Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20189
         (474 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P09335 Cluster: Low molecular 30 kDa lipoprotein PBMHP-...   142   4e-33
UniRef50_Q75RW3 Cluster: BmLSP-T; n=2; Bombyx mori|Rep: BmLSP-T ...    77   2e-13
UniRef50_P19616 Cluster: Microvitellogenin precursor; n=3; Mandu...    77   2e-13
UniRef50_Q00802 Cluster: Low molecular mass 30 kDa lipoprotein 1...    73   3e-12
UniRef50_Q2PQU4 Cluster: Putative paralytic peptide-binding prot...    71   1e-11
UniRef50_P09334 Cluster: Low molecular 30 kDa lipoprotein PBMHP-...    70   2e-11
UniRef50_Q76IB6 Cluster: Growth blocking peptide binding protein...    57   2e-07
UniRef50_UPI0000D55E7A Cluster: PREDICTED: hypothetical protein;...    31   9.8  
UniRef50_Q17PV0 Cluster: Leucine-rich transmembrane protein; n=1...    31   9.8  
UniRef50_A2FX22 Cluster: Putative uncharacterized protein; n=1; ...    31   9.8  

>UniRef50_P09335 Cluster: Low molecular 30 kDa lipoprotein PBMHP-12
           precursor; n=5; Bombyx mori|Rep: Low molecular 30 kDa
           lipoprotein PBMHP-12 precursor - Bombyx mori (Silk moth)
          Length = 264

 Score =  142 bits (344), Expect = 4e-33
 Identities = 63/78 (80%), Positives = 71/78 (91%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLWVGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKLGP 404
           VNNLIID  RNTMEYCYKLWVGNGQ IV+KYFP +FRLIMAGN+VKLIYRNYNLALKLG 
Sbjct: 69  VNNLIIDKRRNTMEYCYKLWVGNGQDIVKKYFPLSFRLIMAGNYVKLIYRNYNLALKLGS 128

Query: 405 TLDPANERLAYGDGKEKN 458
           T +P+NER+AYGDG +K+
Sbjct: 129 TTNPSNERIAYGDGVDKH 146



 Score = 70.1 bits (164), Expect = 2e-11
 Identities = 37/68 (54%), Positives = 41/68 (60%)
 Frame = +2

Query: 20  MKFLVVFASCVLXXXXXXXXXXXXXXXXXXXXXXXXLYNSILTGDYDSAVRQSLEYENQG 199
           MK LVVFA CV                         LYNSILTGDYDSAVR+SLEYE+QG
Sbjct: 1   MKLLVVFAMCVPAASAGVVELSADSMSPSNQDLEDKLYNSILTGDYDSAVRKSLEYESQG 60

Query: 200 KGSIIQNV 223
           +GSI+QNV
Sbjct: 61  QGSIVQNV 68


>UniRef50_Q75RW3 Cluster: BmLSP-T; n=2; Bombyx mori|Rep: BmLSP-T -
           Bombyx mori (Silk moth)
          Length = 267

 Score = 77.4 bits (182), Expect = 2e-13
 Identities = 38/79 (48%), Positives = 50/79 (63%), Gaps = 2/79 (2%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLW--VGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKL 398
           VN LI +  RN  +  YKLW  +   Q IV++YFP  FR I + N VK+I +  NLA+KL
Sbjct: 70  VNRLIRENKRNICDLAYKLWDYMDESQEIVKEYFPVIFRQIFSENSVKIINKRDNLAIKL 129

Query: 399 GPTLDPANERLAYGDGKEK 455
           G  LD  N+R+AYGD  +K
Sbjct: 130 GDALDSDNDRVAYGDANDK 148


>UniRef50_P19616 Cluster: Microvitellogenin precursor; n=3; Manduca
           sexta|Rep: Microvitellogenin precursor - Manduca sexta
           (Tobacco hawkmoth) (Tobacco hornworm)
          Length = 249

 Score = 77.0 bits (181), Expect = 2e-13
 Identities = 35/77 (45%), Positives = 50/77 (64%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLWVGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKLGP 404
           VN LI D  RNTMEY Y+LW    + IV++ FP  FR+++  + +KLI +  NLA+KLG 
Sbjct: 56  VNRLIRDSQRNTMEYAYQLWSLEARDIVKERFPIQFRMMLGEHSIKLINKRDNLAMKLGV 115

Query: 405 TLDPANERLAYGDGKEK 455
             D + +R+AYG   +K
Sbjct: 116 ATDNSGDRIAYGAADDK 132



 Score = 37.1 bits (82), Expect = 0.20
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +2

Query: 128 LYNSILTGDYDSAVRQSLEYENQGKGSII 214
           +YN+++ GD D AV +S E + QGKG II
Sbjct: 24  IYNNVVIGDIDGAVAKSKELQKQGKGDII 52


>UniRef50_Q00802 Cluster: Low molecular mass 30 kDa lipoprotein 19G1
           precursor; n=3; Bombyx mori|Rep: Low molecular mass 30
           kDa lipoprotein 19G1 precursor - Bombyx mori (Silk moth)
          Length = 256

 Score = 72.9 bits (171), Expect = 3e-12
 Identities = 36/77 (46%), Positives = 46/77 (59%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLWVGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKLGP 404
           VN LI +   N MEY Y+LW+   + IVR  FP  FRLI A N +KL+Y+   LAL L  
Sbjct: 63  VNKLIRNNKMNCMEYAYQLWLQGSKDIVRDCFPVEFRLIFAENAIKLMYKRDGLALTLSN 122

Query: 405 TLDPANERLAYGDGKEK 455
            +   + R  YGDGK+K
Sbjct: 123 DVQGDDGRPRYGDGKDK 139



 Score = 35.1 bits (77), Expect = 0.80
 Identities = 15/32 (46%), Positives = 20/32 (62%)
 Frame = +2

Query: 128 LYNSILTGDYDSAVRQSLEYENQGKGSIIQNV 223
           LYNS++  DYDSAV +S     + K  +I NV
Sbjct: 31  LYNSVVVADYDSAVEKSKHLYEEKKSEVITNV 62


>UniRef50_Q2PQU4 Cluster: Putative paralytic peptide-binding
           protein; n=1; Bombyx mori|Rep: Putative paralytic
           peptide-binding protein - Bombyx mori (Silk moth)
          Length = 436

 Score = 70.9 bits (166), Expect = 1e-11
 Identities = 33/76 (43%), Positives = 46/76 (60%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLWVGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKLGP 404
           V+ L+  G +N M + YKLW    + IV  YFP  F+LI+    +KLI  +YN ALKL  
Sbjct: 242 VSRLVSQGIKNAMSFAYKLWHEGHKDIVEDYFPSEFQLILDQKRIKLIGNHYNQALKLDA 301

Query: 405 TLDPANERLAYGDGKE 452
            +D   +RL +GDGK+
Sbjct: 302 NVDRYKDRLTWGDGKD 317


>UniRef50_P09334 Cluster: Low molecular 30 kDa lipoprotein PBMHP-6
           precursor; n=2; Bombyx mori|Rep: Low molecular 30 kDa
           lipoprotein PBMHP-6 precursor - Bombyx mori (Silk moth)
          Length = 256

 Score = 70.1 bits (164), Expect = 2e-11
 Identities = 35/81 (43%), Positives = 51/81 (62%), Gaps = 1/81 (1%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLWVGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKLGP 404
           V  LI +G RNTM++ Y+LW  +G+ IV+ YFP  FR+I     VKLI +  + ALKL  
Sbjct: 65  VKRLIENGKRNTMDFAYQLWTKDGKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALKL-- 122

Query: 405 TLDPAN-ERLAYGDGKEKNKR 464
            +D  N  ++A+GD K+K  +
Sbjct: 123 -IDQQNHNKIAFGDSKDKTSK 142


>UniRef50_Q76IB6 Cluster: Growth blocking peptide binding protein;
           n=1; Mythimna separata|Rep: Growth blocking peptide
           binding protein - Pseudaletia separata (Oriental
           armyworm) (Mythimna separata)
          Length = 430

 Score = 57.2 bits (132), Expect = 2e-07
 Identities = 28/73 (38%), Positives = 39/73 (53%)
 Frame = +3

Query: 225 VNNLIIDGSRNTMEYCYKLWVGNGQHIVRKYFPYNFRLIMAGNFVKLIYRNYNLALKLGP 404
           V  L+    R  M + YKLW G  + IVR +FP  F+ I   + V ++ + Y   LKL  
Sbjct: 233 VTRLMTAFPRKLMSFAYKLWHGGAKEIVRNHFPKAFQHIFNEDAVTIVNKQYQQPLKLDV 292

Query: 405 TLDPANERLAYGD 443
             D  N+RLA+GD
Sbjct: 293 NTDSMNDRLAWGD 305


>UniRef50_UPI0000D55E7A Cluster: PREDICTED: hypothetical protein;
           n=1; Tribolium castaneum|Rep: PREDICTED: hypothetical
           protein - Tribolium castaneum
          Length = 1132

 Score = 31.5 bits (68), Expect = 9.8
 Identities = 18/42 (42%), Positives = 24/42 (57%)
 Frame = +1

Query: 214 PECKLTT*SLTEVGTPWSTATSCGSATDSTLSESTSPITLDS 339
           PE  +TT + TEV TP  T T   ++ DS     TS +TL+S
Sbjct: 97  PETVITTQTSTEVTTPVQTTTPVPNSADSNAKTQTS-VTLNS 137


>UniRef50_Q17PV0 Cluster: Leucine-rich transmembrane protein; n=1;
           Aedes aegypti|Rep: Leucine-rich transmembrane protein -
           Aedes aegypti (Yellowfever mosquito)
          Length = 999

 Score = 31.5 bits (68), Expect = 9.8
 Identities = 14/44 (31%), Positives = 28/44 (63%)
 Frame = +3

Query: 318 FPYNFRLIMAGNFVKLIYRNYNLALKLGPTLDPANERLAYGDGK 449
           FPY   L +  NF++L++R+  ++LK   +LD ++ R+ + D +
Sbjct: 122 FPYLKTLSLYNNFIELVHRDSFVSLKELQSLDLSHNRIVFVDAE 165


>UniRef50_A2FX22 Cluster: Putative uncharacterized protein; n=1;
           Trichomonas vaginalis G3|Rep: Putative uncharacterized
           protein - Trichomonas vaginalis G3
          Length = 628

 Score = 31.5 bits (68), Expect = 9.8
 Identities = 21/62 (33%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
 Frame = +3

Query: 204 APSSRM*VNNLIIDGSRNTMEYCYKLWVGNGQHIV----RKYFPYNFRLIM-AGNFVKLI 368
           AP SR+ VN+ ++D      +   +L   N   +V      YFPYNF + M    FV L+
Sbjct: 6   APPSRLDVNDNLVDLDCQFQQMIEELSENNYNQLVDLVSTNYFPYNFAMAMFTSEFVTLV 65

Query: 369 YR 374
            R
Sbjct: 66  ER 67


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 431,661,517
Number of Sequences: 1657284
Number of extensions: 7940467
Number of successful extensions: 23425
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 22404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23410
length of database: 575,637,011
effective HSP length: 94
effective length of database: 419,852,315
effective search space used: 26450695845
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)