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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20181X
         (511 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa...    28   0.71 
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb...    27   1.2  
SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|ch...    27   1.2  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   1.6  
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    26   2.9  
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy...    25   5.0  
SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor Fep1|Sc...    25   5.0  
SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc...    25   5.0  
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p...    25   6.6  
SPAC6B12.08 |mug185||DNAJ domain protein Jjj family|Schizosaccha...    25   6.6  
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    25   6.6  
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch...    25   6.6  
SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual    25   8.7  
SPCC285.07c |wtf18||wtf element Wtf18|Schizosaccharomyces pombe|...    25   8.7  

>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 669

 Score = 28.3 bits (60), Expect = 0.71
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = +2

Query: 293 KHDTKPLIISVPNSNYPKNNDLPIPENSELYEDAV 397
           ++D+  L IS    N P+ ND+P+  N + Y+  +
Sbjct: 382 RYDSVDLYISNDKRNLPEYNDVPVVINQDCYDKLI 416


>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1919

 Score = 27.5 bits (58), Expect = 1.2
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -2

Query: 78   DNLVSHLKLVGHLYVVTKMEMHDDYF 1
            DN++   K   HLY+ T +E + D+F
Sbjct: 1055 DNIIHVYKCYQHLYLFTPLETNSDFF 1080


>SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 644

 Score = 27.5 bits (58), Expect = 1.2
 Identities = 21/68 (30%), Positives = 32/68 (47%)
 Frame = +2

Query: 299 DTKPLIISVPNSNYPKNNDLPIPENSELYEDAVAQTSNQQFEDAGFNSHNHASSINSDYS 478
           D  PL+IS P+ N P  +  P PE  E   D   Q      + +  N ++H  S+  + +
Sbjct: 7   DHTPLLISDPSVNKPFRSRTPSPER-EYCSDCPLQA-----KGSACNGNSH--SLKHETN 58

Query: 479 GQSKSSRN 502
           G S S+ N
Sbjct: 59  GASSSNNN 66


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 18/64 (28%), Positives = 27/64 (42%), Gaps = 2/64 (3%)
 Frame = +2

Query: 317  ISVPNSNYPK--NNDLPIPENSELYEDAVAQTSNQQFEDAGFNSHNHASSINSDYSGQSK 490
            +S P S+ P   +N  P P +S +   A A+TS  + +          SS  S  SG+  
Sbjct: 1249 VSTPRSSVPSPHSNASPSPTSSSMASAAPARTSVSRSKSKAERHETSTSSRKSSKSGEHH 1308

Query: 491  SSRN 502
               N
Sbjct: 1309 HHHN 1312


>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 676

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 16/68 (23%), Positives = 28/68 (41%), Gaps = 2/68 (2%)
 Frame = +2

Query: 311 LIISVPNSNYPKNNDLPIPENSELYEDAVAQTSNQQFEDA--GFNSHNHASSINSDYSGQ 484
           LI+ V NSN P     P+  NS       +  SN+   +A    N +   +++N      
Sbjct: 389 LILRVQNSNMPPPAPRPVTNNSSNNNTNQSNASNENSPNAPSAANDNTETTTVNPPQEDD 448

Query: 485 SKSSRNSR 508
              +++ R
Sbjct: 449 QPMTQHER 456


>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2052

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
 Frame = -3

Query: 305  LCHVFPIAYDSCMLIGNFGLMNTSIN-VNNRFIVSKC 198
            +CH  PI    CML G+    N   N V++R +  +C
Sbjct: 1921 MCHKTPILPAICMLCGSVICFNARQNTVSSRRLTGEC 1957


>SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor
           Fep1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 564

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 13/47 (27%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
 Frame = +2

Query: 320 SVPNSNYPKNNDLPIPENSELYEDAVAQTSN-QQFEDAGFNSHNHAS 457
           SVP+S   K +  P+    ++  D   +TSN      +  N H  +S
Sbjct: 116 SVPSSTETKASPTPLESKPQIVSDTTTETSNGTSRRRSSHNQHEDSS 162


>SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 781

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
 Frame = +2

Query: 302 TKPLIISVPNSNYPKNNDLPIPENSE--LYEDAVAQTSNQQFEDAGFNSHNHASSINSDY 475
           +KPL+  V   NY   +    P ++E  ++  AV+ +     +  GF+  N A+  +  Y
Sbjct: 310 SKPLVSRV-RDNYANTSYESWPHSTEFDMFTYAVSGSLKLTPQGTGFDCINPANPFSPGY 368

Query: 476 SGQS 487
           SG+S
Sbjct: 369 SGKS 372


>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 773

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = +2

Query: 407 SNQQFEDAGFNSHNHASSINSDYSGQSKSSRN 502
           S++QF      S N +S  NSD +G+    +N
Sbjct: 79  SSKQFSVTSKRSQNGSSGSNSDANGRKNGQKN 110


>SPAC6B12.08 |mug185||DNAJ domain protein Jjj
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 380

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 15/70 (21%), Positives = 33/70 (47%)
 Frame = +2

Query: 299 DTKPLIISVPNSNYPKNNDLPIPENSELYEDAVAQTSNQQFEDAGFNSHNHASSINSDYS 478
           ++ P++  V N N+   N L   ENS+ ++  + +  NQ+ +     +  +A S      
Sbjct: 266 NSNPIMCMVCNKNFRSQNQLENHENSKKHKKNL-RKMNQEIKKHAKEAQKNAESNKQPED 324

Query: 479 GQSKSSRNSR 508
             S+S  +++
Sbjct: 325 APSESPYSNK 334


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = +2

Query: 368 ENSELYEDAVAQTSNQQFEDAGFNSHNHASSINS 469
           +N+ L+  ++A TS     D GF+  +  S +NS
Sbjct: 18  DNASLFTQSIATTSASTSVDDGFSFDDSISRLNS 51


>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 750

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = +2

Query: 401 QTSNQQFEDAGFNSHNHASSINSDYSGQSKSSRNS 505
           +   Q  + AG    NH S+++SDY  Q KS  +S
Sbjct: 543 ELERQDLKQAG---ENHYSNLSSDYETQIKSLESS 574


>SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 613

 Score = 24.6 bits (51), Expect = 8.7
 Identities = 12/33 (36%), Positives = 23/33 (69%)
 Frame = -2

Query: 123 NKFLRLQLDLRNHIVDNLVSHLKLVGHLYVVTK 25
           +K LRL LD+ +++ + L+SHL +   + +V+K
Sbjct: 261 SKNLRLSLDMFSNVNNQLLSHL-VTSSISIVSK 292


>SPCC285.07c |wtf18||wtf element Wtf18|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 402

 Score = 24.6 bits (51), Expect = 8.7
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 6/38 (15%)
 Frame = -3

Query: 293 FPIAYDSCMLIGNF------GLMNTSINVNNRFIVSKC 198
           + I   S  +IGNF      GL N S+  +NR  +S+C
Sbjct: 290 YEIEASSLFVIGNFLFFYEMGLFNISVIYSNRIRMSRC 327


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,785,551
Number of Sequences: 5004
Number of extensions: 33444
Number of successful extensions: 104
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 204242806
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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