BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20235 (445 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g15810.1 68418.m01850 N2,N2-dimethylguanosine tRNA methyltran... 41 4e-04 At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran... 40 0.001 At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein... 29 1.9 At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein... 29 1.9 At2g22100.1 68415.m02625 RNA recognition motif (RRM)-containing ... 28 2.5 At1g52430.1 68414.m05919 ubiquitin carboxyl-terminal hydrolase-r... 27 4.3 At1g52450.1 68414.m05921 ubiquitin carboxyl-terminal hydrolase-r... 27 7.5 At5g27140.1 68418.m03239 SAR DNA-binding protein, putative stron... 26 10.0 At1g03560.1 68414.m00337 pentatricopeptide (PPR) repeat-containi... 26 10.0 >At5g15810.1 68418.m01850 N2,N2-dimethylguanosine tRNA methyltransferase family protein similar to SP|Q9P804 N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC 2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase) {Schizosaccharomyces pombe}; contains Pfam profile PF02005: N2,N2-dimethylguanosine tRNA methyltransferase Length = 691 Score = 40.7 bits (91), Expect = 4e-04 Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 2/47 (4%) Frame = +3 Query: 114 MET-ISNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFI 248 MET +++ IKEG+A++ + + +VF+N Q NRD+SIAVL FI Sbjct: 99 METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFI 145 Score = 33.9 bits (74), Expect = 0.050 Identities = 17/41 (41%), Positives = 27/41 (65%) Frame = +2 Query: 317 EESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIAND 439 E +R +P +LEALSA+GL ++RYA+E+ ++A D Sbjct: 211 ESARRELKPP-RVLEALSASGLRALRYAREVEGIGQVVALD 250 >At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltransferase family protein similar to N2,N2-dimethylguanosine tRNA methyltransferase [Homo sapiens] GI:11066198; contains Pfam profile PF02005: N2,N2-dimethylguanosine tRNA methyltransferase Length = 599 Score = 39.5 bits (88), Expect = 0.001 Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 2/47 (4%) Frame = +3 Query: 114 MET-ISNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFI 248 MET +++ IKEG+A+I + + +VF+N Q NRD+SIAVL F+ Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFL 47 Score = 33.1 bits (72), Expect = 0.087 Identities = 17/35 (48%), Positives = 25/35 (71%), Gaps = 2/35 (5%) Frame = +2 Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIA--NDLS 445 I I +ALSA+GL ++RYA+EI ++A NDL+ Sbjct: 132 ILIFQALSASGLRALRYAREIEGIGQVVALDNDLA 166 >At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein (ZWICHEL) identical to kinesin-like protein GI:2224925 from [Arabidopsis thaliana] Length = 1260 Score = 28.7 bits (61), Expect = 1.9 Identities = 14/46 (30%), Positives = 26/46 (56%) Frame = +2 Query: 236 YAVY*RYKAEKLARFEKKQKKLETVQDEESRGNPEPKITILEALSA 373 + VY + + +E+ QKK+E + DE+ N + ++T+ E L A Sbjct: 617 FEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKN-QQEVTLREELEA 661 >At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein (ZWICHEL) identical to kinesin-like protein GI:2224925 from [Arabidopsis thaliana] Length = 1259 Score = 28.7 bits (61), Expect = 1.9 Identities = 14/46 (30%), Positives = 26/46 (56%) Frame = +2 Query: 236 YAVY*RYKAEKLARFEKKQKKLETVQDEESRGNPEPKITILEALSA 373 + VY + + +E+ QKK+E + DE+ N + ++T+ E L A Sbjct: 616 FEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKN-QQEVTLREELEA 660 >At2g22100.1 68415.m02625 RNA recognition motif (RRM)-containing protein similar to UBP1 interacting protein 1a [Arabidopsis thaliana] GI:19574236; contains Pfam profile: PF00076 RNA recognition motif (aka RRM, RBD, or RNP domain) Length = 382 Score = 28.3 bits (60), Expect = 2.5 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +2 Query: 263 EKLARFEKKQKKLETVQDEESRGNPEP 343 EK + +KK KK E V++EE +P P Sbjct: 48 EKPEKSKKKSKKYEEVEEEEKSPSPSP 74 >At1g52430.1 68414.m05919 ubiquitin carboxyl-terminal hydrolase-related contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein of unknown function (DUF629), PF04781: Protein of unknown function (DUF627) Length = 1136 Score = 27.5 bits (58), Expect = 4.3 Identities = 9/23 (39%), Positives = 18/23 (78%) Frame = +3 Query: 168 LTTEKVFYNPVQEFNRDLSIAVL 236 ++ +K+F P++E+N D+ +AVL Sbjct: 496 ISVDKIFPKPIREYNLDIWLAVL 518 >At1g52450.1 68414.m05921 ubiquitin carboxyl-terminal hydrolase-related contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein of unknown function (DUF629), PF04781: Protein of unknown function (DUF627) Length = 1136 Score = 26.6 bits (56), Expect = 7.5 Identities = 9/22 (40%), Positives = 17/22 (77%) Frame = +3 Query: 171 TTEKVFYNPVQEFNRDLSIAVL 236 + +K+F P++E+N D+ +AVL Sbjct: 497 SVDKIFPKPIREYNLDIWLAVL 518 >At5g27140.1 68418.m03239 SAR DNA-binding protein, putative strong similarity to SAR DNA-binding protein-1 [Pisum sativum] GI:3132696; contains Pfam profile PF01798: Putative snoRNA binding domain Length = 445 Score = 26.2 bits (55), Expect = 10.0 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +2 Query: 257 KAEKLARFEKKQKKLETVQDEESRGNPEPK 346 K E+ ++ KK+ +LETV+D + E K Sbjct: 396 KTEECSKKRKKEAELETVEDPAKKSKQEVK 425 >At1g03560.1 68414.m00337 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 660 Score = 26.2 bits (55), Expect = 10.0 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = +2 Query: 290 QKKLETVQDEESRGNPEPKITILEALSA 373 QK +E ++D E+RG+ KIT + + A Sbjct: 274 QKAMEKLRDMETRGHEADKITYMTMIQA 301 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,897,718 Number of Sequences: 28952 Number of extensions: 105144 Number of successful extensions: 327 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 321 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 327 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 712739520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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