BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20244 (357 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po... 57 9e-10 SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharo... 45 3e-06 SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pomb... 30 0.12 SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 3.5 SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 25 4.6 SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 24 6.1 SPBC17D1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 24 8.0 SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1... 24 8.0 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 24 8.0 >SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 351 Score = 56.8 bits (131), Expect = 9e-10 Identities = 32/82 (39%), Positives = 44/82 (53%) Frame = +1 Query: 4 IHEPTSGNTGIGVALATAVCGXGCTIVTPDKNSDEKMSTISLLGAEVVXTPAMAPWESSE 183 I E T+GNTGIG+A G C I P+ S K+ T+ LGAEV P +AP+ + Sbjct: 77 IVEGTAGNTGIGLAHIARARGYKCVIYMPNTQSQAKIDTLKFLGAEVHPVP-VAPFSNPL 135 Query: 184 HFLSVAKRRLLEDPNAISCDQY 249 ++ A+R PNA DQ+ Sbjct: 136 NYNHQARRHAESTPNASWTDQF 157 >SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharomyces pombe|chr 1|||Manual Length = 395 Score = 45.2 bits (102), Expect = 3e-06 Identities = 26/69 (37%), Positives = 36/69 (52%), Gaps = 1/69 (1%) Frame = +1 Query: 4 IHEPTSGNTGIGVALATAVCGXGCTIVTPDKNSDEKMSTISLLGAEVV-XTPAMAPWESS 180 ++E T+G+TGI +A+ G I P S EK + LLGA V TP AP Sbjct: 108 VYEGTAGSTGISIAMLCCSLGYDSRIYMPSDQSKEKSDILELLGAHVQRVTP--APIVDP 165 Query: 181 EHFLSVAKR 207 HF++ A+R Sbjct: 166 NHFVNTARR 174 Score = 25.4 bits (53), Expect = 2.6 Identities = 13/37 (35%), Positives = 18/37 (48%), Gaps = 1/37 (2%) Frame = +3 Query: 249 QEDVNPRTHYEYTAEEILALXPD-VDMIVMGSGTGGT 356 + N + H+ T EI +D + GSGTGGT Sbjct: 196 ENPANWQAHFNSTGPEIWRQCAGKLDAFIAGSGTGGT 232 >SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pombe|chr 2|||Manual Length = 600 Score = 29.9 bits (64), Expect = 0.12 Identities = 15/44 (34%), Positives = 21/44 (47%) Frame = +1 Query: 16 TSGNTGIGVALATAVCGXGCTIVTPDKNSDEKMSTISLLGAEVV 147 ++GN GVA + G TIV P + K + LGA V+ Sbjct: 168 SAGNHAQGVAYSARTLGVKATIVMPQNTPEIKWRNVKRLGANVL 211 >SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 25.0 bits (52), Expect = 3.5 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = +3 Query: 237 LRSVQEDVNPRTHYEYTAEEILALXPDV 320 LR ++D+ R EY A+ I PDV Sbjct: 276 LREPEDDIENRQFSEYVADRIRKSFPDV 303 >SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 24.6 bits (51), Expect = 4.6 Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 4/41 (9%) Frame = +1 Query: 4 IHEPTSGNTGIG-VALATAVC---GXGCTIVTPDKNSDEKM 114 IH T G G G + A C G GC ++ D N D+K+ Sbjct: 142 IHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYDINPDKKL 182 >SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 962 Score = 24.2 bits (50), Expect = 6.1 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +1 Query: 205 RRLLEDPNAISCDQYKKMLILELITNTLLKKY 300 RRLLE + CD K+LI+ ++NT+ ++ Sbjct: 910 RRLLEH---VCCDLTSKLLIVVSLSNTITPEF 938 >SPBC17D1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 23.8 bits (49), Expect = 8.0 Identities = 12/42 (28%), Positives = 20/42 (47%) Frame = -2 Query: 134 PSKLIVLIFSSEFLSGVTMVHPNPQTAVAKATPIPVFPDVGS 9 PS+ + +S + T+ PNP A ATP+ +V + Sbjct: 257 PSRKEIAFSNSSTPTDQTLRPPNPPAANGNATPVTAVNNVSA 298 >SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1 complex subunit Tpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1039 Score = 23.8 bits (49), Expect = 8.0 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = +1 Query: 187 FLSVAKRRLLEDPNAISCDQ 246 +LS KRR +EDP C Q Sbjct: 590 YLSKQKRRPVEDPEVRHCSQ 609 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 23.8 bits (49), Expect = 8.0 Identities = 9/22 (40%), Positives = 12/22 (54%) Frame = -2 Query: 74 HPNPQTAVAKATPIPVFPDVGS 9 HPNP T VA +P+ + S Sbjct: 953 HPNPYTPVAPQSPVAAASRISS 974 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,323,904 Number of Sequences: 5004 Number of extensions: 21655 Number of successful extensions: 66 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 65 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 66 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 107972554 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -