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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20235
         (445 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q6C410 Cluster: Similar to sp|P15565 Saccharomyces cere...    62   4e-09
UniRef50_A6RDC7 Cluster: Putative uncharacterized protein; n=1; ...    60   2e-08
UniRef50_Q1DMI8 Cluster: Putative uncharacterized protein; n=1; ...    58   9e-08
UniRef50_P15565 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    56   3e-07
UniRef50_UPI00015B5CEF Cluster: PREDICTED: similar to ENSANGP000...    54   1e-06
UniRef50_Q0UJY7 Cluster: Putative uncharacterized protein; n=1; ...    54   1e-06
UniRef50_A7RIG7 Cluster: Predicted protein; n=2; Nematostella ve...    53   2e-06
UniRef50_Q4WEX8 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    53   2e-06
UniRef50_A6RZH6 Cluster: Putative uncharacterized protein; n=2; ...    53   2e-06
UniRef50_Q5KQ20 Cluster: TRNA (Guanine-N2-)-methyltransferase, p...    52   4e-06
UniRef50_Q7S9X0 Cluster: Putative uncharacterized protein NCU063...    52   7e-06
UniRef50_Q54PG3 Cluster: Putative uncharacterized protein; n=1; ...    51   1e-05
UniRef50_A1CC48 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    51   1e-05
UniRef50_Q386C5 Cluster: N(2), N(2)-dimethylguanosine tRNA methy...    50   2e-05
UniRef50_Q4P6Q4 Cluster: Putative uncharacterized protein; n=1; ...    50   2e-05
UniRef50_Q9P804 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    50   2e-05
UniRef50_UPI0000D56C3B Cluster: PREDICTED: similar to Probable N...    48   7e-05
UniRef50_Q5C276 Cluster: SJCHGC07232 protein; n=1; Schistosoma j...    48   9e-05
UniRef50_Q4QG60 Cluster: N(2), N(2)-dimethylguanosine tRNA methy...    48   1e-04
UniRef50_UPI000023D9FF Cluster: hypothetical protein FG09705.1; ...    47   2e-04
UniRef50_Q9NXH9-2 Cluster: Isoform 2 of Q9NXH9 ; n=2; Homo/Pan/G...    47   2e-04
UniRef50_A4RKM5 Cluster: Putative uncharacterized protein; n=1; ...    47   2e-04
UniRef50_Q9NXH9 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    47   2e-04
UniRef50_A6QP09 Cluster: LOC539613 protein; n=2; Bos taurus|Rep:...    46   3e-04
UniRef50_Q23270 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    46   3e-04
UniRef50_Q9VK89 Cluster: Probable N(2),N(2)-dimethylguanosine tR...    46   4e-04
UniRef50_UPI0000E48B85 Cluster: PREDICTED: hypothetical protein;...    45   6e-04
UniRef50_UPI00004990C0 Cluster: N2,N2-dimethylguanosine tRNA met...    45   6e-04
UniRef50_A0CLP3 Cluster: Chromosome undetermined scaffold_20, wh...    44   0.001
UniRef50_UPI000155D1C2 Cluster: PREDICTED: similar to TRM1 tRNA ...    43   0.003
UniRef50_Q5A863 Cluster: Likely N2,N2-dimethylguanosine-specific...    43   0.003
UniRef50_Q5CQU6 Cluster: Trm1p. N2,N2-dimethylguanosine tRNA met...    43   0.003
UniRef50_Q22HL7 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    41   0.010
UniRef50_A2FDH4 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    41   0.010
UniRef50_Q8RU67 Cluster: Putative tRNA (Guanine-N2-)-Methyltrans...    40   0.024
UniRef50_Q339G6 Cluster: N-dimethylguanosine tRNA methyltransfer...    40   0.024
UniRef50_Q9SRU7 Cluster: Probable N(2),N(2)-dimethylguanosine tR...    40   0.032
UniRef50_Q24333 Cluster: Elastin like protein; n=1; Drosophila m...    38   0.13 
UniRef50_A7AUB1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    37   0.17 
UniRef50_Q5C0A4 Cluster: SJCHGC09205 protein; n=1; Schistosoma j...    37   0.22 
UniRef50_Q8IEC3 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    36   0.39 
UniRef50_Q7QW00 Cluster: GLP_239_44022_45620; n=1; Giardia lambl...    36   0.39 
UniRef50_Q0W1J5 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    36   0.39 
UniRef50_Q2NI56 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    36   0.39 
UniRef50_Q0P6N7 Cluster: Plasma memebrane H+-ATPase; n=1; Planta...    35   0.91 
UniRef50_Q4N4W5 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    35   0.91 
UniRef50_A5JZI1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    35   0.91 
UniRef50_Q9V1P3 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    34   1.2  
UniRef50_Q4YVY3 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    34   1.6  
UniRef50_Q8SR99 Cluster: N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRAN...    34   1.6  
UniRef50_Q589S4 Cluster: HMG protein TCF/LEF; n=1; Dugesia japon...    33   2.8  
UniRef50_Q4YAZ4 Cluster: Putative uncharacterized protein; n=1; ...    33   2.8  
UniRef50_Q8PU28 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    33   2.8  
UniRef50_A7D179 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;...    33   3.7  
UniRef50_Q4J947 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    32   4.8  
UniRef50_Q01C22 Cluster: TRNA methyltransferase; n=1; Ostreococc...    32   6.4  
UniRef50_Q86SD1 Cluster: Calumenin homologue; n=2; Eukaryota|Rep...    32   6.4  
UniRef50_P57705 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    32   6.4  
UniRef50_Q5CDI1 Cluster: Putative uncharacterized protein; n=1; ...    31   8.5  
UniRef50_A5UM08 Cluster: N2,N2-dimethylguanosine tRNA methyltran...    31   8.5  
UniRef50_A4YHH9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;...    31   8.5  
UniRef50_Q97ZH0 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl...    31   8.5  

>UniRef50_Q6C410 Cluster: Similar to sp|P15565 Saccharomyces
           cerevisiae TRM1 N; n=1; Yarrowia lipolytica|Rep: Similar
           to sp|P15565 Saccharomyces cerevisiae TRM1 N - Yarrowia
           lipolytica (Candida lipolytica)
          Length = 492

 Score = 62.5 bits (145), Expect = 4e-09
 Identities = 37/63 (58%), Positives = 46/63 (73%), Gaps = 1/63 (1%)
 Frame = +2

Query: 260 AEKLARFEKKQKKLETVQDEESRGNPE-PKITILEALSATGLXSIRYAKEIPYATNIIAN 436
           AE+ A+  KK+K+ E  ++EES G P  P+  ILEALSA+GL +IRYAKEIP   NIIAN
Sbjct: 50  AEEKAKKNKKRKRDEK-ENEES-GKPHIPEFNILEALSASGLRAIRYAKEIPGVKNIIAN 107

Query: 437 DLS 445
           D S
Sbjct: 108 DFS 110



 Score = 41.5 bits (93), Expect = 0.008
 Identities = 20/38 (52%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
 Frame = +3

Query: 141 IKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFIE 251
           I EG+A I    E +VFYNPVQ+FNRDLS+  +  + E
Sbjct: 10  ITEGKANILFPKENQVFYNPVQQFNRDLSVTCIRAWSE 47


>UniRef50_A6RDC7 Cluster: Putative uncharacterized protein; n=1;
           Ajellomyces capsulatus NAm1|Rep: Putative
           uncharacterized protein - Ajellomyces capsulatus NAm1
          Length = 797

 Score = 60.5 bits (140), Expect = 2e-08
 Identities = 30/60 (50%), Positives = 39/60 (65%)
 Frame = +2

Query: 266 KLARFEKKQKKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           K  R E++     T     +  N  P  +IL+ALSATGL ++RYAKEIP+AT I+ANDLS
Sbjct: 184 KCVRAEQQDTATGTTTTTVTEQNKIPPFSILDALSATGLRALRYAKEIPFATRIVANDLS 243



 Score = 37.9 bits (84), Expect = 0.097
 Identities = 14/26 (53%), Positives = 20/26 (76%)
 Frame = +3

Query: 174 TEKVFYNPVQEFNRDLSIAVLTLFIE 251
           T+ VFYNP+Q+FNRDLS+  +  + E
Sbjct: 103 TQSVFYNPIQQFNRDLSVLAIRAYGE 128


>UniRef50_Q1DMI8 Cluster: Putative uncharacterized protein; n=1;
           Coccidioides immitis|Rep: Putative uncharacterized
           protein - Coccidioides immitis
          Length = 853

 Score = 58.0 bits (134), Expect = 9e-08
 Identities = 25/35 (71%), Positives = 31/35 (88%)
 Frame = +2

Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           P  TIL+ALSATGL ++RYAKEIP+AT ++ANDLS
Sbjct: 296 PSFTILDALSATGLRALRYAKEIPFATQVVANDLS 330



 Score = 35.1 bits (77), Expect = 0.69
 Identities = 13/23 (56%), Positives = 18/23 (78%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTLFIE 251
           VFYNP+Q+FNRDLS+  +  + E
Sbjct: 194 VFYNPIQQFNRDLSVLAIRAYGE 216


>UniRef50_P15565 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase, mitochondrial precursor (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=9;
           Saccharomycetales|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase, mitochondrial precursor (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Saccharomyces
           cerevisiae (Baker's yeast)
          Length = 570

 Score = 56.0 bits (129), Expect = 3e-07
 Identities = 30/55 (54%), Positives = 38/55 (69%), Gaps = 3/55 (5%)
 Frame = +2

Query: 287 KQKKLETVQDEESRGNP---EPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           K++K+     +E+ GN    EP I ILEALSATGL +IRYA EIP+   +IANDL
Sbjct: 111 KRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVREVIANDL 165



 Score = 44.0 bits (99), Expect = 0.001
 Identities = 19/39 (48%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
 Frame = +3

Query: 123 ISNLKTIKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVL 236
           I +   +KEG+A+I     E VFYNP+Q+FNRDLS+  +
Sbjct: 39  IEDFNIVKEGKAEILFPKKETVFYNPIQQFNRDLSVTCI 77


>UniRef50_UPI00015B5CEF Cluster: PREDICTED: similar to
           ENSANGP00000010185; n=1; Nasonia vitripennis|Rep:
           PREDICTED: similar to ENSANGP00000010185 - Nasonia
           vitripennis
          Length = 529

 Score = 54.0 bits (124), Expect = 1e-06
 Identities = 24/38 (63%), Positives = 32/38 (84%)
 Frame = +3

Query: 141 IKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254
           ++EGQA+I L  + VFYNPVQEFNRDLSIAVL+ + ++
Sbjct: 13  LEEGQAKILLEKKNVFYNPVQEFNRDLSIAVLSQYSKE 50



 Score = 53.6 bits (123), Expect = 2e-06
 Identities = 23/52 (44%), Positives = 37/52 (71%)
 Frame = +2

Query: 290 QKKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           Q  +++ ++++   + +  + +LEALSATGL SIRYAKE+P    I+AND+S
Sbjct: 54  QSSIDSAKEKQKDDSQKTGLRVLEALSATGLRSIRYAKEVPGMAEIVANDIS 105


>UniRef50_Q0UJY7 Cluster: Putative uncharacterized protein; n=1;
           Phaeosphaeria nodorum|Rep: Putative uncharacterized
           protein - Phaeosphaeria nodorum (Septoria nodorum)
          Length = 677

 Score = 54.0 bits (124), Expect = 1e-06
 Identities = 23/35 (65%), Positives = 31/35 (88%)
 Frame = +2

Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           P  +IL+ALSATGL ++RYAKEIP+AT++ AND+S
Sbjct: 204 PPFSILDALSATGLRALRYAKEIPFATSVTANDMS 238



 Score = 40.3 bits (90), Expect = 0.018
 Identities = 15/27 (55%), Positives = 22/27 (81%)
 Frame = +3

Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIEDI 257
           ++VFYNP+Q+FNRDLS+  +  F +DI
Sbjct: 64  QQVFYNPIQQFNRDLSVLAIKTFGQDI 90


>UniRef50_A7RIG7 Cluster: Predicted protein; n=2; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 607

 Score = 53.2 bits (122), Expect = 2e-06
 Identities = 26/40 (65%), Positives = 31/40 (77%), Gaps = 1/40 (2%)
 Frame = +3

Query: 141 IKEGQAQICLTTEK-VFYNPVQEFNRDLSIAVLTLFIEDI 257
           I+EG+A+I    EK VFYNPVQEFNRD+S AV+ L  EDI
Sbjct: 24  IREGKAEIIFPAEKAVFYNPVQEFNRDISSAVIRLVCEDI 63



 Score = 44.8 bits (101), Expect = 8e-04
 Identities = 19/34 (55%), Positives = 28/34 (82%)
 Frame = +2

Query: 344 KITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           ++TILE L+A+GL S+RYA E+P   ++IAND+S
Sbjct: 93  QVTILEGLAASGLRSVRYALEVPGIHHVIANDIS 126


>UniRef50_Q4WEX8 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase; n=10; Trichocomaceae|Rep:
           N2,N2-dimethylguanosine tRNA methyltransferase -
           Aspergillus fumigatus (Sartorya fumigata)
          Length = 747

 Score = 53.2 bits (122), Expect = 2e-06
 Identities = 23/35 (65%), Positives = 29/35 (82%)
 Frame = +2

Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           P  TIL+ALSATGL ++RYA EIP+ T ++ANDLS
Sbjct: 196 PSFTILDALSATGLRALRYASEIPFTTCVVANDLS 230



 Score = 36.7 bits (81), Expect = 0.22
 Identities = 13/27 (48%), Positives = 20/27 (74%)
 Frame = +3

Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIEDI 257
           + VFYNP+Q+FNRDLS+  +  + E +
Sbjct: 112 QSVFYNPIQQFNRDLSVLAIKAYGEHV 138


>UniRef50_A6RZH6 Cluster: Putative uncharacterized protein; n=2;
           Sclerotiniaceae|Rep: Putative uncharacterized protein -
           Botryotinia fuckeliana B05.10
          Length = 688

 Score = 53.2 bits (122), Expect = 2e-06
 Identities = 25/38 (65%), Positives = 32/38 (84%), Gaps = 1/38 (2%)
 Frame = +2

Query: 332 NP-EPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           NP  P+  IL+ALSATGL ++RYA+EIP+AT+I ANDL
Sbjct: 197 NPINPRFQILDALSATGLRALRYAQEIPFATSITANDL 234



 Score = 39.5 bits (88), Expect = 0.032
 Identities = 18/41 (43%), Positives = 27/41 (65%)
 Frame = +3

Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDI 257
           +T  +GQ Q+    + VFYNP+Q+FNRDLS+  +  + E I
Sbjct: 46  QTTPKGQNQV----QSVFYNPIQQFNRDLSVLAIKAYGESI 82


>UniRef50_Q5KQ20 Cluster: TRNA (Guanine-N2-)-methyltransferase,
           putative; n=1; Filobasidiella neoformans|Rep: TRNA
           (Guanine-N2-)-methyltransferase, putative - Cryptococcus
           neoformans (Filobasidiella neoformans)
          Length = 666

 Score = 52.4 bits (120), Expect = 4e-06
 Identities = 25/35 (71%), Positives = 28/35 (80%)
 Frame = +2

Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           P I ILEAL+ATGL SIRYAKEIP    ++ANDLS
Sbjct: 144 PSINILEALAATGLRSIRYAKEIPNVKYVLANDLS 178



 Score = 35.5 bits (78), Expect = 0.52
 Identities = 16/35 (45%), Positives = 21/35 (60%)
 Frame = +3

Query: 147 EGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIE 251
           E    I L  E  F NPVQ +NRD+S+AV+  + E
Sbjct: 24  ESTTTIFLPNEGAFLNPVQHYNRDMSVAVIRAWNE 58


>UniRef50_Q7S9X0 Cluster: Putative uncharacterized protein
           NCU06336.1; n=2; Sordariales|Rep: Putative
           uncharacterized protein NCU06336.1 - Neurospora crassa
          Length = 683

 Score = 51.6 bits (118), Expect = 7e-06
 Identities = 24/52 (46%), Positives = 35/52 (67%)
 Frame = +2

Query: 287 KQKKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           +Q   E    E  +G  +P  T+L+ALSA+GL ++RYA EIP+ T++ ANDL
Sbjct: 157 EQGDKEATNGEAKKGK-QPPFTVLDALSASGLRALRYAHEIPFLTSVTANDL 207



 Score = 32.7 bits (71), Expect = 3.7
 Identities = 10/22 (45%), Positives = 18/22 (81%)
 Frame = +3

Query: 180 KVFYNPVQEFNRDLSIAVLTLF 245
           +VFYNP+Q++NRDL++  +  +
Sbjct: 56  QVFYNPIQQYNRDLTVLAIKAY 77


>UniRef50_Q54PG3 Cluster: Putative uncharacterized protein; n=1;
           Dictyostelium discoideum AX4|Rep: Putative
           uncharacterized protein - Dictyostelium discoideum AX4
          Length = 604

 Score = 50.8 bits (116), Expect = 1e-05
 Identities = 24/44 (54%), Positives = 32/44 (72%), Gaps = 1/44 (2%)
 Frame = +3

Query: 126 SNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFIED 254
           SN+ T+ E  A I    + +VFYNPVQEFNRD+SI ++ LFIE+
Sbjct: 49  SNIVTVIENSATILYNNQNEVFYNPVQEFNRDMSILMIKLFIEE 92



 Score = 46.4 bits (105), Expect = 3e-04
 Identities = 25/40 (62%), Positives = 30/40 (75%), Gaps = 1/40 (2%)
 Frame = +2

Query: 326 RGNPEPKITILEALSATGLXSIRYAKEIPYATN-IIANDL 442
           RG P  KI +LEAL+ATGL SIRYAKEI    + I+AND+
Sbjct: 100 RGKPFKKIRVLEALAATGLRSIRYAKEIGDDLDYILANDI 139


>UniRef50_A1CC48 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase, putative; n=2; Aspergillus|Rep:
           N2,N2-dimethylguanosine tRNA methyltransferase, putative
           - Aspergillus clavatus
          Length = 1045

 Score = 50.8 bits (116), Expect = 1e-05
 Identities = 25/44 (56%), Positives = 33/44 (75%), Gaps = 3/44 (6%)
 Frame = +2

Query: 323 SRGNPEPKI---TILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           S  +PE  +   TIL+ALSATGL ++RYA EIP+ T ++ANDLS
Sbjct: 466 SAPHPEQSVIPFTILDALSATGLRALRYASEIPFVTCVVANDLS 509



 Score = 36.7 bits (81), Expect = 0.22
 Identities = 13/28 (46%), Positives = 21/28 (75%)
 Frame = +3

Query: 174 TEKVFYNPVQEFNRDLSIAVLTLFIEDI 257
           ++ VFYNP+Q+FNRDLS+  +  + E +
Sbjct: 391 SQSVFYNPIQQFNRDLSVLAIKAYGEHL 418


>UniRef50_Q386C5 Cluster: N(2), N(2)-dimethylguanosine tRNA
           methyltransferase, putative; n=3; Trypanosoma|Rep: N(2),
           N(2)-dimethylguanosine tRNA methyltransferase, putative
           - Trypanosoma brucei
          Length = 802

 Score = 50.4 bits (115), Expect = 2e-05
 Identities = 25/44 (56%), Positives = 32/44 (72%)
 Frame = +2

Query: 311 QDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           ++ +++G     ITILEALSATGL +IRY KEIP    IIAND+
Sbjct: 191 EEPKNKGGTRRGITILEALSATGLRAIRYYKEIPDVRFIIANDI 234



 Score = 33.1 bits (72), Expect = 2.8
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = +3

Query: 177 EKVFYNPVQEFNRDLSIAVLTLF 245
           + VFYNP Q  NRDLS+ V+  F
Sbjct: 163 QAVFYNPAQVVNRDLSVCVIACF 185


>UniRef50_Q4P6Q4 Cluster: Putative uncharacterized protein; n=1;
           Ustilago maydis|Rep: Putative uncharacterized protein -
           Ustilago maydis (Smut fungus)
          Length = 711

 Score = 50.0 bits (114), Expect = 2e-05
 Identities = 24/34 (70%), Positives = 28/34 (82%)
 Frame = +2

Query: 344 KITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           + T+LEALSATGL SIRYAKEIP    ++ANDLS
Sbjct: 172 QFTLLEALSATGLRSIRYAKEIPLLRWVLANDLS 205



 Score = 33.9 bits (74), Expect = 1.6
 Identities = 17/37 (45%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = +3

Query: 144 KEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLFIE 251
           +E  A I + + E  F NPVQEFNRDLS   +  + E
Sbjct: 28  RENSATIVMPSAEAAFLNPVQEFNRDLSTLAIRTWSE 64


>UniRef50_Q9P804 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=1;
           Schizosaccharomyces pombe|Rep:
           N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC
           2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           (tRNA 2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) -
           Schizosaccharomyces pombe (Fission yeast)
          Length = 548

 Score = 50.0 bits (114), Expect = 2e-05
 Identities = 24/31 (77%), Positives = 26/31 (83%)
 Frame = +2

Query: 350 TILEALSATGLXSIRYAKEIPYATNIIANDL 442
           TILEALSATGL SIRYAKE+P    I+ANDL
Sbjct: 126 TILEALSATGLRSIRYAKELPNVKRILANDL 156



 Score = 38.3 bits (85), Expect = 0.074
 Identities = 18/39 (46%), Positives = 26/39 (66%), Gaps = 1/39 (2%)
 Frame = +3

Query: 138 TIKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVLTLFIE 251
           ++ EG A I  +   +VFYNPVQ+FNRDLS+  +  + E
Sbjct: 31  SLTEGSAIIPFSNPNEVFYNPVQQFNRDLSVTAIRAWSE 69


>UniRef50_UPI0000D56C3B Cluster: PREDICTED: similar to Probable
           N(2),N(2)-dimethylguanosine tRNA methyltransferase
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=2;
           Endopterygota|Rep: PREDICTED: similar to Probable
           N(2),N(2)-dimethylguanosine tRNA methyltransferase
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Tribolium
           castaneum
          Length = 501

 Score = 48.4 bits (110), Expect = 7e-05
 Identities = 24/33 (72%), Positives = 27/33 (81%)
 Frame = +2

Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           I+ILEALSATGL SIRYAKE+     IIAND+S
Sbjct: 69  ISILEALSATGLRSIRYAKEVKGVKEIIANDIS 101



 Score = 47.6 bits (108), Expect = 1e-04
 Identities = 25/38 (65%), Positives = 27/38 (71%)
 Frame = +3

Query: 141 IKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254
           I EG A+I  T   VFYNPVQEFNRDLSI VL  F +D
Sbjct: 14  ITEGLAKI-KTLGSVFYNPVQEFNRDLSIVVLNTFAKD 50


>UniRef50_Q5C276 Cluster: SJCHGC07232 protein; n=1; Schistosoma
           japonicum|Rep: SJCHGC07232 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 222

 Score = 48.0 bits (109), Expect = 9e-05
 Identities = 22/41 (53%), Positives = 31/41 (75%)
 Frame = +3

Query: 123 ISNLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLF 245
           ++N   +KEG A++ L  + VFYNPVQEFNRDL+IA++  F
Sbjct: 15  MTNPSFVKEGSAEVMLP-DAVFYNPVQEFNRDLTIAMVNQF 54



 Score = 42.7 bits (96), Expect = 0.003
 Identities = 20/31 (64%), Positives = 26/31 (83%)
 Frame = +2

Query: 353 ILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           ILEALSA+G+ SIR A E+P  ++IIAND+S
Sbjct: 82  ILEALSASGIRSIRMALEVPNVSSIIANDIS 112


>UniRef50_Q4QG60 Cluster: N(2), N(2)-dimethylguanosine tRNA
           methyltransferase, putative; n=3; Leishmania|Rep: N(2),
           N(2)-dimethylguanosine tRNA methyltransferase, putative
           - Leishmania major
          Length = 718

 Score = 47.6 bits (108), Expect = 1e-04
 Identities = 25/39 (64%), Positives = 27/39 (69%)
 Frame = +2

Query: 326 RGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           RG     ITILEALSATGL +IRY KEI     IIAND+
Sbjct: 98  RGGTNEGITILEALSATGLRAIRYYKEITNVRYIIANDM 136



 Score = 36.3 bits (80), Expect = 0.30
 Identities = 16/35 (45%), Positives = 23/35 (65%)
 Frame = +3

Query: 141 IKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLF 245
           + + +A   L  + VFYNP Q  NRDLSI+V+ +F
Sbjct: 53  LSDEEADNQLVDQAVFYNPAQVVNRDLSISVIEVF 87


>UniRef50_UPI000023D9FF Cluster: hypothetical protein FG09705.1;
           n=1; Gibberella zeae PH-1|Rep: hypothetical protein
           FG09705.1 - Gibberella zeae PH-1
          Length = 1026

 Score = 47.2 bits (107), Expect = 2e-04
 Identities = 20/36 (55%), Positives = 29/36 (80%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           +P   IL+ALSA+GL ++RYA E+P+ T++ ANDLS
Sbjct: 133 KPAFRILDALSASGLRALRYAHELPFVTSVKANDLS 168



 Score = 35.1 bits (77), Expect = 0.69
 Identities = 12/25 (48%), Positives = 20/25 (80%)
 Frame = +3

Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIE 251
           ++VFYNP+Q++NRDLS+  +  + E
Sbjct: 44  QQVFYNPIQQYNRDLSVLAIKTYGE 68


>UniRef50_Q9NXH9-2 Cluster: Isoform 2 of Q9NXH9 ; n=2;
           Homo/Pan/Gorilla group|Rep: Isoform 2 of Q9NXH9 - Homo
           sapiens (Human)
          Length = 630

 Score = 46.8 bits (106), Expect = 2e-04
 Identities = 22/37 (59%), Positives = 28/37 (75%), Gaps = 1/37 (2%)
 Frame = +3

Query: 138 TIKEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLF 245
           T+ EG A+I   +  +VFYNPVQEFNRDL+ AV+T F
Sbjct: 56  TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF 92



 Score = 39.1 bits (87), Expect = 0.042
 Identities = 17/36 (47%), Positives = 26/36 (72%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           E  + +LE L+A+GL SIR+A E+P   +++AND S
Sbjct: 151 EEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDAS 186


>UniRef50_A4RKM5 Cluster: Putative uncharacterized protein; n=1;
           Magnaporthe grisea|Rep: Putative uncharacterized protein
           - Magnaporthe grisea (Rice blast fungus) (Pyricularia
           grisea)
          Length = 702

 Score = 46.8 bits (106), Expect = 2e-04
 Identities = 19/34 (55%), Positives = 28/34 (82%)
 Frame = +2

Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           P++ IL+ALSATGL ++RYA E+P+ T + +NDL
Sbjct: 196 PRLKILDALSATGLRALRYAHELPFPTMVTSNDL 229



 Score = 35.1 bits (77), Expect = 0.69
 Identities = 12/23 (52%), Positives = 18/23 (78%)
 Frame = +3

Query: 177 EKVFYNPVQEFNRDLSIAVLTLF 245
           + VFYNP+Q+FNRDLS+  +  +
Sbjct: 69  QTVFYNPIQQFNRDLSVLAIKAY 91


>UniRef50_Q9NXH9 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=17;
           Euteleostomi|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Homo sapiens
           (Human)
          Length = 659

 Score = 46.8 bits (106), Expect = 2e-04
 Identities = 22/37 (59%), Positives = 28/37 (75%), Gaps = 1/37 (2%)
 Frame = +3

Query: 138 TIKEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLF 245
           T+ EG A+I   +  +VFYNPVQEFNRDL+ AV+T F
Sbjct: 56  TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF 92



 Score = 39.1 bits (87), Expect = 0.042
 Identities = 17/36 (47%), Positives = 26/36 (72%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           E  + +LE L+A+GL SIR+A E+P   +++AND S
Sbjct: 151 EEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDAS 186


>UniRef50_A6QP09 Cluster: LOC539613 protein; n=2; Bos taurus|Rep:
           LOC539613 protein - Bos taurus (Bovine)
          Length = 691

 Score = 46.4 bits (105), Expect = 3e-04
 Identities = 23/37 (62%), Positives = 27/37 (72%), Gaps = 1/37 (2%)
 Frame = +3

Query: 138 TIKEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLF 245
           TI EG A+I      +VFYNPVQEFNRDL+ AV+T F
Sbjct: 87  TITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEF 123



 Score = 41.1 bits (92), Expect = 0.010
 Identities = 17/36 (47%), Positives = 27/36 (75%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           E  + +LE L+A+GL SIR+A+E+P   +++AND S
Sbjct: 182 EEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDAS 217


>UniRef50_Q23270 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=2;
           Caenorhabditis|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Caenorhabditis
           elegans
          Length = 526

 Score = 46.4 bits (105), Expect = 3e-04
 Identities = 24/62 (38%), Positives = 42/62 (67%), Gaps = 5/62 (8%)
 Frame = +2

Query: 275 RFEKKQKKLETVQDEESRGNP-----EPKITILEALSATGLXSIRYAKEIPYATNIIAND 439
           ++ ++QK+L+T ++   + N      + KI IL+ALSA+GL ++R++KE+P    I+AND
Sbjct: 63  KWAEEQKQLKTEEEPPKKKNKLAINEDGKIRILDALSASGLRALRFSKEVPNVGFIMAND 122

Query: 440 LS 445
            S
Sbjct: 123 FS 124



 Score = 45.2 bits (102), Expect = 6e-04
 Identities = 23/49 (46%), Positives = 31/49 (63%)
 Frame = +3

Query: 108 MRMETISNLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254
           ++ E    +  I+EGQA++      VFYNPVQEFNRDL++ VL  F  D
Sbjct: 13  IKSEDKEEVTVIQEGQAKVGFHGP-VFYNPVQEFNRDLTVTVLRQFSAD 60


>UniRef50_Q9VK89 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=4; Diptera|Rep:
           Probable N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Drosophila
           melanogaster (Fruit fly)
          Length = 578

 Score = 46.0 bits (104), Expect = 4e-04
 Identities = 29/67 (43%), Positives = 41/67 (61%), Gaps = 12/67 (17%)
 Frame = +2

Query: 281 EKKQKKLETVQDEESRGNPEPK------------ITILEALSATGLXSIRYAKEIPYATN 424
           +K++KK++  +DE++   PE              + ILEAL+ATGL SIRYA+EI     
Sbjct: 67  KKQRKKVKEQEDEKTTPVPEDPPVYEAGTRYEDGLRILEALAATGLRSIRYAQEIAGVRQ 126

Query: 425 IIANDLS 445
           I+ANDLS
Sbjct: 127 IVANDLS 133



 Score = 44.4 bits (100), Expect = 0.001
 Identities = 22/39 (56%), Positives = 28/39 (71%)
 Frame = +3

Query: 129 NLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLF 245
           N   I+E  A+I ++   VFYNPVQEFNRDLSIA L ++
Sbjct: 16  NENVIRERNAEI-VSGGNVFYNPVQEFNRDLSIAALNVY 53


>UniRef50_UPI0000E48B85 Cluster: PREDICTED: hypothetical protein;
           n=2; Strongylocentrotus purpuratus|Rep: PREDICTED:
           hypothetical protein - Strongylocentrotus purpuratus
          Length = 556

 Score = 45.2 bits (102), Expect = 6e-04
 Identities = 21/39 (53%), Positives = 28/39 (71%), Gaps = 1/39 (2%)
 Frame = +3

Query: 141 IKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVLTLFIED 254
           ++EG+A+I      +VFYNP Q  NRDLS+AV  LF+ED
Sbjct: 13  VEEGRARIVFPNPNEVFYNPAQVINRDLSLAVTGLFVED 51



 Score = 45.2 bits (102), Expect = 6e-04
 Identities = 21/36 (58%), Positives = 26/36 (72%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           E  IT LEALSA+GL S+R AKE+P    ++AND S
Sbjct: 92  EEGITFLEALSASGLRSVRIAKEVPGIKRVVANDFS 127


>UniRef50_UPI00004990C0 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase; n=1; Entamoeba histolytica
           HM-1:IMSS|Rep: N2,N2-dimethylguanosine tRNA
           methyltransferase - Entamoeba histolytica HM-1:IMSS
          Length = 489

 Score = 45.2 bits (102), Expect = 6e-04
 Identities = 21/45 (46%), Positives = 30/45 (66%), Gaps = 2/45 (4%)
 Frame = +3

Query: 126 SNLKTIKEGQAQICLTTEKV--FYNPVQEFNRDLSIAVLTLFIED 254
           S    IKEGQA +    E+   FYNPVQE NRDLSI++++ ++ +
Sbjct: 7   SKYTPIKEGQATVLFPKEEAGAFYNPVQELNRDLSISIISKYLRN 51



 Score = 42.7 bits (96), Expect = 0.003
 Identities = 23/53 (43%), Positives = 35/53 (66%), Gaps = 2/53 (3%)
 Frame = +2

Query: 293 KKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATN--IIANDLS 445
           K L   ++E  + N +    I+EAL+A+GL S+RYAKE+P   +  +IAND+S
Sbjct: 47  KYLRNKENELKQKNKQYHPLIVEALAASGLRSVRYAKELPQDIDFKVIANDIS 99


>UniRef50_A0CLP3 Cluster: Chromosome undetermined scaffold_20, whole
           genome shotgun sequence; n=1; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_20,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 487

 Score = 44.0 bits (99), Expect = 0.001
 Identities = 23/38 (60%), Positives = 29/38 (76%)
 Frame = +2

Query: 332 NPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           N +P ITIL+ALSA+GL S+R+A+EI     I ANDLS
Sbjct: 67  NRQP-ITILDALSASGLRSVRFAQEIQNVKEIYANDLS 103



 Score = 35.1 bits (77), Expect = 0.69
 Identities = 15/29 (51%), Positives = 20/29 (68%)
 Frame = +3

Query: 168 LTTEKVFYNPVQEFNRDLSIAVLTLFIED 254
           L  E+VFYNP Q  NRDL++ V+  F E+
Sbjct: 39  LIEEEVFYNPAQIINRDLTVLVIDTFQEN 67


>UniRef50_UPI000155D1C2 Cluster: PREDICTED: similar to TRM1 tRNA
           methyltransferase 1 homolog (S. cerevisiae); n=1;
           Ornithorhynchus anatinus|Rep: PREDICTED: similar to TRM1
           tRNA methyltransferase 1 homolog (S. cerevisiae) -
           Ornithorhynchus anatinus
          Length = 223

 Score = 43.2 bits (97), Expect = 0.003
 Identities = 21/36 (58%), Positives = 26/36 (72%), Gaps = 1/36 (2%)
 Frame = +3

Query: 141 IKEGQAQICLT-TEKVFYNPVQEFNRDLSIAVLTLF 245
           + EG A+I      +VFYNPVQEFNRDL+ AV+T F
Sbjct: 59  VTEGAARIVFPGANEVFYNPVQEFNRDLTCAVVTEF 94


>UniRef50_Q5A863 Cluster: Likely N2,N2-dimethylguanosine-specific
           tRNA methyltransferase; n=5; Saccharomycetales|Rep:
           Likely N2,N2-dimethylguanosine-specific tRNA
           methyltransferase - Candida albicans (Yeast)
          Length = 556

 Score = 43.2 bits (97), Expect = 0.003
 Identities = 22/46 (47%), Positives = 31/46 (67%), Gaps = 2/46 (4%)
 Frame = +3

Query: 120 TISN-LKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFIE 251
           TIS    T++EG+A I    + +VFYNP+Q+FNRDLSI  +  + E
Sbjct: 35  TISQEFNTVQEGKATILTPKQDEVFYNPIQQFNRDLSIMAIKAYDE 80



 Score = 40.3 bits (90), Expect = 0.018
 Identities = 20/55 (36%), Positives = 34/55 (61%), Gaps = 1/55 (1%)
 Frame = +2

Query: 281 EKKQKKLETVQDEESRGNPEPK-ITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           E + +K++ ++ +      +   + ILE+L+A+GL S RY  EIP A  I+AND+
Sbjct: 80  EIRHEKIQAIKKKSKNKRTKLNGLKILESLAASGLRSCRYGLEIPEAGKIVANDM 134


>UniRef50_Q5CQU6 Cluster: Trm1p. N2,N2-dimethylguanosine tRNA
           methyltransferase; n=2; Cryptosporidium|Rep: Trm1p.
           N2,N2-dimethylguanosine tRNA methyltransferase -
           Cryptosporidium parvum Iowa II
          Length = 637

 Score = 42.7 bits (96), Expect = 0.003
 Identities = 18/41 (43%), Positives = 28/41 (68%)
 Frame = +3

Query: 126 SNLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFI 248
           S+ + I EG+ +I  T + +FYNP Q FNRD+S+ V+  F+
Sbjct: 15  SSSEYIHEGKVKIHKTNDDIFYNPAQVFNRDISLIVIKSFL 55



 Score = 33.5 bits (73), Expect = 2.1
 Identities = 15/39 (38%), Positives = 25/39 (64%), Gaps = 1/39 (2%)
 Frame = +2

Query: 329 GNPEPKITILEALSATGLXSIRYAKEIPY-ATNIIANDL 442
           G+    +T+LE L A+GL SIRY KE+ +   +++  D+
Sbjct: 104 GSSRFSVTVLEPLGASGLRSIRYIKELSHEIDHVVCGDI 142


>UniRef50_Q22HL7 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase family protein; n=1; Tetrahymena
           thermophila SB210|Rep: N2,N2-dimethylguanosine tRNA
           methyltransferase family protein - Tetrahymena
           thermophila SB210
          Length = 557

 Score = 41.1 bits (92), Expect = 0.010
 Identities = 20/43 (46%), Positives = 30/43 (69%)
 Frame = +2

Query: 317 EESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           +E+R      ITIL+ALSA+GL +IR++KE+     + AND+S
Sbjct: 91  KETRKEKFDGITILDALSASGLRAIRFSKELKDIKKVYANDIS 133



 Score = 37.1 bits (82), Expect = 0.17
 Identities = 15/24 (62%), Positives = 20/24 (83%)
 Frame = +3

Query: 180 KVFYNPVQEFNRDLSIAVLTLFIE 251
           +VFYNPVQ FNRDL++ V+ +F E
Sbjct: 65  EVFYNPVQVFNRDLTVLVIHMFKE 88


>UniRef50_A2FDH4 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase family protein; n=4; Trichomonas
           vaginalis G3|Rep: N2,N2-dimethylguanosine tRNA
           methyltransferase family protein - Trichomonas vaginalis
           G3
          Length = 476

 Score = 41.1 bits (92), Expect = 0.010
 Identities = 21/39 (53%), Positives = 26/39 (66%)
 Frame = +2

Query: 326 RGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           R +    I+I EA +A+GL SIRYAKEI     II+NDL
Sbjct: 55  RNSVRDDISIFEAFAASGLRSIRYAKEITGIKEIISNDL 93



 Score = 35.9 bits (79), Expect = 0.39
 Identities = 14/21 (66%), Positives = 18/21 (85%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTLF 245
           VFYNPVQ+FNRDL+ AV+  +
Sbjct: 34  VFYNPVQQFNRDLTCAVIQAY 54


>UniRef50_Q8RU67 Cluster: Putative tRNA
           (Guanine-N2-)-Methyltransferase; n=3; Oryza sativa|Rep:
           Putative tRNA (Guanine-N2-)-Methyltransferase - Oryza
           sativa (Rice)
          Length = 490

 Score = 39.9 bits (89), Expect = 0.024
 Identities = 22/38 (57%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
 Frame = +3

Query: 138 TIKEGQAQICLT-TEKVFYNPVQEFNRDLSIAVLTLFI 248
           TI+EG+A+I    +  VFYN  Q  NRDLSIAVL  FI
Sbjct: 53  TIREGRAEIFADDSNSVFYNKAQVNNRDLSIAVLRSFI 90



 Score = 31.9 bits (69), Expect = 6.4
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +2

Query: 353 ILEALSATGLXSIRYAKEIPYATNIIAND 439
           +LEAL+A+GL +IRYA E+     +IA D
Sbjct: 141 VLEALAASGLRAIRYALEVDGIGEVIAVD 169


>UniRef50_Q339G6 Cluster: N-dimethylguanosine tRNA
           methyltransferase, putative, expressed; n=2; Oryza
           sativa (japonica cultivar-group)|Rep:
           N-dimethylguanosine tRNA methyltransferase, putative,
           expressed - Oryza sativa subsp. japonica (Rice)
          Length = 555

 Score = 39.9 bits (89), Expect = 0.024
 Identities = 22/38 (57%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
 Frame = +3

Query: 138 TIKEGQAQICLT-TEKVFYNPVQEFNRDLSIAVLTLFI 248
           TI+EG+A+I    +  VFYN  Q  NRDLSIAVL  FI
Sbjct: 53  TIREGRAEIFADDSNSVFYNKAQVNNRDLSIAVLRSFI 90



 Score = 31.9 bits (69), Expect = 6.4
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +2

Query: 353 ILEALSATGLXSIRYAKEIPYATNIIAND 439
           +LEAL+A+GL +IRYA E+     +IA D
Sbjct: 141 VLEALAASGLRAIRYALEVDGIGEVIAVD 169


>UniRef50_Q9SRU7 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=13;
           Viridiplantae|Rep: Probable N(2),N(2)-dimethylguanosine
           tRNA methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Arabidopsis
           thaliana (Mouse-ear cress)
          Length = 599

 Score = 39.5 bits (88), Expect = 0.032
 Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 2/47 (4%)
 Frame = +3

Query: 114 MET-ISNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFI 248
           MET +++   IKEG+A+I +  + +VF+N  Q  NRD+SIAVL  F+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFL 47



 Score = 33.1 bits (72), Expect = 2.8
 Identities = 17/35 (48%), Positives = 25/35 (71%), Gaps = 2/35 (5%)
 Frame = +2

Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIA--NDLS 445
           I I +ALSA+GL ++RYA+EI     ++A  NDL+
Sbjct: 132 ILIFQALSASGLRALRYAREIEGIGQVVALDNDLA 166


>UniRef50_Q24333 Cluster: Elastin like protein; n=1; Drosophila
           melanogaster|Rep: Elastin like protein - Drosophila
           melanogaster (Fruit fly)
          Length = 110

 Score = 37.5 bits (83), Expect = 0.13
 Identities = 25/54 (46%), Positives = 28/54 (51%), Gaps = 7/54 (12%)
 Frame = +2

Query: 29  TAXXXXLELVDPPGCRNSA----RAHF-TDLYENGNY--IKLKNYKRRPSPDMF 169
           TA    LELVDPPGCRNSA    RA+  + LY    Y  + L  Y   P P  F
Sbjct: 4   TAVAAALELVDPPGCRNSARDRQRANLNSQLYAGYPYPGVYLPQYPAYPQPAQF 57


>UniRef50_A7AUB1 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase, putative; n=1; Babesia bovis|Rep:
           N(2),N(2)-dimethylguanosine tRNA methyltransferase,
           putative - Babesia bovis
          Length = 544

 Score = 37.1 bits (82), Expect = 0.17
 Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 3/41 (7%)
 Frame = +3

Query: 138 TIKEGQ---AQICLTTEKVFYNPVQEFNRDLSIAVLTLFIE 251
           T+ EG    + + +  + +FYNP Q FNRDLS+ VL  F+E
Sbjct: 6   TVTEGLVTVSSLSVDGQPLFYNPPQVFNRDLSLIVLKTFVE 46



 Score = 35.9 bits (79), Expect = 0.39
 Identities = 16/33 (48%), Positives = 23/33 (69%), Gaps = 1/33 (3%)
 Frame = +2

Query: 347 ITILEALSATGLXSIRYAKEI-PYATNIIANDL 442
           + +LE L+ATG+ SIRY KE+  Y  ++  NDL
Sbjct: 70  VNVLEMLAATGIRSIRYLKELGDYVNHVYINDL 102


>UniRef50_Q5C0A4 Cluster: SJCHGC09205 protein; n=1; Schistosoma
          japonicum|Rep: SJCHGC09205 protein - Schistosoma
          japonicum (Blood fluke)
          Length = 215

 Score = 36.7 bits (81), Expect = 0.22
 Identities = 16/20 (80%), Positives = 16/20 (80%)
 Frame = +2

Query: 29 TAXXXXLELVDPPGCRNSAR 88
          TA    LELVDPPGCRNSAR
Sbjct: 5  TAVAAALELVDPPGCRNSAR 24


>UniRef50_Q8IEC3 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase, putative; n=2; Plasmodium|Rep:
           N2,N2-dimethylguanosine tRNA methyltransferase, putative
           - Plasmodium falciparum (isolate 3D7)
          Length = 804

 Score = 35.9 bits (79), Expect = 0.39
 Identities = 22/54 (40%), Positives = 31/54 (57%), Gaps = 4/54 (7%)
 Frame = +2

Query: 293 KKLETVQDEESRGNPE---PKITILEALSATGLXSIRYAKEIPYATN-IIANDL 442
           K LE    E+++ N +       I+E LSA+G+ SIRYAKE+    N I  ND+
Sbjct: 85  KALEIFMKEKNKNNEKIIFRGFNIIELLSASGMRSIRYAKELEDTINHITTNDI 138



 Score = 33.1 bits (72), Expect = 2.8
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = +3

Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVL 236
           K I EG  +I      +FYN  Q FNRD+SI ++
Sbjct: 51  KFIYEGSVKIKNKKNHIFYNKAQVFNRDMSIVLI 84


>UniRef50_Q7QW00 Cluster: GLP_239_44022_45620; n=1; Giardia lamblia
           ATCC 50803|Rep: GLP_239_44022_45620 - Giardia lamblia
           ATCC 50803
          Length = 532

 Score = 35.9 bits (79), Expect = 0.39
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = +3

Query: 138 TIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDI 257
           T  EG         +VF+NP Q FNRD+S+ V+  +++ I
Sbjct: 20  THTEGTVSFTYNPSQVFFNPTQIFNRDISLLVVDQYLQHI 59


>UniRef50_Q0W1J5 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase; n=1; uncultured methanogenic archaeon
           RC-I|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase - Uncultured methanogenic archaeon
           RC-I
          Length = 365

 Score = 35.9 bits (79), Expect = 0.39
 Identities = 18/36 (50%), Positives = 24/36 (66%)
 Frame = +3

Query: 132 LKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLT 239
           +  I+EG+  I +  E VFYNP  E NRDL++A LT
Sbjct: 1   MAVIREGKVAIEMG-EGVFYNPRMEMNRDLNVACLT 35


>UniRef50_Q2NI56 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=2;
           Methanobacteriaceae|Rep: N(2),N(2)-dimethylguanosine
           tRNA methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Methanosphaera
           stadtmanae (strain DSM 3091)
          Length = 390

 Score = 35.9 bits (79), Expect = 0.39
 Identities = 13/25 (52%), Positives = 20/25 (80%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTLFIEDI 257
           VFYNPV E NRD+S+AV+  + +++
Sbjct: 27  VFYNPVMEMNRDISVAVINQYRKEV 51


>UniRef50_Q0P6N7 Cluster: Plasma memebrane H+-ATPase; n=1;
          Plantago major|Rep: Plasma memebrane H+-ATPase -
          Plantago major (Common plantain)
          Length = 106

 Score = 34.7 bits (76), Expect = 0.91
 Identities = 14/14 (100%), Positives = 14/14 (100%)
 Frame = +2

Query: 47 LELVDPPGCRNSAR 88
          LELVDPPGCRNSAR
Sbjct: 11 LELVDPPGCRNSAR 24


>UniRef50_Q4N4W5 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase, putative; n=2; Theileria|Rep:
           N2,N2-dimethylguanosine tRNA methyltransferase, putative
           - Theileria parva
          Length = 599

 Score = 34.7 bits (76), Expect = 0.91
 Identities = 15/22 (68%), Positives = 17/22 (77%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTLFI 248
           +FYNP Q FNRDLS+ VL  FI
Sbjct: 43  LFYNPPQVFNRDLSLLVLKTFI 64



 Score = 31.9 bits (69), Expect = 6.4
 Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
 Frame = +2

Query: 347 ITILEALSATGLXSIRYAKEI-PYATNIIANDL 442
           + ILE+L+ATG+  IRY +E+ P    +  NDL
Sbjct: 83  VNILESLAATGIRGIRYLRELGPLVGMVTFNDL 115


>UniRef50_A5JZI1 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase, putative; n=2; Plasmodium|Rep:
           N(2),N(2)-dimethylguanosine tRNA methyltransferase,
           putative - Plasmodium vivax
          Length = 949

 Score = 34.7 bits (76), Expect = 0.91
 Identities = 16/36 (44%), Positives = 22/36 (61%)
 Frame = +3

Query: 129 NLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVL 236
           N K I EG  +I   +  +FYN  Q FNRD+SI ++
Sbjct: 67  NSKYIFEGSVKIKNKSNHIFYNKAQVFNRDMSIILI 102



 Score = 34.7 bits (76), Expect = 0.91
 Identities = 17/31 (54%), Positives = 23/31 (74%), Gaps = 1/31 (3%)
 Frame = +2

Query: 353 ILEALSATGLXSIRYAKEIPYATN-IIANDL 442
           ++E LSA+G+ SIRY KE+    N IIAND+
Sbjct: 126 VVELLSASGIRSIRYVKELRETINHIIANDI 156


>UniRef50_Q9V1P3 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=6;
           Thermococcaceae|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Pyrococcus abyssi
          Length = 383

 Score = 34.3 bits (75), Expect = 1.2
 Identities = 19/36 (52%), Positives = 26/36 (72%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           +P+I +L+ALSATG+  IR+A E P A  I  ND+S
Sbjct: 51  KPRI-VLDALSATGIRGIRFALETP-AEEIWMNDIS 84


>UniRef50_Q4YVY3 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase, putative; n=3; Plasmodium
           (Vinckeia)|Rep: N2,N2-dimethylguanosine tRNA
           methyltransferase, putative - Plasmodium berghei
          Length = 811

 Score = 33.9 bits (74), Expect = 1.6
 Identities = 16/36 (44%), Positives = 22/36 (61%)
 Frame = +3

Query: 129 NLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVL 236
           N K I EG  +I    + +FYN  Q FNRD+SI ++
Sbjct: 52  NNKHIYEGCVKIKNKNKHIFYNKAQVFNRDMSIVLI 87



 Score = 33.5 bits (73), Expect = 2.1
 Identities = 15/31 (48%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
 Frame = +2

Query: 353 ILEALSATGLXSIRYAKEIPYATN-IIANDL 442
           ++E LSA+G+ SIRY KE+    N I+ ND+
Sbjct: 111 VIELLSASGIRSIRYVKELKETINHIVTNDI 141


>UniRef50_Q8SR99 Cluster: N2,N2-DIMETHYLGUANOSINE tRNA
           METHYLTRANSFERASE; n=1; Encephalitozoon cuniculi|Rep:
           N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRANSFERASE -
           Encephalitozoon cuniculi
          Length = 432

 Score = 33.9 bits (74), Expect = 1.6
 Identities = 16/40 (40%), Positives = 25/40 (62%)
 Frame = +3

Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254
           +TI E ++ +    E  F+NP Q+FNRD+SI V+    +D
Sbjct: 9   ETISE-ESTVITKNEWTFFNPAQKFNRDISIEVVKECFKD 47



 Score = 33.5 bits (73), Expect = 2.1
 Identities = 17/33 (51%), Positives = 23/33 (69%)
 Frame = +2

Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIANDLS 445
           I +L+A+SATGL  IRY KEI  +  +  ND+S
Sbjct: 51  IRVLDAMSATGLRGIRYLKEIGNSI-VYLNDIS 82


>UniRef50_Q589S4 Cluster: HMG protein TCF/LEF; n=1; Dugesia
           japonica|Rep: HMG protein TCF/LEF - Dugesia japonica
           (Planarian)
          Length = 263

 Score = 33.1 bits (72), Expect = 2.8
 Identities = 13/17 (76%), Positives = 14/17 (82%)
 Frame = +2

Query: 50  ELVDPPGCRNSARAHFT 100
           +LVDPPGCRNSAR   T
Sbjct: 5   QLVDPPGCRNSARGECT 21


>UniRef50_Q4YAZ4 Cluster: Putative uncharacterized protein; n=1;
          Plasmodium berghei|Rep: Putative uncharacterized
          protein - Plasmodium berghei
          Length = 89

 Score = 33.1 bits (72), Expect = 2.8
 Identities = 14/18 (77%), Positives = 14/18 (77%)
 Frame = +2

Query: 29 TAXXXXLELVDPPGCRNS 82
          TA    LELVDPPGCRNS
Sbjct: 11 TAVAAALELVDPPGCRNS 28


>UniRef50_Q8PU28 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=5;
           Methanosarcinaceae|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Methanosarcina
           mazei (Methanosarcina frisia)
          Length = 388

 Score = 33.1 bits (72), Expect = 2.8
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTLFIE 251
           VFYNP  E NRD+++A    F+E
Sbjct: 31  VFYNPEMELNRDINVAATAAFVE 53


>UniRef50_A7D179 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;
           n=1; Halorubrum lacusprofundi ATCC 49239|Rep: tRNA
           (Guanine-N(2)-)-methyltransferase - Halorubrum
           lacusprofundi ATCC 49239
          Length = 433

 Score = 32.7 bits (71), Expect = 3.7
 Identities = 12/21 (57%), Positives = 17/21 (80%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTLF 245
           VF+NP QE NRD+++AVL  +
Sbjct: 79  VFFNPTQELNRDVTVAVLRAY 99


>UniRef50_Q4J947 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase; n=1; Sulfolobus acidocaldarius|Rep:
           N(2),N(2)-dimethylguanosine tRNA methyltransferase -
           Sulfolobus acidocaldarius
          Length = 382

 Score = 32.3 bits (70), Expect = 4.8
 Identities = 16/35 (45%), Positives = 23/35 (65%)
 Frame = +2

Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442
           +PK ++++ LSATG+  IRY  EI     II ND+
Sbjct: 55  KPK-SLIDGLSATGVRGIRYGLEINGVEEIILNDI 88


>UniRef50_Q01C22 Cluster: TRNA methyltransferase; n=1; Ostreococcus
           tauri|Rep: TRNA methyltransferase - Ostreococcus tauri
          Length = 123

 Score = 31.9 bits (69), Expect = 6.4
 Identities = 17/37 (45%), Positives = 23/37 (62%), Gaps = 2/37 (5%)
 Frame = +3

Query: 141 IKEGQAQIC--LTTEKVFYNPVQEFNRDLSIAVLTLF 245
           I+EG A+      T+ VFYN  Q  NRD+S+AV+  F
Sbjct: 19  IREGAARALQRAETDDVFYNKPQVVNRDMSLAVIREF 55


>UniRef50_Q86SD1 Cluster: Calumenin homologue; n=2; Eukaryota|Rep:
           Calumenin homologue - Ciona intestinalis (Transparent
           sea squirt)
          Length = 308

 Score = 31.9 bits (69), Expect = 6.4
 Identities = 18/45 (40%), Positives = 22/45 (48%)
 Frame = +2

Query: 29  TAXXXXLELVDPPGCRNSARAHFTDLYENGNYIKLKNYKRRPSPD 163
           TA    LELVDPPGCR       T+ Y      + K +KR  S +
Sbjct: 124 TAVAAALELVDPPGCRLVHEVDETEDYRKMYEREEKRWKRADSDE 168


>UniRef50_P57705 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=4;
           Halobacteriaceae|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Halobacterium
           salinarium (Halobacterium halobium)
          Length = 371

 Score = 31.9 bits (69), Expect = 6.4
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +3

Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIE 251
           + VF+N  QE NRDL++A L  F E
Sbjct: 24  DDVFFNATQELNRDLTVATLAAFRE 48


>UniRef50_Q5CDI1 Cluster: Putative uncharacterized protein; n=1;
           Cryptosporidium hominis|Rep: Putative uncharacterized
           protein - Cryptosporidium hominis
          Length = 72

 Score = 31.5 bits (68), Expect = 8.5
 Identities = 15/23 (65%), Positives = 16/23 (69%)
 Frame = -2

Query: 96  KWARAEFLQPGGSTSSRXXXXAV 28
           K  + EFLQPGGSTSSR    AV
Sbjct: 39  KCEKIEFLQPGGSTSSRAAATAV 61


>UniRef50_A5UM08 Cluster: N2,N2-dimethylguanosine tRNA
           methyltransferase, Trm1; n=1; Methanobrevibacter smithii
           ATCC 35061|Rep: N2,N2-dimethylguanosine tRNA
           methyltransferase, Trm1 - Methanobrevibacter smithii
           (strain PS / ATCC 35061 / DSM 861)
          Length = 388

 Score = 31.5 bits (68), Expect = 8.5
 Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 7/45 (15%)
 Frame = +3

Query: 132 LKTIKEGQAQICL-------TTEKVFYNPVQEFNRDLSIAVLTLF 245
           +KT++EG  +I         +   VFYNP  E NRD+SI  L  F
Sbjct: 6   IKTVEEGLTKIQFPEFDKISSDAPVFYNPHMELNRDISILALQTF 50


>UniRef50_A4YHH9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;
           n=1; Metallosphaera sedula DSM 5348|Rep: TRNA
           (Guanine-N(2)-)-methyltransferase - Metallosphaera
           sedula DSM 5348
          Length = 382

 Score = 31.5 bits (68), Expect = 8.5
 Identities = 13/30 (43%), Positives = 20/30 (66%)
 Frame = +2

Query: 350 TILEALSATGLXSIRYAKEIPYATNIIAND 439
           ++L+A+SATG+  IRY KE      ++ ND
Sbjct: 58  SVLDAMSATGVRGIRYVKESGVKGEVLFND 87


>UniRef50_Q97ZH0 Cluster: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase); n=2;
           Sulfolobus|Rep: N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2- dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase) - Sulfolobus
           solfataricus
          Length = 378

 Score = 31.5 bits (68), Expect = 8.5
 Identities = 12/20 (60%), Positives = 16/20 (80%)
 Frame = +3

Query: 183 VFYNPVQEFNRDLSIAVLTL 242
           VFYNP   FNRDLS+ V+++
Sbjct: 34  VFYNPKMTFNRDLSVIVVSI 53


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 316,864,154
Number of Sequences: 1657284
Number of extensions: 4605538
Number of successful extensions: 14531
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 14107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14518
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 22761518346
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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