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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20235
         (445 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g15810.1 68418.m01850 N2,N2-dimethylguanosine tRNA methyltran...    41   4e-04
At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran...    40   0.001
At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein...    29   1.9  
At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein...    29   1.9  
At2g22100.1 68415.m02625 RNA recognition motif (RRM)-containing ...    28   2.5  
At1g52430.1 68414.m05919 ubiquitin carboxyl-terminal hydrolase-r...    27   4.3  
At1g52450.1 68414.m05921 ubiquitin carboxyl-terminal hydrolase-r...    27   7.5  
At5g27140.1 68418.m03239 SAR DNA-binding protein, putative stron...    26   10.0 
At1g03560.1 68414.m00337 pentatricopeptide (PPR) repeat-containi...    26   10.0 

>At5g15810.1 68418.m01850 N2,N2-dimethylguanosine tRNA
           methyltransferase family protein similar to SP|Q9P804
           N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC
           2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           {Schizosaccharomyces pombe}; contains Pfam profile
           PF02005: N2,N2-dimethylguanosine tRNA methyltransferase
          Length = 691

 Score = 40.7 bits (91), Expect = 4e-04
 Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 2/47 (4%)
 Frame = +3

Query: 114 MET-ISNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFI 248
           MET +++   IKEG+A++ +  + +VF+N  Q  NRD+SIAVL  FI
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFI 145



 Score = 33.9 bits (74), Expect = 0.050
 Identities = 17/41 (41%), Positives = 27/41 (65%)
 Frame = +2

Query: 317 EESRGNPEPKITILEALSATGLXSIRYAKEIPYATNIIAND 439
           E +R   +P   +LEALSA+GL ++RYA+E+     ++A D
Sbjct: 211 ESARRELKPP-RVLEALSASGLRALRYAREVEGIGQVVALD 250


>At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA
           methyltransferase family protein similar to
           N2,N2-dimethylguanosine tRNA methyltransferase [Homo
           sapiens] GI:11066198; contains Pfam profile PF02005:
           N2,N2-dimethylguanosine tRNA methyltransferase
          Length = 599

 Score = 39.5 bits (88), Expect = 0.001
 Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 2/47 (4%)
 Frame = +3

Query: 114 MET-ISNLKTIKEGQAQICLTTE-KVFYNPVQEFNRDLSIAVLTLFI 248
           MET +++   IKEG+A+I +  + +VF+N  Q  NRD+SIAVL  F+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFL 47



 Score = 33.1 bits (72), Expect = 0.087
 Identities = 17/35 (48%), Positives = 25/35 (71%), Gaps = 2/35 (5%)
 Frame = +2

Query: 347 ITILEALSATGLXSIRYAKEIPYATNIIA--NDLS 445
           I I +ALSA+GL ++RYA+EI     ++A  NDL+
Sbjct: 132 ILIFQALSASGLRALRYAREIEGIGQVVALDNDLA 166


>At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein
           (ZWICHEL) identical to kinesin-like protein GI:2224925
           from [Arabidopsis thaliana]
          Length = 1260

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 14/46 (30%), Positives = 26/46 (56%)
 Frame = +2

Query: 236 YAVY*RYKAEKLARFEKKQKKLETVQDEESRGNPEPKITILEALSA 373
           + VY +   +    +E+ QKK+E + DE+   N + ++T+ E L A
Sbjct: 617 FEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKN-QQEVTLREELEA 661


>At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein
           (ZWICHEL) identical to kinesin-like protein GI:2224925
           from [Arabidopsis thaliana]
          Length = 1259

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 14/46 (30%), Positives = 26/46 (56%)
 Frame = +2

Query: 236 YAVY*RYKAEKLARFEKKQKKLETVQDEESRGNPEPKITILEALSA 373
           + VY +   +    +E+ QKK+E + DE+   N + ++T+ E L A
Sbjct: 616 FEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKN-QQEVTLREELEA 660


>At2g22100.1 68415.m02625 RNA recognition motif (RRM)-containing
           protein similar to UBP1 interacting protein 1a
           [Arabidopsis thaliana] GI:19574236; contains Pfam
           profile: PF00076 RNA recognition motif (aka RRM, RBD, or
           RNP domain)
          Length = 382

 Score = 28.3 bits (60), Expect = 2.5
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +2

Query: 263 EKLARFEKKQKKLETVQDEESRGNPEP 343
           EK  + +KK KK E V++EE   +P P
Sbjct: 48  EKPEKSKKKSKKYEEVEEEEKSPSPSP 74


>At1g52430.1 68414.m05919 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1136

 Score = 27.5 bits (58), Expect = 4.3
 Identities = 9/23 (39%), Positives = 18/23 (78%)
 Frame = +3

Query: 168 LTTEKVFYNPVQEFNRDLSIAVL 236
           ++ +K+F  P++E+N D+ +AVL
Sbjct: 496 ISVDKIFPKPIREYNLDIWLAVL 518


>At1g52450.1 68414.m05921 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1136

 Score = 26.6 bits (56), Expect = 7.5
 Identities = 9/22 (40%), Positives = 17/22 (77%)
 Frame = +3

Query: 171 TTEKVFYNPVQEFNRDLSIAVL 236
           + +K+F  P++E+N D+ +AVL
Sbjct: 497 SVDKIFPKPIREYNLDIWLAVL 518


>At5g27140.1 68418.m03239 SAR DNA-binding protein, putative strong
           similarity to SAR DNA-binding protein-1 [Pisum sativum]
           GI:3132696; contains Pfam profile PF01798: Putative
           snoRNA binding domain
          Length = 445

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +2

Query: 257 KAEKLARFEKKQKKLETVQDEESRGNPEPK 346
           K E+ ++  KK+ +LETV+D   +   E K
Sbjct: 396 KTEECSKKRKKEAELETVEDPAKKSKQEVK 425


>At1g03560.1 68414.m00337 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 660

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = +2

Query: 290 QKKLETVQDEESRGNPEPKITILEALSA 373
           QK +E ++D E+RG+   KIT +  + A
Sbjct: 274 QKAMEKLRDMETRGHEADKITYMTMIQA 301


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,897,718
Number of Sequences: 28952
Number of extensions: 105144
Number of successful extensions: 327
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 321
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 327
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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