BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20218 (585 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 29 0.66 SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 28 1.2 SPBC19F5.02c |||U3 snoRNP protein Utp4 |Schizosaccharomyces pomb... 27 2.7 SPAC17G8.07 |||YEATS family protein|Schizosaccharomyces pombe|ch... 25 6.2 SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 6.2 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 6.2 SPAP7G5.06 |||amino acid permease, unknown 4|Schizosaccharomyces... 25 8.1 >SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 28.7 bits (61), Expect = 0.66 Identities = 16/72 (22%), Positives = 36/72 (50%), Gaps = 1/72 (1%) Frame = -2 Query: 509 ASLRVEVGRVLGAGRDDAVIDLLGGAARARYGQTVIGPVGRTVYTAALQV-CLYDINSLL 333 ++LR ++ + R +A+ L G G + G+ ++ +A+ + L+D+ L+ Sbjct: 427 STLRSDIEKSSKFSRTNALKTLYGSKPHVFSGSSQRRATGKNIHESAIALFSLHDVPGLM 486 Query: 332 DVLVYRILSNGS 297 + L+Y+ GS Sbjct: 487 EELIYKSRGRGS 498 >SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1811 Score = 27.9 bits (59), Expect = 1.2 Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 1/50 (2%) Frame = +3 Query: 60 HELHR*ELLQVRSDRSHHCRHYCFHCRVLRLLWRCQREPLH-DNNVFSIP 206 HE+ R V + HH + C + L+ C E H DN + +IP Sbjct: 1594 HEISRFNSQSVFKSKKHHLKSIVVKCTLQLLMLNCLWELFHSDNMLTNIP 1643 >SPBC19F5.02c |||U3 snoRNP protein Utp4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 710 Score = 26.6 bits (56), Expect = 2.7 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = +3 Query: 267 LEDSVMRNLNASITQYPVDKN 329 +EDS + N+ AS+ + VDKN Sbjct: 421 VEDSFLSNIGASLLSFTVDKN 441 >SPAC17G8.07 |||YEATS family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 217 Score = 25.4 bits (53), Expect = 6.2 Identities = 8/23 (34%), Positives = 17/23 (73%) Frame = -1 Query: 93 ELVEVLIGEVHVRIRVYFSPDAN 25 E++E GE + +R++F+P+A+ Sbjct: 81 EVIETGWGEFDIMVRIFFAPEAH 103 >SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 703 Score = 25.4 bits (53), Expect = 6.2 Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 4/84 (4%) Frame = -2 Query: 359 CLYDINSLLD-VLVYRILSNGSV--QVAHNRIFQVRM-LM*PAMPTASSATNMIKRNTEN 192 C I+ +L VL+ I +GSV +A N +V L ++ A+S +I +N EN Sbjct: 383 CTSFISGILQLVLLNNIREDGSVISALAQNSFHKVESALANVSVAWANSTNQIILKNQEN 442 Query: 191 VIIMQWFSLTAPQQPKNATMKTIM 120 + + S+ N+T+ T M Sbjct: 443 INNNMFGSIHNTTLSLNSTLNTFM 466 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.4 bits (53), Expect = 6.2 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = -2 Query: 161 APQQPKNATMKTIMPTMMRT 102 AP QPK + +I+PT+ RT Sbjct: 810 APVQPKTSQASSILPTVPRT 829 >SPAP7G5.06 |||amino acid permease, unknown 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 583 Score = 25.0 bits (52), Expect = 8.1 Identities = 17/70 (24%), Positives = 31/70 (44%) Frame = -3 Query: 523 NQFSKHPFEWKLVEFSVQAATTPSLISWAEQHVLGMGRP*SAQSAGLFIPQHCKSVCMIS 344 N F HP E +++ V+ A TPS ++ ++ + + G+ + +H K M Sbjct: 32 NVFDIHPTESEVIPGEVEYADTPSHQNFLQKFFSDFKPVKADREDGVALKRHLKGRHMQM 91 Query: 343 IVFWTFLSTG 314 I + TG Sbjct: 92 IAIGGAIGTG 101 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,186,704 Number of Sequences: 5004 Number of extensions: 42529 Number of successful extensions: 161 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 252150250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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