BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20188 (430 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 93 2e-20 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 58 6e-10 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 8e-10 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 55 4e-09 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.6 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 92.7 bits (220), Expect = 2e-20 Identities = 44/98 (44%), Positives = 56/98 (57%) Frame = +2 Query: 92 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEALLRRRR 271 MREIVH+QAGQCGNQ+GA FW I++EHG+D G+Y GTS+ Q ER++VY+NEA Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEA------ 54 Query: 272 AAGSMXXXXXXXXXXXXXXXXXXXXXXGNSXRPDNXVF 385 A G GN RPDN ++ Sbjct: 55 AGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIY 92 Score = 77.0 bits (181), Expect = 1e-15 Identities = 35/53 (66%), Positives = 41/53 (77%) Frame = +1 Query: 271 SGGKYVPRAILLDLEPGTMDAVRSGAYGQLFXAGQLXLPVSXGAGNNWAKGHY 429 +GGKYVPRA+L+DLEPGTMDAV+SG +G LF + S GAGN WAKGHY Sbjct: 55 AGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQS-GAGNIWAKGHY 106 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 58.0 bits (134), Expect = 6e-10 Identities = 29/56 (51%), Positives = 38/56 (67%), Gaps = 1/56 (1%) Frame = +1 Query: 265 AESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFXAGQLXLPVSXGAGNNWAKGHY 429 +E+G GKYVPR+I +DLEP +D VR+G Y LF QL + A NN+A+GHY Sbjct: 54 SETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQL-ITGKEDASNNYARGHY 108 Score = 44.0 bits (99), Expect = 1e-05 Identities = 18/34 (52%), Positives = 21/34 (61%) Frame = +2 Query: 92 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTG 193 MREI+ + GQ G QIG WE+ EHGI P G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 57.6 bits (133), Expect = 8e-10 Identities = 24/53 (45%), Positives = 36/53 (67%) Frame = +2 Query: 95 REIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEA 253 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R V++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55 Score = 51.6 bits (118), Expect = 5e-08 Identities = 22/50 (44%), Positives = 33/50 (66%), Gaps = 1/50 (2%) Frame = +1 Query: 280 KYVPRAILLDLEPGTMDAVRSGAYGQLFXAGQLXLPVS-XGAGNNWAKGH 426 +Y+PRAIL+DLEP ++ + S YG L+ + + + GAGNNWA G+ Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGY 108 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 55.2 bits (127), Expect = 4e-09 Identities = 27/56 (48%), Positives = 38/56 (67%), Gaps = 1/56 (1%) Frame = +1 Query: 265 AESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFXAGQLXLPVSXGAGNNWAKGHY 429 +E+G GK+VPR+I +DLEP +D VR+G Y LF Q+ + A NN+A+GHY Sbjct: 58 SETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQM-VTGKEDASNNYARGHY 112 Score = 42.3 bits (95), Expect = 3e-05 Identities = 20/53 (37%), Positives = 30/53 (56%) Frame = +2 Query: 92 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNE 250 MRE++ + GQ G QIG WE+ EHGI P G T + ++ + + Y N+ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGF--PTENSEVHKNNSYLND 51 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 26.6 bits (56), Expect = 1.6 Identities = 10/34 (29%), Positives = 21/34 (61%) Frame = +2 Query: 131 NQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERI 232 N++G E+++E+ +DPT + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,266,563 Number of Sequences: 5004 Number of extensions: 17788 Number of successful extensions: 42 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 38 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 154448264 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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