BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20256 (489 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharom... 36 0.004 SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 29 0.28 SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit L3|S... 29 0.50 SPBC115.02c |||AFG1 family mitochondrial ATPase|Schizosaccharomy... 27 1.1 SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S... 27 1.1 SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 26 2.7 SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pomb... 25 6.1 SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces... 25 8.1 >SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 886 Score = 35.5 bits (78), Expect = 0.004 Identities = 21/66 (31%), Positives = 34/66 (51%) Frame = +2 Query: 233 QLIITTQVRILNVSLNKLYNLPRGFGSFPVLEILDLTYNNLNEKVLPGNFFIMDRLRALY 412 +++ T++R LN+ N L P LEILD++ N + K LP +F + L+ L Sbjct: 70 EILKFTRLRYLNIRSNVLREFPESLCRLESLEILDISRNKI--KQLPESFGALMNLKVLS 127 Query: 413 LGDNDL 430 + N L Sbjct: 128 ISKNRL 133 Score = 34.7 bits (76), Expect = 0.008 Identities = 20/59 (33%), Positives = 32/59 (54%) Frame = +2 Query: 254 VRILNVSLNKLYNLPRGFGSFPVLEILDLTYNNLNEKVLPGNFFIMDRLRALYLGDNDL 430 + IL++S NK+ LP FG+ L++L ++ N L E LP M L L + +N + Sbjct: 100 LEILDISRNKIKQLPESFGALMNLKVLSISKNRLFE--LPTYIAHMPNLEILKIENNHI 156 >SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1692 Score = 29.5 bits (63), Expect = 0.28 Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +2 Query: 170 SQLEQLRNSKLGQ*QHSRASSQLIITTQVRILNVSLNKLYN-LPRGFGSFPVLEILDLTY 346 ++L QL S+ SS++ +++ L + N L N LP+ G LE +DL+Y Sbjct: 545 TELSQLETLNFSHNLLSQISSKIGSLVKLKHLYLQFNDLSNRLPQEIGLLKNLETIDLSY 604 Query: 347 N 349 N Sbjct: 605 N 605 Score = 29.1 bits (62), Expect = 0.38 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = +2 Query: 263 LNVSLNKLYNLPRGFGSFPVLEILDLTYNN 352 LN++ NKL+ LP L LDL+YNN Sbjct: 507 LNIANNKLFFLPHSTRYLVNLTYLDLSYNN 536 >SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit L3|Schizosaccharomyces pombe|chr 2|||Manual Length = 326 Score = 28.7 bits (61), Expect = 0.50 Identities = 17/47 (36%), Positives = 25/47 (53%) Frame = +2 Query: 236 LIITTQVRILNVSLNKLYNLPRGFGSFPVLEILDLTYNNLNEKVLPG 376 LI T V+ S KL+++ + FG F VLE + Y L E+ + G Sbjct: 69 LIDKTVVQNPQYSNEKLWSIGKVFGDFYVLEYIKCKYPRLPEEAVHG 115 >SPBC115.02c |||AFG1 family mitochondrial ATPase|Schizosaccharomyces pombe|chr 2|||Manual Length = 454 Score = 27.5 bits (58), Expect = 1.1 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -3 Query: 148 ILLWLRDXRVMFSXENKPGISSXLDIPLSTKSI 50 IL W+ + MFS + P ++S L +P K I Sbjct: 89 ILSWISPLKKMFSRKKSPTLTSSLPVPGMPKGI 121 >SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 690 Score = 27.5 bits (58), Expect = 1.1 Identities = 12/14 (85%), Positives = 12/14 (85%) Frame = +3 Query: 438 LPPEIGNLKNLQIL 479 LPPEIG LKNL IL Sbjct: 196 LPPEIGKLKNLVIL 209 >SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 26.2 bits (55), Expect = 2.7 Identities = 13/50 (26%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Frame = -1 Query: 285 SLFNETLRILTWVVMIS*LEALECCYWPSLEFLSCSSWLVQ-QALQRFCC 139 S FN + L W++ S +++ CC + + S++ + AL CC Sbjct: 188 SFFNYIFQNLGWLIRFSFRKSIICCLFTPFSYAILRSYIWRFAALLTSCC 237 >SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1066 Score = 25.0 bits (52), Expect = 6.1 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = -1 Query: 417 PKYKARSLSIIKKFPGNTFSFKLLYVRSNISSTGKE 310 PKY ++++++ PG FS L +R + S+ KE Sbjct: 426 PKYAGHVVAVLQRAPGQVFSGTLGILRPS-SAANKE 460 >SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 298 Score = 24.6 bits (51), Expect = 8.1 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -1 Query: 330 ISSTGKEPKPLGKLYSLFNETL 265 I STG++PK LGK Y E + Sbjct: 208 IFSTGRQPKILGKPYDEMMEAI 229 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,718,934 Number of Sequences: 5004 Number of extensions: 30982 Number of successful extensions: 77 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 71 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 76 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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