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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20260
         (600 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p...    62   3e-10
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S...    58   3e-09
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p...    58   3e-09
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic...    56   2e-08
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller...    55   3e-08
At1g62420.1 68414.m07042 expressed protein contains Pfam profile...    33   0.19 
At1g74560.1 68414.m08638 nucleosome assembly protein (NAP) famil...    27   9.5  

>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
           profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
           thaliana]
          Length = 134

 Score = 62.1 bits (144), Expect = 3e-10
 Identities = 31/71 (43%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
 Frame = +3

Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446
           L   G+ +AG +Y+ + G  + +IR K G  G+   KT Q++V  LYEEP+ P Q   VV
Sbjct: 63  LAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNMVV 122

Query: 447 EKLGEYLITCG 479
           E+LG+YLI  G
Sbjct: 123 ERLGDYLIEQG 133



 Score = 44.4 bits (100), Expect = 6e-05
 Identities = 24/54 (44%), Positives = 31/54 (57%), Gaps = 7/54 (12%)
 Frame = +1

Query: 106 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIV 246
           MSWQ YVD+ LM          +T AAI GHDG+VWA+S  F +    E+  I+
Sbjct: 1   MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIM 54


>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
           SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
          Length = 168

 Score = 58.4 bits (135), Expect = 3e-09
 Identities = 30/71 (42%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
 Frame = +3

Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446
           L   G+ + G +Y+ + G  + +IR K G  GV   KT  A+V  +Y+EP+ P Q   VV
Sbjct: 97  LAPTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVV 156

Query: 447 EKLGEYLITCG 479
           E LGEYLI  G
Sbjct: 157 ENLGEYLIESG 167



 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 24/50 (48%), Positives = 31/50 (62%), Gaps = 4/50 (8%)
 Frame = +1

Query: 106 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKI 243
           MSWQ YVD  LM   A   +T AAI G DG+VWA+S  F ++  +E+  I
Sbjct: 38  MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGI 87


>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
           profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
           thaliana]
          Length = 134

 Score = 58.4 bits (135), Expect = 3e-09
 Identities = 29/71 (40%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
 Frame = +3

Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446
           L   G+ +AG +Y+ + G    +IR K G  G+   KT Q+ V  +YEEP+ P Q   VV
Sbjct: 63  LAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNMVV 122

Query: 447 EKLGEYLITCG 479
           E+LG+YL+  G
Sbjct: 123 ERLGDYLLEQG 133



 Score = 44.4 bits (100), Expect = 6e-05
 Identities = 22/42 (52%), Positives = 26/42 (61%), Gaps = 6/42 (14%)
 Frame = +1

Query: 106 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF 213
           MSWQ YVD+ LM          +T AAI GHDG+VWA+S  F
Sbjct: 1   MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANF 42


>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
           to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
           thaliana]; identical to cDNA profilin (PRF2) GI:9965570
          Length = 131

 Score = 55.6 bits (128), Expect = 2e-08
 Identities = 30/76 (39%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
 Frame = +3

Query: 255 ENESLLTSGGVTIAGTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQ 431
           E    L   G+ + G +Y+ + G    +IR K G  GV   KT QA+V  +Y+EP+   Q
Sbjct: 55  EEAGHLAPTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQ 114

Query: 432 AASVVEKLGEYLITCG 479
              VVE+LG+YLI  G
Sbjct: 115 CNLVVERLGDYLIESG 130



 Score = 44.4 bits (100), Expect = 6e-05
 Identities = 24/50 (48%), Positives = 30/50 (60%), Gaps = 4/50 (8%)
 Frame = +1

Query: 106 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKI 243
           MSWQ YVD  LM       +T AAI G DG+VWA+S  F ++   E+A I
Sbjct: 1   MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGI 50


>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
           Ara t 8 identical to profilin 1 (Allergen Ara t 8)
           SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
          Length = 131

 Score = 55.2 bits (127), Expect = 3e-08
 Identities = 29/73 (39%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
 Frame = +3

Query: 255 ENESLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQ 431
           E    L   G+ + G +Y+ + G    +IR K G  GV   KT QA+V   Y+EP+   Q
Sbjct: 55  EEPGFLAPTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQ 114

Query: 432 AASVVEKLGEYLI 470
              VVE+LG+YLI
Sbjct: 115 CNLVVERLGDYLI 127



 Score = 41.9 bits (94), Expect = 3e-04
 Identities = 21/39 (53%), Positives = 24/39 (61%), Gaps = 3/39 (7%)
 Frame = +1

Query: 106 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF 213
           MSWQ YVD  LM       +T AAI G DG+VWA+S  F
Sbjct: 1   MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKF 39


>At1g62420.1 68414.m07042 expressed protein contains Pfam profile
           PF04720: Protein of unknown function (DUF506)
          Length = 465

 Score = 32.7 bits (71), Expect = 0.19
 Identities = 16/63 (25%), Positives = 32/63 (50%)
 Frame = +2

Query: 263 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 442
           I  +E RR      +H+P W R  + +  W G    ++ T++  ++ +  T+ T+A + C
Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255

Query: 443 RGE 451
           + E
Sbjct: 256 KEE 258


>At1g74560.1 68414.m08638 nucleosome assembly protein (NAP) family
           protein similar to SP|Q01105 SET protein (HLA-DR
           associated protein II) (PHAPII) (Phosphatase 2A
           inhibitor I2PP2A) {Homo sapiens}; contains Pfam profile:
           PF00956 nucleosome assembly protein (NAP)
          Length = 256

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = +2

Query: 413 THPTSAGRICRGEVRRIFNYLWLLEVEARRDLEY-YKIKFFST 538
           +HP + G +   E ++IF YL  LEVE  +D++  Y I F  T
Sbjct: 87  SHP-ALGDLLTEEDQKIFKYLNSLEVEDAKDVKSGYSITFHFT 128


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,698,705
Number of Sequences: 28952
Number of extensions: 258012
Number of successful extensions: 566
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 561
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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