BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20257
(419 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55320.1 68416.m06144 ABC transporter family protein similar ... 29 0.97
At2g30320.1 68415.m03690 tRNA pseudouridine synthase family prot... 29 1.7
At1g70740.1 68414.m08154 protein kinase family protein contains ... 28 2.2
At1g01240.3 68414.m00041 expressed protein 28 3.0
At1g01240.2 68414.m00040 expressed protein 28 3.0
At1g01240.1 68414.m00039 expressed protein 28 3.0
At5g42820.2 68418.m05216 U2 snRNP auxiliary factor small subunit... 27 3.9
At5g42820.1 68418.m05215 U2 snRNP auxiliary factor small subunit... 27 3.9
At1g74160.1 68414.m08589 expressed protein 27 6.8
At1g36990.1 68414.m04611 expressed protein contains PS00070: Ald... 27 6.8
At1g04120.1 68414.m00401 ABC transporter family protein Strong s... 26 9.0
>At3g55320.1 68416.m06144 ABC transporter family protein similar to
multidrug resistant P-glycoprotein pmdr1 GI:4204793 from
[Solanum tuberosum]
Length = 1408
Score = 29.5 bits (63), Expect = 0.97
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +1
Query: 298 GSDPRSPRSVVRAPSDARERQHXGTFQESVSTP 396
GS+P SP S + ER H TF +S+P
Sbjct: 769 GSEPESPVSPLLTSDPKNERSHSQTFSRPLSSP 801
>At2g30320.1 68415.m03690 tRNA pseudouridine synthase family protein
similar to pseudouridine synthase [Schizosaccharomyces
pombe] GI:6469502; contains Pfam profile PF01416: tRNA
pseudouridine synthase
Length = 510
Score = 28.7 bits (61), Expect = 1.7
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Frame = +1
Query: 256 TSRSKRRRLSGRTV----GSDPRSPRSVVRAPSDARERQH 363
T RS+ RR S + + G PR P+S+ + S+ RE H
Sbjct: 234 TQRSRYRRKSEQKIKQRNGRPPREPKSIKASESEFREENH 273
>At1g70740.1 68414.m08154 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 425
Score = 28.3 bits (60), Expect = 2.2
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 226 VRWTPARKDLTSRSKRRRLSGRTVGSDPRSPR 321
VR TPA ++RS R +G++ SDP R
Sbjct: 390 VRGTPASSKASTRSNATRSAGQSSSSDPHGKR 421
>At1g01240.3 68414.m00041 expressed protein
Length = 331
Score = 27.9 bits (59), Expect = 3.0
Identities = 20/61 (32%), Positives = 33/61 (54%)
Frame = +2
Query: 221 DPCAGRRREKTLLVVQNVVDFPDGQLVAILGALEALYVPLQMRERDSTEELFKNRSPRRR 400
+ CA + R T+L+ Q Q + +L +EALY LQM++ + EE K + ++R
Sbjct: 233 EACAEKDRVITILLKQASQMLAYKQWLKLL-EMEALY--LQMKKEEEQEEQVKGMNLKKR 289
Query: 401 K 403
K
Sbjct: 290 K 290
>At1g01240.2 68414.m00040 expressed protein
Length = 331
Score = 27.9 bits (59), Expect = 3.0
Identities = 20/61 (32%), Positives = 33/61 (54%)
Frame = +2
Query: 221 DPCAGRRREKTLLVVQNVVDFPDGQLVAILGALEALYVPLQMRERDSTEELFKNRSPRRR 400
+ CA + R T+L+ Q Q + +L +EALY LQM++ + EE K + ++R
Sbjct: 233 EACAEKDRVITILLKQASQMLAYKQWLKLL-EMEALY--LQMKKEEEQEEQVKGMNLKKR 289
Query: 401 K 403
K
Sbjct: 290 K 290
>At1g01240.1 68414.m00039 expressed protein
Length = 331
Score = 27.9 bits (59), Expect = 3.0
Identities = 20/61 (32%), Positives = 33/61 (54%)
Frame = +2
Query: 221 DPCAGRRREKTLLVVQNVVDFPDGQLVAILGALEALYVPLQMRERDSTEELFKNRSPRRR 400
+ CA + R T+L+ Q Q + +L +EALY LQM++ + EE K + ++R
Sbjct: 233 EACAEKDRVITILLKQASQMLAYKQWLKLL-EMEALY--LQMKKEEEQEEQVKGMNLKKR 289
Query: 401 K 403
K
Sbjct: 290 K 290
>At5g42820.2 68418.m05216 U2 snRNP auxiliary factor small subunit,
putative strong similarity to U2 snRNP auxiliary factor,
small subunit [Oryza sativa] GI:3850816
Length = 283
Score = 27.5 bits (58), Expect = 3.9
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = +1
Query: 259 SRSKRRRLSGRTVGSDPRSPRSVVRAPSDARERQH 363
SR RR+L GR S R RS R+ S R+R+H
Sbjct: 177 SRELRRKLFGRYRRSYRRGSRSRSRSISPRRKREH 211
>At5g42820.1 68418.m05215 U2 snRNP auxiliary factor small subunit,
putative strong similarity to U2 snRNP auxiliary factor,
small subunit [Oryza sativa] GI:3850816
Length = 283
Score = 27.5 bits (58), Expect = 3.9
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = +1
Query: 259 SRSKRRRLSGRTVGSDPRSPRSVVRAPSDARERQH 363
SR RR+L GR S R RS R+ S R+R+H
Sbjct: 177 SRELRRKLFGRYRRSYRRGSRSRSRSISPRRKREH 211
>At1g74160.1 68414.m08589 expressed protein
Length = 1030
Score = 26.6 bits (56), Expect = 6.8
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 250 DLTSRSKRRRLSGRTVGSDPRSPRSVVRAPSDARER 357
D SRS R + R + P SPRS+ + P+ R R
Sbjct: 318 DPFSRSLREKNLNRAIRFSPSSPRSLGKDPASPRWR 353
>At1g36990.1 68414.m04611 expressed protein contains PS00070:
Aldehyde dehydrogenases cysteine active site; similar to
high molecular mass nuclear antigen (GI:2754696) [Gallus
gallus];similar to streptococcal hemagglutinin
(GI:8885520) [Streptococcus gordonii] similar to
proteophosphoglycan (GI:5420389) [Leishmania major]
Length = 581
Score = 26.6 bits (56), Expect = 6.8
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = +1
Query: 205 QRTKE*PVRWTPARKDLTSRSKRRRLSGRTVGSDPRSPRSVVRA 336
Q T+ PVR T + K+L S +SG+T+ P + ++ R+
Sbjct: 406 QSTQSAPVRSTNSPKELKGASAFSMISGQTIEKKPSAAQAQSRS 449
>At1g04120.1 68414.m00401 ABC transporter family protein Strong
similarity to MRP-like ABC transporter gb|U92650 from A.
thaliana and canalicular multi-drug resistance protein
gb|L49379 from Rattus norvegicus
Length = 1514
Score = 26.2 bits (55), Expect = 9.0
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 165 KITPYHDARVVKEAAYKGMTRALDAGAKRP 254
K+TPY A +V + L AG+KRP
Sbjct: 226 KVTPYSTAGLVSLITLSWLDPLLSAGSKRP 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,239,435
Number of Sequences: 28952
Number of extensions: 186147
Number of successful extensions: 575
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 564
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 573
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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