BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20154 (421 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC530.10c |anc1||adenine nucleotide carrier Anc1|Schizosacchar... 52 4e-08 SPAPB17E12.12c |||mitochondrial transporter|Schizosaccharomyces ... 34 0.010 SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizos... 30 0.13 SPAC19G12.05 |||mitochondrial citrate transporter|Schizosaccharo... 27 0.90 SPAC328.09 |||2-oxoadipate and 2-oxoglutarate transporter |Schiz... 27 1.2 SPAC227.03c |||mitochondrial NAD+ transporter|Schizosaccharomyce... 27 1.2 SPAC688.09 |||pyrimidine nucletide transporter |Schizosaccharomy... 27 1.6 SPBC12D12.05c |||mitochondrial carrier, calcium binding subfamil... 26 2.1 SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te... 24 8.4 SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces pombe... 24 8.4 >SPBC530.10c |anc1||adenine nucleotide carrier Anc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 322 Score = 52.0 bits (119), Expect = 4e-08 Identities = 26/53 (49%), Positives = 34/53 (64%), Gaps = 1/53 (1%) Frame = +1 Query: 256 RVKLLLQVQ-HVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNLANVIRXF 411 RVKLL+Q Q + + RYKGI + F R E+G++S WRGN ANV+R F Sbjct: 50 RVKLLIQNQDEMIRAGRLSHRYKGIGECFKRTAAEEGVISLWRGNTANVLRYF 102 Score = 28.7 bits (61), Expect = 0.39 Identities = 13/22 (59%), Positives = 15/22 (68%) Frame = +2 Query: 188 FAKDFLAGGISXXGSKTAVAPI 253 F DF+ GG+S SKTA API Sbjct: 27 FFFDFMMGGVSAAVSKTAAAPI 48 >SPAPB17E12.12c |||mitochondrial transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 33.9 bits (74), Expect = 0.010 Identities = 17/52 (32%), Positives = 29/52 (55%) Frame = +1 Query: 256 RVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNLANVIRXF 411 R+K++ QVQ+ ++ Y + V+I +GL+ F+RGN N +R F Sbjct: 43 RMKIIFQVQN-------NKEYTSLTSTLVKIWNREGLIGFFRGNGTNCLRAF 87 Score = 26.2 bits (55), Expect = 2.1 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +1 Query: 316 YKGIVDAFVRIPKEQGLLSFWRGNLANVIR 405 YK I A I K +G+ F+RG +N+++ Sbjct: 266 YKNIKSAIFHIYKTEGINGFFRGYSSNMLK 295 >SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 30.3 bits (65), Expect = 0.13 Identities = 11/30 (36%), Positives = 20/30 (66%) Frame = +1 Query: 196 GLPGWXYLRRRLQDRRSTNRRVKLLLQVQH 285 G PG+ ++R LQD+R NR ++++ + H Sbjct: 46 GTPGFIAVKRVLQDKRYKNRELQIMRAISH 75 >SPAC19G12.05 |||mitochondrial citrate transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 291 Score = 27.5 bits (58), Expect = 0.90 Identities = 11/38 (28%), Positives = 19/38 (50%) Frame = +1 Query: 292 KQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNLANVIR 405 + ++A + YK + +I + GLL FW G + R Sbjct: 232 QSLSASKEYKNSIHCAYKILTQDGLLRFWSGATPRLAR 269 >SPAC328.09 |||2-oxoadipate and 2-oxoglutarate transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 298 Score = 27.1 bits (57), Expect = 1.2 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +1 Query: 313 RYKGIVDAFVRIPKEQGLLSFWRGNLANVIR 405 +Y G VD F +I K++ +L+ + G A + R Sbjct: 140 KYNGTVDCFTKIVKQERILALYNGFEATMWR 170 >SPAC227.03c |||mitochondrial NAD+ transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 27.1 bits (57), Expect = 1.2 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +1 Query: 301 AADQRYKGIVDAFVRIPKEQGLLSFWRG 384 A Y DAF +I K +GL +F+RG Sbjct: 225 ACSPAYNNTFDAFRKIYKYEGLAAFYRG 252 >SPAC688.09 |||pyrimidine nucletide transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 26.6 bits (56), Expect = 1.6 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = +1 Query: 289 SKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNLANVIR 405 S I +Y G++ F + EQG++ + G A+++R Sbjct: 303 SPSINGTPKYTGLIQCFKLVWMEQGIVGLYGGLTAHLLR 341 >SPBC12D12.05c |||mitochondrial carrier, calcium binding subfamily|Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 26.2 bits (55), Expect = 2.1 Identities = 13/49 (26%), Positives = 23/49 (46%) Frame = +1 Query: 259 VKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNLANVIR 405 ++ LQ Q S A Y G +D F + K +G ++G N+++ Sbjct: 359 IRTRLQTQGTSAHPAT---YDGFIDCFYKTTKNEGFRGLYKGLSPNLLK 404 >SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription termination factor Reb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 504 Score = 24.2 bits (50), Expect = 8.4 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +1 Query: 160 NVEPRRSGRVR*GLPGWXYLRRRLQD 237 N++P G R W YL RR+Q+ Sbjct: 145 NMDPFLKGSARWTAEHWDYLERRMQN 170 >SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces pombe|chr 2|||Manual Length = 229 Score = 24.2 bits (50), Expect = 8.4 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = -2 Query: 402 DDVGQVTTPE*KETLLLGDADEGVDDT 322 +D GQ TPE K+ LL + D + T Sbjct: 38 EDAGQFETPEDKDALLNFENDSEAEQT 64 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,518,767 Number of Sequences: 5004 Number of extensions: 24096 Number of successful extensions: 63 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 56 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 63 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 148351622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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