Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20183
         (706 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ...    72   7e-14
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz...    54   2e-08
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz...    49   7e-07
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb...    42   1e-04
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ...    41   2e-04
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom...    28   1.1  
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ...    28   1.5  
SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce...    27   2.0  
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60...    27   3.5  
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    27   3.5  
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ...    26   4.6  
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc...    25   8.0  
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto...    25   8.0  
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom...    25   8.0  

>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1012

 Score = 72.1 bits (169), Expect = 7e-14
 Identities = 45/105 (42%), Positives = 59/105 (56%)
 Frame = +3

Query: 216 VGLRLAEETTCSQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPD 395
           +G    ++ + S VLIIG  GLG EIAKNV L GVKSV L D +  +  DL SQ+    D
Sbjct: 27  LGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTED 86

Query: 396 KIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFLRNLTSYV 530
            IGV RA+ ++ +   LN  V V+     VDEL   +L+N    V
Sbjct: 87  DIGVPRAKVTVSKLAELNQYVPVSV----VDELSTEYLKNFKCVV 127



 Score = 33.5 bits (73), Expect = 0.030
 Identities = 29/128 (22%), Positives = 57/128 (44%), Gaps = 8/128 (6%)
 Frame = +3

Query: 219 GLRLAEETTCSQVLIIGLSGLGAEIAKNVILTGVKS-----VFLLDNEKLKQIDLYSQFL 383
           G    E+       ++G   +G E+ KN  + GV +     + + D + +++ +L  QFL
Sbjct: 418 GSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIEKSNLNRQFL 477

Query: 384 CPPDKIGVNRAEGSLERARGLNPMV--DVTSHTKGV-DELPDSFLRNLTSYVQQVSNKSN 554
             P  +G  ++E +      +NP +   +TS+ + V  E    F       +  V+N  +
Sbjct: 478 FRPRDVGKLKSECASTAVSIMNPSLTGKITSYQERVGPESEGIFGDEFFEKLSLVTNALD 537

Query: 555 SNESTMLV 578
           + E+ M V
Sbjct: 538 NVEARMYV 545


>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
           Rad31|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 307

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 28/84 (33%), Positives = 49/84 (58%)
 Frame = +3

Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
           S+VL+I  S L  EIAKN++L+G+  + +LD+  + + D+  QF      IG  RA    
Sbjct: 32  SRVLLITASPLANEIAKNLVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQLRANVFK 91

Query: 429 ERARGLNPMVDVTSHTKGVDELPD 500
           ++   LNP+V++ + T  + E+ +
Sbjct: 92  KKLHELNPLVEIDTDTSLISEIDE 115



 Score = 33.5 bits (73), Expect = 0.030
 Identities = 14/35 (40%), Positives = 23/35 (65%)
 Frame = +1

Query: 151 VGNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAK 255
           +GN+ +   E     YDRQIRLWG ++Q+ L+ ++
Sbjct: 1   MGNHNINAEEIAL--YDRQIRLWGFNAQQALKQSR 33



 Score = 29.9 bits (64), Expect = 0.37
 Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
 Frame = +2

Query: 506 LTEFDVVCATGLKQEQFERINNACRDSNKKFICGDVWGTVR-ATCSLI 646
           +++F +V AT L  E+F RIN   R  N  F     +G    A C LI
Sbjct: 118 ISKFSMVIATQLDYEEFCRINELTRICNASFYATSCFGLYGFAFCDLI 165


>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
           Fub2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 628

 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 23/81 (28%), Positives = 46/81 (56%)
 Frame = +3

Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
           ++VL++G  G+G E+ KN++++GVK V ++D + +   +L  QFL     +   +A  + 
Sbjct: 26  AKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHVKQPKAIVAA 85

Query: 429 ERARGLNPMVDVTSHTKGVDE 491
           + A   NP V + ++   + E
Sbjct: 86  KTASSFNPNVKLEAYHANIKE 106


>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 401

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 19/75 (25%), Positives = 42/75 (56%)
 Frame = +3

Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
           S VL+IG  GLG    + ++  G+ ++ ++D + + + +L+ Q +    K G+++A  + 
Sbjct: 44  SSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSNLHRQIIHSTSKQGMHKAISAK 103

Query: 429 ERARGLNPMVDVTSH 473
           +    LNP V + ++
Sbjct: 104 QFLEDLNPNVIINTY 118


>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 444

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 23/61 (37%), Positives = 36/61 (59%)
 Frame = +3

Query: 201 PTNPPVGLRLAEETTCSQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQF 380
           P NP   L+ A     S++LIIG  GLG EI K++ L+G + + ++D + +   +L  QF
Sbjct: 32  PENPEETLKSAFS---SKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQF 88

Query: 381 L 383
           L
Sbjct: 89  L 89


>SPBC6B1.05c |||ubiquitin-like conjugating
           enzyme|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 649

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 20/84 (23%), Positives = 40/84 (47%), Gaps = 2/84 (2%)
 Frame = +3

Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQ--FLCPPDKIGVNRAEG 422
           S+ L++G   LG  +A+N++  GV+ V  +D   +   +   Q  F     K  + +AE 
Sbjct: 336 SKCLLLGAGTLGCGVARNLLSWGVRHVTFVDYSTVSYSNPVRQSLFTFEDCKRKLPKAEC 395

Query: 423 SLERARGLNPMVDVTSHTKGVDEL 494
           + +R + + P +  T +   +  L
Sbjct: 396 AAQRLKEIYPNMFSTGYNISIPML 419


>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 500

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +1

Query: 175 SEAEAEQYDRQIRLWGLDSQKRL 243
           + A+ ++YDRQ+RLW  + Q  +
Sbjct: 3   TSAKMQKYDRQVRLWKAEGQNAI 25


>SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1112

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +3

Query: 66  LQEFGTSEVVSKYRAKFIEIINNSIAKNGW 155
           + EFG S VVS+   ++I ++ N  A+ GW
Sbjct: 287 MNEFGPSRVVSEKNREYI-LVKNEGAEGGW 315


>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
           Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 582

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = -1

Query: 628 PYGTPDVAADKLFVAVATSIVDSFELL 548
           P+G+P +  D + VA + S+ D FE L
Sbjct: 74  PFGSPKITKDGVTVARSVSLKDKFENL 100


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 16/34 (47%), Positives = 18/34 (52%)
 Frame = -2

Query: 606 PQINFLLLSRQALLIRSNCSCLRPVAHTTSNSVR 505
           P  NFL   RQ  LI  NC CL   A+T   S+R
Sbjct: 330 PYFNFL---RQKKLIPINCECLNIKAYTLFVSLR 360


>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 485

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 12/72 (16%), Positives = 34/72 (47%)
 Frame = +3

Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
           S V+++G  G+G+ +   +  +GV+ + ++D +++    L    +     +G  +     
Sbjct: 127 SFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSLNRHSIATLQDVGTPKTLAIK 186

Query: 429 ERARGLNPMVDV 464
           +  +   P ++V
Sbjct: 187 KAIKKFAPWIEV 198


>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1679

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 3/56 (5%)
 Frame = -1

Query: 418 SARFTPILSGGHRNWEYRSIC---FSFSLSNKNTLFTPVRITFFAISAPSPDKPMI 260
           SA    IL+GGHRN   +S C   F   L N         + F      +  KP++
Sbjct: 806 SAEAMSILAGGHRNVFVKSTCPIFFQKQLDNYKADHYCFTLDFILTDCVNSPKPLL 861


>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
            Sec74|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 928

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 2/66 (3%)
 Frame = +3

Query: 321  KSVFLLDNEKLKQIDLYSQFLCPPDKIGVNR--AEGSLERARGLNPMVDVTSHTKGVDEL 494
            K+V  + N KLK ++  S      + +G N      S  +   +  ++ V  H   V EL
Sbjct: 863  KTVESVQNTKLKSVENDSG-KAETETVGKNPEVVRKSPAKLPNIANIMTVNGHRYSVVEL 921

Query: 495  PDSFLR 512
            PD FLR
Sbjct: 922  PDDFLR 927


>SPAC23C11.01 |||ER membrane protein, ICE2
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 441

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 8/18 (44%), Positives = 13/18 (72%)
 Frame = -1

Query: 166 LHYFQPFFAMLLFIISIN 113
           LH+F+P    +L II++N
Sbjct: 116 LHFFEPLLVFILLIIALN 133


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,672,763
Number of Sequences: 5004
Number of extensions: 52854
Number of successful extensions: 187
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)