives = 21/39 (53%) Frame = -2 Query: 392 HSLLCLHAMHADLAKLRADDMVCATEVDVPRARYRHAAT 276 HSL C +A LA +R ++CAT P A RH+ T Sbjct: 71 HSLECTIRCNAPLAVMR-HSLLCATRCHAPLAVMRHSRT 108 Score = 28.3 bits (60), Expect = 5.0 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = -2 Query: 392 HSLLCLHAMHADLAKLRADDMVCATEVDVPRARYRHA 282 HSLLC +A LA +R ++CA P A RH+ Sbjct: 37 HSLLCATRCYAPLAVMR-HSLLCAIRCYAPLAVMRHS 72 >SB_4880| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 961 Score = 28.7 bits (61), Expect = 3.8 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = +1 Query: 127 DKQLMASRCVTKAAIAGHDG-NVWAKSEGFEISKDEVAKIVAGLRM 261 D+ R +TK A+A DG +W + +EI D VA VA LR+ Sbjct: 774 DRASGLGRVLTKEALASKDGETIWFR-HTYEICLDPVACPVANLRL 818 >SB_47897| Best HMM Match : DUF156 (HMM E-Value=2.9) Length = 430 Score = 27.5 bits (58), Expect = 8.8 Identities = 11/27 (40%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = +2 Query: 287 DDSGHAVHLPQWHRPYHPREAWQ-GRR 364 DD+ A P W +P H + AW+ G+R Sbjct: 256 DDTLRARRPPAWRQPVHAKSAWRTGKR 282 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,510,315 Number of Sequences: 59808 Number of extensions: 381972 Number of successful extensions: 979 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 924 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 977 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1451595000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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