BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20235 (445 letters) Database: uniref50 1,657,284 sequences; 575,637,011 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UniRef50_Q6C410 Cluster: Similar to sp|P15565 Saccharomyces cere... 62 4e-09 UniRef50_A6RDC7 Cluster: Putative uncharacterized protein; n=1; ... 60 2e-08 UniRef50_Q1DMI8 Cluster: Putative uncharacterized protein; n=1; ... 58 9e-08 UniRef50_P15565 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 56 3e-07 UniRef50_UPI00015B5CEF Cluster: PREDICTED: similar to ENSANGP000... 54 1e-06 UniRef50_Q0UJY7 Cluster: Putative uncharacterized protein; n=1; ... 54 1e-06 UniRef50_A7RIG7 Cluster: Predicted protein; n=2; Nematostella ve... 53 2e-06 UniRef50_Q4WEX8 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 53 2e-06 UniRef50_A6RZH6 Cluster: Putative uncharacterized protein; n=2; ... 53 2e-06 UniRef50_Q5KQ20 Cluster: TRNA (Guanine-N2-)-methyltransferase, p... 52 4e-06 UniRef50_Q7S9X0 Cluster: Putative uncharacterized protein NCU063... 52 7e-06 UniRef50_Q54PG3 Cluster: Putative uncharacterized protein; n=1; ... 51 1e-05 UniRef50_A1CC48 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 51 1e-05 UniRef50_Q386C5 Cluster: N(2), N(2)-dimethylguanosine tRNA methy... 50 2e-05 UniRef50_Q4P6Q4 Cluster: Putative uncharacterized protein; n=1; ... 50 2e-05 UniRef50_Q9P804 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 50 2e-05 UniRef50_UPI0000D56C3B Cluster: PREDICTED: similar to Probable N... 48 7e-05 UniRef50_Q5C276 Cluster: SJCHGC07232 protein; n=1; Schistosoma j... 48 9e-05 UniRef50_Q4QG60 Cluster: N(2), N(2)-dimethylguanosine tRNA methy... 48 1e-04 UniRef50_UPI000023D9FF Cluster: hypothetical protein FG09705.1; ... 47 2e-04 UniRef50_Q9NXH9-2 Cluster: Isoform 2 of Q9NXH9 ; n=2; Homo/Pan/G... 47 2e-04 UniRef50_A4RKM5 Cluster: Putative uncharacterized protein; n=1; ... 47 2e-04 UniRef50_Q9NXH9 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 47 2e-04 UniRef50_A6QP09 Cluster: LOC539613 protein; n=2; Bos taurus|Rep:... 46 3e-04 UniRef50_Q23270 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 46 3e-04 UniRef50_Q9VK89 Cluster: Probable N(2),N(2)-dimethylguanosine tR... 46 4e-04 UniRef50_UPI0000E48B85 Cluster: PREDICTED: hypothetical protein;... 45 6e-04 UniRef50_UPI00004990C0 Cluster: N2,N2-dimethylguanosine tRNA met... 45 6e-04 UniRef50_A0CLP3 Cluster: Chromosome undetermined scaffold_20, wh... 44 0.001 UniRef50_UPI000155D1C2 Cluster: PREDICTED: similar to TRM1 tRNA ... 43 0.003 UniRef50_Q5A863 Cluster: Likely N2,N2-dimethylguanosine-specific... 43 0.003 UniRef50_Q5CQU6 Cluster: Trm1p. N2,N2-dimethylguanosine tRNA met... 43 0.003 UniRef50_Q22HL7 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 41 0.010 UniRef50_A2FDH4 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 41 0.010 UniRef50_Q8RU67 Cluster: Putative tRNA (Guanine-N2-)-Methyltrans... 40 0.024 UniRef50_Q339G6 Cluster: N-dimethylguanosine tRNA methyltransfer... 40 0.024 UniRef50_Q9SRU7 Cluster: Probable N(2),N(2)-dimethylguanosine tR... 40 0.032 UniRef50_Q24333 Cluster: Elastin like protein; n=1; Drosophila m... 38 0.13 UniRef50_A7AUB1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 37 0.17 UniRef50_Q5C0A4 Cluster: SJCHGC09205 protein; n=1; Schistosoma j... 37 0.22 UniRef50_Q8IEC3 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 36 0.39 UniRef50_Q7QW00 Cluster: GLP_239_44022_45620; n=1; Giardia lambl... 36 0.39 UniRef50_Q0W1J5 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 36 0.39 UniRef50_Q2NI56 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 36 0.39 UniRef50_Q0P6N7 Cluster: Plasma memebrane H+-ATPase; n=1; Planta... 35 0.91 UniRef50_Q4N4W5 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 35 0.91 UniRef50_A5JZI1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 35 0.91 UniRef50_Q9V1P3 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 34 1.2 UniRef50_Q4YVY3 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 34 1.6 UniRef50_Q8SR99 Cluster: N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRAN... 34 1.6 UniRef50_Q589S4 Cluster: HMG protein TCF/LEF; n=1; Dugesia japon... 33 2.8 UniRef50_Q4YAZ4 Cluster: Putative uncharacterized protein; n=1; ... 33 2.8 UniRef50_Q8PU28 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 33 2.8 UniRef50_A7D179 Cluster: tRNA (Guanine-N(2)-)-methyltransferase;... 33 3.7 UniRef50_Q4J947 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 32 4.8 UniRef50_Q01C22 Cluster: TRNA methyltransferase; n=1; Ostreococc... 32 6.4 UniRef50_Q86SD1 Cluster: Calumenin homologue; n=2; Eukaryota|Rep... 32 6.4 UniRef50_P57705 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 32 6.4 UniRef50_Q5CDI1 Cluster: Putative uncharacterized protein; n=1; ... 31 8.5 UniRef50_A5UM08 Cluster: N2,N2-dimethylguanosine tRNA methyltran... 31 8.5 UniRef50_A4YHH9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase;... 31 8.5 UniRef50_Q97ZH0 Cluster: N(2),N(2)-dimethylguanosine tRNA methyl... 31 8.5 >UniRef50_Q6C410 Cluster: Similar to sp|P15565 Saccharomyces cerevisiae TRM1 N; n=1; Yarrowia lipolytica|Rep: Similar to sp|P15565 Saccharomyces cerevisiae TRM1 N - Yarrowia lipolytica (Candida lipolytica) Length = 492 Score = 62.5 bits (145), Expect = 4e-09 Identities = 37/63 (58%), Positives = 46/63 (73%), Gaps = 1/63 (1%) Frame = +2 Query: 260 AEKLARFEKKQKKLETVQDEESRGNPE-PKITILEALSATGLXSIRYAKEIPYATNIIAN 436 AE+ A+ KK+K+ E ++EES G P P+ ILEALSA+GL +IRYAKEIP NIIAN Sbjct: 50 AEEKAKKNKKRKRDEK-ENEES-GKPHIPEFNILEALSASGLRAIRYAKEIPGVKNIIAN 107 Query: 437 DLS 445 D S Sbjct: 108 DFS 110 Score = 41.5 bits (93), Expect = 0.008 Identities = 20/38 (52%), Positives = 26/38 (68%), Gaps = 1/38 (2%) Frame = +3 Query: 141 IKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFIE 251 I EG+A I E +VFYNPVQ+FNRDLS+ + + E Sbjct: 10 ITEGKANILFPKENQVFYNPVQQFNRDLSVTCIRAWSE 47 >UniRef50_A6RDC7 Cluster: Putative uncharacterized protein; n=1; Ajellomyces capsulatus NAm1|Rep: Putative uncharacterized protein - Ajellomyces capsulatus NAm1 Length = 797 Score = 60.5 bits (140), Expect = 2e-08 Identities = 30/60 (50%), Positives = 39/60 (65%) Frame = +2 Query: 266 KLARFEKKQKKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 K R E++ T + N P +IL+ALSATGL ++RYAKEIP+AT I+ANDLS Sbjct: 184 KCVRAEQQDTATGTTTTTVTEQNKIPPFSILDALSATGLRALRYAKEIPFATRIVANDLS 243 Score = 37.9 bits (84), Expect = 0.097 Identities = 14/26 (53%), Positives = 20/26 (76%) Frame = +3 Query: 174 TEKVFYNPVQEFNRDLSIAVLTLFIE 251 T+ VFYNP+Q+FNRDLS+ + + E Sbjct: 103 TQSVFYNPIQQFNRDLSVLAIRAYGE 128 >UniRef50_Q1DMI8 Cluster: Putative uncharacterized protein; n=1; Coccidioides immitis|Rep: Putative uncharacterized protein - Coccidioides immitis Length = 853 Score = 58.0 bits (134), Expect = 9e-08 Identities = 25/35 (71%), Positives = 31/35 (88%) Frame = +2 Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 P TIL+ALSATGL ++RYAKEIP+AT ++ANDLS Sbjct: 296 PSFTILDALSATGLRALRYAKEIPFATQVVANDLS 330 Score = 35.1 bits (77), Expect = 0.69 Identities = 13/23 (56%), Positives = 18/23 (78%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTLFIE 251 VFYNP+Q+FNRDLS+ + + E Sbjct: 194 VFYNPIQQFNRDLSVLAIRAYGE 216 >UniRef50_P15565 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase, mitochondrial precursor (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=9; Saccharomycetales|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase, mitochondrial precursor (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Saccharomyces cerevisiae (Baker's yeast) Length = 570 Score = 56.0 bits (129), Expect = 3e-07 Identities = 30/55 (54%), Positives = 38/55 (69%), Gaps = 3/55 (5%) Frame = +2 Query: 287 KQKKLETVQDEESRGNP---EPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 K++K+ +E+ GN EP I ILEALSATGL +IRYA EIP+ +IANDL Sbjct: 111 KRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVREVIANDL 165 Score = 44.0 bits (99), Expect = 0.001 Identities = 19/39 (48%), Positives = 27/39 (69%), Gaps = 1/39 (2%) Frame = +3 Query: 123 ISNLKTIKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVL 236 I + +KEG+A+I E VFYNP+Q+FNRDLS+ + Sbjct: 39 IEDFNIVKEGKAEILFPKKETVFYNPIQQFNRDLSVTCI 77 >UniRef50_UPI00015B5CEF Cluster: PREDICTED: similar to ENSANGP00000010185; n=1; Nasonia vitripennis|Rep: PREDICTED: similar to ENSANGP00000010185 - Nasonia vitripennis Length = 529 Score = 54.0 bits (124), Expect = 1e-06 Identities = 24/38 (63%), Positives = 32/38 (84%) Frame = +3 Query: 141 IKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254 ++EGQA+I L + VFYNPVQEFNRDLSIAVL+ + ++ Sbjct: 13 LEEGQAKILLEKKNVFYNPVQEFNRDLSIAVLSQYSKE 50 Score = 53.6 bits (123), Expect = 2e-06 Identities = 23/52 (44%), Positives = 37/52 (71%) Frame = +2 Query: 290 QKKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 Q +++ ++++ + + + +LEALSATGL SIRYAKE+P I+AND+S Sbjct: 54 QSSIDSAKEKQKDDSQKTGLRVLEALSATGLRSIRYAKEVPGMAEIVANDIS 105 >UniRef50_Q0UJY7 Cluster: Putative uncharacterized protein; n=1; Phaeosphaeria nodorum|Rep: Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum) Length = 677 Score = 54.0 bits (124), Expect = 1e-06 Identities = 23/35 (65%), Positives = 31/35 (88%) Frame = +2 Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 P +IL+ALSATGL ++RYAKEIP+AT++ AND+S Sbjct: 204 PPFSILDALSATGLRALRYAKEIPFATSVTANDMS 238 Score = 40.3 bits (90), Expect = 0.018 Identities = 15/27 (55%), Positives = 22/27 (81%) Frame = +3 Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIEDI 257 ++VFYNP+Q+FNRDLS+ + F +DI Sbjct: 64 QQVFYNPIQQFNRDLSVLAIKTFGQDI 90 >UniRef50_A7RIG7 Cluster: Predicted protein; n=2; Nematostella vectensis|Rep: Predicted protein - Nematostella vectensis Length = 607 Score = 53.2 bits (122), Expect = 2e-06 Identities = 26/40 (65%), Positives = 31/40 (77%), Gaps = 1/40 (2%) Frame = +3 Query: 141 IKEGQAQICLTTEK-VFYNPVQEFNRDLSIAVLTLFIEDI 257 I+EG+A+I EK VFYNPVQEFNRD+S AV+ L EDI Sbjct: 24 IREGKAEIIFPAEKAVFYNPVQEFNRDISSAVIRLVCEDI 63 Score = 44.8 bits (101), Expect = 8e-04 Identities = 19/34 (55%), Positives = 28/34 (82%) Frame = +2 Query: 344 KITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 ++TILE L+A+GL S+RYA E+P ++IAND+S Sbjct: 93 QVTILEGLAASGLRSVRYALEVPGIHHVIANDIS 126 >UniRef50_Q4WEX8 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase; n=10; Trichocomaceae|Rep: N2,N2-dimethylguanosine tRNA methyltransferase - Aspergillus fumigatus (Sartorya fumigata) Length = 747 Score = 53.2 bits (122), Expect = 2e-06 Identities = 23/35 (65%), Positives = 29/35 (82%) Frame = +2 Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 P TIL+ALSATGL ++RYA EIP+ T ++ANDLS Sbjct: 196 PSFTILDALSATGLRALRYASEIPFTTCVVANDLS 230 Score = 36.7 bits (81), Expect = 0.22 Identities = 13/27 (48%), Positives = 20/27 (74%) Frame = +3 Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIEDI 257 + VFYNP+Q+FNRDLS+ + + E + Sbjct: 112 QSVFYNPIQQFNRDLSVLAIKAYGEHV 138 >UniRef50_A6RZH6 Cluster: Putative uncharacterized protein; n=2; Sclerotiniaceae|Rep: Putative uncharacterized protein - Botryotinia fuckeliana B05.10 Length = 688 Score = 53.2 bits (122), Expect = 2e-06 Identities = 25/38 (65%), Positives = 32/38 (84%), Gaps = 1/38 (2%) Frame = +2 Query: 332 NP-EPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 NP P+ IL+ALSATGL ++RYA+EIP+AT+I ANDL Sbjct: 197 NPINPRFQILDALSATGLRALRYAQEIPFATSITANDL 234 Score = 39.5 bits (88), Expect = 0.032 Identities = 18/41 (43%), Positives = 27/41 (65%) Frame = +3 Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDI 257 +T +GQ Q+ + VFYNP+Q+FNRDLS+ + + E I Sbjct: 46 QTTPKGQNQV----QSVFYNPIQQFNRDLSVLAIKAYGESI 82 >UniRef50_Q5KQ20 Cluster: TRNA (Guanine-N2-)-methyltransferase, putative; n=1; Filobasidiella neoformans|Rep: TRNA (Guanine-N2-)-methyltransferase, putative - Cryptococcus neoformans (Filobasidiella neoformans) Length = 666 Score = 52.4 bits (120), Expect = 4e-06 Identities = 25/35 (71%), Positives = 28/35 (80%) Frame = +2 Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 P I ILEAL+ATGL SIRYAKEIP ++ANDLS Sbjct: 144 PSINILEALAATGLRSIRYAKEIPNVKYVLANDLS 178 Score = 35.5 bits (78), Expect = 0.52 Identities = 16/35 (45%), Positives = 21/35 (60%) Frame = +3 Query: 147 EGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIE 251 E I L E F NPVQ +NRD+S+AV+ + E Sbjct: 24 ESTTTIFLPNEGAFLNPVQHYNRDMSVAVIRAWNE 58 >UniRef50_Q7S9X0 Cluster: Putative uncharacterized protein NCU06336.1; n=2; Sordariales|Rep: Putative uncharacterized protein NCU06336.1 - Neurospora crassa Length = 683 Score = 51.6 bits (118), Expect = 7e-06 Identities = 24/52 (46%), Positives = 35/52 (67%) Frame = +2 Query: 287 KQKKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 +Q E E +G +P T+L+ALSA+GL ++RYA EIP+ T++ ANDL Sbjct: 157 EQGDKEATNGEAKKGK-QPPFTVLDALSASGLRALRYAHEIPFLTSVTANDL 207 Score = 32.7 bits (71), Expect = 3.7 Identities = 10/22 (45%), Positives = 18/22 (81%) Frame = +3 Query: 180 KVFYNPVQEFNRDLSIAVLTLF 245 +VFYNP+Q++NRDL++ + + Sbjct: 56 QVFYNPIQQYNRDLTVLAIKAY 77 >UniRef50_Q54PG3 Cluster: Putative uncharacterized protein; n=1; Dictyostelium discoideum AX4|Rep: Putative uncharacterized protein - Dictyostelium discoideum AX4 Length = 604 Score = 50.8 bits (116), Expect = 1e-05 Identities = 24/44 (54%), Positives = 32/44 (72%), Gaps = 1/44 (2%) Frame = +3 Query: 126 SNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFIED 254 SN+ T+ E A I + +VFYNPVQEFNRD+SI ++ LFIE+ Sbjct: 49 SNIVTVIENSATILYNNQNEVFYNPVQEFNRDMSILMIKLFIEE 92 Score = 46.4 bits (105), Expect = 3e-04 Identities = 25/40 (62%), Positives = 30/40 (75%), Gaps = 1/40 (2%) Frame = +2 Query: 326 RGNPEPKITILEALSATGLXSIRYAKEIPYATN-IIANDL 442 RG P KI +LEAL+ATGL SIRYAKEI + I+AND+ Sbjct: 100 RGKPFKKIRVLEALAATGLRSIRYAKEIGDDLDYILANDI 139 >UniRef50_A1CC48 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase, putative; n=2; Aspergillus|Rep: N2,N2-dimethylguanosine tRNA methyltransferase, putative - Aspergillus clavatus Length = 1045 Score = 50.8 bits (116), Expect = 1e-05 Identities = 25/44 (56%), Positives = 33/44 (75%), Gaps = 3/44 (6%) Frame = +2 Query: 323 SRGNPEPKI---TILEALSATGLXSIRYAKEIPYATNIIANDLS 445 S +PE + TIL+ALSATGL ++RYA EIP+ T ++ANDLS Sbjct: 466 SAPHPEQSVIPFTILDALSATGLRALRYASEIPFVTCVVANDLS 509 Score = 36.7 bits (81), Expect = 0.22 Identities = 13/28 (46%), Positives = 21/28 (75%) Frame = +3 Query: 174 TEKVFYNPVQEFNRDLSIAVLTLFIEDI 257 ++ VFYNP+Q+FNRDLS+ + + E + Sbjct: 391 SQSVFYNPIQQFNRDLSVLAIKAYGEHL 418 >UniRef50_Q386C5 Cluster: N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative; n=3; Trypanosoma|Rep: N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative - Trypanosoma brucei Length = 802 Score = 50.4 bits (115), Expect = 2e-05 Identities = 25/44 (56%), Positives = 32/44 (72%) Frame = +2 Query: 311 QDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 ++ +++G ITILEALSATGL +IRY KEIP IIAND+ Sbjct: 191 EEPKNKGGTRRGITILEALSATGLRAIRYYKEIPDVRFIIANDI 234 Score = 33.1 bits (72), Expect = 2.8 Identities = 13/23 (56%), Positives = 16/23 (69%) Frame = +3 Query: 177 EKVFYNPVQEFNRDLSIAVLTLF 245 + VFYNP Q NRDLS+ V+ F Sbjct: 163 QAVFYNPAQVVNRDLSVCVIACF 185 >UniRef50_Q4P6Q4 Cluster: Putative uncharacterized protein; n=1; Ustilago maydis|Rep: Putative uncharacterized protein - Ustilago maydis (Smut fungus) Length = 711 Score = 50.0 bits (114), Expect = 2e-05 Identities = 24/34 (70%), Positives = 28/34 (82%) Frame = +2 Query: 344 KITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 + T+LEALSATGL SIRYAKEIP ++ANDLS Sbjct: 172 QFTLLEALSATGLRSIRYAKEIPLLRWVLANDLS 205 Score = 33.9 bits (74), Expect = 1.6 Identities = 17/37 (45%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +3 Query: 144 KEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLFIE 251 +E A I + + E F NPVQEFNRDLS + + E Sbjct: 28 RENSATIVMPSAEAAFLNPVQEFNRDLSTLAIRTWSE 64 >UniRef50_Q9P804 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=1; Schizosaccharomyces pombe|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Schizosaccharomyces pombe (Fission yeast) Length = 548 Score = 50.0 bits (114), Expect = 2e-05 Identities = 24/31 (77%), Positives = 26/31 (83%) Frame = +2 Query: 350 TILEALSATGLXSIRYAKEIPYATNIIANDL 442 TILEALSATGL SIRYAKE+P I+ANDL Sbjct: 126 TILEALSATGLRSIRYAKELPNVKRILANDL 156 Score = 38.3 bits (85), Expect = 0.074 Identities = 18/39 (46%), Positives = 26/39 (66%), Gaps = 1/39 (2%) Frame = +3 Query: 138 TIKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVLTLFIE 251 ++ EG A I + +VFYNPVQ+FNRDLS+ + + E Sbjct: 31 SLTEGSAIIPFSNPNEVFYNPVQQFNRDLSVTAIRAWSE 69 >UniRef50_UPI0000D56C3B Cluster: PREDICTED: similar to Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=2; Endopterygota|Rep: PREDICTED: similar to Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Tribolium castaneum Length = 501 Score = 48.4 bits (110), Expect = 7e-05 Identities = 24/33 (72%), Positives = 27/33 (81%) Frame = +2 Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 I+ILEALSATGL SIRYAKE+ IIAND+S Sbjct: 69 ISILEALSATGLRSIRYAKEVKGVKEIIANDIS 101 Score = 47.6 bits (108), Expect = 1e-04 Identities = 25/38 (65%), Positives = 27/38 (71%) Frame = +3 Query: 141 IKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254 I EG A+I T VFYNPVQEFNRDLSI VL F +D Sbjct: 14 ITEGLAKI-KTLGSVFYNPVQEFNRDLSIVVLNTFAKD 50 >UniRef50_Q5C276 Cluster: SJCHGC07232 protein; n=1; Schistosoma japonicum|Rep: SJCHGC07232 protein - Schistosoma japonicum (Blood fluke) Length = 222 Score = 48.0 bits (109), Expect = 9e-05 Identities = 22/41 (53%), Positives = 31/41 (75%) Frame = +3 Query: 123 ISNLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLF 245 ++N +KEG A++ L + VFYNPVQEFNRDL+IA++ F Sbjct: 15 MTNPSFVKEGSAEVMLP-DAVFYNPVQEFNRDLTIAMVNQF 54 Score = 42.7 bits (96), Expect = 0.003 Identities = 20/31 (64%), Positives = 26/31 (83%) Frame = +2 Query: 353 ILEALSATGLXSIRYAKEIPYATNIIANDLS 445 ILEALSA+G+ SIR A E+P ++IIAND+S Sbjct: 82 ILEALSASGIRSIRMALEVPNVSSIIANDIS 112 >UniRef50_Q4QG60 Cluster: N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative; n=3; Leishmania|Rep: N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative - Leishmania major Length = 718 Score = 47.6 bits (108), Expect = 1e-04 Identities = 25/39 (64%), Positives = 27/39 (69%) Frame = +2 Query: 326 RGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 RG ITILEALSATGL +IRY KEI IIAND+ Sbjct: 98 RGGTNEGITILEALSATGLRAIRYYKEITNVRYIIANDM 136 Score = 36.3 bits (80), Expect = 0.30 Identities = 16/35 (45%), Positives = 23/35 (65%) Frame = +3 Query: 141 IKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLF 245 + + +A L + VFYNP Q NRDLSI+V+ +F Sbjct: 53 LSDEEADNQLVDQAVFYNPAQVVNRDLSISVIEVF 87 >UniRef50_UPI000023D9FF Cluster: hypothetical protein FG09705.1; n=1; Gibberella zeae PH-1|Rep: hypothetical protein FG09705.1 - Gibberella zeae PH-1 Length = 1026 Score = 47.2 bits (107), Expect = 2e-04 Identities = 20/36 (55%), Positives = 29/36 (80%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 +P IL+ALSA+GL ++RYA E+P+ T++ ANDLS Sbjct: 133 KPAFRILDALSASGLRALRYAHELPFVTSVKANDLS 168 Score = 35.1 bits (77), Expect = 0.69 Identities = 12/25 (48%), Positives = 20/25 (80%) Frame = +3 Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIE 251 ++VFYNP+Q++NRDLS+ + + E Sbjct: 44 QQVFYNPIQQYNRDLSVLAIKTYGE 68 >UniRef50_Q9NXH9-2 Cluster: Isoform 2 of Q9NXH9 ; n=2; Homo/Pan/Gorilla group|Rep: Isoform 2 of Q9NXH9 - Homo sapiens (Human) Length = 630 Score = 46.8 bits (106), Expect = 2e-04 Identities = 22/37 (59%), Positives = 28/37 (75%), Gaps = 1/37 (2%) Frame = +3 Query: 138 TIKEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLF 245 T+ EG A+I + +VFYNPVQEFNRDL+ AV+T F Sbjct: 56 TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF 92 Score = 39.1 bits (87), Expect = 0.042 Identities = 17/36 (47%), Positives = 26/36 (72%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 E + +LE L+A+GL SIR+A E+P +++AND S Sbjct: 151 EEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDAS 186 >UniRef50_A4RKM5 Cluster: Putative uncharacterized protein; n=1; Magnaporthe grisea|Rep: Putative uncharacterized protein - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) Length = 702 Score = 46.8 bits (106), Expect = 2e-04 Identities = 19/34 (55%), Positives = 28/34 (82%) Frame = +2 Query: 341 PKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 P++ IL+ALSATGL ++RYA E+P+ T + +NDL Sbjct: 196 PRLKILDALSATGLRALRYAHELPFPTMVTSNDL 229 Score = 35.1 bits (77), Expect = 0.69 Identities = 12/23 (52%), Positives = 18/23 (78%) Frame = +3 Query: 177 EKVFYNPVQEFNRDLSIAVLTLF 245 + VFYNP+Q+FNRDLS+ + + Sbjct: 69 QTVFYNPIQQFNRDLSVLAIKAY 91 >UniRef50_Q9NXH9 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=17; Euteleostomi|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Homo sapiens (Human) Length = 659 Score = 46.8 bits (106), Expect = 2e-04 Identities = 22/37 (59%), Positives = 28/37 (75%), Gaps = 1/37 (2%) Frame = +3 Query: 138 TIKEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLF 245 T+ EG A+I + +VFYNPVQEFNRDL+ AV+T F Sbjct: 56 TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF 92 Score = 39.1 bits (87), Expect = 0.042 Identities = 17/36 (47%), Positives = 26/36 (72%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 E + +LE L+A+GL SIR+A E+P +++AND S Sbjct: 151 EEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDAS 186 >UniRef50_A6QP09 Cluster: LOC539613 protein; n=2; Bos taurus|Rep: LOC539613 protein - Bos taurus (Bovine) Length = 691 Score = 46.4 bits (105), Expect = 3e-04 Identities = 23/37 (62%), Positives = 27/37 (72%), Gaps = 1/37 (2%) Frame = +3 Query: 138 TIKEGQAQICL-TTEKVFYNPVQEFNRDLSIAVLTLF 245 TI EG A+I +VFYNPVQEFNRDL+ AV+T F Sbjct: 87 TITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEF 123 Score = 41.1 bits (92), Expect = 0.010 Identities = 17/36 (47%), Positives = 27/36 (75%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 E + +LE L+A+GL SIR+A+E+P +++AND S Sbjct: 182 EEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDAS 217 >UniRef50_Q23270 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=2; Caenorhabditis|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Caenorhabditis elegans Length = 526 Score = 46.4 bits (105), Expect = 3e-04 Identities = 24/62 (38%), Positives = 42/62 (67%), Gaps = 5/62 (8%) Frame = +2 Query: 275 RFEKKQKKLETVQDEESRGNP-----EPKITILEALSATGLXSIRYAKEIPYATNIIAND 439 ++ ++QK+L+T ++ + N + KI IL+ALSA+GL ++R++KE+P I+AND Sbjct: 63 KWAEEQKQLKTEEEPPKKKNKLAINEDGKIRILDALSASGLRALRFSKEVPNVGFIMAND 122 Query: 440 LS 445 S Sbjct: 123 FS 124 Score = 45.2 bits (102), Expect = 6e-04 Identities = 23/49 (46%), Positives = 31/49 (63%) Frame = +3 Query: 108 MRMETISNLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254 ++ E + I+EGQA++ VFYNPVQEFNRDL++ VL F D Sbjct: 13 IKSEDKEEVTVIQEGQAKVGFHGP-VFYNPVQEFNRDLTVTVLRQFSAD 60 >UniRef50_Q9VK89 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=4; Diptera|Rep: Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Drosophila melanogaster (Fruit fly) Length = 578 Score = 46.0 bits (104), Expect = 4e-04 Identities = 29/67 (43%), Positives = 41/67 (61%), Gaps = 12/67 (17%) Frame = +2 Query: 281 EKKQKKLETVQDEESRGNPEPK------------ITILEALSATGLXSIRYAKEIPYATN 424 +K++KK++ +DE++ PE + ILEAL+ATGL SIRYA+EI Sbjct: 67 KKQRKKVKEQEDEKTTPVPEDPPVYEAGTRYEDGLRILEALAATGLRSIRYAQEIAGVRQ 126 Query: 425 IIANDLS 445 I+ANDLS Sbjct: 127 IVANDLS 133 Score = 44.4 bits (100), Expect = 0.001 Identities = 22/39 (56%), Positives = 28/39 (71%) Frame = +3 Query: 129 NLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLF 245 N I+E A+I ++ VFYNPVQEFNRDLSIA L ++ Sbjct: 16 NENVIRERNAEI-VSGGNVFYNPVQEFNRDLSIAALNVY 53 >UniRef50_UPI0000E48B85 Cluster: PREDICTED: hypothetical protein; n=2; Strongylocentrotus purpuratus|Rep: PREDICTED: hypothetical protein - Strongylocentrotus purpuratus Length = 556 Score = 45.2 bits (102), Expect = 6e-04 Identities = 21/39 (53%), Positives = 28/39 (71%), Gaps = 1/39 (2%) Frame = +3 Query: 141 IKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVLTLFIED 254 ++EG+A+I +VFYNP Q NRDLS+AV LF+ED Sbjct: 13 VEEGRARIVFPNPNEVFYNPAQVINRDLSLAVTGLFVED 51 Score = 45.2 bits (102), Expect = 6e-04 Identities = 21/36 (58%), Positives = 26/36 (72%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 E IT LEALSA+GL S+R AKE+P ++AND S Sbjct: 92 EEGITFLEALSASGLRSVRIAKEVPGIKRVVANDFS 127 >UniRef50_UPI00004990C0 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase; n=1; Entamoeba histolytica HM-1:IMSS|Rep: N2,N2-dimethylguanosine tRNA methyltransferase - Entamoeba histolytica HM-1:IMSS Length = 489 Score = 45.2 bits (102), Expect = 6e-04 Identities = 21/45 (46%), Positives = 30/45 (66%), Gaps = 2/45 (4%) Frame = +3 Query: 126 SNLKTIKEGQAQICLTTEKV--FYNPVQEFNRDLSIAVLTLFIED 254 S IKEGQA + E+ FYNPVQE NRDLSI++++ ++ + Sbjct: 7 SKYTPIKEGQATVLFPKEEAGAFYNPVQELNRDLSISIISKYLRN 51 Score = 42.7 bits (96), Expect = 0.003 Identities = 23/53 (43%), Positives = 35/53 (66%), Gaps = 2/53 (3%) Frame = +2 Query: 293 KKLETVQDEESRGNPEPKITILEALSATGLXSIRYAKEIPYATN--IIANDLS 445 K L ++E + N + I+EAL+A+GL S+RYAKE+P + +IAND+S Sbjct: 47 KYLRNKENELKQKNKQYHPLIVEALAASGLRSVRYAKELPQDIDFKVIANDIS 99 >UniRef50_A0CLP3 Cluster: Chromosome undetermined scaffold_20, whole genome shotgun sequence; n=1; Paramecium tetraurelia|Rep: Chromosome undetermined scaffold_20, whole genome shotgun sequence - Paramecium tetraurelia Length = 487 Score = 44.0 bits (99), Expect = 0.001 Identities = 23/38 (60%), Positives = 29/38 (76%) Frame = +2 Query: 332 NPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 N +P ITIL+ALSA+GL S+R+A+EI I ANDLS Sbjct: 67 NRQP-ITILDALSASGLRSVRFAQEIQNVKEIYANDLS 103 Score = 35.1 bits (77), Expect = 0.69 Identities = 15/29 (51%), Positives = 20/29 (68%) Frame = +3 Query: 168 LTTEKVFYNPVQEFNRDLSIAVLTLFIED 254 L E+VFYNP Q NRDL++ V+ F E+ Sbjct: 39 LIEEEVFYNPAQIINRDLTVLVIDTFQEN 67 >UniRef50_UPI000155D1C2 Cluster: PREDICTED: similar to TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae); n=1; Ornithorhynchus anatinus|Rep: PREDICTED: similar to TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) - Ornithorhynchus anatinus Length = 223 Score = 43.2 bits (97), Expect = 0.003 Identities = 21/36 (58%), Positives = 26/36 (72%), Gaps = 1/36 (2%) Frame = +3 Query: 141 IKEGQAQICLT-TEKVFYNPVQEFNRDLSIAVLTLF 245 + EG A+I +VFYNPVQEFNRDL+ AV+T F Sbjct: 59 VTEGAARIVFPGANEVFYNPVQEFNRDLTCAVVTEF 94 >UniRef50_Q5A863 Cluster: Likely N2,N2-dimethylguanosine-specific tRNA methyltransferase; n=5; Saccharomycetales|Rep: Likely N2,N2-dimethylguanosine-specific tRNA methyltransferase - Candida albicans (Yeast) Length = 556 Score = 43.2 bits (97), Expect = 0.003 Identities = 22/46 (47%), Positives = 31/46 (67%), Gaps = 2/46 (4%) Frame = +3 Query: 120 TISN-LKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFIE 251 TIS T++EG+A I + +VFYNP+Q+FNRDLSI + + E Sbjct: 35 TISQEFNTVQEGKATILTPKQDEVFYNPIQQFNRDLSIMAIKAYDE 80 Score = 40.3 bits (90), Expect = 0.018 Identities = 20/55 (36%), Positives = 34/55 (61%), Gaps = 1/55 (1%) Frame = +2 Query: 281 EKKQKKLETVQDEESRGNPEPK-ITILEALSATGLXSIRYAKEIPYATNIIANDL 442 E + +K++ ++ + + + ILE+L+A+GL S RY EIP A I+AND+ Sbjct: 80 EIRHEKIQAIKKKSKNKRTKLNGLKILESLAASGLRSCRYGLEIPEAGKIVANDM 134 >UniRef50_Q5CQU6 Cluster: Trm1p. N2,N2-dimethylguanosine tRNA methyltransferase; n=2; Cryptosporidium|Rep: Trm1p. N2,N2-dimethylguanosine tRNA methyltransferase - Cryptosporidium parvum Iowa II Length = 637 Score = 42.7 bits (96), Expect = 0.003 Identities = 18/41 (43%), Positives = 28/41 (68%) Frame = +3 Query: 126 SNLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFI 248 S+ + I EG+ +I T + +FYNP Q FNRD+S+ V+ F+ Sbjct: 15 SSSEYIHEGKVKIHKTNDDIFYNPAQVFNRDISLIVIKSFL 55 Score = 33.5 bits (73), Expect = 2.1 Identities = 15/39 (38%), Positives = 25/39 (64%), Gaps = 1/39 (2%) Frame = +2 Query: 329 GNPEPKITILEALSATGLXSIRYAKEIPY-ATNIIANDL 442 G+ +T+LE L A+GL SIRY KE+ + +++ D+ Sbjct: 104 GSSRFSVTVLEPLGASGLRSIRYIKELSHEIDHVVCGDI 142 >UniRef50_Q22HL7 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase family protein; n=1; Tetrahymena thermophila SB210|Rep: N2,N2-dimethylguanosine tRNA methyltransferase family protein - Tetrahymena thermophila SB210 Length = 557 Score = 41.1 bits (92), Expect = 0.010 Identities = 20/43 (46%), Positives = 30/43 (69%) Frame = +2 Query: 317 EESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 +E+R ITIL+ALSA+GL +IR++KE+ + AND+S Sbjct: 91 KETRKEKFDGITILDALSASGLRAIRFSKELKDIKKVYANDIS 133 Score = 37.1 bits (82), Expect = 0.17 Identities = 15/24 (62%), Positives = 20/24 (83%) Frame = +3 Query: 180 KVFYNPVQEFNRDLSIAVLTLFIE 251 +VFYNPVQ FNRDL++ V+ +F E Sbjct: 65 EVFYNPVQVFNRDLTVLVIHMFKE 88 >UniRef50_A2FDH4 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase family protein; n=4; Trichomonas vaginalis G3|Rep: N2,N2-dimethylguanosine tRNA methyltransferase family protein - Trichomonas vaginalis G3 Length = 476 Score = 41.1 bits (92), Expect = 0.010 Identities = 21/39 (53%), Positives = 26/39 (66%) Frame = +2 Query: 326 RGNPEPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 R + I+I EA +A+GL SIRYAKEI II+NDL Sbjct: 55 RNSVRDDISIFEAFAASGLRSIRYAKEITGIKEIISNDL 93 Score = 35.9 bits (79), Expect = 0.39 Identities = 14/21 (66%), Positives = 18/21 (85%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTLF 245 VFYNPVQ+FNRDL+ AV+ + Sbjct: 34 VFYNPVQQFNRDLTCAVIQAY 54 >UniRef50_Q8RU67 Cluster: Putative tRNA (Guanine-N2-)-Methyltransferase; n=3; Oryza sativa|Rep: Putative tRNA (Guanine-N2-)-Methyltransferase - Oryza sativa (Rice) Length = 490 Score = 39.9 bits (89), Expect = 0.024 Identities = 22/38 (57%), Positives = 26/38 (68%), Gaps = 1/38 (2%) Frame = +3 Query: 138 TIKEGQAQICLT-TEKVFYNPVQEFNRDLSIAVLTLFI 248 TI+EG+A+I + VFYN Q NRDLSIAVL FI Sbjct: 53 TIREGRAEIFADDSNSVFYNKAQVNNRDLSIAVLRSFI 90 Score = 31.9 bits (69), Expect = 6.4 Identities = 15/29 (51%), Positives = 21/29 (72%) Frame = +2 Query: 353 ILEALSATGLXSIRYAKEIPYATNIIAND 439 +LEAL+A+GL +IRYA E+ +IA D Sbjct: 141 VLEALAASGLRAIRYALEVDGIGEVIAVD 169 >UniRef50_Q339G6 Cluster: N-dimethylguanosine tRNA methyltransferase, putative, expressed; n=2; Oryza sativa (japonica cultivar-group)|Rep: N-dimethylguanosine tRNA methyltransferase, putative, expressed - Oryza sativa subsp. japonica (Rice) Length = 555 Score = 39.9 bits (89), Expect = 0.024 Identities = 22/38 (57%), Positives = 26/38 (68%), Gaps = 1/38 (2%) Frame = +3 Query: 138 TIKEGQAQICLT-TEKVFYNPVQEFNRDLSIAVLTLFI 248 TI+EG+A+I + VFYN Q NRDLSIAVL FI Sbjct: 53 TIREGRAEIFADDSNSVFYNKAQVNNRDLSIAVLRSFI 90 Score = 31.9 bits (69), Expect = 6.4 Identities = 15/29 (51%), Positives = 21/29 (72%) Frame = +2 Query: 353 ILEALSATGLXSIRYAKEIPYATNIIAND 439 +LEAL+A+GL +IRYA E+ +IA D Sbjct: 141 VLEALAASGLRAIRYALEVDGIGEVIAVD 169 >UniRef50_Q9SRU7 Cluster: Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=13; Viridiplantae|Rep: Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Arabidopsis thaliana (Mouse-ear cress) Length = 599 Score = 39.5 bits (88), Expect = 0.032 Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 2/47 (4%) Frame = +3 Query: 114 MET-ISNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFI 248 MET +++ IKEG+A+I + + +VF+N Q NRD+SIAVL F+ Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFL 47 Score = 33.1 bits (72), Expect = 2.8 Identities = 17/35 (48%), Positives = 25/35 (71%), Gaps = 2/35 (5%) Frame = +2 Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIA--NDLS 445 I I +ALSA+GL ++RYA+EI ++A NDL+ Sbjct: 132 ILIFQALSASGLRALRYAREIEGIGQVVALDNDLA 166 >UniRef50_Q24333 Cluster: Elastin like protein; n=1; Drosophila melanogaster|Rep: Elastin like protein - Drosophila melanogaster (Fruit fly) Length = 110 Score = 37.5 bits (83), Expect = 0.13 Identities = 25/54 (46%), Positives = 28/54 (51%), Gaps = 7/54 (12%) Frame = +2 Query: 29 TAXXXXLELVDPPGCRNSA----RAHF-TDLYENGNY--IKLKNYKRRPSPDMF 169 TA LELVDPPGCRNSA RA+ + LY Y + L Y P P F Sbjct: 4 TAVAAALELVDPPGCRNSARDRQRANLNSQLYAGYPYPGVYLPQYPAYPQPAQF 57 >UniRef50_A7AUB1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative; n=1; Babesia bovis|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative - Babesia bovis Length = 544 Score = 37.1 bits (82), Expect = 0.17 Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 3/41 (7%) Frame = +3 Query: 138 TIKEGQ---AQICLTTEKVFYNPVQEFNRDLSIAVLTLFIE 251 T+ EG + + + + +FYNP Q FNRDLS+ VL F+E Sbjct: 6 TVTEGLVTVSSLSVDGQPLFYNPPQVFNRDLSLIVLKTFVE 46 Score = 35.9 bits (79), Expect = 0.39 Identities = 16/33 (48%), Positives = 23/33 (69%), Gaps = 1/33 (3%) Frame = +2 Query: 347 ITILEALSATGLXSIRYAKEI-PYATNIIANDL 442 + +LE L+ATG+ SIRY KE+ Y ++ NDL Sbjct: 70 VNVLEMLAATGIRSIRYLKELGDYVNHVYINDL 102 >UniRef50_Q5C0A4 Cluster: SJCHGC09205 protein; n=1; Schistosoma japonicum|Rep: SJCHGC09205 protein - Schistosoma japonicum (Blood fluke) Length = 215 Score = 36.7 bits (81), Expect = 0.22 Identities = 16/20 (80%), Positives = 16/20 (80%) Frame = +2 Query: 29 TAXXXXLELVDPPGCRNSAR 88 TA LELVDPPGCRNSAR Sbjct: 5 TAVAAALELVDPPGCRNSAR 24 >UniRef50_Q8IEC3 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase, putative; n=2; Plasmodium|Rep: N2,N2-dimethylguanosine tRNA methyltransferase, putative - Plasmodium falciparum (isolate 3D7) Length = 804 Score = 35.9 bits (79), Expect = 0.39 Identities = 22/54 (40%), Positives = 31/54 (57%), Gaps = 4/54 (7%) Frame = +2 Query: 293 KKLETVQDEESRGNPE---PKITILEALSATGLXSIRYAKEIPYATN-IIANDL 442 K LE E+++ N + I+E LSA+G+ SIRYAKE+ N I ND+ Sbjct: 85 KALEIFMKEKNKNNEKIIFRGFNIIELLSASGMRSIRYAKELEDTINHITTNDI 138 Score = 33.1 bits (72), Expect = 2.8 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = +3 Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVL 236 K I EG +I +FYN Q FNRD+SI ++ Sbjct: 51 KFIYEGSVKIKNKKNHIFYNKAQVFNRDMSIVLI 84 >UniRef50_Q7QW00 Cluster: GLP_239_44022_45620; n=1; Giardia lamblia ATCC 50803|Rep: GLP_239_44022_45620 - Giardia lamblia ATCC 50803 Length = 532 Score = 35.9 bits (79), Expect = 0.39 Identities = 15/40 (37%), Positives = 23/40 (57%) Frame = +3 Query: 138 TIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDI 257 T EG +VF+NP Q FNRD+S+ V+ +++ I Sbjct: 20 THTEGTVSFTYNPSQVFFNPTQIFNRDISLLVVDQYLQHI 59 >UniRef50_Q0W1J5 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase; n=1; uncultured methanogenic archaeon RC-I|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase - Uncultured methanogenic archaeon RC-I Length = 365 Score = 35.9 bits (79), Expect = 0.39 Identities = 18/36 (50%), Positives = 24/36 (66%) Frame = +3 Query: 132 LKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLT 239 + I+EG+ I + E VFYNP E NRDL++A LT Sbjct: 1 MAVIREGKVAIEMG-EGVFYNPRMEMNRDLNVACLT 35 >UniRef50_Q2NI56 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=2; Methanobacteriaceae|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Methanosphaera stadtmanae (strain DSM 3091) Length = 390 Score = 35.9 bits (79), Expect = 0.39 Identities = 13/25 (52%), Positives = 20/25 (80%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTLFIEDI 257 VFYNPV E NRD+S+AV+ + +++ Sbjct: 27 VFYNPVMEMNRDISVAVINQYRKEV 51 >UniRef50_Q0P6N7 Cluster: Plasma memebrane H+-ATPase; n=1; Plantago major|Rep: Plasma memebrane H+-ATPase - Plantago major (Common plantain) Length = 106 Score = 34.7 bits (76), Expect = 0.91 Identities = 14/14 (100%), Positives = 14/14 (100%) Frame = +2 Query: 47 LELVDPPGCRNSAR 88 LELVDPPGCRNSAR Sbjct: 11 LELVDPPGCRNSAR 24 >UniRef50_Q4N4W5 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase, putative; n=2; Theileria|Rep: N2,N2-dimethylguanosine tRNA methyltransferase, putative - Theileria parva Length = 599 Score = 34.7 bits (76), Expect = 0.91 Identities = 15/22 (68%), Positives = 17/22 (77%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTLFI 248 +FYNP Q FNRDLS+ VL FI Sbjct: 43 LFYNPPQVFNRDLSLLVLKTFI 64 Score = 31.9 bits (69), Expect = 6.4 Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%) Frame = +2 Query: 347 ITILEALSATGLXSIRYAKEI-PYATNIIANDL 442 + ILE+L+ATG+ IRY +E+ P + NDL Sbjct: 83 VNILESLAATGIRGIRYLRELGPLVGMVTFNDL 115 >UniRef50_A5JZI1 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative; n=2; Plasmodium|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative - Plasmodium vivax Length = 949 Score = 34.7 bits (76), Expect = 0.91 Identities = 16/36 (44%), Positives = 22/36 (61%) Frame = +3 Query: 129 NLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVL 236 N K I EG +I + +FYN Q FNRD+SI ++ Sbjct: 67 NSKYIFEGSVKIKNKSNHIFYNKAQVFNRDMSIILI 102 Score = 34.7 bits (76), Expect = 0.91 Identities = 17/31 (54%), Positives = 23/31 (74%), Gaps = 1/31 (3%) Frame = +2 Query: 353 ILEALSATGLXSIRYAKEIPYATN-IIANDL 442 ++E LSA+G+ SIRY KE+ N IIAND+ Sbjct: 126 VVELLSASGIRSIRYVKELRETINHIIANDI 156 >UniRef50_Q9V1P3 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=6; Thermococcaceae|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Pyrococcus abyssi Length = 383 Score = 34.3 bits (75), Expect = 1.2 Identities = 19/36 (52%), Positives = 26/36 (72%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 +P+I +L+ALSATG+ IR+A E P A I ND+S Sbjct: 51 KPRI-VLDALSATGIRGIRFALETP-AEEIWMNDIS 84 >UniRef50_Q4YVY3 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase, putative; n=3; Plasmodium (Vinckeia)|Rep: N2,N2-dimethylguanosine tRNA methyltransferase, putative - Plasmodium berghei Length = 811 Score = 33.9 bits (74), Expect = 1.6 Identities = 16/36 (44%), Positives = 22/36 (61%) Frame = +3 Query: 129 NLKTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVL 236 N K I EG +I + +FYN Q FNRD+SI ++ Sbjct: 52 NNKHIYEGCVKIKNKNKHIFYNKAQVFNRDMSIVLI 87 Score = 33.5 bits (73), Expect = 2.1 Identities = 15/31 (48%), Positives = 22/31 (70%), Gaps = 1/31 (3%) Frame = +2 Query: 353 ILEALSATGLXSIRYAKEIPYATN-IIANDL 442 ++E LSA+G+ SIRY KE+ N I+ ND+ Sbjct: 111 VIELLSASGIRSIRYVKELKETINHIVTNDI 141 >UniRef50_Q8SR99 Cluster: N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRANSFERASE; n=1; Encephalitozoon cuniculi|Rep: N2,N2-DIMETHYLGUANOSINE tRNA METHYLTRANSFERASE - Encephalitozoon cuniculi Length = 432 Score = 33.9 bits (74), Expect = 1.6 Identities = 16/40 (40%), Positives = 25/40 (62%) Frame = +3 Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTLFIED 254 +TI E ++ + E F+NP Q+FNRD+SI V+ +D Sbjct: 9 ETISE-ESTVITKNEWTFFNPAQKFNRDISIEVVKECFKD 47 Score = 33.5 bits (73), Expect = 2.1 Identities = 17/33 (51%), Positives = 23/33 (69%) Frame = +2 Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIANDLS 445 I +L+A+SATGL IRY KEI + + ND+S Sbjct: 51 IRVLDAMSATGLRGIRYLKEIGNSI-VYLNDIS 82 >UniRef50_Q589S4 Cluster: HMG protein TCF/LEF; n=1; Dugesia japonica|Rep: HMG protein TCF/LEF - Dugesia japonica (Planarian) Length = 263 Score = 33.1 bits (72), Expect = 2.8 Identities = 13/17 (76%), Positives = 14/17 (82%) Frame = +2 Query: 50 ELVDPPGCRNSARAHFT 100 +LVDPPGCRNSAR T Sbjct: 5 QLVDPPGCRNSARGECT 21 >UniRef50_Q4YAZ4 Cluster: Putative uncharacterized protein; n=1; Plasmodium berghei|Rep: Putative uncharacterized protein - Plasmodium berghei Length = 89 Score = 33.1 bits (72), Expect = 2.8 Identities = 14/18 (77%), Positives = 14/18 (77%) Frame = +2 Query: 29 TAXXXXLELVDPPGCRNS 82 TA LELVDPPGCRNS Sbjct: 11 TAVAAALELVDPPGCRNS 28 >UniRef50_Q8PU28 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=5; Methanosarcinaceae|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Methanosarcina mazei (Methanosarcina frisia) Length = 388 Score = 33.1 bits (72), Expect = 2.8 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTLFIE 251 VFYNP E NRD+++A F+E Sbjct: 31 VFYNPEMELNRDINVAATAAFVE 53 >UniRef50_A7D179 Cluster: tRNA (Guanine-N(2)-)-methyltransferase; n=1; Halorubrum lacusprofundi ATCC 49239|Rep: tRNA (Guanine-N(2)-)-methyltransferase - Halorubrum lacusprofundi ATCC 49239 Length = 433 Score = 32.7 bits (71), Expect = 3.7 Identities = 12/21 (57%), Positives = 17/21 (80%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTLF 245 VF+NP QE NRD+++AVL + Sbjct: 79 VFFNPTQELNRDVTVAVLRAY 99 >UniRef50_Q4J947 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase; n=1; Sulfolobus acidocaldarius|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase - Sulfolobus acidocaldarius Length = 382 Score = 32.3 bits (70), Expect = 4.8 Identities = 16/35 (45%), Positives = 23/35 (65%) Frame = +2 Query: 338 EPKITILEALSATGLXSIRYAKEIPYATNIIANDL 442 +PK ++++ LSATG+ IRY EI II ND+ Sbjct: 55 KPK-SLIDGLSATGVRGIRYGLEINGVEEIILNDI 88 >UniRef50_Q01C22 Cluster: TRNA methyltransferase; n=1; Ostreococcus tauri|Rep: TRNA methyltransferase - Ostreococcus tauri Length = 123 Score = 31.9 bits (69), Expect = 6.4 Identities = 17/37 (45%), Positives = 23/37 (62%), Gaps = 2/37 (5%) Frame = +3 Query: 141 IKEGQAQIC--LTTEKVFYNPVQEFNRDLSIAVLTLF 245 I+EG A+ T+ VFYN Q NRD+S+AV+ F Sbjct: 19 IREGAARALQRAETDDVFYNKPQVVNRDMSLAVIREF 55 >UniRef50_Q86SD1 Cluster: Calumenin homologue; n=2; Eukaryota|Rep: Calumenin homologue - Ciona intestinalis (Transparent sea squirt) Length = 308 Score = 31.9 bits (69), Expect = 6.4 Identities = 18/45 (40%), Positives = 22/45 (48%) Frame = +2 Query: 29 TAXXXXLELVDPPGCRNSARAHFTDLYENGNYIKLKNYKRRPSPD 163 TA LELVDPPGCR T+ Y + K +KR S + Sbjct: 124 TAVAAALELVDPPGCRLVHEVDETEDYRKMYEREEKRWKRADSDE 168 >UniRef50_P57705 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=4; Halobacteriaceae|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Halobacterium salinarium (Halobacterium halobium) Length = 371 Score = 31.9 bits (69), Expect = 6.4 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = +3 Query: 177 EKVFYNPVQEFNRDLSIAVLTLFIE 251 + VF+N QE NRDL++A L F E Sbjct: 24 DDVFFNATQELNRDLTVATLAAFRE 48 >UniRef50_Q5CDI1 Cluster: Putative uncharacterized protein; n=1; Cryptosporidium hominis|Rep: Putative uncharacterized protein - Cryptosporidium hominis Length = 72 Score = 31.5 bits (68), Expect = 8.5 Identities = 15/23 (65%), Positives = 16/23 (69%) Frame = -2 Query: 96 KWARAEFLQPGGSTSSRXXXXAV 28 K + EFLQPGGSTSSR AV Sbjct: 39 KCEKIEFLQPGGSTSSRAAATAV 61 >UniRef50_A5UM08 Cluster: N2,N2-dimethylguanosine tRNA methyltransferase, Trm1; n=1; Methanobrevibacter smithii ATCC 35061|Rep: N2,N2-dimethylguanosine tRNA methyltransferase, Trm1 - Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) Length = 388 Score = 31.5 bits (68), Expect = 8.5 Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 7/45 (15%) Frame = +3 Query: 132 LKTIKEGQAQICL-------TTEKVFYNPVQEFNRDLSIAVLTLF 245 +KT++EG +I + VFYNP E NRD+SI L F Sbjct: 6 IKTVEEGLTKIQFPEFDKISSDAPVFYNPHMELNRDISILALQTF 50 >UniRef50_A4YHH9 Cluster: TRNA (Guanine-N(2)-)-methyltransferase; n=1; Metallosphaera sedula DSM 5348|Rep: TRNA (Guanine-N(2)-)-methyltransferase - Metallosphaera sedula DSM 5348 Length = 382 Score = 31.5 bits (68), Expect = 8.5 Identities = 13/30 (43%), Positives = 20/30 (66%) Frame = +2 Query: 350 TILEALSATGLXSIRYAKEIPYATNIIAND 439 ++L+A+SATG+ IRY KE ++ ND Sbjct: 58 SVLDAMSATGVRGIRYVKESGVKGEVLFND 87 >UniRef50_Q97ZH0 Cluster: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase); n=2; Sulfolobus|Rep: N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2- dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) - Sulfolobus solfataricus Length = 378 Score = 31.5 bits (68), Expect = 8.5 Identities = 12/20 (60%), Positives = 16/20 (80%) Frame = +3 Query: 183 VFYNPVQEFNRDLSIAVLTL 242 VFYNP FNRDLS+ V+++ Sbjct: 34 VFYNPKMTFNRDLSVIVVSI 53 Database: uniref50 Posted date: Oct 5, 2007 11:19 AM Number of letters in database: 575,637,011 Number of sequences in database: 1,657,284 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 316,864,154 Number of Sequences: 1657284 Number of extensions: 4605538 Number of successful extensions: 14531 Number of sequences better than 10.0: 62 Number of HSP's better than 10.0 without gapping: 14107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 14518 length of database: 575,637,011 effective HSP length: 93 effective length of database: 421,509,599 effective search space used: 22761518346 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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