Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20233
(494 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC725.09c |hob3||BAR adaptor protein Hob3|Schizosaccharomyces ... 34 0.010
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.22
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 28 0.67
SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch... 28 0.67
SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|ch... 27 1.2
SPAC343.09 |ubx3|mug39|UBX domain protein Ubx3|Schizosaccharomyc... 27 1.2
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 26 2.7
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 26 3.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 3.6
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 26 3.6
SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon... 26 3.6
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 4.7
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 25 4.7
SPAC27F1.06c |||FKBP-type peptidyl-prolyl cis-trans isomerase |S... 25 6.3
SPAC3G6.10c |||GARP complex subunit Vps51 |Schizosaccharomyces p... 25 8.3
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 25 8.3
>SPBC725.09c |hob3||BAR adaptor protein Hob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 264
Score = 34.3 bits (75), Expect = 0.010
Identities = 20/71 (28%), Positives = 34/71 (47%)
Frame = +3
Query: 12 DDVKVTKGREQLEEAKRTYEILNSELHDELPALYDSRVLFYVNNLQTLFSAEQVFHFESS 191
D K+ + ++ AK YE LN++L ELP L RV + + + L + F E
Sbjct: 159 DTTKLPRTEKEAAMAKEVYETLNNQLVSELPQLIALRVPYLDPSFEALVKIQLRFCREGY 218
Query: 192 KVFAELEAVTD 224
+ A+++ D
Sbjct: 219 EKMAQVQQYFD 229
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.9 bits (64), Expect = 0.22
Identities = 17/46 (36%), Positives = 22/46 (47%)
Frame = +2
Query: 356 PPSPALNGDSPTLSGNKEPAPVKETAPDPKDPGTPVKEPAPVAKEP 493
PPSPA +P + +K P P+ + P P P PVK P P
Sbjct: 151 PPSPA---SAPPIP-SKAP-PIPSSLPPPAQPAAPVKSPPSAPSLP 191
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 28.3 bits (60), Expect = 0.67
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +2
Query: 356 PPSPALNGDSPTLSGNKEPAPVKETAPDPKDPGTPVKEPAPVA 484
PP PT SG + P PV++ P +P TPV +PVA
Sbjct: 928 PPKVGETYHPPTASGTRVP-PVQQ--PSHPNPYTPVAPQSPVA 967
>SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 28.3 bits (60), Expect = 0.67
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -1
Query: 197 HLGAFEMKDLLGREERLQVVDVEQYP 120
HL AFE K + G EE LQ +D+++ P
Sbjct: 426 HLRAFERKGMAGLEEELQ-LDIDRIP 450
>SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 870
Score = 27.5 bits (58), Expect = 1.2
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 3/47 (6%)
Frame = +2
Query: 350 QTPPSPALNGDSPTLSGNKEPAPVKE---TAPDPKDPGTPVKEPAPV 481
+T SP ++ D+P LSGN +P P+ T P +D E P+
Sbjct: 209 ETATSPEIS-DAPPLSGNVDPLPINSPPLTNPVARDIDQTEPEDGPL 254
>SPAC343.09 |ubx3|mug39|UBX domain protein Ubx3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 410
Score = 27.5 bits (58), Expect = 1.2
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 9 RDDVKVTKGREQLEEAKRTYEILNSELHDELP 104
R+++ V++GR LE YE+ + LH+ +P
Sbjct: 13 RNNIDVSQGRFFLESTNWNYELATALLHEVIP 44
>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2386
Score = 26.2 bits (55), Expect = 2.7
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +3
Query: 24 VTKGREQLEEAKRTYEILNSELHDELPALYDSRVLFYV 137
V K E+ RTYE+ E+ + L+D R LF +
Sbjct: 492 VAKVNEKTNSIFRTYEVHGCEVCNSFCLLFDERSLFKI 529
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 25.8 bits (54), Expect = 3.6
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +2
Query: 365 PALNGDSPTLSGNKEPAPVKETAPDPKDPGTPVKEPAPVAKEP 493
P LN P +EP E P+ PG P PV +EP
Sbjct: 92 PLLNELVPEEPLPREPPLPNEPVPEEPLPGEPPLPDEPVPEEP 134
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 3.6
Identities = 12/41 (29%), Positives = 15/41 (36%)
Frame = +2
Query: 353 TPPSPALNGDSPTLSGNKEPAPVKETAPDPKDPGTPVKEPA 475
TPP + P +S P P P P P P P+
Sbjct: 1689 TPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPS 1729
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 25.8 bits (54), Expect = 3.6
Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 3/54 (5%)
Frame = +3
Query: 21 KVTKGREQLEEAKRTYEILNSEL---HDELPALYDSRVLFYVNNLQTLFSAEQV 173
K+++GR LE A + EIL +L +L +L +S F +N + L+S Q+
Sbjct: 565 KLSEGRAHLETANKENEILKQQLELSESKLASLLNSYQSF-INKKEHLYSFLQL 617
>SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component
of oxoglutarate dehydrogenase complex
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.8 bits (54), Expect = 3.6
Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
Frame = +2
Query: 404 KEPAPVK-ETAPDPKDPGTPVKEPAPVAKEP 493
KE AP + APD K+P +P P EP
Sbjct: 169 KESAPSSTKPAPDAKEPEFSSPKPKPAKSEP 199
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.4 bits (53), Expect = 4.7
Identities = 10/28 (35%), Positives = 12/28 (42%)
Frame = +2
Query: 410 PAPVKETAPDPKDPGTPVKEPAPVAKEP 493
PAP+ +P P P KE A P
Sbjct: 893 PAPIPAPVAEPAPPAAPAKEVVEKAPSP 920
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.4 bits (53), Expect = 4.7
Identities = 12/44 (27%), Positives = 21/44 (47%), Gaps = 2/44 (4%)
Frame = +2
Query: 350 QTPPSPALNGDSPTLSGNKE--PAPVKETAPDPKDPGTPVKEPA 475
+ PP PA + ++P++S + P+P +P P PA
Sbjct: 1235 KAPPVPAPSSEAPSVSTPRSSVPSPHSNASPSPTSSSMASAAPA 1278
>SPAC27F1.06c |||FKBP-type peptidyl-prolyl cis-trans isomerase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 362
Score = 25.0 bits (52), Expect = 6.3
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 12 DDVKVTKGREQLEEAKRTYEILNSELHD 95
DD+ VT E+ EE E LNS+ D
Sbjct: 154 DDISVTSSEEEEEENGARIEELNSDEED 181
>SPAC3G6.10c |||GARP complex subunit Vps51 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 159
Score = 24.6 bits (51), Expect = 8.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 6/35 (17%)
Frame = +2
Query: 365 PALNGDSPTLSGNKEPA------PVKETAPDPKDP 451
P N S T+S ++P+ PV+ETA D DP
Sbjct: 16 PGSNSSSSTVSTTRKPSSSNTVSPVEETALDKVDP 50
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 24.6 bits (51), Expect = 8.3
Identities = 12/45 (26%), Positives = 20/45 (44%)
Frame = +2
Query: 356 PPSPALNGDSPTLSGNKEPAPVKETAPDPKDPGTPVKEPAPVAKE 490
PP P + + G++ AP + P+PK T + E + E
Sbjct: 775 PPPPPPPPPAVSAGGSRYYAPAPQAEPEPKIDETSLTEEQKIQLE 819
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,486,109
Number of Sequences: 5004
Number of extensions: 23610
Number of successful extensions: 129
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 194131776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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