Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20177
         (609 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo...    30   0.30 
SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|ch...    29   0.53 
SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos...    28   1.2  
SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase Nak1|Schizosacch...    28   1.2  
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    27   2.1  
SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual         26   3.7  
SPAC30.03c |tsn1|tsn, mug90|translin|Schizosaccharomyces pombe|c...    26   3.7  
SPAC1F8.04c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Manual     26   3.7  
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch...    26   3.7  
SPBC336.08 |spc24||spindle pole body protein Spc24|Schizosacchar...    25   6.5  
SPBPB8B6.06c ||SPAPB8B6.06c, SPAPB8B6.06c|conserved fungal prote...    25   8.7  
SPBC19F8.02 |||nuclear distribution protein NUDC |Schizosaccharo...    25   8.7  
SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|...    25   8.7  
SPAC977.11 |||conserved fungal protein|Schizosaccharomyces pombe...    25   8.7  
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo...    25   8.7  

>SPBC12C2.02c |ste20|ste16|sterility protein
           Ste20|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1309

 Score = 29.9 bits (64), Expect = 0.30
 Identities = 25/75 (33%), Positives = 38/75 (50%), Gaps = 8/75 (10%)
 Frame = +3

Query: 258 KMLGEQVKKMLMALDKKHRMLEPLKGMISRLDQRLSNVETIL------LQKE--EREKGS 413
           K  G+ +KKM     K+ ++LE LKG +    +     ET+L       +KE  ER++  
Sbjct: 20  KTNGDFIKKMNTTDSKRLKLLEDLKGKLEVECKIRDGAETLLQVFDTNFKKETKERKEML 79

Query: 414 XKKTDEALESIQKSI 458
            KK  + LES +K I
Sbjct: 80  KKKCTDELESSKKKI 94


>SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 979

 Score = 29.1 bits (62), Expect = 0.53
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = +3

Query: 333 GMISRLDQRLSNVETILLQKEEREKGSXKKTD 428
           G I  +D +  ++E  L ++EER+KG  KK++
Sbjct: 110 GSIEHVDHQNISLEASLAEEEERKKGDAKKSE 141


>SPBC1539.05 |cog3||Golgi transport complex subunit Cog3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 14/47 (29%), Positives = 26/47 (55%)
 Frame = +3

Query: 300 DKKHRMLEPLKGMISRLDQRLSNVETILLQKEEREKGSXKKTDEALE 440
           +KK   L+    +I ++  + +N+E+ LL  +E+ + S KK  E  E
Sbjct: 102 EKKINELQTFTNIIDQILGQTNNIESTLLSMKEKFESSEKKLSEFSE 148


>SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase
           Nak1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 652

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 19/52 (36%), Positives = 24/52 (46%)
 Frame = -3

Query: 223 LLAPNELRSSAHPGYPRL*LPGILFEESTIEARRRPTIV*FRSACRPFNSKL 68
           +L P E+  S     P+   P IL + S   A   P I    S  +PFNSKL
Sbjct: 522 VLQPLEVNRSISIPPPKSISPSILHKPSLESASSTPKISSCSSTPKPFNSKL 573


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 29/138 (21%), Positives = 64/138 (46%)
 Frame = +3

Query: 75   ELKGRHAERNQTMVGRRRASIVDSSNRMPGSYNRGYPGCADDLSSFGASNRRQTGET*VQ 254
            +LK + +E + T +   ++ +    N++  + N      AD+LSS  + N+ Q  E    
Sbjct: 803  DLKNKLSEES-TRLQELQSQLNQDKNQIE-TLNERISAAADELSSMESINKNQANEL--- 857

Query: 255  EKMLGEQVKKMLMALDKKHRMLEPLKGMISRLDQRLSNVETILLQKEEREKGSXKKTDEA 434
             K+  ++   +   ++  +++ +     IS L++ L    T       +E  + K  +++
Sbjct: 858  -KLAKQKCSNLQEKINFGNKLAKEHTEKISSLEKDL-EAATKTASTLSKELKTVKSENDS 915

Query: 435  LESIQKSIPSSNDDSHRE 488
            L+S+      SNDD ++E
Sbjct: 916  LKSV------SNDDQNKE 927


>SPCC1450.15 |||pig-F |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 503

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 13/41 (31%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
 Frame = -3

Query: 376 VSTFESL*SSLDIIPFSGSNILCFLSSAISIFFT-CSPSIF 257
           ++++ES+ S ++ +PF   +++    S I  FFT   PS+F
Sbjct: 84  LTSYESVHSMIERLPFCPDHVVHCAGSCIPGFFTELDPSVF 124


>SPAC30.03c |tsn1|tsn, mug90|translin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 236

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 11/47 (23%), Positives = 25/47 (53%)
 Frame = +3

Query: 297 LDKKHRMLEPLKGMISRLDQRLSNVETILLQKEEREKGSXKKTDEAL 437
           +DK+H + E L   +  LD++L  ++ +L   E+    + ++ +  L
Sbjct: 13  IDKEHSIREKLTAEVDLLDEKLRVLQLLLANCEQSRNENLQEKEHGL 59


>SPAC1F8.04c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 463

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = +1

Query: 142 TPQTECREVTIEDIRDVLMTLVHLVRATEDKLERH 246
           TP T C+++ +   + VL  +VHL     + LE+H
Sbjct: 247 TPMTYCKDLGLLGPKTVLAHMVHLDTQDLEILEKH 281


>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 750

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 11/47 (23%), Positives = 25/47 (53%)
 Frame = +3

Query: 306 KHRMLEPLKGMISRLDQRLSNVETILLQKEEREKGSXKKTDEALESI 446
           +  +    +  I +L+  +SN  +++LQK++  + S ++  E  E I
Sbjct: 121 RENLKHQFEDQIEKLNSEISNQNSLILQKKDELEKSIQRCSELEEKI 167


>SPBC336.08 |spc24||spindle pole body protein
           Spc24|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 198

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 20/73 (27%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
 Frame = +3

Query: 198 DLSSFGASNRRQTGET*VQ-EKMLGEQVKKMLMALDKKHRMLEPLKGMISRLDQRLSNVE 374
           ++S+    + +QT E+    E++L  + +K  +A     ++L   +  I  L+ +L  ++
Sbjct: 58  EISTQAMESLKQTAESSQHAEEILSREKEKFRIA-----KLLNITENEIMSLESQLQKMK 112

Query: 375 TILLQKEEREKGS 413
             LLQ EERE  S
Sbjct: 113 EQLLQLEERENTS 125


>SPBPB8B6.06c ||SPAPB8B6.06c, SPAPB8B6.06c|conserved fungal
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 311

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = -3

Query: 400 RSSFCRRIVSTFESL*SSLDIIPFSGSNILCFLSSAISIFFTC 272
           ++ FC  ++ST  +  + L  +P   + I C +S AIS F  C
Sbjct: 254 QNGFCA-VLSTLSTFSNELHTMPIKRAYIYCIISVAIS-FSIC 294


>SPBC19F8.02 |||nuclear distribution protein NUDC
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 14/58 (24%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
 Frame = +3

Query: 318 LEPLKGMISRLDQRL-SNVETILLQKEEREKGSXKKTDEALESIQKSIPSSNDDSHRE 488
           +EP    +S LD+   + VE ++L++ ++     K+ D  L++  K  P  +  + R+
Sbjct: 108 IEPENSKLSDLDEETRATVEKMMLEQSQKRTDEQKRKD-VLQNFMKQHPELDFSNVRD 164


>SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 767

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/39 (28%), Positives = 20/39 (51%)
 Frame = +1

Query: 160 REVTIEDIRDVLMTLVHLVRATEDKLERHEFRRRCLGNK 276
           + V I+D+ D L +++ + R  EDK     F  + L  +
Sbjct: 430 KNVDIDDVEDCLSSIIIIFRYVEDKDVFQNFYTKLLAKR 468


>SPAC977.11 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 311

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = -3

Query: 400 RSSFCRRIVSTFESL*SSLDIIPFSGSNILCFLSSAISIFFTC 272
           ++ FC  ++ST  +  + L  +P   + I C +S AIS F  C
Sbjct: 254 QNGFCA-VLSTLSTFSNELHTMPIKRAYIYCIISVAIS-FSIC 294


>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1183

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
 Frame = +3

Query: 351 DQRLSNVETIL-LQKEEREKGSXKKTDEALESIQKSIPS 464
           D + SN E ++ + KEER+K   +  D+ L+  + SI S
Sbjct: 48  DNQDSNAEILIDVPKEERQKRKQELKDQLLKENEGSISS 86


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,047,281
Number of Sequences: 5004
Number of extensions: 37766
Number of successful extensions: 124
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)