BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20245
(320 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g16190.1 68417.m02457 cysteine proteinase, putative contains ... 29 0.92
At5g15170.1 68418.m01777 tyrosyl-DNA phosphodiesterase-related s... 28 1.2
At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)... 28 1.6
At5g58850.1 68418.m07374 myb family transcription factor (MYB119... 27 2.1
At4g11320.1 68417.m01828 cysteine proteinase, putative contains ... 27 2.8
At4g11310.1 68417.m01827 cysteine proteinase, putative contains ... 27 3.7
At5g49555.1 68418.m06133 amine oxidase-related contains Pfam pro... 26 6.5
At5g20770.1 68418.m02468 hypothetical protein 25 8.6
At1g26880.1 68414.m03278 60S ribosomal protein L34 (RPL34A) iden... 25 8.6
>At4g16190.1 68417.m02457 cysteine proteinase, putative contains
similarity to papain-like cysteine proteinase isoform I
GI:7381219 from [Ipomoea batatas]
Length = 373
Score = 28.7 bits (61), Expect = 0.92
Identities = 12/47 (25%), Positives = 25/47 (53%), Gaps = 3/47 (6%)
Frame = +2
Query: 188 NPMKEFVRPVHDSA---CPRRVRAFQVKHQRQYASDLEHEKRLNIFR 319
NP+++ V +D F+ K+++ YA+ +EH+ R +F+
Sbjct: 34 NPIRQVVPEENDEQLLNAEHHFTLFKSKYEKTYATQVEHDHRFRVFK 80
>At5g15170.1 68418.m01777 tyrosyl-DNA phosphodiesterase-related
similar to Chain A, Crystal Structure Of Human
Tyrosyl-Dna Phosphodiesterase (Tdp1) [Homo sapiens]
GI:20150581
Length = 605
Score = 28.3 bits (60), Expect = 1.2
Identities = 21/54 (38%), Positives = 30/54 (55%)
Frame = -2
Query: 289 QVARVLPLVFDLKRSNSSWTCAVVDGPHELLHRVEGGEVSRAGPREPSALHVTV 128
QVA ++PL DLK NSS + +GP+ ++ R G VS R S H+T+
Sbjct: 5 QVAYLIPLKADLKEDNSSPRITLSEGPN-IIGR---GNVSIVDKR-LSRKHITI 53
>At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)
family protein similar to cytosolic leucyl-tRNA
synthetase [Candida albicans] GI:9858190; contains Pfam
profile PF00133: tRNA synthetases class I (I, L, M and
V)
Length = 1084
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +2
Query: 107 DPDVFKVDSNMQCTGFPGPGSRHFATF 187
D DVF+ +S C P PG + F+TF
Sbjct: 27 DEDVFRAES---CENLPKPGEKFFSTF 50
>At5g58850.1 68418.m07374 myb family transcription factor (MYB119)
contains Pfam profile: PF00249 myb-like DNA binding
domain
Length = 430
Score = 27.5 bits (58), Expect = 2.1
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 3/53 (5%)
Frame = -1
Query: 191 G*RWRSVASRAPGTQCTAC---YCQL*RRQDRSHQYCNLHNPDSNDRSGSPGA 42
G +W +A PG + + RRQ+ ++ N D+NDR SP A
Sbjct: 176 GNKWAEIAKLIPGRTENSIKNHWNATKRRQNSKRKHKRESNADNNDRDASPSA 228
>At4g11320.1 68417.m01828 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 371
Score = 27.1 bits (57), Expect = 2.8
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +2
Query: 173 HFATFNPMKEFVRPVHDSACPRRVRAFQVKHQRQYASDLEHEKRLNIF 316
H T P + + + D+ ++ VKH + Y S E E+RL IF
Sbjct: 35 HHVTAGPGRR--QGIFDAEATLMFESWMVKHGKVYDSVAEKERRLTIF 80
>At4g11310.1 68417.m01827 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 364
Score = 26.6 bits (56), Expect = 3.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 215 VHDSACPRRVRAFQVKHQRQYASDLEHEKRLNIF 316
V D+ ++ VKH + Y S E E+RL IF
Sbjct: 40 VFDAEASLIFESWMVKHGKVYGSVAEKERRLTIF 73
>At5g49555.1 68418.m06133 amine oxidase-related contains Pfam
profile PF01593: amine oxidase, flavin-containing
Length = 556
Score = 25.8 bits (54), Expect = 6.5
Identities = 15/33 (45%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
Frame = +2
Query: 140 QC--TGFPGPGSRHFATFNPMKEFVRPVHDSAC 232
QC T GPG HF T + E + VH SAC
Sbjct: 364 QCCNTNHSGPGPEHFGTIHIGAESMDEVH-SAC 395
>At5g20770.1 68418.m02468 hypothetical protein
Length = 231
Score = 25.4 bits (53), Expect = 8.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -2
Query: 277 VLPLVFDLKRSNSSWTCAVVDGPHELL 197
V P+ +L + + CA V GP+E+L
Sbjct: 188 VYPICKELPSPDCAMDCATVSGPYEIL 214
>At1g26880.1 68414.m03278 60S ribosomal protein L34 (RPL34A)
identical to GB:Q42351, location of EST 105E2T7,
gb|T22624
Length = 120
Score = 25.4 bits (53), Expect = 8.6
Identities = 15/45 (33%), Positives = 19/45 (42%)
Frame = +1
Query: 151 VPGARLATLRHLQPDEGVRAARPRQRMXXXXXXXXXXXPEAVRER 285
V G R+ + HL+P E R+ R R AVRER
Sbjct: 46 VTGKRIQGIPHLRPSEYKRSRLSRNRRTVNRAYGGVLSGSAVRER 90
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,326,657
Number of Sequences: 28952
Number of extensions: 105070
Number of successful extensions: 279
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 274
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 279
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 350523880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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