Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20144
         (507 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_A1YRL7 Cluster: Cationic peptide CP8; n=3; Bombyx|Rep: ...   142   3e-33
UniRef50_A4LA63 Cluster: Cationic peptide CP8; n=1; Manduca sext...   101   1e-20
UniRef50_A7S1Y8 Cluster: Predicted protein; n=1; Nematostella ve...    35   0.92 
UniRef50_UPI0000E4772C Cluster: PREDICTED: similar to cysteine-r...    34   1.6  
UniRef50_A7RYC6 Cluster: Predicted protein; n=1; Nematostella ve...    34   1.6  
UniRef50_UPI0000D56304 Cluster: PREDICTED: similar to CG3647-PA,...    33   2.8  
UniRef50_Q8MSX0 Cluster: LD16736p; n=1; Drosophila melanogaster|...    33   2.8  
UniRef50_UPI0000E81587 Cluster: PREDICTED: similar to connective...    33   3.7  
UniRef50_UPI0000DD7F3D Cluster: PREDICTED: hypothetical protein;...    32   6.5  
UniRef50_UPI0000E0EE6D Cluster: Tn7-like transposition protein D...    32   8.6  
UniRef50_Q4REY5 Cluster: Chromosome 13 SCAF15122, whole genome s...    32   8.6  

>UniRef50_A1YRL7 Cluster: Cationic peptide CP8; n=3; Bombyx|Rep:
           Cationic peptide CP8 - Bombyx mandarina (Wild silk moth)
           (Wild silkworm)
          Length = 89

 Score =  142 bits (345), Expect = 3e-33
 Identities = 60/67 (89%), Positives = 62/67 (92%)
 Frame = +1

Query: 73  SAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKCACCPACVTLLGEGATCKIYS 252
           +AAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKCACCPACVTLL EGATCKIYS
Sbjct: 4   AAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKCACCPACVTLLDEGATCKIYS 63

Query: 253 KN*AKPP 273
           K   + P
Sbjct: 64  KELGETP 70



 Score = 58.0 bits (134), Expect = 1e-07
 Identities = 25/26 (96%), Positives = 26/26 (100%)
 Frame = +3

Query: 252 EELGETPSAVCKEPLKCIKRVCTKLV 329
           +ELGETPSAVCKEPLKCIKRVCTKLV
Sbjct: 64  KELGETPSAVCKEPLKCIKRVCTKLV 89


>UniRef50_A4LA63 Cluster: Cationic peptide CP8; n=1; Manduca
           sexta|Rep: Cationic peptide CP8 - Manduca sexta (Tobacco
           hawkmoth) (Tobacco hornworm)
          Length = 105

 Score =  101 bits (241), Expect = 1e-20
 Identities = 42/88 (47%), Positives = 59/88 (67%), Gaps = 2/88 (2%)
 Frame = +1

Query: 31  MKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQP--PLVCPKNTEHRARHAGKCACCP 204
           MK ++ ++++AC  +  YG LVCG++YC+++PC  P     C   + +RA+HAGKCACCP
Sbjct: 1   MKAVVVLVVLACAMAVVYGDLVCGSNYCKQHPCGSPIAQSSCRSPSVYRAKHAGKCACCP 60

Query: 205 ACVTLLGEGATCKIYSKN*AKPPPLCVR 288
           ACVT+LGE A CK YSK   + P    R
Sbjct: 61  ACVTMLGENAACKTYSKELGETPSAICR 88



 Score = 45.6 bits (103), Expect = 7e-04
 Identities = 16/25 (64%), Positives = 23/25 (92%)
 Frame = +3

Query: 252 EELGETPSAVCKEPLKCIKRVCTKL 326
           +ELGETPSA+C++PLKC+  VCT++
Sbjct: 77  KELGETPSAICRDPLKCLNGVCTRV 101


>UniRef50_A7S1Y8 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 541

 Score = 35.1 bits (77), Expect = 0.92
 Identities = 15/41 (36%), Positives = 19/41 (46%), Gaps = 1/41 (2%)
 Frame = +1

Query: 94  VCGTDYCE-KNPCIQPPLVCPKNTEHRARHAGKCACCPACV 213
           VCG+D     NPC+     C  N     +H GKC   P C+
Sbjct: 141 VCGSDNVTYSNPCMLRSATCKSNGTITMKHRGKCGSSPRCM 181



 Score = 33.1 bits (72), Expect = 3.7
 Identities = 17/45 (37%), Positives = 21/45 (46%), Gaps = 6/45 (13%)
 Frame = +1

Query: 94  VCGTD-YCEKNPCIQPPLVCPKNTEHRARHAGKCA-----CCPAC 210
           VCG+D     NPC+    VC  N + R +H G C      C P C
Sbjct: 241 VCGSDGKTYDNPCVFKIAVCQMNGQLRLKHRGACGSRPDKCAPIC 285



 Score = 32.7 bits (71), Expect = 4.9
 Identities = 17/53 (32%), Positives = 23/53 (43%), Gaps = 2/53 (3%)
 Frame = +1

Query: 94  VCGTDYCE-KNPCIQPPLVCPKNTEHRARHAGKCACCPACVTLLGEGA-TCKI 246
           VCG+D     NPC+     C  N     +H GKC    +C     +G   CK+
Sbjct: 292 VCGSDNVTYSNPCMLRSATCKSNGTITMKHRGKCGSSQSCEQKKCKGTKVCKM 344


>UniRef50_UPI0000E4772C Cluster: PREDICTED: similar to cysteine-rich
           repeat-containing protein CRIM1; n=1; Strongylocentrotus
           purpuratus|Rep: PREDICTED: similar to cysteine-rich
           repeat-containing protein CRIM1 - Strongylocentrotus
           purpuratus
          Length = 1130

 Score = 34.3 bits (75), Expect = 1.6
 Identities = 25/66 (37%), Positives = 29/66 (43%)
 Frame = +1

Query: 31  MKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKCACCPAC 210
           MKT+IF+ LVA        AL CGT  CE+  C  P L C             C CC  C
Sbjct: 1   MKTIIFLSLVATAT-----ALSCGT--CEREKC--PTLSCQGGVVDDV-----CVCCKEC 46

Query: 211 VTLLGE 228
             L G+
Sbjct: 47  AKLDGQ 52


>UniRef50_A7RYC6 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 244

 Score = 34.3 bits (75), Expect = 1.6
 Identities = 24/72 (33%), Positives = 30/72 (41%)
 Frame = +1

Query: 13  VFKNFKMKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKC 192
           +  N  MK LI I L+ CV   A  AL C +  C+   C  PP  C             C
Sbjct: 144 ILLNTDMKLLITIALL-CVTLPALDALSCLS--CDMFECPPPPKDCKGGLAKDI-----C 195

Query: 193 ACCPACVTLLGE 228
            CC  C  ++GE
Sbjct: 196 GCCKVCAKVVGE 207


>UniRef50_UPI0000D56304 Cluster: PREDICTED: similar to CG3647-PA,
           isoform A; n=1; Tribolium castaneum|Rep: PREDICTED:
           similar to CG3647-PA, isoform A - Tribolium castaneum
          Length = 958

 Score = 33.5 bits (73), Expect = 2.8
 Identities = 18/52 (34%), Positives = 21/52 (40%)
 Frame = +1

Query: 64  CVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKCACCPACVTL 219
           C A+  Y  + CGT    K P    P   P+   H A HA     CP C  L
Sbjct: 592 CGANVLYPPIPCGT----KPPPCNNPCSRPRPCGHEANHACHTGACPPCTVL 639


>UniRef50_Q8MSX0 Cluster: LD16736p; n=1; Drosophila
           melanogaster|Rep: LD16736p - Drosophila melanogaster
           (Fruit fly)
          Length = 104

 Score = 33.5 bits (73), Expect = 2.8
 Identities = 21/59 (35%), Positives = 26/59 (44%)
 Frame = -3

Query: 268 VSPSSSNKSCKLHLHQVKLHKRDNKHTFQRVWRDAQCS*GKQVVAVCMDFFHSSLYHIP 92
           +  + S KSC LH       K D+ HT    +R A C  G+Q V    D  HS    IP
Sbjct: 14  IGSAGSGKSCLLHHFIESKFKDDSSHTIGVEFRLADCERGRQ-VGKATDMGHSRSGEIP 71


>UniRef50_UPI0000E81587 Cluster: PREDICTED: similar to connective
           tissue growth factor; n=1; Gallus gallus|Rep: PREDICTED:
           similar to connective tissue growth factor - Gallus
           gallus
          Length = 304

 Score = 33.1 bits (72), Expect = 3.7
 Identities = 22/64 (34%), Positives = 26/64 (40%)
 Frame = +1

Query: 37  TLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHAGKCACCPACVT 216
           T    +L+  +A    GA  C T  C+   C   PL CP  T H       C CC  C  
Sbjct: 7   TAAHTLLLLLLAPVWVGAQGC-TFPCQ---CPSQPLQCPAGTSH---VLDACGCCKVCAR 59

Query: 217 LLGE 228
            LGE
Sbjct: 60  QLGE 63


>UniRef50_UPI0000DD7F3D Cluster: PREDICTED: hypothetical protein;
           n=1; Homo sapiens|Rep: PREDICTED: hypothetical protein -
           Homo sapiens
          Length = 340

 Score = 32.3 bits (70), Expect = 6.5
 Identities = 19/54 (35%), Positives = 26/54 (48%)
 Frame = +1

Query: 7   RNVFKNFKMKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEH 168
           R+   N K   + F+ L +   S A+GA VC   +C K   +QP  VC K   H
Sbjct: 281 RSELHNRKPALVCFVALRSLPRSLAHGAEVCFRWFCVKQ--VQPVKVCRKRIRH 332


>UniRef50_UPI0000E0EE6D Cluster: Tn7-like transposition protein D;
           n=1; alpha proteobacterium HTCC2255|Rep: Tn7-like
           transposition protein D - alpha proteobacterium HTCC2255
          Length = 509

 Score = 31.9 bits (69), Expect = 8.6
 Identities = 16/60 (26%), Positives = 31/60 (51%), Gaps = 6/60 (10%)
 Frame = -3

Query: 406 RTQIIFRTD-----RDRSIASLYFP-ANENDYTSLVQTLLMHLRGSLHTAEGVSPSSSNK 245
           R  ++++ D     +  ++A +YFP  N      ++Q++     GS+HT  G++ SS  K
Sbjct: 60  RVSVVWKVDVEEIIKRHTLAPIYFPFLNTQQSAQVIQSMYSEQGGSIHTRAGIAASSVQK 119


>UniRef50_Q4REY5 Cluster: Chromosome 13 SCAF15122, whole genome
           shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
           Chromosome 13 SCAF15122, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 1215

 Score = 31.9 bits (69), Expect = 8.6
 Identities = 16/51 (31%), Positives = 22/51 (43%), Gaps = 6/51 (11%)
 Frame = +1

Query: 97  CGTDYCEKNPCIQPPLVCPKNTEHRARHAGKC------ACCPACVTLLGEG 231
           CG  YC     +   + C  +  H  + AG+C       CCP C + L EG
Sbjct: 623 CGVCYCYGGEVVCTRIPCYGDCSHPYKPAGQCCGECERTCCPVCDSCLYEG 673


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 488,171,986
Number of Sequences: 1657284
Number of extensions: 9640969
Number of successful extensions: 26714
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 25664
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26689
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 30528237263
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)