Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20168
(497 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 133 7e-32
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 132 2e-31
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 6e-31
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 111 2e-25
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 67 7e-12
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 67 9e-12
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 46 2e-05
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 4e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.53
SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 0.92
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.2
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 1.6
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 3.7
SB_42630| Best HMM Match : Ets (HMM E-Value=0) 28 4.9
SB_34715| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 4.9
SB_31018| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 4.9
SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14) 28 4.9
SB_46753| Best HMM Match : Fimbrial_CS1 (HMM E-Value=1.1) 27 8.6
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 133 bits (322), Expect = 7e-32
Identities = 61/83 (73%), Positives = 73/83 (87%)
Frame = +3
Query: 249 PQNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 428
P+NTIFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 429 VLTKMKETAEAYLGKTVQNAVIT 497
VL KMKETAEAYLG+ V +AVIT
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVIT 145
Score = 127 bits (307), Expect = 4e-30
Identities = 57/64 (89%), Positives = 62/64 (96%)
Frame = +1
Query: 64 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 243
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 244 MNPK 255
+NP+
Sbjct: 61 LNPE 64
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 132 bits (319), Expect = 2e-31
Identities = 61/64 (95%), Positives = 63/64 (98%)
Frame = +1
Query: 64 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 243
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 244 MNPK 255
MNP+
Sbjct: 61 MNPE 64
Score = 132 bits (319), Expect = 2e-31
Identities = 58/83 (69%), Positives = 70/83 (84%)
Frame = +3
Query: 249 PQNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 428
P+NT+FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 429 VLTKMKETAEAYLGKTVQNAVIT 497
VLTKMKETAEAYLG V +AV+T
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVT 145
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 130 bits (314), Expect = 6e-31
Identities = 59/63 (93%), Positives = 61/63 (96%)
Frame = +1
Query: 64 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 243
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 244 MNP 252
MNP
Sbjct: 61 MNP 63
Score = 108 bits (259), Expect = 3e-24
Identities = 61/108 (56%), Positives = 70/108 (64%), Gaps = 25/108 (23%)
Frame = +3
Query: 249 PQNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV--- 419
P NTIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 420 ----------------------SSMVLTKMKETAEAYLGKTVQNAVIT 497
SSMVL KMKETAEAYLG V +AV+T
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVT 170
Score = 43.2 bits (97), Expect = 1e-04
Identities = 20/28 (71%), Positives = 24/28 (85%)
Frame = +3
Query: 414 EVSSMVLTKMKETAEAYLGKTVQNAVIT 497
++SSMVL KMKETAEAYLG V +AV+T
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVT 238
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 111 bits (268), Expect = 2e-25
Identities = 52/83 (62%), Positives = 66/83 (79%)
Frame = +3
Query: 249 PQNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 428
P+NT+FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 429 VLTKMKETAEAYLGKTVQNAVIT 497
VLTKMKETAEAYLGK V +AV+T
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVT 843
Score = 100 bits (239), Expect = 8e-22
Identities = 46/59 (77%), Positives = 53/59 (89%), Gaps = 1/59 (1%)
Frame = +1
Query: 82 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPK 255
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP+
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPE 762
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 67.3 bits (157), Expect = 7e-12
Identities = 30/64 (46%), Positives = 44/64 (68%), Gaps = 2/64 (3%)
Frame = +1
Query: 70 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 243
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 244 MNPK 255
+NPK
Sbjct: 90 INPK 93
Score = 53.2 bits (122), Expect = 1e-07
Identities = 31/84 (36%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
Frame = +3
Query: 249 PQNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSD-GGKPKIKVAYKGEDKTFFPEEVSS 425
P+NTI+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 426 MVLTKMKETAEAYLGKTVQNAVIT 497
V+ K++ AE L TV AV++
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMS 175
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 66.9 bits (156), Expect = 9e-12
Identities = 35/83 (42%), Positives = 50/83 (60%), Gaps = 1/83 (1%)
Frame = +3
Query: 252 QNTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSM 428
+NTI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M
Sbjct: 120 KNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAM 179
Query: 429 VLTKMKETAEAYLGKTVQNAVIT 497
+ T++K TAE L V + VI+
Sbjct: 180 LFTRLKTTAEIALKTKVTDCVIS 202
Score = 49.6 bits (113), Expect = 1e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +1
Query: 82 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 237
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 46.0 bits (104), Expect = 2e-05
Identities = 21/43 (48%), Positives = 32/43 (74%)
Frame = +3
Query: 369 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVIT 497
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVIT
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVIT 44
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 4e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +1
Query: 133 GKVEIIANDQGNRTTPSYVAFTDTERLI 216
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.53
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +1
Query: 142 EIIANDQGNRTTPSYVAFT-DTERL 213
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 665
Score = 30.3 bits (65), Expect = 0.92
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = +3
Query: 51 LKIQNGKSTRSRN-RSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCR 227
L++ N + ++ + R GY L R C R + +R + H++L R +RC
Sbjct: 225 LRLVNTRDDKTHDKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTETETRFCAKRCA 282
Query: 228 QEPGGDEPQNTIFDA 272
G +P+NT F+A
Sbjct: 283 GTASGMDPKNTPFNA 297
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 29.9 bits (64), Expect = 1.2
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +3
Query: 363 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVIT 497
P +K Y+ D TF PEE+ M+L + E + +++ V+T
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLT 111
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -2
Query: 250 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 152
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.3 bits (60), Expect = 3.7
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -2
Query: 457 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRP 284
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165
>SB_42630| Best HMM Match : Ets (HMM E-Value=0)
Length = 631
Score = 27.9 bits (59), Expect = 4.9
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +2
Query: 92 IWVPRTLALVSSSTGRWRSSPTTRATGPLRLMLRSQTPSVSSEMPPRT 235
IW P+ L++S R+S + PLR L P VSS +P T
Sbjct: 42 IWPPKKQHLMASYPSTSRASASRSHLFPLRTGLPPVPPLVSSPLPSPT 89
>SB_34715| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 977
Score = 27.9 bits (59), Expect = 4.9
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +2
Query: 92 IWVPRTLALVSSSTGRWRSSPTTRATGPLRLMLRSQTPSVSSEMPPRT 235
IW P+ L++S R+S + PLR L P VSS +P T
Sbjct: 388 IWPPKKQHLMASYPSTSRASASRSHLFPLRTGLPPVPPLVSSPLPSPT 435
>SB_31018| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1066
Score = 27.9 bits (59), Expect = 4.9
Identities = 20/75 (26%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Frame = +3
Query: 51 LKIQNGKSTRSRN-RSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCR 227
L++ N + ++ R GY L R C R + +R + H++L R +RC
Sbjct: 661 LRLVNTRDDKTHGKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTDTETRFCEKRCA 718
Query: 228 QEPGGDEPQNTIFDA 272
G +P+N F+A
Sbjct: 719 GTASGMDPKNIPFNA 733
>SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14)
Length = 856
Score = 27.9 bits (59), Expect = 4.9
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = -1
Query: 260 CVLGFIATWFLAASPMRRSVSVNAT*DGVVLLPWSLAMISTFPCWKTPTQEYVVPRSIPT 81
C+L I WF P+R S + VV+ S+ ++ F C P + P +IP+
Sbjct: 107 CLLLTIERWFAVVQPLRYQASFSKR--RVVVSLISVYCVACFLCCTLPFEVSFSPENIPS 164
>SB_46753| Best HMM Match : Fimbrial_CS1 (HMM E-Value=1.1)
Length = 982
Score = 27.1 bits (57), Expect = 8.6
Identities = 17/53 (32%), Positives = 22/53 (41%), Gaps = 1/53 (1%)
Frame = +1
Query: 145 IIAND-QGNRTTPSYVAFTDTERLIGDAAKNQVAMNPKTQYSMPNVSSDVSSK 300
+I ND + PS V D R K + KTQ + PN+ S S K
Sbjct: 209 VIMNDITSHYPIPSMVIMNDITRHCAKGVKTHGYIGKKTQATTPNIPSSSSVK 261
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,848,133
Number of Sequences: 59808
Number of extensions: 426262
Number of successful extensions: 1255
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1248
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1075029208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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