Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20235
(445 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera... 50 2e-07
SPCC1259.08 |||conserved fungal protein|Schizosaccharomyces pomb... 27 0.98
SPBPB2B2.17c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual 27 1.7
SPAC977.02 |||S. pombe specific 5Tm protein family|Schizosacchar... 27 1.7
SPBC1348.03 |||dubious|Schizosaccharomyces pombe|chr 2|||Manual 27 1.7
SPAC750.04c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 27 1.7
SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schi... 26 2.3
SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po... 25 6.9
SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces p... 25 6.9
SPCC4G3.04c |coq5||C-methytransferase |Schizosaccharomyces pombe... 25 6.9
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 24 9.1
SPAC3C7.02c |||protein kinase inhibitor |Schizosaccharomyces pom... 24 9.1
>SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 50.0 bits (114), Expect = 2e-07
Identities = 24/31 (77%), Positives = 26/31 (83%)
Frame = +2
Query: 350 TILEALSATGLXSIRYAKEIPYATNIIANDL 442
TILEALSATGL SIRYAKE+P I+ANDL
Sbjct: 126 TILEALSATGLRSIRYAKELPNVKRILANDL 156
Score = 38.3 bits (85), Expect = 5e-04
Identities = 18/39 (46%), Positives = 26/39 (66%), Gaps = 1/39 (2%)
Frame = +3
Query: 138 TIKEGQAQICLTT-EKVFYNPVQEFNRDLSIAVLTLFIE 251
++ EG A I + +VFYNPVQ+FNRDLS+ + + E
Sbjct: 31 SLTEGSAIIPFSNPNEVFYNPVQQFNRDLSVTAIRAWSE 69
>SPCC1259.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 394
Score = 27.5 bits (58), Expect = 0.98
Identities = 9/29 (31%), Positives = 18/29 (62%)
Frame = +2
Query: 263 EKLARFEKKQKKLETVQDEESRGNPEPKI 349
E++ F +K++T+ + RG+P+P I
Sbjct: 187 EEIESFNSFSRKMQTISNSRYRGSPKPNI 215
>SPBPB2B2.17c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
Length = 146
Score = 26.6 bits (56), Expect = 1.7
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +3
Query: 78 IRHEPILPIFMRMETISNLKTIKEGQAQICLTTEKV 185
+ H P F+R +I++++T + Q I + EKV
Sbjct: 79 VYHNKFFPRFIRTHSINSIRTFSKFQVIILFSIEKV 114
>SPAC977.02 |||S. pombe specific 5Tm protein
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 146
Score = 26.6 bits (56), Expect = 1.7
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +3
Query: 78 IRHEPILPIFMRMETISNLKTIKEGQAQICLTTEKV 185
+ H P F+R +I++++T + Q I + EKV
Sbjct: 79 VYHNKFFPRFIRTHSINSIRTFSKFQVIILFSIEKV 114
>SPBC1348.03 |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
Length = 146
Score = 26.6 bits (56), Expect = 1.7
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +3
Query: 78 IRHEPILPIFMRMETISNLKTIKEGQAQICLTTEKV 185
+ H P F+R +I++++T + Q I + EKV
Sbjct: 79 VYHNKFFPRFIRTHSINSIRTFSKFQVIILFSIEKV 114
>SPAC750.04c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 146
Score = 26.6 bits (56), Expect = 1.7
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +3
Query: 78 IRHEPILPIFMRMETISNLKTIKEGQAQICLTTEKV 185
+ H P F+R +I++++T + Q I + EKV
Sbjct: 79 VYHNKFFPRFIRTHSINSIRTFSKFQVIILFSIEKV 114
>SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 970
Score = 26.2 bits (55), Expect = 2.3
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 56 VDPPGCRNSARAHFTDLYENGNY 124
+DPPGC++ A + NGNY
Sbjct: 507 IDPPGCQDIDDALHACVLPNGNY 529
>SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 351
Score = 24.6 bits (51), Expect = 6.9
Identities = 12/27 (44%), Positives = 13/27 (48%)
Frame = -2
Query: 90 ARAEFLQPGGSTSSRXXXXAVXKXIKK 10
A+AEF PGGS R V KK
Sbjct: 42 AKAEFQNPGGSVKDRAAYYVVRDAEKK 68
>SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1028
Score = 24.6 bits (51), Expect = 6.9
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = +3
Query: 117 ETISNLKTIKEGQAQICLTTEKVFYNP 197
++ S++K+ KE A L+TE+ FY+P
Sbjct: 87 DSASSIKSSKEDIACRKLSTERFFYSP 113
>SPCC4G3.04c |coq5||C-methytransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 305
Score = 24.6 bits (51), Expect = 6.9
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +3
Query: 135 KTIKEGQAQICLTTEKVFYNPVQEFNRDLSIAVLTL 242
+ +K G CL KV+ P+ E R S +L L
Sbjct: 209 RVLKPGGVFSCLEFSKVYPAPLAELYRQYSFKILPL 244
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 24.2 bits (50), Expect = 9.1
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +2
Query: 251 RYKAEKLARFEKKQKKLETVQDEESRGNPE 340
R K EK A FE++Q +QDE R + E
Sbjct: 160 RLKREKDALFEEEQTLQNKIQDELQRRSYE 189
>SPAC3C7.02c |||protein kinase inhibitor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 383
Score = 24.2 bits (50), Expect = 9.1
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +2
Query: 284 KKQKKLETVQDEESRGNPEPKITILE 361
KK+K L ++ D SR PK+ +E
Sbjct: 144 KKEKLLNSIYDVRSRDPESPKLITME 169
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,319,870
Number of Sequences: 5004
Number of extensions: 19905
Number of successful extensions: 70
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 68
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 162176800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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