BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20249 (399 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 33 0.012 SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.1 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 1.1 SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces pomb... 27 1.4 SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 1.9 SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 25 3.3 SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 4.3 SPCC1795.09 |yps1||aspartic protease Yps1|Schizosaccharomyces po... 24 7.6 SPAC17C9.14 |||Pex19 protein family|Schizosaccharomyces pombe|ch... 24 7.6 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 24 7.6 SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 24 10.0 >SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 33.5 bits (73), Expect = 0.012 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 2/54 (3%) Frame = +2 Query: 62 MLAASAGVV--ELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQGKGSII 217 ++ A+AG+ LS + N+D+ K+ L G Y +A ++ QGKGS+I Sbjct: 91 VMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLI 144 >SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 300 Score = 27.1 bits (57), Expect = 1.1 Identities = 13/26 (50%), Positives = 18/26 (69%) Frame = -3 Query: 187 FQALTDSTVVVAGEDAVVQFLLEVLV 110 F +T V+V EDAVV+F+L +LV Sbjct: 181 FLGVTVQYVMVLPEDAVVEFVLTILV 206 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 27.1 bits (57), Expect = 1.1 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +2 Query: 110 NQDLEEKLYNSILTGDYDSAVRQSLE 187 + DLE+ + ++LTGD SAV+ LE Sbjct: 551 DSDLEKNITEALLTGDVLSAVKACLE 576 >SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 26.6 bits (56), Expect = 1.4 Identities = 15/54 (27%), Positives = 26/54 (48%), Gaps = 2/54 (3%) Frame = +1 Query: 175 SELGI*EPRQGLHHPECS*QPDH*QETNTMEYCYKLWVG--NGQEIVRKYFPLN 330 +ELG+ E L P+ H +E+NT + Y + +G + K+ PL+ Sbjct: 258 TELGLIENPMNLETPKIDYATPHIKESNTPLFTYNFNISGFDGDSCIEKFTPLD 311 >SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 26.2 bits (55), Expect = 1.9 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +3 Query: 75 ARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRAW 185 AR SLNY ++ + SRRN ++P S + W Sbjct: 622 ARESLNYLKSFNKQLSRRNAPDINNPIADFQNSFQNW 658 >SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 25.4 bits (53), Expect = 3.3 Identities = 17/49 (34%), Positives = 22/49 (44%) Frame = +3 Query: 36 NFSLYLRCACSPPARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRA 182 N S + C SP A A + P+TL T ASS T+ + V A Sbjct: 242 NDSFMVGCIYSP-AEAKEHVPKTLRNSNKDLEMTDASSSETSMSIPVHA 289 >SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||Manual Length = 535 Score = 25.0 bits (52), Expect = 4.3 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = -1 Query: 318 VLSNNFLSVADPQLVAVLHGVRLLSMIRLL 229 VLS+ FL+ P L AVL +R +S LL Sbjct: 282 VLSSVFLAWHSPPLQAVLFCIRRISFFNLL 311 >SPCC1795.09 |yps1||aspartic protease Yps1|Schizosaccharomyces pombe|chr 3|||Manual Length = 521 Score = 24.2 bits (50), Expect = 7.6 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = -1 Query: 141 LLYSFSSRSWLEVSADSSTTPALAASMHIANTTRSFILLGAFQI 10 ++YS++S S + SA S +L + +AN SF L A I Sbjct: 465 VVYSYTSSSSMPSSAIPSFNISLISQNAVANAGNSFSPLSAMVI 508 >SPAC17C9.14 |||Pex19 protein family|Schizosaccharomyces pombe|chr 1|||Manual Length = 232 Score = 24.2 bits (50), Expect = 7.6 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = -3 Query: 286 PTACSSTPWCSSLVNDQVVNYILDD 212 P+ + SS NDQ++N IL+D Sbjct: 90 PSGLDGSQLSSSFQNDQLLNVILED 114 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 24.2 bits (50), Expect = 7.6 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = -1 Query: 126 SSRSWLEVSADSSTTPALAASMHI 55 +S S+ V +DSSTTP +++S+ + Sbjct: 318 TSDSFDSVLSDSSTTPTISSSVQV 341 >SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein Mug36|Schizosaccharomyces pombe|chr 2|||Manual Length = 1646 Score = 23.8 bits (49), Expect = 10.0 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = +2 Query: 83 VVELSADTSNQDLEEKLYNSILTGDYDSAVRQSLE 187 VVE T++QD E+++ N L + D + L+ Sbjct: 141 VVEYMNKTASQDFEDEVINEGLESEIDGSSHNLLD 175 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,499,747 Number of Sequences: 5004 Number of extensions: 27558 Number of successful extensions: 95 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 92 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 95 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 134126124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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