BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20260 (600 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 62 3e-10 At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 58 3e-09 At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 58 3e-09 At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 56 2e-08 At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 55 3e-08 At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 33 0.19 At1g74560.1 68414.m08638 nucleosome assembly protein (NAP) famil... 27 9.5 >At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis thaliana] Length = 134 Score = 62.1 bits (144), Expect = 3e-10 Identities = 31/71 (43%), Positives = 44/71 (61%), Gaps = 1/71 (1%) Frame = +3 Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446 L G+ +AG +Y+ + G + +IR K G G+ KT Q++V LYEEP+ P Q VV Sbjct: 63 LAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNMVV 122 Query: 447 EKLGEYLITCG 479 E+LG+YLI G Sbjct: 123 ERLGDYLIEQG 133 Score = 44.4 bits (100), Expect = 6e-05 Identities = 24/54 (44%), Positives = 31/54 (57%), Gaps = 7/54 (12%) Frame = +1 Query: 106 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIV 246 MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIM 54 >At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to SP|Q9FE63 Profilin 5 {Arabidopsis thaliana} Length = 168 Score = 58.4 bits (135), Expect = 3e-09 Identities = 30/71 (42%), Positives = 41/71 (57%), Gaps = 1/71 (1%) Frame = +3 Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446 L G+ + G +Y+ + G + +IR K G GV KT A+V +Y+EP+ P Q VV Sbjct: 97 LAPTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVV 156 Query: 447 EKLGEYLITCG 479 E LGEYLI G Sbjct: 157 ENLGEYLIESG 167 Score = 46.0 bits (104), Expect = 2e-05 Identities = 24/50 (48%), Positives = 31/50 (62%), Gaps = 4/50 (8%) Frame = +1 Query: 106 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKI 243 MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGI 87 >At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis thaliana] Length = 134 Score = 58.4 bits (135), Expect = 3e-09 Identities = 29/71 (40%), Positives = 42/71 (59%), Gaps = 1/71 (1%) Frame = +3 Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446 L G+ +AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q VV Sbjct: 63 LAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNMVV 122 Query: 447 EKLGEYLITCG 479 E+LG+YL+ G Sbjct: 123 ERLGDYLLEQG 133 Score = 44.4 bits (100), Expect = 6e-05 Identities = 22/42 (52%), Positives = 26/42 (61%), Gaps = 6/42 (14%) Frame = +1 Query: 106 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF 213 MSWQ YVD+ LM +T AAI GHDG+VWA+S F Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANF 42 >At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis thaliana]; identical to cDNA profilin (PRF2) GI:9965570 Length = 131 Score = 55.6 bits (128), Expect = 2e-08 Identities = 30/76 (39%), Positives = 42/76 (55%), Gaps = 1/76 (1%) Frame = +3 Query: 255 ENESLLTSGGVTIAGTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQ 431 E L G+ + G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q Sbjct: 55 EEAGHLAPTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQ 114 Query: 432 AASVVEKLGEYLITCG 479 VVE+LG+YLI G Sbjct: 115 CNLVVERLGDYLIESG 130 Score = 44.4 bits (100), Expect = 6e-05 Identities = 24/50 (48%), Positives = 30/50 (60%), Gaps = 4/50 (8%) Frame = +1 Query: 106 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKI 243 MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGI 50 >At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen Ara t 8 identical to profilin 1 (Allergen Ara t 8) SP:Q42449 GI:1353770 from [Arabidopsis thaliana] Length = 131 Score = 55.2 bits (127), Expect = 3e-08 Identities = 29/73 (39%), Positives = 40/73 (54%), Gaps = 1/73 (1%) Frame = +3 Query: 255 ENESLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQ 431 E L G+ + G +Y+ + G +IR K G GV KT QA+V Y+EP+ Q Sbjct: 55 EEPGFLAPTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQ 114 Query: 432 AASVVEKLGEYLI 470 VVE+LG+YLI Sbjct: 115 CNLVVERLGDYLI 127 Score = 41.9 bits (94), Expect = 3e-04 Identities = 21/39 (53%), Positives = 24/39 (61%), Gaps = 3/39 (7%) Frame = +1 Query: 106 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF 213 MSWQ YVD LM +T AAI G DG+VWA+S F Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKF 39 >At1g62420.1 68414.m07042 expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 465 Score = 32.7 bits (71), Expect = 0.19 Identities = 16/63 (25%), Positives = 32/63 (50%) Frame = +2 Query: 263 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 442 I +E RR +H+P W R + + W G ++ T++ ++ + T+ T+A + C Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255 Query: 443 RGE 451 + E Sbjct: 256 KEE 258 >At1g74560.1 68414.m08638 nucleosome assembly protein (NAP) family protein similar to SP|Q01105 SET protein (HLA-DR associated protein II) (PHAPII) (Phosphatase 2A inhibitor I2PP2A) {Homo sapiens}; contains Pfam profile: PF00956 nucleosome assembly protein (NAP) Length = 256 Score = 27.1 bits (57), Expect = 9.5 Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 1/43 (2%) Frame = +2 Query: 413 THPTSAGRICRGEVRRIFNYLWLLEVEARRDLEY-YKIKFFST 538 +HP + G + E ++IF YL LEVE +D++ Y I F T Sbjct: 87 SHP-ALGDLLTEEDQKIFKYLNSLEVEDAKDVKSGYSITFHFT 128 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,698,705 Number of Sequences: 28952 Number of extensions: 258012 Number of successful extensions: 566 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 540 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 561 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1187288784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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