BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20249
(399 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 33 0.012
SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.1
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 1.1
SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces pomb... 27 1.4
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 1.9
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 25 3.3
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 4.3
SPCC1795.09 |yps1||aspartic protease Yps1|Schizosaccharomyces po... 24 7.6
SPAC17C9.14 |||Pex19 protein family|Schizosaccharomyces pombe|ch... 24 7.6
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 24 7.6
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 24 10.0
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 33.5 bits (73), Expect = 0.012
Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +2
Query: 62 MLAASAGVV--ELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQGKGSII 217
++ A+AG+ LS + N+D+ K+ L G Y +A ++ QGKGS+I
Sbjct: 91 VMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLI 144
>SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 300
Score = 27.1 bits (57), Expect = 1.1
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -3
Query: 187 FQALTDSTVVVAGEDAVVQFLLEVLV 110
F +T V+V EDAVV+F+L +LV
Sbjct: 181 FLGVTVQYVMVLPEDAVVEFVLTILV 206
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.1 bits (57), Expect = 1.1
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +2
Query: 110 NQDLEEKLYNSILTGDYDSAVRQSLE 187
+ DLE+ + ++LTGD SAV+ LE
Sbjct: 551 DSDLEKNITEALLTGDVLSAVKACLE 576
>SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 339
Score = 26.6 bits (56), Expect = 1.4
Identities = 15/54 (27%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +1
Query: 175 SELGI*EPRQGLHHPECS*QPDH*QETNTMEYCYKLWVG--NGQEIVRKYFPLN 330
+ELG+ E L P+ H +E+NT + Y + +G + K+ PL+
Sbjct: 258 TELGLIENPMNLETPKIDYATPHIKESNTPLFTYNFNISGFDGDSCIEKFTPLD 311
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 1.9
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 75 ARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRAW 185
AR SLNY ++ + SRRN ++P S + W
Sbjct: 622 ARESLNYLKSFNKQLSRRNAPDINNPIADFQNSFQNW 658
>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 25.4 bits (53), Expect = 3.3
Identities = 17/49 (34%), Positives = 22/49 (44%)
Frame = +3
Query: 36 NFSLYLRCACSPPARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRA 182
N S + C SP A A + P+TL T ASS T+ + V A
Sbjct: 242 NDSFMVGCIYSP-AEAKEHVPKTLRNSNKDLEMTDASSSETSMSIPVHA 289
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 25.0 bits (52), Expect = 4.3
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = -1
Query: 318 VLSNNFLSVADPQLVAVLHGVRLLSMIRLL 229
VLS+ FL+ P L AVL +R +S LL
Sbjct: 282 VLSSVFLAWHSPPLQAVLFCIRRISFFNLL 311
>SPCC1795.09 |yps1||aspartic protease Yps1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 521
Score = 24.2 bits (50), Expect = 7.6
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = -1
Query: 141 LLYSFSSRSWLEVSADSSTTPALAASMHIANTTRSFILLGAFQI 10
++YS++S S + SA S +L + +AN SF L A I
Sbjct: 465 VVYSYTSSSSMPSSAIPSFNISLISQNAVANAGNSFSPLSAMVI 508
>SPAC17C9.14 |||Pex19 protein family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 232
Score = 24.2 bits (50), Expect = 7.6
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 286 PTACSSTPWCSSLVNDQVVNYILDD 212
P+ + SS NDQ++N IL+D
Sbjct: 90 PSGLDGSQLSSSFQNDQLLNVILED 114
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 24.2 bits (50), Expect = 7.6
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = -1
Query: 126 SSRSWLEVSADSSTTPALAASMHI 55
+S S+ V +DSSTTP +++S+ +
Sbjct: 318 TSDSFDSVLSDSSTTPTISSSVQV 341
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 23.8 bits (49), Expect = 10.0
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +2
Query: 83 VVELSADTSNQDLEEKLYNSILTGDYDSAVRQSLE 187
VVE T++QD E+++ N L + D + L+
Sbjct: 141 VVEYMNKTASQDFEDEVINEGLESEIDGSSHNLLD 175
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,499,747
Number of Sequences: 5004
Number of extensions: 27558
Number of successful extensions: 95
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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