BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20159 (609 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 28 1.2 SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schi... 27 2.1 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 27 2.8 SPCC18B5.05c |||phosphomethylpyrimidine kinase |Schizosaccharomy... 26 3.7 SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 3.7 SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pom... 26 5.0 SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc... 25 6.5 SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 25 6.5 SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 25 6.5 SPAC5D6.10c |mug116||sequence orphan|Schizosaccharomyces pombe|c... 25 6.5 SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 25 8.7 >SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 1052 Score = 27.9 bits (59), Expect = 1.2 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = -3 Query: 244 ISNTVELPGLRSSVVKVLKSTLAACGKRRS 155 IS LP L+S++V ++STL CG + S Sbjct: 430 ISTIYILPFLKSTIVSSVQSTLQVCGAQTS 459 >SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 377 Score = 27.1 bits (57), Expect = 2.1 Identities = 18/56 (32%), Positives = 31/56 (55%), Gaps = 2/56 (3%) Frame = -1 Query: 513 NDFLLSLINLNVSFLASYRYILGGMLQALHVTMA*L--APGENMADTCLQVGSLHE 352 ND L SL+++ VS S + ++ L+V + L AP E + CLQ+ +++E Sbjct: 53 NDVLFSLLDVAVSI--SSLELRKELISELYVPVQSLEEAPSEYLVSCCLQLATIYE 106 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 26.6 bits (56), Expect = 2.8 Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 2/69 (2%) Frame = +2 Query: 266 NTVYYIVAPGNLPWLRQIWATSGGVVRCISCKEPTCRHVSAMFSPGANYAIVTCSACNI- 442 N +YYI++ GN W+ V+ KE H S MF NYAI+T S I Sbjct: 538 NKIYYILSKGNY---FVDWSICDEYVKRF--KED---HFSFMFKKEENYAIITLSLVKIA 589 Query: 443 -PPKIYLYD 466 P LY+ Sbjct: 590 STPMSKLYE 598 >SPCC18B5.05c |||phosphomethylpyrimidine kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 327 Score = 26.2 bits (55), Expect = 3.7 Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 1/39 (2%) Frame = +2 Query: 413 AIVTCSACNIPPKIYLYDAKKE-TFKLIKDNKKSLVPYK 526 AI + ACNIPP +++A + + + +KKS PYK Sbjct: 247 AIASNLACNIPPLQAIHEALYSIEWAIQRVHKKSSDPYK 285 >SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 630 Score = 26.2 bits (55), Expect = 3.7 Identities = 14/39 (35%), Positives = 18/39 (46%) Frame = +2 Query: 371 CRHVSAMFSPGANYAIVTCSACNIPPKIYLYDAKKETFK 487 C SA+ N C AC I P L+DAK+ T + Sbjct: 144 CILCSALEKINVNELFYMCRACVIYPITPLWDAKERTMR 182 >SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 577 Score = 25.8 bits (54), Expect = 5.0 Identities = 15/47 (31%), Positives = 21/47 (44%) Frame = +2 Query: 416 IVTCSACNIPPKIYLYDAKKETFKLIKDNKKSLVPYKCTNYLLPIFI 556 I TC P + L + TF++ K K S+ P T YL +I Sbjct: 524 IATCDKPEDPHQFKLQSGDQNTFRISKKKKPSICPIAYT-YLWHAYI 569 >SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Schizosaccharomyces pombe|chr 3|||Manual Length = 363 Score = 25.4 bits (53), Expect = 6.5 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +3 Query: 327 LPAVWLDVSRAKNRLVGTC 383 L W+DV + K+ L GTC Sbjct: 213 LEGYWMDVGQPKDYLTGTC 231 >SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1588 Score = 25.4 bits (53), Expect = 6.5 Identities = 19/58 (32%), Positives = 31/58 (53%), Gaps = 4/58 (6%) Frame = +2 Query: 311 RQIWATSGGVVRCISCKE---PTCRHVSA-MFSPGANYAIVTCSACNIPPKIYLYDAK 472 RQ +A S G V+C +C E C +S+ + S +NYA C C+ K+Y ++ + Sbjct: 1460 RQPFAISDGTVQCHNCLEWFHYECVGLSSDIVSTLSNYACPDC--CSKEGKLYPWNTR 1515 >SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr 3|||Manual Length = 1647 Score = 25.4 bits (53), Expect = 6.5 Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = -3 Query: 247 TISNTVELPGLRSSVVKVLKSTLAACGKRRSA*YKGCISTILVPVL-SKIGSLMSIQPFG 71 +++ V L L S + LKS L + + + K CI TI P+L +K+ ++ + G Sbjct: 136 SLTRVVGLKDLESDISTALKSYLQSLCENGNNLVKTCIDTI--PILDNKVSEILKSEAGG 193 Query: 70 SV 65 + Sbjct: 194 QI 195 >SPAC5D6.10c |mug116||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 135 Score = 25.4 bits (53), Expect = 6.5 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +3 Query: 432 PATSLLKYTCMMPRKRHSN*LKTTKNHWYPT 524 P SL KYTC++P L T +H + T Sbjct: 52 PTFSLSKYTCVLPASIPEMILFTFSSHTFQT 82 >SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces pombe|chr 2|||Manual Length = 241 Score = 25.0 bits (52), Expect = 8.7 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +2 Query: 458 LYDAKKETFKLIKDNKKSLVPYKCTNY 538 L KKE KL DN++S +P+ T + Sbjct: 106 LLTTKKEHKKLDNDNRESEIPFSDTRF 132 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,786,164 Number of Sequences: 5004 Number of extensions: 62109 Number of successful extensions: 169 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 169 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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