Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20183
(706 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 75 5e-14
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 66 2e-11
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 51 7e-07
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 49 3e-06
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 49 3e-06
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 49 3e-06
At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative (... 47 1e-05
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 45 6e-05
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 45 6e-05
At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 43 2e-04
At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 40 0.002
At1g05350.1 68414.m00542 thiF family protein low similarity to S... 39 0.003
At5g37530.1 68418.m04520 thiF family protein similar to SP|P3013... 36 0.026
At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3... 33 0.14
At5g54100.1 68418.m06736 band 7 family protein similar to stomat... 29 2.3
At3g22380.1 68416.m02825 expressed protein 28 6.9
At5g07940.1 68418.m00920 expressed protein 27 9.2
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 74.9 bits (176), Expect = 5e-14
Identities = 40/81 (49%), Positives = 55/81 (67%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
S VLI G+ GLGAEIAKN+IL GVKSV L D ++ DL S F+ D +G NRA+ S+
Sbjct: 96 SNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASV 155
Query: 429 ERARGLNPMVDVTSHTKGVDE 491
++ + LN V V+S TK +++
Sbjct: 156 QKLQDLNNAVVVSSLTKSLNK 176
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 66.1 bits (154), Expect = 2e-11
Identities = 37/76 (48%), Positives = 48/76 (63%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
S VLI G+ GLG EIAKN+IL GVKSV L D ++ DL S F+ + IG NRA S+
Sbjct: 93 SNVLISGMQGLGVEIAKNIILAGVKSVTLHDENVVELWDLSSNFVFTEEDIGKNRALASV 152
Query: 429 ERARGLNPMVDVTSHT 476
+ + LN V V++ T
Sbjct: 153 HKLQELNNAVAVSTLT 168
Score = 40.7 bits (91), Expect = 0.001
Identities = 31/119 (26%), Positives = 56/119 (47%), Gaps = 10/119 (8%)
Frame = +3
Query: 219 GLRLAEETTCSQVLIIGLSGLGAEIAKNVILTGVK-----SVFLLDNEKLKQIDLYSQFL 383
G L ++ ++V ++G LG E KN+ L GV + + D++ +++ +L QFL
Sbjct: 480 GSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQFL 539
Query: 384 CPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGV-----DELPDSFLRNLTSYVQQVSN 545
IG ++ + A G+N +++ + V + DSF NLT V + N
Sbjct: 540 FRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDN 598
>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
identical to SUMO activating enzyme 1a [Arabidopsis
thaliana] GI:22652850; contains Pfam profile PF00899:
ThiF family
Length = 322
Score = 51.2 bits (117), Expect = 7e-07
Identities = 30/86 (34%), Positives = 43/86 (50%), Gaps = 3/86 (3%)
Frame = +3
Query: 219 GLRLAEETTCSQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDK 398
G T + +L+ G+ G AE KN++L GV SV L+D+ L + FL PPD+
Sbjct: 22 GANAQRRLTKAHILVSGIKGTVAEFCKNIVLAGVGSVTLMDDRLANMEALNANFLIPPDE 81
Query: 399 ---IGVNRAEGSLERARGLNPMVDVT 467
G AE + + NPMV V+
Sbjct: 82 NVYSGKTVAEICSDSLKDFNPMVRVS 107
Score = 38.3 bits (85), Expect = 0.005
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +1
Query: 169 ELSEAEAEQYDRQIRLWGLDSQKRLRAA 252
EL+E E YDRQIR+WG ++Q+RL A
Sbjct: 5 ELTEQETALYDRQIRVWGANAQRRLTKA 32
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 49.2 bits (112), Expect = 3e-06
Identities = 33/104 (31%), Positives = 49/104 (47%), Gaps = 3/104 (2%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDK---IGVNRAE 419
S VL+ G+ G AE KN++L GV SV LLD+ + + FL PD+ +G AE
Sbjct: 32 SHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAE 91
Query: 420 GSLERARGLNPMVDVTSHTKGVDELPDSFLRNLTSYVQQVSNKS 551
+ + NPMV V+ + L F V S+++
Sbjct: 92 ICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSRA 135
Score = 36.7 bits (81), Expect = 0.015
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +1
Query: 169 ELSEAEAEQYDRQIRLWGLDSQKRL 243
EL+E E YDRQIR+WG +Q+RL
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRL 29
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 49.2 bits (112), Expect = 3e-06
Identities = 33/104 (31%), Positives = 49/104 (47%), Gaps = 3/104 (2%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDK---IGVNRAE 419
S VL+ G+ G AE KN++L GV SV LLD+ + + FL PD+ +G AE
Sbjct: 32 SHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAE 91
Query: 420 GSLERARGLNPMVDVTSHTKGVDELPDSFLRNLTSYVQQVSNKS 551
+ + NPMV V+ + L F V S+++
Sbjct: 92 ICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSRA 135
Score = 36.7 bits (81), Expect = 0.015
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +1
Query: 169 ELSEAEAEQYDRQIRLWGLDSQKRL 243
EL+E E YDRQIR+WG +Q+RL
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRL 29
>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 318
Score = 49.2 bits (112), Expect = 3e-06
Identities = 33/104 (31%), Positives = 49/104 (47%), Gaps = 3/104 (2%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDK---IGVNRAE 419
S VL+ G+ G AE KN++L GV SV LLD+ + + FL PD+ +G AE
Sbjct: 32 SHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAE 91
Query: 420 GSLERARGLNPMVDVTSHTKGVDELPDSFLRNLTSYVQQVSNKS 551
+ + NPMV V+ + L F V S+++
Sbjct: 92 ICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSRA 135
Score = 36.7 bits (81), Expect = 0.015
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +1
Query: 169 ELSEAEAEQYDRQIRLWGLDSQKRL 243
EL+E E YDRQIR+WG +Q+RL
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRL 29
>At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative
(ECR1) identical to putative ubiquitin activating enzyme
E1 [Arabidopsis thaliana] GI:2952433; similar to NEDD8
activating enzyme [Mus musculus] GI:17061821
Length = 454
Score = 47.2 bits (107), Expect = 1e-05
Identities = 21/80 (26%), Positives = 48/80 (60%)
Frame = +3
Query: 252 QVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLE 431
++L+IG GLG E+ K++ L+G +++ ++D ++++ +L QFL + +G +AE + +
Sbjct: 48 RILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAK 107
Query: 432 RARGLNPMVDVTSHTKGVDE 491
R V++ H +++
Sbjct: 108 RVMERVSGVEIVPHFSRIED 127
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 44.8 bits (101), Expect = 6e-05
Identities = 19/79 (24%), Positives = 45/79 (56%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
++VL++G G+G E+ K + L+G + + ++D + ++ +L QFL +G ++A+ +
Sbjct: 13 AKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVAR 72
Query: 429 ERARGLNPMVDVTSHTKGV 485
+ P +++ S+ V
Sbjct: 73 DAVLRFRPNINIRSYHANV 91
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 44.8 bits (101), Expect = 6e-05
Identities = 19/79 (24%), Positives = 45/79 (56%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
++VL++G G+G E+ K + L+G + + ++D + ++ +L QFL +G ++A+ +
Sbjct: 13 AKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVAR 72
Query: 429 ERARGLNPMVDVTSHTKGV 485
+ P +++ S+ V
Sbjct: 73 DAVLRFRPNINIRSYHANV 91
>At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 540
Score = 42.7 bits (96), Expect = 2e-04
Identities = 24/96 (25%), Positives = 49/96 (51%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
+ + ++ G+E KN++L GV S+ ++D K++ DL + F+ +G ++A+
Sbjct: 42 ASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKVQFGDLGNNFMVDAKSVGQSKAKSVC 101
Query: 429 ERARGLNPMVDVTSHTKGVDELPDSFLRNLTSYVQQ 536
+ LN V + K ++E PD+ + S+ Q
Sbjct: 102 AFLQELNDSV----NAKFIEENPDTLITTNPSFFSQ 133
Score = 27.9 bits (59), Expect = 6.9
Identities = 10/31 (32%), Positives = 20/31 (64%)
Frame = +2
Query: 509 TEFDVVCATGLKQEQFERINNACRDSNKKFI 601
++F +V AT L ++ +++ CRD+N K +
Sbjct: 132 SQFTLVIATQLVEDSMLKLDRICRDANVKLV 162
>At2g32410.1 68415.m03960 auxin-resistance protein, putative strong
similarity to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family
Length = 523
Score = 39.5 bits (88), Expect = 0.002
Identities = 23/96 (23%), Positives = 47/96 (48%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
+ + ++ G+E KN+++ G+ S+ ++D K++ DL + F+ +G +RA+
Sbjct: 26 ASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQSRAKTVC 85
Query: 429 ERARGLNPMVDVTSHTKGVDELPDSFLRNLTSYVQQ 536
+ LN V V+E PD+ + S+ Q
Sbjct: 86 GFLQELNDSVKANF----VEENPDTLISTDPSFFSQ 117
>At1g05350.1 68414.m00542 thiF family protein low similarity to
SP|P30138 Adenylyltransferase thiF (EC 2.7.7.-)
{Escherichia coli}; contains Pfam profile PF00899: ThiF
family
Length = 445
Score = 39.1 bits (87), Expect = 0.003
Identities = 21/92 (22%), Positives = 50/92 (54%), Gaps = 2/92 (2%)
Frame = +3
Query: 255 VLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLER 434
V I+G+ G+G+ A+ + G+ + L D + ++ ++ F PD++G+ + + +++
Sbjct: 85 VAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTVELANMNRLFF-RPDQVGMTKTDAAVQT 143
Query: 435 ARGLNPMVDVTSHTKGVDELP--DSFLRNLTS 524
+NP V + S T + + ++F +LT+
Sbjct: 144 LAEINPDVVLESFTMNITTVQGFETFTSSLTN 175
>At5g37530.1 68418.m04520 thiF family protein similar to SP|P30138
Adenylyltransferase thiF (EC 2.7.7.-) {Escherichia
coli}; contains Pfam profile PF00899: ThiF family
Length = 457
Score = 35.9 bits (79), Expect = 0.026
Identities = 18/66 (27%), Positives = 35/66 (53%)
Frame = +3
Query: 219 GLRLAEETTCSQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDK 398
GL + T S V++IGL G+G+ A ++ +GV + L+D +++ L +
Sbjct: 84 GLESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSLNRHAVATRAD 143
Query: 399 IGVNRA 416
+G+ +A
Sbjct: 144 VGIPKA 149
>At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3 /
molybdopterin synthase sulphurylase (CNX5) identical to
SP|Q9ZNW0 Molybdenum cofactor synthesis protein 3
(Molybdopterin synthase sulfurylase) (MPT synthase
sulfurylase) {Arabidopsis thaliana}; contains Pfam
profiles PF00899: ThiF family, PF00581: Rhodanese-like
domain, PF05237: MoeZ/MoeB domain
Length = 464
Score = 33.5 bits (73), Expect = 0.14
Identities = 17/79 (21%), Positives = 39/79 (49%)
Frame = +3
Query: 249 SQVLIIGLSGLGAEIAKNVILTGVKSVFLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSL 428
S VL+IG GLG+ + GV + ++D++ ++ +++ Q + IG + + +
Sbjct: 92 SSVLVIGAGGLGSPALLYLAACGVGQLGIIDHDVVELNNMHRQIIHTEAFIGHPKVKSAA 151
Query: 429 ERARGLNPMVDVTSHTKGV 485
R +N + V + + +
Sbjct: 152 AACRSINSTIKVDEYVEAL 170
>At5g54100.1 68418.m06736 band 7 family protein similar to
stomatin-like protein [Zea mays] GI:7716464; contains
Pfam profile PF01145: SPFH domain / Band 7 family
Length = 401
Score = 29.5 bits (63), Expect = 2.3
Identities = 22/75 (29%), Positives = 36/75 (48%)
Frame = -1
Query: 418 SARFTPILSGGHRNWEYRSICFSFSLSNKNTLFTPVRITFFAISAPSPDKPMINTWLHVV 239
+ RF P+LS S+ + ++ N ++ +P +T + I+ PSP K + LH
Sbjct: 14 AVRFLPMLSAAVPKI-LSSLAAASTVRNFSSTGSP--LTSYQINKPSPSKSFTSRLLHQS 70
Query: 238 SSASLSPTGGFVGRT 194
SSA P F R+
Sbjct: 71 SSAGTPPQQLFGARS 85
>At3g22380.1 68416.m02825 expressed protein
Length = 1550
Score = 27.9 bits (59), Expect = 6.9
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 4/68 (5%)
Frame = -1
Query: 442 PRALSNEPSARFTPILS----GGHRNWEYRSICFSFSLSNKNTLFTPVRITFFAISAPSP 275
P +N +A F+ LS GG N + + + KN F P ++ F + +P+P
Sbjct: 131 PPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNN-FPPTKV-FRSSPSPAP 188
Query: 274 DKPMINTW 251
P+++TW
Sbjct: 189 VSPLVSTW 196
>At5g07940.1 68418.m00920 expressed protein
Length = 1526
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = -1
Query: 433 LSNEPSARFTPILSGGHRNWEYRSICFSFSLSNKNTLFTP 314
L N S F P L G+ NW +R I + S+ + TP
Sbjct: 246 LQNASSNWFQPDLMTGNTNWMHRGISPAVQGSSSGLMITP 285
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,056,821
Number of Sequences: 28952
Number of extensions: 279533
Number of successful extensions: 864
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 863
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -