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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20166
         (548 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g72320.3 68414.m08362 pumilio/Puf RNA-binding domain-containi...    32   0.29 
At1g72320.2 68414.m08361 pumilio/Puf RNA-binding domain-containi...    32   0.29 
At1g72320.1 68414.m08363 pumilio/Puf RNA-binding domain-containi...    32   0.29 
At5g41780.1 68418.m05087 myosin heavy chain-related weak similar...    30   0.89 
At2g28350.1 68415.m03445 auxin-responsive factor (ARF10) similar...    29   2.0  
At3g28850.1 68416.m03599 glutaredoxin family protein                   29   2.7  
At5g40940.1 68418.m04971 hypothetical protein                          27   6.2  
At1g26870.1 68414.m03277 no apical meristem (NAM) family protein...    27   8.3  

>At1g72320.3 68414.m08362 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile: PF00806 Pumilio-family
           RNA binding domains (aka PUM-HD, Pumilio homology
           domain) (5 copies)
          Length = 731

 Score = 31.9 bits (69), Expect = 0.29
 Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
 Frame = -1

Query: 404 GFDFNSRASPQSPS--DIKRESRSFNTSLSSPRLIGRYLDPRNHSYYEKVLTVQDNNNIK 231
           GFD +S    QS    ++K  S+  +      + + + +DP    Y+ ++  + D+N ++
Sbjct: 28  GFDVDSSKKNQSGGAPNVKPASKKHSEFEHQNQFVRKEIDPETSKYFSEIANLFDSNEVE 87

Query: 230 L-RREVCC 210
           L  R V C
Sbjct: 88  LEERSVIC 95


>At1g72320.2 68414.m08361 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile: PF00806 Pumilio-family
           RNA binding domains (aka PUM-HD, Pumilio homology
           domain) (5 copies)
          Length = 731

 Score = 31.9 bits (69), Expect = 0.29
 Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
 Frame = -1

Query: 404 GFDFNSRASPQSPS--DIKRESRSFNTSLSSPRLIGRYLDPRNHSYYEKVLTVQDNNNIK 231
           GFD +S    QS    ++K  S+  +      + + + +DP    Y+ ++  + D+N ++
Sbjct: 28  GFDVDSSKKNQSGGAPNVKPASKKHSEFEHQNQFVRKEIDPETSKYFSEIANLFDSNEVE 87

Query: 230 L-RREVCC 210
           L  R V C
Sbjct: 88  LEERSVIC 95


>At1g72320.1 68414.m08363 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile: PF00806 Pumilio-family
           RNA binding domains (aka PUM-HD, Pumilio homology
           domain) (5 copies)
          Length = 753

 Score = 31.9 bits (69), Expect = 0.29
 Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
 Frame = -1

Query: 404 GFDFNSRASPQSPS--DIKRESRSFNTSLSSPRLIGRYLDPRNHSYYEKVLTVQDNNNIK 231
           GFD +S    QS    ++K  S+  +      + + + +DP    Y+ ++  + D+N ++
Sbjct: 50  GFDVDSSKKNQSGGAPNVKPASKKHSEFEHQNQFVRKEIDPETSKYFSEIANLFDSNEVE 109

Query: 230 L-RREVCC 210
           L  R V C
Sbjct: 110 LEERSVIC 117


>At5g41780.1 68418.m05087 myosin heavy chain-related weak similarity
           to  M protein, serotype 5 precursor (SP:P02977)
           {Streptococcus pyogenes} and to Myosin heavy chain,
           non-muscle (SP:Q99323) (Zipper protein) (Myosin II)
           {Drosophila melanogaster}
          Length = 537

 Score = 30.3 bits (65), Expect = 0.89
 Identities = 16/42 (38%), Positives = 26/42 (61%)
 Frame = +1

Query: 310 KRGEDREVLKDRLSRLMSDGDCGEARELKSKPVEKPAKKTAT 435
           KR EDRE LK  +++L +   C + REL+ + V+K  + + T
Sbjct: 143 KRNEDREHLKGLMTKLEAALLCNQKRELEMELVKKTNQVSET 184


>At2g28350.1 68415.m03445 auxin-responsive factor (ARF10) similar to
           auxin response factor 10 GI:6165644 from [Arabidopsis
           thaliana]; identical to cDNA auxin response factor 10
           (ARF10) mRNA, partial cds GI:6165643
          Length = 693

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 16/49 (32%), Positives = 25/49 (51%)
 Frame = -1

Query: 380 SPQSPSDIKRESRSFNTSLSSPRLIGRYLDPRNHSYYEKVLTVQDNNNI 234
           SP   SD+   S + N  L SP +     +PR+H Y  +    +++NNI
Sbjct: 459 SPSLLSDLNLSSYTGNNKLHSPAMFLSSFNPRHHHYQAR--DSENSNNI 505


>At3g28850.1 68416.m03599 glutaredoxin family protein 
          Length = 428

 Score = 28.7 bits (61), Expect = 2.7
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = +3

Query: 408 RKTSEENCDITVPIKKCGMKV 470
           RKT EE+CD+ V +K  G++V
Sbjct: 264 RKTYEESCDVRVILKSLGIRV 284


>At5g40940.1 68418.m04971 hypothetical protein
          Length = 424

 Score = 27.5 bits (58), Expect = 6.2
 Identities = 12/28 (42%), Positives = 15/28 (53%)
 Frame = -1

Query: 134 TSSTTFLPISKKIPDPINPFSNNSTFIR 51
           T  T F PI + IP+P   FS+  T  R
Sbjct: 289 TKITVFAPIDEAIPNPTTKFSDYVTIFR 316


>At1g26870.1 68414.m03277 no apical meristem (NAM) family protein
           contains Pfam PF02365: No apical meristem (NAM) domain;
           similar to GB:AAD22369, NAM stands for No Apicla
           Meristem
          Length = 425

 Score = 27.1 bits (57), Expect = 8.3
 Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 4/42 (9%)
 Frame = -1

Query: 158 FLTNIFILTSSTT----FLPISKKIPDPINPFSNNSTFIRMF 45
           +LTN+F+ T  T      LP S +IP  +   S++STF+  F
Sbjct: 282 YLTNLFLATQETQPQFPRLPSSNEIPSFLLNTSSDSTFLGEF 323


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,546,714
Number of Sequences: 28952
Number of extensions: 199316
Number of successful extensions: 631
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 631
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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