Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20230
(640 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 182 3e-48
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 182 3e-48
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 178 3e-47
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 175 3e-46
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.002
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.1
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.5
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.5
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.3
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 182 bits (443), Expect = 3e-48
Identities = 88/96 (91%), Positives = 89/96 (92%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG
Sbjct: 15 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 74
Query: 435 QTREHALLAFTLGVKQLIGRSKQNGSTEPPYSEPRF 542
QTREHALLAFTLGVKQLI + STEPPYSE RF
Sbjct: 75 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRF 110
Score = 44.4 bits (100), Expect = 9e-07
Identities = 25/47 (53%), Positives = 25/47 (53%)
Frame = +1
Query: 493 GVNKMVPLNHHTVSPDFEEIKKEVSSYIKKIGLQPTCCRFRAHFLGW 633
GVNKM FEEIKKEVSSYIKKIG P F GW
Sbjct: 94 GVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF-VPISGW 139
Score = 33.5 bits (73), Expect = 0.002
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = +1
Query: 214 WVLDKLKAERERGI 255
WVLDKLKAERERGI
Sbjct: 1 WVLDKLKAERERGI 14
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 182 bits (443), Expect = 3e-48
Identities = 88/96 (91%), Positives = 89/96 (92%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG
Sbjct: 72 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 131
Query: 435 QTREHALLAFTLGVKQLIGRSKQNGSTEPPYSEPRF 542
QTREHALLAFTLGVKQLI + STEPPYSE RF
Sbjct: 132 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRF 167
Score = 151 bits (367), Expect = 4e-39
Identities = 70/71 (98%), Positives = 70/71 (98%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 223 DKLKAERERGI 255
DKLKAERERGI
Sbjct: 61 DKLKAERERGI 71
Score = 44.4 bits (100), Expect = 9e-07
Identities = 25/47 (53%), Positives = 25/47 (53%)
Frame = +1
Query: 493 GVNKMVPLNHHTVSPDFEEIKKEVSSYIKKIGLQPTCCRFRAHFLGW 633
GVNKM FEEIKKEVSSYIKKIG P F GW
Sbjct: 151 GVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF-VPISGW 196
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 178 bits (434), Expect = 3e-47
Identities = 86/94 (91%), Positives = 87/94 (92%)
Frame = +3
Query: 261 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 440
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 441 REHALLAFTLGVKQLIGRSKQNGSTEPPYSEPRF 542
REHALLAFTLGVKQLI + STEPPYSE RF
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRF 94
Score = 44.4 bits (100), Expect = 9e-07
Identities = 25/47 (53%), Positives = 25/47 (53%)
Frame = +1
Query: 493 GVNKMVPLNHHTVSPDFEEIKKEVSSYIKKIGLQPTCCRFRAHFLGW 633
GVNKM FEEIKKEVSSYIKKIG P F GW
Sbjct: 78 GVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF-VPISGW 123
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 175 bits (426), Expect = 3e-46
Identities = 84/96 (87%), Positives = 87/96 (90%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNG
Sbjct: 72 TIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNG 131
Query: 435 QTREHALLAFTLGVKQLIGRSKQNGSTEPPYSEPRF 542
QTREHALLAFTLGVKQLI + T+PPYSE RF
Sbjct: 132 QTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARF 167
Score = 151 bits (367), Expect = 4e-39
Identities = 70/71 (98%), Positives = 70/71 (98%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 223 DKLKAERERGI 255
DKLKAERERGI
Sbjct: 61 DKLKAERERGI 71
Score = 40.7 bits (91), Expect = 1e-05
Identities = 24/47 (51%), Positives = 25/47 (53%)
Frame = +1
Query: 493 GVNKMVPLNHHTVSPDFEEIKKEVSSYIKKIGLQPTCCRFRAHFLGW 633
GVNKM + FEEIKKEVSSYIKKIG F GW
Sbjct: 151 GVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAF-VPISGW 196
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.002
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 300 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.47
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 55 KTHINIVVIGHVDSGKST 108
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.1
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 64 INIVVIGHVDSGKST 108
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 2.5
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 56 FSLPIFG*SRITNCV*Y 6
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 386
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.3
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 386
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 187,786
Number of Sequences: 438
Number of extensions: 4023
Number of successful extensions: 26
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19193721
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -