BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20260
(600 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 62 3e-10
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 58 3e-09
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 58 3e-09
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 56 2e-08
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 55 3e-08
At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 33 0.19
At1g74560.1 68414.m08638 nucleosome assembly protein (NAP) famil... 27 9.5
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 62.1 bits (144), Expect = 3e-10
Identities = 31/71 (43%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
Frame = +3
Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446
L G+ +AG +Y+ + G + +IR K G G+ KT Q++V LYEEP+ P Q VV
Sbjct: 63 LAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNMVV 122
Query: 447 EKLGEYLITCG 479
E+LG+YLI G
Sbjct: 123 ERLGDYLIEQG 133
Score = 44.4 bits (100), Expect = 6e-05
Identities = 24/54 (44%), Positives = 31/54 (57%), Gaps = 7/54 (12%)
Frame = +1
Query: 106 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIV 246
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+
Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIM 54
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 58.4 bits (135), Expect = 3e-09
Identities = 30/71 (42%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
Frame = +3
Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446
L G+ + G +Y+ + G + +IR K G GV KT A+V +Y+EP+ P Q VV
Sbjct: 97 LAPTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVV 156
Query: 447 EKLGEYLITCG 479
E LGEYLI G
Sbjct: 157 ENLGEYLIESG 167
Score = 46.0 bits (104), Expect = 2e-05
Identities = 24/50 (48%), Positives = 31/50 (62%), Gaps = 4/50 (8%)
Frame = +1
Query: 106 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKI 243
MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I
Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGI 87
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 58.4 bits (135), Expect = 3e-09
Identities = 29/71 (40%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
Frame = +3
Query: 270 LTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 446
L G+ +AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q VV
Sbjct: 63 LAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNMVV 122
Query: 447 EKLGEYLITCG 479
E+LG+YL+ G
Sbjct: 123 ERLGDYLLEQG 133
Score = 44.4 bits (100), Expect = 6e-05
Identities = 22/42 (52%), Positives = 26/42 (61%), Gaps = 6/42 (14%)
Frame = +1
Query: 106 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF 213
MSWQ YVD+ LM +T AAI GHDG+VWA+S F
Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANF 42
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 55.6 bits (128), Expect = 2e-08
Identities = 30/76 (39%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
Frame = +3
Query: 255 ENESLLTSGGVTIAGTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQ 431
E L G+ + G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q
Sbjct: 55 EEAGHLAPTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQ 114
Query: 432 AASVVEKLGEYLITCG 479
VVE+LG+YLI G
Sbjct: 115 CNLVVERLGDYLIESG 130
Score = 44.4 bits (100), Expect = 6e-05
Identities = 24/50 (48%), Positives = 30/50 (60%), Gaps = 4/50 (8%)
Frame = +1
Query: 106 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKI 243
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I
Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGI 50
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 55.2 bits (127), Expect = 3e-08
Identities = 29/73 (39%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
Frame = +3
Query: 255 ENESLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQ 431
E L G+ + G +Y+ + G +IR K G GV KT QA+V Y+EP+ Q
Sbjct: 55 EEPGFLAPTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQ 114
Query: 432 AASVVEKLGEYLI 470
VVE+LG+YLI
Sbjct: 115 CNLVVERLGDYLI 127
Score = 41.9 bits (94), Expect = 3e-04
Identities = 21/39 (53%), Positives = 24/39 (61%), Gaps = 3/39 (7%)
Frame = +1
Query: 106 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF 213
MSWQ YVD LM +T AAI G DG+VWA+S F
Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKF 39
>At1g62420.1 68414.m07042 expressed protein contains Pfam profile
PF04720: Protein of unknown function (DUF506)
Length = 465
Score = 32.7 bits (71), Expect = 0.19
Identities = 16/63 (25%), Positives = 32/63 (50%)
Frame = +2
Query: 263 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 442
I +E RR +H+P W R + + W G ++ T++ ++ + T+ T+A + C
Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255
Query: 443 RGE 451
+ E
Sbjct: 256 KEE 258
>At1g74560.1 68414.m08638 nucleosome assembly protein (NAP) family
protein similar to SP|Q01105 SET protein (HLA-DR
associated protein II) (PHAPII) (Phosphatase 2A
inhibitor I2PP2A) {Homo sapiens}; contains Pfam profile:
PF00956 nucleosome assembly protein (NAP)
Length = 256
Score = 27.1 bits (57), Expect = 9.5
Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = +2
Query: 413 THPTSAGRICRGEVRRIFNYLWLLEVEARRDLEY-YKIKFFST 538
+HP + G + E ++IF YL LEVE +D++ Y I F T
Sbjct: 87 SHP-ALGDLLTEEDQKIFKYLNSLEVEDAKDVKSGYSITFHFT 128
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,698,705
Number of Sequences: 28952
Number of extensions: 258012
Number of successful extensions: 566
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 561
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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