BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20181X (511 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 28 0.71 SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 27 1.2 SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 1.2 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 1.6 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 26 2.9 SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 5.0 SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor Fep1|Sc... 25 5.0 SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 25 5.0 SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 25 6.6 SPAC6B12.08 |mug185||DNAJ domain protein Jjj family|Schizosaccha... 25 6.6 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 6.6 SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 25 6.6 SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual 25 8.7 SPCC285.07c |wtf18||wtf element Wtf18|Schizosaccharomyces pombe|... 25 8.7 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 28.3 bits (60), Expect = 0.71 Identities = 11/35 (31%), Positives = 20/35 (57%) Frame = +2 Query: 293 KHDTKPLIISVPNSNYPKNNDLPIPENSELYEDAV 397 ++D+ L IS N P+ ND+P+ N + Y+ + Sbjct: 382 RYDSVDLYISNDKRNLPEYNDVPVVINQDCYDKLI 416 >SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 27.5 bits (58), Expect = 1.2 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -2 Query: 78 DNLVSHLKLVGHLYVVTKMEMHDDYF 1 DN++ K HLY+ T +E + D+F Sbjct: 1055 DNIIHVYKCYQHLYLFTPLETNSDFF 1080 >SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 27.5 bits (58), Expect = 1.2 Identities = 21/68 (30%), Positives = 32/68 (47%) Frame = +2 Query: 299 DTKPLIISVPNSNYPKNNDLPIPENSELYEDAVAQTSNQQFEDAGFNSHNHASSINSDYS 478 D PL+IS P+ N P + P PE E D Q + + N ++H S+ + + Sbjct: 7 DHTPLLISDPSVNKPFRSRTPSPER-EYCSDCPLQA-----KGSACNGNSH--SLKHETN 58 Query: 479 GQSKSSRN 502 G S S+ N Sbjct: 59 GASSSNNN 66 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.1 bits (57), Expect = 1.6 Identities = 18/64 (28%), Positives = 27/64 (42%), Gaps = 2/64 (3%) Frame = +2 Query: 317 ISVPNSNYPK--NNDLPIPENSELYEDAVAQTSNQQFEDAGFNSHNHASSINSDYSGQSK 490 +S P S+ P +N P P +S + A A+TS + + SS S SG+ Sbjct: 1249 VSTPRSSVPSPHSNASPSPTSSSMASAAPARTSVSRSKSKAERHETSTSSRKSSKSGEHH 1308 Query: 491 SSRN 502 N Sbjct: 1309 HHHN 1312 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 26.2 bits (55), Expect = 2.9 Identities = 16/68 (23%), Positives = 28/68 (41%), Gaps = 2/68 (2%) Frame = +2 Query: 311 LIISVPNSNYPKNNDLPIPENSELYEDAVAQTSNQQFEDA--GFNSHNHASSINSDYSGQ 484 LI+ V NSN P P+ NS + SN+ +A N + +++N Sbjct: 389 LILRVQNSNMPPPAPRPVTNNSSNNNTNQSNASNENSPNAPSAANDNTETTTVNPPQEDD 448 Query: 485 SKSSRNSR 508 +++ R Sbjct: 449 QPMTQHER 456 >SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 2052 Score = 25.4 bits (53), Expect = 5.0 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Frame = -3 Query: 305 LCHVFPIAYDSCMLIGNFGLMNTSIN-VNNRFIVSKC 198 +CH PI CML G+ N N V++R + +C Sbjct: 1921 MCHKTPILPAICMLCGSVICFNARQNTVSSRRLTGEC 1957 >SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor Fep1|Schizosaccharomyces pombe|chr 1|||Manual Length = 564 Score = 25.4 bits (53), Expect = 5.0 Identities = 13/47 (27%), Positives = 21/47 (44%), Gaps = 1/47 (2%) Frame = +2 Query: 320 SVPNSNYPKNNDLPIPENSELYEDAVAQTSN-QQFEDAGFNSHNHAS 457 SVP+S K + P+ ++ D +TSN + N H +S Sbjct: 116 SVPSSTETKASPTPLESKPQIVSDTTTETSNGTSRRRSSHNQHEDSS 162 >SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 781 Score = 25.4 bits (53), Expect = 5.0 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 2/64 (3%) Frame = +2 Query: 302 TKPLIISVPNSNYPKNNDLPIPENSE--LYEDAVAQTSNQQFEDAGFNSHNHASSINSDY 475 +KPL+ V NY + P ++E ++ AV+ + + GF+ N A+ + Y Sbjct: 310 SKPLVSRV-RDNYANTSYESWPHSTEFDMFTYAVSGSLKLTPQGTGFDCINPANPFSPGY 368 Query: 476 SGQS 487 SG+S Sbjct: 369 SGKS 372 >SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces pombe|chr 2|||Manual Length = 773 Score = 25.0 bits (52), Expect = 6.6 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +2 Query: 407 SNQQFEDAGFNSHNHASSINSDYSGQSKSSRN 502 S++QF S N +S NSD +G+ +N Sbjct: 79 SSKQFSVTSKRSQNGSSGSNSDANGRKNGQKN 110 >SPAC6B12.08 |mug185||DNAJ domain protein Jjj family|Schizosaccharomyces pombe|chr 1|||Manual Length = 380 Score = 25.0 bits (52), Expect = 6.6 Identities = 15/70 (21%), Positives = 33/70 (47%) Frame = +2 Query: 299 DTKPLIISVPNSNYPKNNDLPIPENSELYEDAVAQTSNQQFEDAGFNSHNHASSINSDYS 478 ++ P++ V N N+ N L ENS+ ++ + + NQ+ + + +A S Sbjct: 266 NSNPIMCMVCNKNFRSQNQLENHENSKKHKKNL-RKMNQEIKKHAKEAQKNAESNKQPED 324 Query: 479 GQSKSSRNSR 508 S+S +++ Sbjct: 325 APSESPYSNK 334 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.0 bits (52), Expect = 6.6 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = +2 Query: 368 ENSELYEDAVAQTSNQQFEDAGFNSHNHASSINS 469 +N+ L+ ++A TS D GF+ + S +NS Sbjct: 18 DNASLFTQSIATTSASTSVDDGFSFDDSISRLNS 51 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 25.0 bits (52), Expect = 6.6 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +2 Query: 401 QTSNQQFEDAGFNSHNHASSINSDYSGQSKSSRNS 505 + Q + AG NH S+++SDY Q KS +S Sbjct: 543 ELERQDLKQAG---ENHYSNLSSDYETQIKSLESS 574 >SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual Length = 613 Score = 24.6 bits (51), Expect = 8.7 Identities = 12/33 (36%), Positives = 23/33 (69%) Frame = -2 Query: 123 NKFLRLQLDLRNHIVDNLVSHLKLVGHLYVVTK 25 +K LRL LD+ +++ + L+SHL + + +V+K Sbjct: 261 SKNLRLSLDMFSNVNNQLLSHL-VTSSISIVSK 292 >SPCC285.07c |wtf18||wtf element Wtf18|Schizosaccharomyces pombe|chr 3|||Manual Length = 402 Score = 24.6 bits (51), Expect = 8.7 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 6/38 (15%) Frame = -3 Query: 293 FPIAYDSCMLIGNF------GLMNTSINVNNRFIVSKC 198 + I S +IGNF GL N S+ +NR +S+C Sbjct: 290 YEIEASSLFVIGNFLFFYEMGLFNISVIYSNRIRMSRC 327 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,785,551 Number of Sequences: 5004 Number of extensions: 33444 Number of successful extensions: 104 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 101 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 104 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 204242806 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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