Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20239
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g12080.2 68414.m01397 expressed protein 31 0.67
At3g26420.1 68416.m03295 glycine-rich RNA-binding protein simila... 30 1.2
At5g16730.1 68418.m01959 expressed protein weak similarity to mi... 29 2.7
At1g40133.1 68414.m04768 hypothetical protein 29 2.7
At1g12080.1 68414.m01396 expressed protein 28 3.6
At3g16000.1 68416.m02024 matrix-localized MAR DNA-binding protei... 28 4.7
At3g05900.1 68416.m00664 neurofilament protein-related similar t... 28 4.7
At5g61040.1 68418.m07660 expressed protein 27 6.2
At5g08780.1 68418.m01041 histone H1/H5 family protein contains P... 27 6.2
At1g75720.1 68414.m08796 hypothetical protein 27 6.2
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 27 8.3
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 27 8.3
>At1g12080.2 68414.m01397 expressed protein
Length = 138
Score = 30.7 bits (66), Expect = 0.67
Identities = 19/64 (29%), Positives = 34/64 (53%)
Frame = +2
Query: 53 VEAMEVETKSTEIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKTPSVEEIQEKLKAA 232
VE E + + TE + +E + EVI PV ++A+ +TP+V E ++K +
Sbjct: 62 VEEAEKKDEETEKKTEEKDE---KTEVITETPVVEEEEKKAEEVTETPAVVEEEKKTEVV 118
Query: 233 EERR 244
EE++
Sbjct: 119 EEKQ 122
>At3g26420.1 68416.m03295 glycine-rich RNA-binding protein similar
to RNA-binding protein (RZ-1) GB:BAA12064 [Nicotiana
sylvestris]; contains Pfam profile: PF00076 RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 245
Score = 29.9 bits (64), Expect = 1.2
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +3
Query: 348 HQGGARRQDGDPRGKTRGLHQRAALPSQGSS*G 446
HQGGA R + RG+ RG + + PS G G
Sbjct: 86 HQGGAGRDNDGDRGRDRGYDRDRSRPSGGRGGG 118
>At5g16730.1 68418.m01959 expressed protein weak similarity to
microtubule binding protein D-CLIP-190 [Drosophila
melanogaster] GI:2773363, SMC2-like condensin
[Arabidopsis thaliana] GI:14279543
Length = 853
Score = 28.7 bits (61), Expect = 2.7
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +2
Query: 155 VPVPRRADSPEKTPSVEEIQEKLKAAEERRRSLE 256
V + +SP+ T + +I+E LK A ER SLE
Sbjct: 85 VAAVKGTESPQTTTRLSQIKEDLKKANERISSLE 118
>At1g40133.1 68414.m04768 hypothetical protein
Length = 663
Score = 28.7 bits (61), Expect = 2.7
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 325 QTNNFIVATKEALDAKMETHEEKREAYINE 414
+ NN A +A AKME E +REA++N+
Sbjct: 474 EANNRDEALSQAAAAKMEKEEVEREAFVNK 503
>At1g12080.1 68414.m01396 expressed protein
Length = 104
Score = 28.3 bits (60), Expect = 3.6
Identities = 18/61 (29%), Positives = 30/61 (49%)
Frame = +2
Query: 62 MEVETKSTEIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKTPSVEEIQEKLKAAEER 241
+E E K E + K EVI PV ++A+ +TP+V E ++K + EE+
Sbjct: 29 VETEKKDEETEKKTEEKDEKT-EVITETPVVEEEEKKAEEVTETPAVVEEEKKTEVVEEK 87
Query: 242 R 244
+
Sbjct: 88 Q 88
>At3g16000.1 68416.m02024 matrix-localized MAR DNA-binding
protein-related similar to matrix-localized MAR DNA
binding protein MFP1 GI:1771158 from [Lycopersicon
esculentum]
Length = 726
Score = 27.9 bits (59), Expect = 4.7
Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 13/65 (20%)
Frame = +1
Query: 352 KEALDAKMETHEEKREAYINEL------RSRLKDHLEGV-------EKTRLTLEQQTAEV 492
KE + ++ ++K E +EL R R+KD LEGV +L+++ E+
Sbjct: 489 KERYERNLDAEKQKNEISASELALEKDLRRRVKDELEGVTHELKESSVKNQSLQKELVEI 548
Query: 493 YKAIE 507
YK +E
Sbjct: 549 YKKVE 553
>At3g05900.1 68416.m00664 neurofilament protein-related similar to
NF-180 (GI:632549) [Petromyzon marinus] similar to
Neurofilament triplet H protein (200 kDa neurofilament
protein) (Neurofilament heavy polypeptide) (NF-H)
(Swiss-Prot:P12036) [Homo sapiens]
Length = 673
Score = 27.9 bits (59), Expect = 4.7
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +2
Query: 86 EIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKTPSVEE-IQEKLKAAEERR 244
E + ++S+ EV EPV V + A+ E+ P+VE ++E+ K EE +
Sbjct: 147 ETKIVDVSESTDEAEVQQVEPVDVQPVKDAEKAEEKPTVESVVEEETKDREETK 200
>At5g61040.1 68418.m07660 expressed protein
Length = 590
Score = 27.5 bits (58), Expect = 6.2
Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +1
Query: 319 SEQTNNFIVATKEALDA-KMETHEEKREAYINELRSRLKDHLEGVEKTR 462
S ++F V +E LD +E EEK ++NE+ +L + VEKT+
Sbjct: 521 SHHKDDFKVQEQELLDLIGIEDEEEKSSDFVNEMEKQLIKQI--VEKTK 567
>At5g08780.1 68418.m01041 histone H1/H5 family protein contains Pfam
domain, PF00538: linker histone H1 and H5 family
Length = 457
Score = 27.5 bits (58), Expect = 6.2
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +2
Query: 53 VEAMEVETKSTEIRCQEMSKGGLAYEV 133
VE ++VE E R + S+GG YEV
Sbjct: 229 VEVVDVENSENEARIEANSRGGELYEV 255
>At1g75720.1 68414.m08796 hypothetical protein
Length = 197
Score = 27.5 bits (58), Expect = 6.2
Identities = 18/69 (26%), Positives = 30/69 (43%)
Frame = +2
Query: 14 DAIQLFRSLCRLKVEAMEVETKSTEIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKT 193
++IQ+ SL LK E + + ++R E + V VPR D P ++
Sbjct: 75 ESIQMRNSLSCLKEELERTKQELQKLRVDPGVNETKLDETVFKTKFEVLVPRVDDEPIRS 134
Query: 194 PSVEEIQEK 220
P + + EK
Sbjct: 135 PRLRSMSEK 143
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 27.1 bits (57), Expect = 8.3
Identities = 15/66 (22%), Positives = 35/66 (53%), Gaps = 3/66 (4%)
Frame = +1
Query: 355 EALDAKMETHEEKREAYINELRSRLKDHLEGVEK---TRLTLEQQTAEVYKAIEVX*PQL 525
E L +++ EE+++ + EL ++ + +LEG+E L+ + AE +++V +
Sbjct: 265 EDLKKQLQAVEERKQIAVTELSAKHQKNLEGLEAQVVDALSERDKAAETISSLQVLLAEK 324
Query: 526 PTSVTE 543
+ + E
Sbjct: 325 ESKIAE 330
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 27.1 bits (57), Expect = 8.3
Identities = 15/66 (22%), Positives = 35/66 (53%), Gaps = 3/66 (4%)
Frame = +1
Query: 355 EALDAKMETHEEKREAYINELRSRLKDHLEGVEK---TRLTLEQQTAEVYKAIEVX*PQL 525
E L +++ EE+++ + EL ++ + +LEG+E L+ + AE +++V +
Sbjct: 265 EDLKKQLQAVEERKQIAVTELSAKHQKNLEGLEAQVVDALSERDKAAETISSLQVLLAEK 324
Query: 526 PTSVTE 543
+ + E
Sbjct: 325 ESKIAE 330
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,186,347
Number of Sequences: 28952
Number of extensions: 151375
Number of successful extensions: 682
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 682
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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